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All Journal Buletin Peternakan Jurnal Ilmu dan Teknologi Hasil Ternak Jurnal Sain Peternakan Indonesia Jurnal Ilmu Ternak Veteriner JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Indonesian Journal of Biotechnology Journal of Tropical Biodiversity and Biotechnology The Indonesian Biomedical Journal Livestock and Animal Research JITPI : Jurnal Ilmu Dan Teknologi Peternakan Indonesia (Indonesian Journal of Animal Science and Technology) Jurnal Agripet
Tety Hartatik
Faculty Of Animal Science, Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Public Health Veterinary Other

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PERUBAHAN FENOTIP SAPI PERANAKAN ONGOLE, SIMPO, DAN LIMPO PADA KETURUNAN PERTAMA DAN KETURUNAN KEDUA (BACKCROSS) Trifena (Trifena); I Gede Suparta Budisatria; Tety Hartatik
Buletin Peternakan Vol 35, No 1 (2011): Buletin Peternakan Vol. 35 (1) Februari 2011
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v35i1.585

Abstract

The aims of the study was to analyze the phenotype of Ongole Grade, SIMPO, and LIMPO cows based on the exterior characteristics and body size of cattle, and to distinguish the phenotype of the first filial (F1) and the backcross(BC1) of SIMPO and LIMPO cows, reared by farmers at Pacitan, East Java. The study was conducted with purposive sampling method, involving 5 districts: Pacitan, Arjosari, Pringkuku, Nawangan, and Punung. Eighty four farmers asrespondents with total of 100 head cattle were used in the study. The results showed that the dominant coat color in Ongole Grade was white. However, none of crossbreed in F1 and BC1 had white color, but the color phenotype changeto brown and dark brown, which increase the dark intensity. The muzzle color of Ongole Grade was black, and changed to red in BC1. The hoof color’s of Ongole Grade was black, and brown for BC1. The color phenotype of F1 was similarto both color of Ongole Grade and BC1 phenotype. The increasing intensity of coat color in F1 to BC1 indicated that there was a combination of gene from Simmental or Limousin with F1 and BC1which containing of two or several set ofgenes. SIMPO and LIMPO cows had greater body size than those of Ongole Grade cows. The body’s sizes of BC1 was larger than F1 in SIMPO and LIMPO cows, which was influenced by increasing the effect of additive genes from F1 toBC1.(Keywords: Ongole Grade, SIMPO, LIMPO, First filial, Backcross, Phenotype of cattle)
POLIMORFISME GEN GROWTH HORMONE (GH) PADA SAPI LIMURA (GROWTH HORMONE (GH) GENE POLYMORPHISM OF LIMURA CATTLE) Slamet Diah Vulkandari; Tety Hartatik; Sumadi (Sumadi)
Buletin Peternakan Vol 37, No 2 (2013): BULETIN PETERNAKAN VOL. 37 (2) JUNI 2013
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v37i2.2423

Abstract

The research was conducted to identify Growth Hormone (GH) olymorphism of Limura cattle. The research was conducted in the smallholder at Lancar, Montok and Duko village, Larangan subdistrict in Pamekasan district, East Java Province and Animal Breeding Laboratory of Faculty of Animal Science, Gadjah Mada University in April 2012. Then research used 35 Limura calves and 10 Madura calves (as control). Blood samples for DNA molecular analysis, i.e. DNA isolation with SDS-PK modification method, DNA amplification with PCR method and genotyping with RFLP method. Identification of GH gene polymorphism was conducted by digesting the DNA fragment of 211 bp extended from the fourthintron region (49 bp) to fifth of exon (162 bp) by AluI enzyme. The result indicated that GH gene of Madura cattle was not polymorphic with frequencies of L allele 1.00 and V allele 0.00. The LL genotype of Madura cattle was 1.00. Frequencies ofL and V allele in Limura cattle were 0.91 and 0.09, respectively. Limura cattle indicated polymorphic with genotype LL 0.83and LV 0.17. As a results, GH gene polymorphism was found in Limura cattle with L allele frequencies higher than V allele. Limura cattles population were not deviated from Hardy-Weinberg quilibrium genetic condition.(Key words: Growth Hormone Gene, Limura Cattle, Polymorphism)
ESTIMASI OUTPUT BABI DI KABUPATEN TABANAN PROVINSI BALI Bayu Dewantoro Putro Soewandi; Sumadi (Sumadi); Tety Hartatik
Buletin Peternakan Vol 37, No 3 (2013): BULETIN PETERNAKAN VOL. 37 (3) OKTOBER 2013
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v37i3.3088

Abstract

Tujuan penelitian ini adalah untuk membuat estimasi output pada babi Bali dan babi Landrace di Kabupaten Tabanan. Penelitian ini dilakukan di lima kecamatan di Kabupaten Tabanan dengan mengambil 150 peternak. Data yang diambil adalah identitas peternak, komposisi, dan reproduksi ternak babi Bali dan babi Landrace. Estimasi output dihitung dengan cara pendekatan teori pemuliaan. Hasil dari penelitian ini diketahui bahwa nilai natural increase babi Bali dan babi Landrace sebesar 60,93 dan 116,38%. Nilai net replacement rate jantan dan betina babi Bali dan jantan dan betina babi Landrace sebesar 7.664,29 dan 1.844,05% serta 15.033,33 dan 1.386,47%. Nilai output pada babi Bali jantan dan betina sebesar 30,78 dan 23,58% atau 360 dan 114 ekor serta jantan dan betina pada babi Landrace sebesar 45,57 dan 70,49% atau 6.722 dan 10.009 ekor. Kesimpulan dari penelitian ini adalah estimasi output pada babi Bali jantan (30,78%) lebih besar daripada babi Bali betina (23,58%), sedangkan pada estimasi output babi Landrace jantan (45,57%) lebih kecil daripada babi Landrace betina (70,49%).(Kata kunci: Estimasi output, Babi Bali, Babi Landrace)
ESTIMASI NILAI PEMULIAAN DAN MOST PROBABLE PRODUCING ABILITY SIFAT PRODUKSI SAPI ACEH DI KECAMATAN INDRAPURI PROVINSI ACEH Widya Pintaka Bayu Putra; Sumadi (Sumadi); Tety Hartatik
Buletin Peternakan Vol 38, No 1 (2014): BULETIN PETERNAKAN VOL. 38 (1) FEBRUARI 2014
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v38i1.4594

Abstract

Penelitian ini bertujuan untuk membuat estimasi nilai pemuliaan (NP) dan most probable producing ability (MPPA) sifat produksi sapi Aceh di Kecamatan Indrapuri, Provinsi Aceh. Penelitian dilaksanakan mulai tanggal 7 Maret 2013 sampai dengan 7 April 2013 di Balai Pembibitan Ternak Unggul (BPTU)-Hijauan Pakan Ternak (HPT) Sapi Aceh Indrapuri. Materi penelitian ini terdiri dari catatan produksi dan populasi sapi Aceh tahun 2010 sampai 2012. Jumlah sapi yang digunakan untuk mengestimasi NP dan MPPA pada sifat produksi adalah 38 ekor. Estimasi nilai estimasi heritabilitas dengan metode korelasi saudara tiri sebapak (paternal half-shib correlation), sedangkan estimasi repitabilitas berdasarkan dua catatan individu menggunakan metode korelasi antar kelas (interclass correlation). Hasil penelitian menunjukkan bahwa nilai heritabilitas berat lahir, berat sapih, berat satu tahun, dan berat akhir masing-masing adalah 0,15+0,12, 0,48+0,58, 0,49+0,58, dan 0,56+0,69. Nilai repitabilitas yang dapat diestimasi berdasarkan data recording adalah berat lahir sebesar 0,33+0,22. Sapi Aceh pejantan nomor P.075 memiliki NPabsolut berat akhir tertinggi yaitu sebesar 14,99 kg. Nilai MPPAabsolut berat lahir tertinggi pada induk sapi Aceh nomor A.0668 yaitu sebesar 15,38 kg.(Kata kunci: Sapi Aceh, NP, MPPA, Heritabilitas, Repitabilitas, Sifat produksi)
REPRODUCTIVE PERFORMANCES OF SUMBA ONGOLE COWS INSEMINATED WITH FROZEN BELGIAN BLUE SEMEN Riyan Nugroho Aji; Panjono Panjono; Ali Agus; Budi Prasetyo Widyobroto; Tety Hartatik; I Gede Suparta Budisatria; Ismaya Ismaya; Sigit Bintara
Buletin Peternakan Vol 41, No 4 (2017): BULETIN PETERNAKAN VOL. 41 (4) NOVEMBER 2017
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v41i4.22052

Abstract

The purpose of this study was to observe the reproductive performances of Sumba Ongole (SO) cows inseminated with frozen Belgian Blue (BB) semen. This research was expected to be used as a reference in the cow’s maintenance, especially in the implementation of Artificial Insemination (AI), as well as for subsequent studies. Parameter of this study includes the signs of estrus symptoms, S/C ratio, CR, NRR, and pregnancy time. The results showed a large value of S/C for cow SO 2.60±1.81, great value for cattle CR SO was 40%, the value of NRR was 60%, and the pregnancy time SO was 273.80±4.08. It is concluded that reproductive performance of SO cows are good, using SO cows to cross with BB cow is possible to do. 
SRY Gene Marker Differences in Native and Crossbreed Cattle Tety Hartatik; Dwi Ahmad Priyadi; Ali Agus; Sigit Bintara; I Gede Suparta Budisatria; Panjono Panjono; Budi Prasetyo Widyobroto; Yudi Adinata
Buletin Peternakan Vol 42, No 3 (2018): BULETIN PETERNAKAN VOL. 42 (3) AUGUST 2018
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v42i3.29940

Abstract

This study focused on the promoter region of the SRY gene with 1,281 bp DNA fragments, including 5'UTR, CAAT signal, and TATA box. Genomic samples of 19 cattle were obtained from Wagyu-BX (n = 2), BX (n = 5), Simmental (n = 2), Limousin (n = 2), Ongole (n = 2), Madura (n = 2), Bali (n = 2), Nellore (n = 1), and Hereford (n = 1). Two flanking primers (forward and reverse) were used for polymerase chain reaction (PCR). The PCR products were then sequenced by using a two-way primer. The obtained sequences were aligned with clustalW software to determine the differences in the nucleotide base arrangement which compiled the promoter region of the SRY gene. The cattle crossbreeding was done as an effort to improve the genetic variations and qualities. The SRY gene is a marker gene inherited from the male side (bull), so the SRY gene is expected to be used as a marker to monitor the crossbreeding. The monitoring of the crossbreed cattle is an initial effort to increase the genetic variations and enhance the genetic qualities without threatening the germplasm purity. The results of this study showed that the overall sample is monomorphic, except for Bali and Nellore cattle. Further research is needed by expanding the analysis area of the SRY gene and increasing the number of samples.
Morphometry of Male Reproductive Organ and Sperm Evaluation of Belgian Blue Crossbreed Cattle as a Candidate of Superior Bull Riyan Nugroho Aji; Panjono Panjono; Ali Agus; Budi Prasetyo Widyobroto; Tety Hartatik; I Gede Suparta Budisatria; Ismaya Ismaya; Akhmad Fathoni; Dwi Astuti; Sigit Bintara
Buletin Peternakan Vol 44, No 4 (2020): BULETIN PETERNAKAN VOL. 44 (4) NOVEMBER 2020
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v44i4.45944

Abstract

The purpose of this study was to observe the size of reproductive organ and to evaluate the sperm quality of Belgian Blue (BB) crossbreed cattle. This cattle are the crossbreeding result between a female Brahman Cross (BX) cattle inseminated with frozen semen from pure male BB cattle, by Artificial Insemination (IB) method, as a candidate of superior bull. The samples of this study were 2.5 years old (BB1), (BB2), and (BB3) crossbreed cattle. Its process involved general physical examination, genital organ inspection, sperm production, and quality assessment. The data obtained were analyzed through descriptive analysis. The results of this study were the three BB crossbred cattle showed the good appearance of normal reproductive organs with scrotum circumferences of each bull were 40.2 cm, 38.6 cm, and 39.8 cm respectively, and sperm quality of the good and the normal motility values of each bull were 82%, 81% and 80%, viability 84%, 83%, and 86%, and the sperm concentrations of each bull were 2.218 million/ml, 1.986 million/ml and 2.120 million/ml respectively. As a result, it could be concluded that those three BB crossbreed cattle are suitable for being used as a superior bull.
Single Nucleotide Polymorphism of Partial GDF9 Gene in Three Local Goat of Indonesia Compare with Several Goat in Asia Fatimah Az Zahra Chairunissa; Sigit Bintara; Tety Hartatik
Buletin Peternakan Vol 46, No 1 (2022): BULETIN PETERNAKAN VOL. 46 (1) FEBRUARY 2022
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v46i1.67188

Abstract

The GDF9 gene is a gene that affects the maturation of oocytes. GDF9 is expressed in oocytes and granulosa cell, it can stimulate granulose cell proliferation and regulate cumulus cell function from pre-ovulation to ovulation. The GDF9 gene is associated with an increase in the ovulation rate and litter size in animals. This study aims to determine the kinship relationship of local goats compared to goats in Asia on prolific traits and to determine the restriction mapping of the GDF9 gene in goats based on the different SNP locations. The local goat comes from the Bligon goat, Kacang goat and Kejobong goats which is compared to the GenBank data (EF446168, EU883989 and KY780296). GDF9 sequences were analyzed using BioEdit and sequencing results to identify Single Nucleotide Polymorphism (SNP) and using NEBCutter V2 to determine the restriction enzyme which recognized the sequence around SNP. The result shows that three variations of SNP were found in exon 2 (g.3615T>C, g.3760T>C and g.3855A>C). Identification of SNP position found 1 SNP position identified by restriction enzyme at g.3855A> C. The identified restriction enzyme is HpaII and MspI. The results of this study are expected to provide genetic information that will be used for further research on the relationship between GDF9 gene polymorphisms to animal prolific.
Body Weight Gain and Carcass Quality of the Hybrid Chicken Derived from the Crossing between Female F1 Kampung Super and Male F1 Kampung-Broiler Utin Elsya Puspita; Hendry T.S.S.G. Saragih; Tety Hartatik; Budi Setiadi Daryono
Journal of Tropical Biodiversity and Biotechnology Vol 6, No 2 (2021): August
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.60934

Abstract

This research was conducted to observe the body weight gain and carcass quality of the hybrid chicken derived from the crossing between female F1 Kampung Super and male F1 Kampung-Broiler. The weekly weight gain for seven weeks and carcass quality, including breast yield, protein content, lipid content, moisture, and pH were measured with each group consists of six individuals. The chickens were slaughtered for meat quality measurements on the 7th week. The results showed that the hybrid chickens’ weight at the 7th week (888.22±139.63) was higher than layer (467.06±85.7) and pelung (436.39±42.33). The hybrid chicken’s breast yield (3.20%) was significantly higher than pelung’s (1.66%). These characteristics of hybrid chicken breast muscle lead to the conclusion that hybrid chicken is a potential alternative meat-type chicken with local chicken meat characteristics and has relatively fast growth.
Restriction Mapping of MC4R Gene on Bali Cattle (Bos sondaicus) as Genetic Marker for Breeding Program in Compared to Bos taurus and Bos indicus Yoga Cipta Perdana; Tety Hartatik
Journal of Tropical Biodiversity and Biotechnology Vol 7, No 2 (2022): August
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.67636

Abstract

MC4R is a gene that has potential effects on growth traits such as body weight and feed intake. The usage of single nucleotide polymorphism (SNPs) in melanocortin-4 receptor (MC4R) as selection markers could help achieve effectiveness in the breeding program. This study aimed to analyze the restriction mapping based on SNPs in the MC4R gene for Bali cattle (Bos sondaicus) compared to various breeds of cattle. Partial MC4R gene was amplified using a primer (F: 5’-ACC AAT GTC AGT GAG TCC CC- 3’ and R: 5’-CTT CAT GTT GGC GCC CTG-3’) with a polymerase chain reaction (PCR) method. Genotype and allele frequencies were calculated using Chi-Square test and analyzed with Hardy–Weinberg law. Restriction enzyme was analyzed using Nebcutter V.2 to see the association between SNPs and the recognition site of restriction enzyme. The result showed four SNPs g.554 T>C, g.634 G>T, g.673 C>T, and g.742 G>A were found in the exon region. SNP g.742 G>A was found as a heterozygote genotype and the rest are SNP g.554 T>C, g.634 G>T, and g.673 C>T were found as homozygote genotypes. All SNPs were synonymous which did not change the amino acid translated. Three restriction enzymes were identified as MmeI, TspRI, and BsrI which attach to SNPs g.554 T>C, g.634 G>T, and g. 742 G>A respectively.  SNPs found notably g. 742 G>A can be used as genetic markers associated with growth traits for further research on Bali cattle. 
Co-Authors Agus Susilo Aji, Riyan Nugroho Akhmad Fathoni Ali Agus Ali Agus Ali Agus Bayu Dewantoro Putro Soewandi Budi Prasetyo Widyobroto BUDI SETIADI DARYONO Dhany Azharinto Mahardika Diah Tri Widayati Dwi Aris Agung Nugrahaningsih Dwi Astuti Eko Purnomo Endang Baliarti Fatimah Az Zahra Chairunissa Hartati (Hartati) Hartati . Hartati . Hendra Saumar Hendra Saumar Hendra Saumar Hendry T.S.S.G. Saragih I Gede Suparta Budisatria I Gede Suparta Budisatria I Gede Suparta Budisatria I Gede Suparta Budisatria Ismaya Ismaya Ismaya Ismaya Joko Riyanto Lies Mira Yusiati Nur Arfian Priyadi, Dwi Ahmad Resti Yuliana Rahmawati Ronny Martien S. Sumadi Sigit Bintara Slamet Diah Volkandari Slamet Diah Volkandari, Slamet Diah Slamet Diah Vulkandari Subandriyo . Sumadi (Sumadi) Sumadi . Sumadi . Sumadi Sumadi Sumadi Sumadi Sumadi Sumadi Sumadi Sumadi Tri Satya Mastuti Widi Trifena (Trifena) Utin Elsya Puspita Veronika Yuneriati Beyleto Wayan Tunas Artama Widodo Widodo Widya Pintaka Bayu Putra Widya Pintaka Bayu Putra Widya Pintaka Bayu Putra Widya Pintaka Bayu Putra Widya Pintaka Bayu Putra Widya Wasityastuti Yoga Cipta Perdana Yudi Adinata