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Hardian Hardian
Staf pengajar Bagian Fisiologi FK UNDIP, Jl. Dr. Sutomo No.18 Semarang
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Journal : Medica Hospitalia

 1 
Efektivitas Ozonated Virgin Coconut Oil Dosis Bertingkat Terhadap Penyembuhan Luka Full Thickness Skin Graft Autolog Ditinjau Dari Jumlah Fibroblas Dan Ekspresi Vascular Endothelial Growth Factor Pada Tikus Sprague Dawley Napitupulu, Johan Rinto Even; Hardian, Hardian; Najatullah, Najatullah
Medica Hospitalia : Journal of Clinical Medicine Vol. 8 No. 1 (2021): Med Hosp
Publisher : RSUP Dr. Kariadi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (747.524 KB) | DOI: 10.36408/mhjcm.v8i1.447

Abstract

Abstract Background: Now, Skin grafts are one of the therapies of choice in the wound healing process. And this is still developing today. The process of formation fibroblas and the formation of vascular endothelial growth factor have an important role in healing skin graft wounds. Ozonated virgin coconut oil has an important role in wound healing. Objective: To prove the effect of ozonated virgin coconut oil in various doses in increasing number of fibroblas and expression of vascular endothelial growth factor of skin graft wounds. Methods: This study is an experimental study with "Randomized parallel study with controlled group design" on 40 Sprague Dawley rats which are performed an autologous skin graft at the same time. Samples were divided randomly into 8 groups (K1 and K2 = without ozonated VCO), (A1 and A2 = ozonated VCO 50,4 mg/ml), (B1 and B2 = ozonated VCO 103,2 mg/ml), (C1 and C2 = ozonated VCO 204 mg/ml). Evaluation of the amount of fibroblas was done by staining hematoxylin & eosin after the 6th day and the expression of vascular endothelial growth factor was done by staining immunohistochemistry after the 12th day after skin graft. Results: Statistical analysis of the amount of fibroblas after the 6th day was found to be significantly different between the K1 vs A1 group (p = 0.029), K1 vs B1 (p = 0.004), K1 vs C1 (p = 0.000), on the 12th day was found significantly different the amount of fibroblast between K2 vs A2 ( p = 0,029 ), K2 vs B2 ( p = 0,010 ), K2 vs C2 ( p = 0,001 ). The expression of vascular endothelial growth factor on the 6th day was found a significant difference between the K1 vs A1 group (p = 0.024), K1 vs B1 (p = 0.005), K1 vs C1 (p = 0.001), on the 12th day was found significantly different the expression of vascular endothelial growth factor between K2 vs A2 ( p = 0,011 ), K2 vs B2 ( p = 0,036 ), K2 vs C2 ( p = 0,004 ) . Conclusion: Ozonated VCO can increase the amount of fibroblas and expression of vascular endothelial growth factor in the wound healing process of autologous skin graft in Sprague Dawley rats. Keywords: Ozonated VCO, full thickness skin graft, fibroblas, VEGF.
EFEKTIVITAS OZONATED VIRGIN COCONUT OIL TERHADAP PENYEMBUHAN LUKA FULL THICKNESS SKIN GRAFT AUTOLOG TIKUS SPRAGUE DAWLEY Ditinjau dari Ekspresi TGF-? dan Jumlah Neutrofil Kusuma, Nauval Marta; Hardian, Hardian; Najatullah, Najatullah; Yuniati, Renni; Susilaningsih, Neni
Medica Hospitalia : Journal of Clinical Medicine Vol. 8 No. 3 (2021): Med Hosp
Publisher : RSUP Dr. Kariadi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (710.499 KB) | DOI: 10.36408/mhjcm.v8i3.489

Abstract

Abstract Introduction : Skin grafts are now one of treatment option in wound healing process that is always developing. TGF-bexpression and the number of neutrophils have an important role in healing skin graft wounds. Ozone (O3) has disinfecting properties that are effective in wound healing. Objective : Proving the effectiveness of Ozonated VCO for Full Thickness Skin Graft wound healing using parameter of TGF-b expression and neutrophil number. Method : This study is an experimental study with a post-test only design group of 40 Sprague Dawley rats performed autologous skin graft at the same time. Samples were divided randomly into 8 groups (K1 = without Ozonated VCO), (A1 = Ozonated VCO 50 mg / ml), (B1 = Ozonated VCO 100 mg / ml), (C1 = Ozonated VCO 200 mg / ml), ( K2 = without Ozonated VCO) (A2 = Ozonated VCO 50 mg / ml), (B2 = Ozonated VCO 100 mg / ml), (C2 = Ozonated VCO 200 mg / ml). Assessment of TGF-b expression and neutrophil number of tissue was performed by staining hematoxylin & eosin and immunohistochemistry on days 6 and 12 after skin graft. Results : There were significant differences (p <0.05) TGF-b expression and neutrophils number of tissue between the control group and the administration of Ozonated VCO doses of 50 mg / ml, 100 mg / ml and 200 mg / ml on days 6 and 12 post skin graft. Conclusion : The administration of Ozonated VCO effectively improve Full Thickness Skin Graft wound healing seen from macroscopic wounds, increase TGF-b expression and decrease the number of neutrophils.
Co-Authors Ainun Rahmasari Gumay, Ainun Rahmasari Andani, Dea Fildatul Andi Alfia Muthmainnah Tanra Andreas Arie Setiawan, Andreas Arie Anggita, Vinka Dwi Annaafi, Alviona Denti Arief, Nadia Aris Sudiyanto Atmaja, Diana Auliya Husen Blezeinsky, Faradilla Nadya Budiwati, Septrina Chatarina Suharti Christian, Vika Adelia Dewi, Amalia Andansari Dini, Intan Rahmania Eka Dodik Tugasworo Dyan Yuliana Eka Dini, Intan Rahmania Eva Annisaa Farmaditya Eka Putra Fitriana, Anisa Desty Gery Rifano Hardanto Hertanto Wahyu Subagio Indah Saraswati Izziyana, Wafda Vivid Kusuma, Nauval Marta Kusumaningrum, Novi Lukman, Petrin Redayani Maharatih, Gusti Ayu Mochammad Imron Awalludin Mohammad Halilintar Muslimin Muslimin Nahwa Arkhaesi Najatullah, Najatullah Napitupulu, Johan Rinto Even Natalia Dewi Wardani Neni Susilaningsih Nurtari, Rahma Yulia Priguna, Tangkas Mukti Ragil Setia Dianingati Renni Yuniati Rika Maya Sari Rimbawan, Andhika Yuli Sagita, Tiffany Christine Selamat Budijitno Septiawan, Debree Suprapti, Rini Susilo, Kezia Natalia Daniast Widiastuti, Maria Immaculata