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Sutopo Hadi
Department Of Chemistry, Faculty Of Mathematics And Natural Sciences, Universitas Lampung, Bandar Lampung, 35145, Indonesia
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Journal : Emerging Science Journal

 1 
The Effect of Zeolite/Chitosan Hybrid Matrix for Thermal-stabilization Enhancement on the Immobilization of Aspergillus fumigatus α-Amylase Yandri Yandri; Hendri Ropingi; Tati Suhartati; John Hendri; Bambang Irawan; Sutopo Hadi
Emerging Science Journal Vol 6, No 3 (2022): June
Publisher : Ital Publication

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.28991/ESJ-2022-06-03-06

Abstract

In this paper, the A. fumigatus α-amylase had been immobilized onto zeolite/chitosan hybrid to improve its thermal-stabilization for industrial needs. The methods applied enzyme production, isolation, partial purification, immobilization, and characterization. The optimum temperatures of the native and immobilized enzymes were 50 and 55˚C, respectively. The native enzyme has KM of 3.478 ± 0.271 mg mL-1 substrate and Vmax of 2.211± 0.096 µmole mL-1 min-1, while the immobilized enzyme has KM value of 12.051 ± 4.949 mg mL-1 substrate and Vmax of 1.602 ± 0.576 µmole mL-1 min-1. The residual activity of the immobilized enzyme retained up 10.97% after fifth reuse cycles. The native enzyme has ΔGi of 104.35 ± 1.09 kJ mole-1 and t½ of 38.75 ± 1.53 min, while the immobilized enzyme has ΔGi of 108.03 ± 0.05 kJ mole-1 and t½ of 180.03 ± 3.31 min. According to the increase in half-life (t½), stability improvement of the A. fumigatusα-amylase was 4.65 times greater than the native enzyme. Thus, the zeolite/chitosan hybrid is used as a new supporting matrix for further enzyme immobilization to stabilize the enzymes. Doi: 10.28991/ESJ-2022-06-03-06 Full Text: PDF
Immobilization and Stabilization of Aspergillus Fumigatus α-Amylase by Adsorption on a Chitin Yandri Yandri; Ezra Rheinsky Tiarsa; Tati Suhartati; Bambang Irawan; Sutopo Hadi
Emerging Science Journal Vol 7, No 1 (2023): February
Publisher : Ital Publication

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.28991/ESJ-2023-07-01-06

Abstract

In this research, immobilization of A. fumigatus α-amylase on chitin was studied with the main purpose to improve the characteristics of the enzyme. A series of experiments were carried out to study stability improvement, thermodynamic parameters, include ki, ΔGi, and t½, and reusability of the immobilized enzyme. The experimental results indicate that significant thermal stability was achieved, as indicates by the ability of the enzyme to retain its relative activity above 39% after 80 min of incubation at 60oC. Thermodynamic parameters, include ki, ΔGi, and t½, indicate that the immobilized enzyme is more rigid, stable, and less flexible in the water, resulting in increased stability up to 1.5 times compared to that of the native enzyme. Furthermore, the immobilized enzyme was able to retain over 46% of its initial activity after six consecutive applications for starch hydrolysis, confirming the potential of chitin for the production of immobilized enzymes on an industrial scale. Doi: 10.28991/ESJ-2023-07-01-06 Full Text: PDF
Anticancer Activity Study of Modified Artocarpin Compound from Pudau Plant (Artocarpus kemando Miq.) Tati Suhartati; Khalimatus Sa’diah; Yandri Yandri; Sutopo Hadi
Emerging Science Journal Vol 7, No 3 (2023): June
Publisher : Ital Publication

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.28991/ESJ-2023-07-03-05

Abstract

This research is a continuation of the successful isolation of artocarpin from the root of Artocarpus kemando Miq reported in our previous study. In the previous study, the artocarpin was characterized with UV-Vis and FTIR techniques. In this follow-up investigation, the artocarpin was subjected to a transesterification reaction using acetic anhydride and pyridine as catalysts, and the product of the reaction was specified as compound 1. The compound 1 was further characterized with different techniques to gain more complete data and then tested for anticancer activity test against P-388 murine leukemia cells. Characterizations of the compound 1 using 1H-NMR and 13C-NMR techniques suggest that the modification reaction resulted in the conversion of the -OH groups at C2' and 4' at the artocarpin molecule to -OOCH3, and based on the MS analysis, the compound was proposed to have the molecular formula of C30H32O8. Another important feature of compound 1 that should be noted is the significant improvement in stability compared to the unmodified artocarpin. Anticancer activity tests against P-388 murine leukemia cells revealed that compound 1 has an IC50of 2.35 µg/mL, confirming that the compound is categorized as an active anticancer agent and suggesting that the compound has promising potential that deserves further investigations. Doi: 10.28991/ESJ-2023-07-03-05 Full Text: PDF
Co-Authors Afriyani, Hapin Ahmadi, Peni Ambarwati, Yuli Ambarwati, Yuli Andi Setiawan Andi Setiawan Arai, Masayoshi Aspita Laila Bambang Irawan Bambang Irawan C. N. Ekowati Dwi Asmi Ezra Rheinsky Tiarsa Hardoko Insan Qudus Hardoko Insan Qudus Hendri Ropingi Heri Satria Humairoh Ratu Ayu Indah Pratiwi Irvana, Raihan Irza Sukmana John Hendri John Hendri John Hendri Juliasih, Ni Luh Gede Ratna Junaidi Junaidi Karo , Pulung Karo Khalimatus Sa’diah Lailatul Farihah Lina Marlina Lutfiah, Rosyidatul Malik, Daffa Abdul Marjunus, Roniyus Mita Rilyanti Muhammad Rizki NANIK SUWARSO Ni Luh Gede Ratna Juliasih NOPIANI NOPIANI Nova Tri Irianti Noviany Noviany Nurul Nadila Pulung Karo-Karo Qudus, Hardoko Insan Ridho Nahrowi Setiawan, Fendi Suharso Suharso Sumardi Sumardi Supriyanto Supriyanto Suripto Dwi Yuwono Suripto Dwi Yuwono Suripto Dwi Yuwono Suripto Dwi Yuwono, Suripto Dwi Surtono, Arif Syaiful Bahri SYAIFUL BAHRI Tati Suhartati Tati Suhartati Tati Suhartati Tati Suhartati Tati Suhartati Tati Suhartati Trevor G Appleton Valennisa Qunifah Wasinton Simanjuntak Wasinton Simanjuntak WAWAN ABDULLAH SETIAWAN, WAWAN ABDULLAH YANDRI AS Yandri Yandri Yandri Yandri Yandri Yandri Yandri Yandri Yandri Yandri Yandri Yandri