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Characteristics of Crude Pepsin Enzyme from Catfish Stomach (Clarias sp.) Fatiranes, Erlando; Sidauruk, Santhy Wisuda; Hasan, Bustari
Aquatic Life Sciences Volume 2 Issue 2
Publisher : ETFLIN Publishing House

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Abstract

Fish stomach is a by-product of the fishing industry that has potential as a natural source of pepsin enzyme, particularly from catfish (Clarias sp.). This study aimed to characterize crude pepsin extracted from catfish stomachs. The extraction was carried out by homogenizing the stomach with 10 mM Tris-HCl buffer (pH 7.5), followed by centrifugation at 10,000 g for 15 min at 4°C. The obtained pepsinogen was activated using 3 N HCl at pH 2 and neutralized to pH 2.75 with 2 N NaHCO₃. Enzyme activity was determined using the hemoglobin assay at 280 nm, and protein concentration was measured by the Bradford method at 595 nm. The crude pepsin showed an activity of 33.50 ± 0.87 U/mL, protein concentration of 0.358 ± 0.005 mg/mL, total activity of 1,608 ± 41.57 U, and specific activity of 93.52 U/mg. The enzyme exhibited optimal activity at 50°C and pH 4, with relative activity toward NaCl, ZnCl₂, and FeCl₃ of 81.08%, 49.10%, and 128.15%, respectively, indicating Fe³⁺ acted as an activator. These results demonstrate that catfish stomachs can serve as a potential halal-compatible pepsin source, supporting enzymology advancement and fish waste valorization.
Co-Authors ', Desmelati ', Sahyudi ', Sakinah Abdul Salam Badji Adi Putra Silaban Aditya Rachman Aliunputra Alfajriey, Rayhand Aminah Abdullah Anak Agung Istri Sri Wiadnyani Ananda, Dea Andhra Syatapahana Ansyor Refinal Fadilah Nasution Ayu Agustina Ayu Agustina AYU WULANDARI Azura, Asyifa Siti Bella Kharisma Berkhaty, Ayang Putri Dahlia Dahlia Damayani, Noventa Dwi Debi Rachmadani Dedi Iskandar Deevrizal Deevrizal Desmelati ' Desmelati Desmelati Desmelati Desmelati Desmelati Desmelati Desmelati Desmelati Desnizarianti ' Dewita Dewita Dian Iriani Dya Surya Rantika Era Yuliana Evrin Septyanti Fajar Syukron Fatiranes, Erlando Febriana, Tika Feliatra Fitra Suzanti Ghazali, Tengku Muhammad Giffar Ali Firmansyah Gusti Putra Yunius Guswanda Putra Henry Robby Mustaqim Hutagaol, Debby Alvionita Ilham Azhari Nasution Indra Anggraini Ira Sari Irani, Dian Islami Hakim Karnila, Rahman Karnila Kembaren, Bella Billiant J Ananta M Andesba Arifin M Azwar M. Irvansyah, M. Irvansyah Malau, Chandra Yustitia Maskilin, Jippo Maulani, Iffah Mery Sukmiwati Mery Sukmiwati Mohd. Khan A. N Ira Sari N Ira Sari N. Ira Sari N. Ira Sari Nalurita Nalurita Nasib Naibaho, Nasib Nittaya Chaiyanate, Nittaya Nur Wahid, Nur Nurma Yunita Nurul Atasyah Atasyah Putra, Harifa Syah Putra, Muhammad Ridho Putri Alvin, Okthavia Putri, Desrili Niatami Qalbi Salim R Fathul Rahman Rahman, R. Fathul Ramadhan, Tengku Rahmat Reinaldy Pangestu Riana Silalahi Rika Sepia Alvianti Rinanda, Khairunnisa Yulia Rozi Rudy Irawan Ryan Nazmi Sahliana, Sahliana Sahyudi Sahyudi Santa Monika Santhy Wisuda Sidauruk Sastiwi Sastiwi Septyanti, Elvrin Shandy Shandy Sidik, Fawwaz Afifah Sihombing, Kevin Andreas Sri Aryati Rukmana Nasution Stefani Rani Audina Sukirno Mus Sumarto Sumarto Sumarto Sumarto Sumarto, Sumarto Syahrul Syahrul Tia Sundari Titania Tjandrawati Nugroho Tjipto Leksono Tjipto Leksono Tomy Andri Zulpa Trisla Warningsih Umi Habibah W Sidauruk, Santhy Yustiani, Maulida Zein, Fauzan Fadilah