Garuda - Garba Rujukan Digital

ISOLASI DAN KARAKTERISASI YEAST AMILOLITIK DARI RAGI TAPE Nurhartadi, Edhi; Rahayu, Endang Sutriswati
Jurnal Teknologi Hasil Pertanian Vol 4, No 1 (2011)
Publisher : Universitas Sebelas Maret (UNS)

The demand of sugar increases every year caused by people growth. Indonesia has starchy materialslike tapioca and for long time used to produce food by traditional fermentation with ragi tape. Ragi tapecontains many amylolytic and fermentative microbial. The object of this study was to isolate amylolytic yeastfrom ragi tape, which could be used to produce amylolytic enzymes as an alternative source of amylolyticenzyme from microbial origin. Isolation was done by dilution and spread plate methods on peptone glucoseyeast extract soluble starch (PGYS) agar medium, incubated 30C, for 2 days and purified with same medium.The isolates were then identified based on morphology and physiology profiles. Selection of the strongest isolatewith high amylolytic potent was done with amylolytic activity. The chosen isolate was then used to produceamylolytic enzyme in yeast extract malt extract soluble starch (YMS) medium with variation of soluble starchcontent. 15 amylolytic yeast isolates have been isolated, and then identified with taxonomic keys were suspectedas Debaryomyces vanriji (4 isolates), Saccharomycopsis fibuligera (3 isolates), and Pichia burtonii (8 isolates).They could grow well on starch medium. The result of selection based on amylolytic activity showed that S.fibuligera E-3 was the strongest among the others. From the fermentation, this research obtained the cellscontent increased or obtained logarithmic phase in 1st day of fermentation, and after 2nd day reached stationaryphase medium. The reduction sugar content increased in 1st day of fermentation, after 2nd day was increasedslowly. The soluble starch content was decreased rapidly in 1st day, after 2nd day was decreased slowly. Theglucoamylase activity was high at 1st day, but in 2nd day was low. S. fibuligera had glucoamylase activity 44.13mg glucose/L/min/1 ml enzyme solution.

INKORPORASI MINYAK ATSIRI JAHE MERAH DAN LENGKUAS MERAH PADA EDIBLE FILM TAPIOKA Utami, Rohula; Kawiji, Kawiji; Nurhartadi, Edhi
Prosiding Seminar Biologi Vol 9, No 1 (2012): Seminar Nasional IX Pendidikan Biologi
Publisher : Prodi Pendidikan Biologi FKIP UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (347.39 KB)

ABSTRAK Tujuan dari penelitian ini untuk mengetahui aktivitas antioksidan minyak atsiri jahe merah dan lengkuas merah serta pengaruh inkorporasi minyak atsiri jahe merah dan lengkuas merah terhadap aktivitas antimikroba dan karakteristik sensori edible film tapioka. Aktivitas antioksidan diuji menggunakan metode DPPH. Pengujian aktivitas antimikroba dilakukan terhadap bakteri Pseudomonas putida dan Pseudomonas fluorescens sedangkan karakteristik sensori diuji dengan metode perbandingan jamak dan metode hedonik. Aktivitas antioksidan yang dihasilkan sebesar 16.61% untuk minyak atsiri jahe merah dan 22.22% untuk minyak atsiri lengkuas merah. Aktivitas antimikroba edible film dengan inkorporasi 0.1% minyak atsiri jahe merah mampu menghambat Pseudomonas putida sebesar 2.45 cm dan Pseudomonas fluorescens sebesar 1.92 cm sedangkan inkorporasi dengan minyak atsiri lengkuas merah masing-masing menghambat sebesar 2.35 cm dan 2.42 cm (diameter edible film 1.3 cm). Berdasarkan uji perbandingan jamak diketahui bahwa edible film dengan inkorporasi masing-masing 1% minyak atsiri mempunyai karakteristik yang masih mendekati edible film tanpa minyak atsiri dan juga disukai panelis. Oleh karena itu dengan hasil ini maka dapat dilakukan penelitian selanjutnya tentang aplikasi edible film ini sebagai pengemas aktif. Kata Kunci: edible film, jahe merah, lengkuas merah, minyak atsiri.

PEMBERDAYAAN UKM KERUPUK SINGKONG MELALUI DIVERSIFIKASI PRODUK Nurhartadi, Edhi; Muhammad, Dimas Rahadian Aji; Nursiwi, Asri
SEMAR (Jurnal Ilmu Pengetahuan, Teknologi, dan Seni bagi Masyarakat) Vol 4, No 2 (2016)
Publisher : LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/semar.v4i2.4572

Magelang regency is the center of cassava in Central Java. One of the villages in the subdistrict Kajoran whose people business are processing cassava into cassava crackers is Sidowangi village. Crackers processing is still done traditionally method. Equipment used were not conform with the standards for the food industry. It is known with the washing cassava peel in the form of a big tub filled with water, put peeled cassava, then washed by hand and foot. In order to clean cassava should be washed one by one so it takes a long time. Besides that the drying process is also done by uncontrolled because the sun drying with braids pedestal. If there is no sun the people can not drying the cassava crackers. Consequently crackers that have been damaged due to slicing become moldy or rotten and it can not be used. As an effort to resolve these problems, the UNS team has agreed with the SME partners to organize a program of activities: training and introduction liquid waste treatment technology into nata de cassava; Introductions production process technology mocaf as product diversification efforts; The introduction of appropriate technology cassava crackers processing food industry standards.The results show that the mplementation of the training activity making nata de cassava, Implementation of the training activity mocaf processing technology, Introduction Equipment For Improved Quality Cassava Crackers and Potential Product Diversification, entrepreneurial training and management of food industry, Pelatihan pemasaran berbasis IT dan strategi direct sellingKey words: diversification, crackers, cassava, introduction, technology

Karakterisasi bakteri pektinolitik dari limbah kulit jeruk dan karakterisasi pektinase yang dihasilkan serta studi aplikasinya untuk penjernihan sari buah Jeruk Pontianak Esti Widowati; Rohula Utami; Edhi Nurhartadi; Fenny Fenny
Journal of Tropical AgriFood Volume 2, Nomor 1, Tahun 2020
Publisher : Jurusan Teknologi Hasil Pertanian Universitas Mulawarman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35941/jtaf.2.1.2020.3937.34-44

PKejernihan merupakan salah satu parameter penting kualitas sari buah. Penelitian ini bertujuan untuk mengisolasi dan mengkarakterisasi bakteri pektinolitik dari limbah kulit jeruk. Dilakukan pula karakterissasi enzim pektin hidrolase ekstraseluler yang dihasilkan meliputi kondisi optimum untuk aktivitas dan kestabilannya, nilai KM dan Vmax. Pengaruh penambahan enzim pektin hidrolase ekstraseluler terhadap kekeruhan, viskositas, dan total padatan terlarut sari buah jeruk Pontianak juga diamati. Hasil dari penelitian didapatkan 10 isolat bakteri dengan karakter semua isolat bakteri merupakan Gram negatif, endospora negatif, dan katalase positif. Pada penjernihan sari buah jeruk Pontianak terpilih 3 isolat bakteri untuk karakterisasi enzim yaitu isolat A, H, dan I. Karakter enzim ditentukan berdasarkan pH optimum, suhu optimum, kestabilan pH dan suhu, serta nilai KM dan Vmaks. Enzim isolat A optimum pada pH 4 dan stabil pada pH 4-5; enzim isolat H optimum pada pH 4 dan stabil pada pH 3-4; enzim isolat I optimum pada pH 4-4,5 dan stabil pada pH 3-5. Ketiga enzim isolat optimum pada suhu 50oC dan stabil pada suhu 30-50oC. Nilai KM enzim isolat A, H dan I berturut-turut adalah 0,022; 0,011; 0,017 mg mL-1 dan nilai Vmaks berturut-turut adalah 0,041; 0,038; 0,039 U mL-1. Enzim pektin hidrolase ekstraseluler dari ketiga isolat tersebut dapat diaplikasikan pada penjernihan sari buah jeruk Pontianak.

Pengaruh Minyak Atsiri Jahe Merah dan Lengkuas Merah pada Edible Coating Terhadap Kualitas Fillet Ikan Patin Rohula Utami; Kawiji Kawiji; Edhi Nurhartadi; Muslika Kurniasih; Dedy Indianto
agriTECH Vol 33, No 4 (2013)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

The effects of edible coating enriched with red ginger and red galangal essential oil on the quality of patin fillets during refrigerated storage of 8 days were examined. fish quality determined was based on microbiological (Total Plate Count /TPc) and physicochemical (Total Volatile Bases/TVB, Thiobarbituricacid/TBa, pH, and color) quality. Treatment variation of patin fillets were essential oil concentration enriched in edible coating (0 %, 0.1%, 1%). The results indicated that both red ginger and red galangal essential oil could affect patin fish fillets quality. Essential oilenrichment edible coating will retain the patin fillets quality. In terms of microbial quality and TVB value, 1% essential oil of red ginger and red galangal enrichment in edible coating could extend shelf life of patin fillets for 2-4 days.ABSTRAKPenentuan pengaruh penambahan minyak atsiri jahe merah dan lengkuas merah dalam edible coating terhadap kualitas fillet ikan patin selama penyimpanan dingin dilakukan pada periode waktu 8 hari. Parameter kualitas ikan yang dianalisis adalah kualitas mikrobiologis (Total Plate Count/TPc), dan kualitas fisikokimia (Total Volatile Bases/TVB, Thiobarbituricacid/TBa, pH, dan warna). Variasi perlakuan fillet ikan patin yaitu konsentrasi minyak atsiri (0 %; 0,1%; 1%) yang ditambahkan dalam edible coating. Hasil penelitian ini mengindikasikan bahwa penambahan minyak atsiri baik jahe merah maupun lengkuas merah berpengaruh terhadap kualitas fillet ikan patin selama penyimpanan dingin. Penambahan minyak atsiri dalam edible coating mampu mempertahankan kualitas fillet ikan patin lebih baik dibandingkan perlakuan edible coating tanpa minyak atsiri. Berdasarkan kualitas mikrobiologis dan nilai TVB, perlakuan minyak atsiri jahe merah 1% dan minyak atsiri lengkuas merah 1% mampu meningkatkan umur simpan fillet ikan patin selama 2-4 hari.

Formulasi Pangan Darurat Berbentuk Food Bars Berbasis Tepung Millet Putih (Panicum milliaceum L.) dan Tepung Kacang Merah (Phaseolus vulgaris L.) Raden Baskara Katri Anandito; Siswanti Siswanti; Edhi Nurhartadi; Rini Hapsari
agriTECH Vol 36, No 1 (2016)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

This study aimed to obtain a formula emergency food in the form food bars made from white millet flour and red bean flour. Foodbars made with Intermediate Moisture Food (IMF) technology with wet dyeing technique. This study used completely randomized design (CRD), which consists of a single factor, namely the variation formula white millet flour and red bean flour. The results showed that the formula food bars with the highest level of consumer acceptance in the composition of 15 g of white millet flour, red bean flour 10 g, 2 g sugar, 10 g margarine, milk full cream 13 g, 6.043 g and the addition of water. In 100 g of food bars contained water, ash, protein, fat, carbohydrate, and caloric value a respectively of 16.45%, 1.45%, 10.99%, 35.39%, 42.26%, 0 , 81 and 233.80 kcallbar.ABSTRAKPenelitian ini bertujuan untuk memperoleh formula pangan darurat berbentuk food bars berbahan dasar tepung millet putih dan tepung kacang merah. Food bars dibuat dengan teknologi Intermediate Moisture Food (IMF) dengan teknik pencelupan basah. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari satu faktor yaitu variasi formula tepung millet putih dan tepung kacang merah (15:10; 12,5:12,5; 10:15). Hasil penelitian menunjukkan bahwa formula food bars dengan tingkat penerimaan konsumen tertinggi pada komposisi tepung millet putih 15 g, tepung kacang merah 10 g, gula halus 2 g, margarine 10 g, susu full cream 13 g, dan penambahan air 6,043 g. Dalam 100 g food bars terkandung air, abu, protein, lemak, karbohidrat nilai a dan kalori berturut-turut sebesar 16,45%,1,45%, 10,99%, 35,39%, 42,26%, 0,81 dan 233,80 kkallbar.

Fermentasi Whey Keju Menggunakan Biji Kefir (Kefir Grains) dengan Variasi Sumber Nitrogen Rohula Utami; Edhi Nurhartadi; Asri Nursiwi; Martina Andriani
agriTECH Vol 37, No 4 (2017)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Cheese whey is a waste product in cheese processing which contains 4–5% of lactose. Having high carbon source, this material could be a potential fermentation medium for kefir grains. During fermentation of kefir grains, alcohol, lactic acid and exopolysaccharide (kefiran) were produced. The kefir grains fermentation also affected by nitrogen source. Therefore, effects of different composition of lactose and nitrogen sources (including yeast extract, urea and mung bean sprouts extract) in the fermentation were examined in this study. The results showed that after 24 hours of incubation, kefir grains biomass reached 21.30-27.15 g (dry wt, g/250mL medium) from 25 g of intial kefir grains biomass. Fermentation broth showed pH values ranged from 3.49–3.94; alcohol level ranged from 0.11-0.31%; and lactic acid content ranged from 0.49–1.47%. The total of kefiran extracted from kefir grain and fermentation broth ranged from 0.63-1.76 g/L. This study indicated that cheese whey can be used as fermentation medium to produce alcohol, lactic acid and kefiran by kefir grains. The highest production of kefiran was achieved in the medium containing 1.2% of whey lactose and 4% of mung bean sprouts extract.Cheese whey is a waste product from cheese processing industry which still has lactose content of 4-5%, so that it can be used as a carbon source in the fermentation medium of kefir grains. During fermentation of kefir grains, alcohol, lactic acid and exopolysaccharide commonly called as kefiran were produced. The kefir grains fermentation also affected by nitrogen source. Furthermore, fermentation medium composition were varied at whey lactose concentration and nitrogen source composition (yeast extract, urea and mung bean sprouts extract) for kefir grains fermentation. The results showed that after 24 hours fermentation, kefir grains biomass ranged from 21.30-27.15 g; pH values ranged from 3.49-3.94; alcohol level ranged from 0.11-0.31%; lactic acid content ranged from 0.49-1.47%; and the total of kefiran ranged from 0.63-1.76 g/L. Fermentation medium composition that produced the higher total of kefiran was 1,2% whey lactose and 4% mung bean sprouts extract as nitrogen source. ABSTRAKWhey keju adalah produk sampingan dari industri pengolahan keju yang masih memiliki kandungan laktosa sebesar 4–5% sehingga dapat dimanfaatkan sebagai sumber karbon pada media fermentasi oleh biji kefir. Selama proses fermentasi menggunakan biji kefir terjadi pembentukan alkohol, asam laktat dan eksopolisakarida (kefiran). Sumber nitrogen juga dapat mempengaruhi proses fermentasi. Oleh karena itu, dalam penelitian ini dilakukan variasi perlakuan, yaitu variasi konsentrasi laktosa pada whey dan variasi komposisi sumber nitrogen (yeast extract, urea dan ekstrak tauge kacang hijau) sebagai media fermentasi oleh biji kefir. Setelah fermentasi biji kefir selama 24 jam, jumlah biomassa biji kefir berkisar antara 21,30–27,15 g (berat kering g/250 mL medium fermentasi) dari jumlah biomassa awal 25 g. Cairan fermentasi menunjukkan nilai pH berkisar antara 3,49–3,94; kadar alkohol berkisar antara 0,11–0,31% dan kadar asam laktat berkisar antara 0,49–1,47%. Total rendemen kefiran yang didapatkan dari ekstraksi biji kefir dan cairan fermentasi berkisar antara 0,63–1,76 g/L. Hasil penelitian ini mengindikasikan bahwa whey keju dengan penambahan sumber nitrogen dapat digunakan sebagai medium fermentasi oleh biji kefir untuk menghasilkan metabolit berupa alkohol, asam laktat dan kefiran. Variasi formulasi media fermentasi yang menghasilkan kefiran dalam jumlah tertinggi adalah penggunaan konsentrasi laktosa whey keju 1,2% dan sumber nitrogen dari ekstrak tauge kacang hijau sebesar 4%.

INKORPORASI MINYAK ATSIRI JAHE MERAH DAN LENGKUAS MERAH PADA EDIBLE FILM TAPIOKA Rohula Utami; Kawiji Kawiji; Edhi Nurhartadi
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 9, No 1 (2012): Prosiding Seminar Nasional IX Biologi
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (347.39 KB)

ABSTRAK Tujuan dari penelitian ini untuk mengetahui aktivitas antioksidan minyak atsiri jahe merah dan lengkuas merah serta pengaruh inkorporasi minyak atsiri jahe merah dan lengkuas merah terhadap aktivitas antimikroba dan karakteristik sensori edible film tapioka. Aktivitas antioksidan diuji menggunakan metode DPPH. Pengujian aktivitas antimikroba dilakukan terhadap bakteri Pseudomonas putida dan Pseudomonas fluorescens sedangkan karakteristik sensori diuji dengan metode perbandingan jamak dan metode hedonik. Aktivitas antioksidan yang dihasilkan sebesar 16.61% untuk minyak atsiri jahe merah dan 22.22% untuk minyak atsiri lengkuas merah. Aktivitas antimikroba edible film dengan inkorporasi 0.1% minyak atsiri jahe merah mampu menghambat Pseudomonas putida sebesar 2.45 cm dan Pseudomonas fluorescens sebesar 1.92 cm sedangkan inkorporasi dengan minyak atsiri lengkuas merah masing-masing menghambat sebesar 2.35 cm dan 2.42 cm (diameter edible film 1.3 cm). Berdasarkan uji perbandingan jamak diketahui bahwa edible film dengan inkorporasi masing-masing 1% minyak atsiri mempunyai karakteristik yang masih mendekati edible film tanpa minyak atsiri dan juga disukai panelis. Oleh karena itu dengan hasil ini maka dapat dilakukan penelitian selanjutnya tentang aplikasi edible film ini sebagai pengemas aktif. Kata Kunci: edible film, jahe merah, lengkuas merah, minyak atsiri.

Characteristic and Self-Life Test of Food Bar with Combination of White Millet, Snakehead Fish and Soy Flour Lukita Purnamayati; Raden Baskara Katri Anandito; Siswanti Siswanti; Edhi Nurhartadi
Caraka Tani: Journal of Sustainable Agriculture Vol 34, No 1 (2019): April
Publisher : Universitas Sebelas Maret

Emergency food is needed when a disaster occurs. Emergency conditions encourage the provision of food that is ready to eat with sensory properties that can be accepted by the community, has sufficient nutrient content to meet the body's caloric needs and is resistant during storage. This study aims to obtain the best formulas of the food bar based on the parameters observed, namely sensory, chemical, total caloric, and aw as well as its shelf life estimation with the Accelerated Shelf Life Test. Food bar was made from white millet flour and fish koya that was made from snakehead fish and soy flour. Intermediate moisture food (IMF) applied in this study with the initial formulation was determined using mass equilibrium. The effectiveness test was used to determine the best formula based on all observed parameters. The results showed that the best food bar formula was formula F2, which has a chemical composition consisting of moisture content (20,99±0,21%); ash (3,45±0,35%); fat (18,10±0,13%); protein (12,24±0,28%); and carbohydrates (45,22±0.32%), aw (0,76±0,01); and total calories (per 50 g) was 232,04±1,96 kcal. The shelf life of food bar F2 was 19 days. Based on the results obtained, the total calories produced from the best formula have not met emergency food calorie standards.

INTRODUKSI PROSES PENGOLAHAN PANGAN BERBASIS TANAMAN HORTIKULTURA UNTUK PEMBERDAYAAN MASYARAKAT DESA WUNUT KABUPATEN KLATEN Danar Praseptiangga; Siswanti Siswanti; Edhi Nurhartadi
SEMAR (Jurnal Ilmu Pengetahuan, Teknologi, dan Seni bagi Masyarakat) Vol 6, No 1 (2017)
Publisher : LPPM UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/semar.v6i1.20894

Wunut Village is located in Tulung Sub-District, Klaten Regency which has the horticultural potential such as tomato and chili. But the huge potential has not been able to lift the economy of the village community. Therefore the community devotion team of Department of Food Science and Technology, Faculty of Agriculture, Sebelas Maret University chose Wunut Village as the target village. The problems faced by farming communities in Wunut Village are (1) production of chili and tomatoes are abundant, but the selling price is relatively low; (2) the large number of poor people; (3) lack of knowledge and community insight about entrepreneurship in the field of food; (4) interest in entrepreneurship is still lacking in society; And (5) the difficulty of marketing the resulting product. The solutions offered by the community devotion team are through training activities and the introduction of tomato processing technology into “torakur”; Training and introduction of production process technology of shredded pepper; Entrepreneurship training and food industry management; Information technology-based marketing training and direct selling strategies. In order to support these activities, aids were also provided to improve the quality of the chakra and the resulting chili pepper. Equipment provided in the form of cabinet dryer, dry mill, and packer sealer. The result of this activity in the form of introduction of tomato processing technology into “torakur”, and processing of chili into shredded chili has been done well, with products that can be accepted by society and have a big prospect to be developed.Keywords:entreprenuership, quality, torakur, shredded chili, Wunut Village

Title

Found 25 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 25 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 25 Documents
Search