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RAHMAT GUNAWAN
Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman
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Journal : Journal Of Carbazon

 1 
Antioxidant Activity and Toxicity Tests of Panahan Leaf Extract (Ayapana triplinervis (Vahl) RM) against Shrimp Larvae ( Artemia salina Leach) Daniel, Daniel; Maygusten, Maygusten; Gunawan, Rahmat; Magdaleni, Agustina Rahayu
Journal of Carbazon Vol 1, No 2 (2023): December 2023
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jocarbazon.v2i1.35260

Abstract

Research on the antioxidant activity test and toxicity test of leaf extract (Ayapana triplinervis (ValH) RM) has been conducted against the larval shrimp (Artemia salina Leach). The dried leaves weighing 500 grams were macerated using methanol, filtered, and concentrated using a rotary evaporator. Methanol crude extract was fractionated with n-hexane, ethyl acetate, and methanol water. Based on Test Phytochemicals screening on secondary metabolites showed that the crude extract of methanol, n-hexane fraction, and methanol-water containing secondary metabolites alkaloids, flavonoids, and phenolic in ethyl acetate fraction of the content of secondary metabolites, alkaloids, and flavonoids, and the fraction of n-hexane and ethyl acetate contain steroids. In contrast, the crude extract of methanol and n-hexane fraction contains phenolic compounds. Test mortality of shrimp larvae (Brine Shrimp Lethality Test) showed mortality of shrimp larvae (Artemia salina Leach) using Probit analysis (SAS) to determine the value of 50% Lethal Concentration (LC50). In this test, the most active is the methanol crude extract of archery leaves, with a value of 18.8608 ppm, in a test of antioxidant activity using DPPH free radical reduction in the spectrophotometer and IC50 values obtained. In methanol crude extract was 430.73 ppm; n-hexane fraction was 168.5 ppm; ethyl acetate fraction was 114.87 ppm; and methanol-water fraction was 37.23 ppm
Co-Authors -, Bohari Adha, Dhea Nur Agustin Pratiwi, Agustin Agustinus Pata, Agustinus Aidil Putra, Aidil Aisyah, Ema Agustina Akbar, Muh. Ali Albar Alhabib, Albar Alimuddin - Allo, Veliyana Londong Aman Sentosa Panggabean Amanda, Rika Anta Wiguna, Alvilah Ardiansyah - Arif Maulana Yusuf Arissah, Nur Aristawidyaningrum, Apta Aspin Aspin, Aspin Awalludin, Dudi B.K, Felicia A. Stevanie` Badi'ah, Hanif Bakhri, Asep Samsul Boby Royan alfadlil, Boby Royan Bohari Yusuf Chairul Saleh Cynthia Linaya Radiman Daniel Daniel Darman, Darmansyah DARMANSYAH . Dedy Prasetyo Dewi Sundari, Dewi Eko Haryanto Elanda, Anggi Fadillah, Fahmi Fauziah, Holidah Fitria Fitria Giovanni, Krisno Hajarudin, Iik Hammamiyah, Ulinnuha Hanafie, Daffy Mohamad Ismail Harpinus, Doki Harvina, Harvina Herman, Heri Hermawan K. Dipojono Indaryono Indaryono, Indaryono Iswara, Muhammad Wahyugo Jahada, Jahada Jeki Purnama Putra Jumadhi, Nurkattho Kartika Pratiwi Kurniasih, Ririn Feby Kusumastuti, Meidina Lumaku, Samuel Pangestu Enggal Magdaleni, Agustina Rahayu Malfiany, Rini Malik, Abdilla Manik, Sondang Hukum Parulian Margarita, Margarita Marsella, Yuni Maryadi, Wendri Maygusten, Maygusten Meiniarti, Meiniarti Mokhamad Wahidin Muhamad Abdulkadir Martoprawiro Muhamad Yulianto Mukhamad Nurhadi, Mukhamad Nasri Nasri Nasri, Nasri Nindia, Azrina Noor Hindryawati, Noor Nurul Fatimah Onsardi, Onsardi pakasi, Resy Palentin, Ersa Rizkita Pardian, Riki Ananda Pratiwi Hutabarat, Ilona Puji Astuti Qosim, Assyfa Machmudah Romdona, Muhammad Zulfikar Rostiani, Yeny Sahirah, Nabilah Sinar Saibun Sitorus Said, Yahya Santoso, Muzi Sari, Prinka Susmita Sarwawan, Ade Putri Hawiyah Sihotang, Recha Defrosa Morasih Samta Slamet Supriyadi Solly Aryza Subagyono, R. R Dirgarini J.N. Subagyono, R.R. Dirgarini Subagyono, RR Dirgarini Julia Nurlianti Sudarijati Sudirman, Irpan Sugiarti - Suherman, Yahya Suparya, Eman Suyitno Suyitno Syafriyanti, Yuly Ubaedi, Ubaedi Utami, Devira Ulva Virginia, Bintang Wachdijono, Wachdijono Wahyudi, Habib Putra Wahyuni, Ade Putri Yasifa, Firhan Yudiana, Yudiana Yulianto, R. Mega Yuliyanto, R. Mega Yuliyanto, R.Mega Zain, Fikri Naufal