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Journal : Mitra Sains

Patogenitas Edwarsiella tarda Pada Ikan Sidat (Anguilla marmorata) Selama Penyimpanan Beku -25°C Arsal Arsal; Asriani Hasanuddin; Ahmad Rizal
Mitra Sains Vol 4 No 3 (2016): Juli
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/mitrasains.v4i3.236

Abstract

The purpose of this study is to determine the patogenechity (ability to cause death) of the Edwarsiella tarda in fish eel (Anguilla marmorata) which have frozen and stored at a temperature of -25°C for 15, 25 and 35 days. Patogenecity was tested with methode by isolating E. tarda from the meat of frosen Eel that what stored for 15, 25 and 35 days ago carried infected to eels the size of 120-122 grams with injecting isolate bacteria Edwarsiella tarda is intraperitoneally as much as 0,1 ml/ind at a dose of 108 CFU/ml (standard 1 Mac Farland), then maintened and observed for 15 days. Pathogenicity test results based on mortality / death of the fish indicates that the bacteria Edwarsiella tarda taken from frozen eel fish stored for 15, 25 and 35 days at temperatures-25oC were still survive and still are pathogenic to fish eel with a tendency the longer the storage time, it will decrease the mortality rate / patogenitasnya. Results of analysis of variance showed mortality observed eel (Anguilla marmorata) with a storage time of 15 days and 25 days resulted in a significantly different effect than the storage time of 35 days with a storage temperature -25oC.
Profil Asam Amino Isi Burger Dari Kaldu Ikan Tongkol (Euthynnus affinis) Melalui Proses Pemanggangan Dan Pengukusan Finarti Finarti; Asriani Hasanuddin; Nasmia Nasmia
Mitra Sains Vol 6 No 1 (2018): Januari
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/mitrasains.v6i1.307

Abstract

This study aims to utilize the waste generated from the oil extraction process tuna burger as fill material and to determine the profile of amino acids by product burger filler material from the fish oil extraction process. The method used in this research was experimental research with t test to test the chemical composition (amino acids, water content, protein content, fat content, and ash content,), treatment with Steam and Bake. Each treatment was repeated two (2) times in order to obtain the number of experimental unit as much as 2 x 2 = 4 experimental units. The results of the amino acid profile of research in the treatment rose grilled with keseluruan total amino acid in the amount of 15.02% was the steamed at 7.27%. for the amino acid histidine to highest in steam treatment that is equal to 1.41% and 4.74% for the bake treatment, and the lowest is at 0:10 Methionine% at treatment steamed and grilled. For parameter prosimat highest water content of 43.08% in the treatment steamed while the lowest was in the amount of 24.59% in the treatment of roast. The highest protein content of 10.91% in the treatment roasted while the lowest is equal to 8.81% on a steam treatment. The highest fat content is equal to 1.63% on a steam treatment, while the lowest was 1.51% on a toasted treatment. The ash content of 3.92%, the highest in the treatment of roast and the lowest 2.88% on a steam treatment.