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KANDUNGAN PROKSIMAT, ASAM SIANIDA, ASAM FITAT DAN TEKSTUR TAHU KEDELAI (Glycine max)-KORO PEDANG PUTIH (Canavalia ensiformis) DENGAN PENGGUMPAL ASAM ASETAT Ishartani, Dwi; Affandi, Dian R.; Amanto, Bambang S.; Rahayu, Puji P.
Jurnal Teknologi Hasil Pertanian Vol 12, No 1 (2019): Februari
Publisher : Universitas Sebelas Maret (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (373.086 KB) | DOI: 10.20961/jthp.v12i1.37300

Abstract

Tujuan penelitian ini adalah mengetahui pengaruh konsentrasi asam asetat sebagai penggumpal terhadap kandungan proksimat, asam sianida, asam fitat dan tekstur tahu kedelai-koro pedang. Penelitian ini menggunakan Rancangan Acak Lengkap satu faktor yaitu konsentrasi asam asetat (0,20%; 0,25%; dan 0,30%). Sampel disiapkan dalam tiga ulangan dan data yang dihasilkan dianalisis secara statistic menggunakan One Way ANOVA yang dilanjutkan dengan DMRT (α:5%) jika terdapat beda nyata. Hasil penelitian menunjukkan bahwa peningkatan konsentrasi asam asetat menghasilkan tahu dengan kadar asam sianida dan F max yang semakin tinggi tetapi kadar air dan abu yang semakin rendah. Konsentrasi asam asetat tidak berpengaruh terhadap kadar protein, lemak, dan asam fitat tahu kedelai-koro pedang putih yang dihasilkan.
PEMURNIAN PROTEASE DARI BUAH DAN DAUN MENGKUDU (Morinda citrifolia L.) [Purification of Proteases from Fruits and Leaves of Noni (Morinda citrifolia L.)] Dwi Ishartani; . Elfi; Nuri Andarwulan; Dahrul Syah
Jurnal Teknologi dan Industri Pangan Vol. 22 No. 1 (2011): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (491.63 KB)

Abstract

Proteases have been widely used in cancer treatment, wounds healing, overcoming digestion disorder and other modern pharmaceutical applications. Proteases may be present in fruits and leaves of noni (Morinda citrifolia L.) since the plant has been used traditionally in wound healing. This research aimed to purify proteases from noni’s fruits and leaves at two maturity stages, i.e. fruits with green-yellow (TK2) and white-yellow (TK4) skin colour, also leaves from shoot and base. Proteases were purified through several steps consisting of extraction, precipitation using saturated ammonium sulfate and dialysis followed by electrophoresis under denaturing conditions (SDS-PAGE) and zymography. The specific activity of the four extracts showed different trend during purification. The specific activity of TK2 fruit, shoot and base leaves decreased whereas TK4 fruits increased. TK2 crude extract had a higher specific activity (3.79 U/mg) than the other crude extracts. SDS-PAGE and zymogram using 12% acrylamide indicated that the dialysates were not pure proteases. The molecular weight profiles of the TK2 dialysates were similar to TK4 dialysates, while those of shoot leaves dialysates were similar to the base leaves dialysates. The SDS-PAGE separated the enzymes in the fruit dialysates into several bands of polypeptides,i.e. 24-26kDa, 14-15kDa, 12-13kDa and the smaller ones, and also separated the enzymes in the dialysates of leaves into two bands, 70kDa and 58-61.5kDa. Protease bands detected in the zymogram of fruits were estimated at ~25kDa, ~27kDa, and 37-38kDa, whereas of those of leaves were estimated at ~29kDa and ~50kDa. The ~25kDa and ~29kDa protein bands appearing in zymogram were similar to papain’s and bromelain’s molecular weight.
PHYSICAL CHARACTERISTICS, SENSORY EVALUATION, AND AMINO ACID CONTENT OF POWDERED OVER FERMENTED MLANDING TEMPEH Asri Nursiwi; Dwi Ishartani; Ardhea Mustika Sari; Nur Ayu Istiqomah
Jurnal Teknologi dan Industri Pangan Vol. 32 No. 2 (2021): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.6066/jtip.2021.32.2.190

Abstract

Mlanding tempeh is non-soybean tempeh made from ripe lamtoro (Leucaena leucocephala) seed. In Wonogiri, an area in Indonesia, over fermented mlanding tempeh (OFMT) is often used as a seasoning for traditional food since it produces a special taste. OFMT is also dried to extend the shelf life, making it easier to use as a seasoning. This research aims to study the physical characteristics, sensory evaluation, and amino acid content of powdered over fermented mlanding tempeh (POFMT). The drying of the OFMT used a cabinet dryer with variations in drying temperatures of 55, 60, and 65°C for eight hours. Then, it was mashed and sifted to obtain POFMT. The obtained powder was then analyzed for its physical characteristics in yield, water absorption capacity, solubility, bulk density, sensory evaluation with the hedonic test, and amino acid content. This study found that different drying temperatures significantly affected the yield and water absorption but did not significantly affect the solubility and bulk density. The yield values varied between 7.06-12.92%; water absorption capacity varied between 0.36-0.55 g/g; solubility varied between 36.45-41.33%; bulk density varied between 0.38-0.42 g/mL. The sensory evaluation results showed that the panelists' preference for POFMT with variations in drying temperature was not significantly different. In addition, the amino acid content of POFMT varied between 0.99-5.43% (db), with the highest levels being glutamic acid and aspartic acid.
PENGARUH MADU TERHADAP KARAKTERISTIK FISIK, KIMIA, DAN MIKROBIOLOGI VELVA JAMBU BIJI MERAH (Psidium guajava L.) PROBIOTIK (Lactobacillus acidophilus IFO 13951) Mandasia Pangastuti; Dwi Ishartani; Rohula Utami; M. Zukhrufuz Zaman
AGROINTEK Vol 14, No 2 (2020)
Publisher : Agroindustrial Technology, University of Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrointek.v14i2.6166

Abstract

This study was processed pink guava becomes probiotic’s velva as a functional food which safe to be consumed by lactose intolerance. The use of honey as a substitute for sucrose is expected to minimize calorie content, increase functional value, and maintain the viability of LAB in velva. The aim of this study was to determine the effect of honey on velva’s characteristics (total soluble solids, overruns, melting rate, and total calories), to determine the effect of honey on velva’s characteristics (pH, titratable acidity, and total LAB) during storage, and to determine the effect of honey on velva’s vitamin C levels and antioxidant activity after 6 weeks storage. There were 4 treatments which consist of F0 25% sucrose (control), F1 15% honey, F2 20% honey, and F3 25% honey. This study used Randomized Block Design. Data was analyzed using One Way ANOVA. The results showed that variations in honey concentrations significantly affect the characteristics of the velva (total soluble solids, overruns, melting rate, and total calories), affecting the pH value and total LAB during storage, and affecting vitamin C levels and antioxidant activity after storage. The higher the concentration of honey, the higher the value of total soluble solids, melting rate, total calories, vitamin C levels, antioxidant activities, and was effective at preserving the viability of LAB, while the value of overrun, and pH is lower.
PERBAIKAN KEMASAN UNTUK MENINGKATKAN NILAI JUAL SOSIS SOLO DI UKM SOSIS GAJAHAN [PACKAGING IMPROVEMENT TO INCREASE SELLING VALUE OF SOSIS SOLO IN SMALL AND MEDIUM ENTERPRISE (SME) SOSIS GAJAHAN] Asri Nursiwi; Dwi Ishartani; Siswanti Siswanti; Ardhea Mustika Sari
Jurnal Sinergitas PKM & CSR Vol 4, No 1 (2019): October
Publisher : Universitas Pelita Harapan

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Sosis solo is a traditional food from Solo which is made from an omelette with meat filling. Sosis solo usually served as snacks in various events. In Solo there are many Small and medium enterprise (SME) producing sosis solo, one of them is SME Sosis Gajahan.  The SME has been developing and know the opportunity that sosis solo as a typical food from Solo, has the potential to be developed as typical Solo gift. However, there are problems faced by these SME, namely 1) the packaging is not attractive and has not been labeled in accordance with the requirements of the food industry and 2) the shelf life of sosis solo is short, less than 24 hours. The aims of the community service activity were to improve the packaging of the sosis solo so that increasing the seelling value. To overcome these problems, had been did 1) improvement of packaging and labeling, and 2) introduction of vacuum packaging to package the sosis solo so that the shelf life is longer. The results obtained from the activity are the better new packaging designs for sosis solo and more attractive so that it increasing the selling value of sosis solo. Sosis solo packaged with a vacuum method followed by cold storage has longer shelf life. It has an impact on wider marketing reach.
Pendampingan Ukm Tempe Mlanding Di Desa Pesido, Kecamatan Jatiroto, Wonogiri Dwi Ishartani; Ardhea Mustika Sari; Asri Nursiwi; M. Zukhrufuz Zaman
Prosiding Konferensi Nasional Pengabdian Kepada Masyarakat dan Corporate Social Responsibility (PKM-CSR) Vol 2 (2019): Peran Perguruan Tinggi dan Dunia Usaha dalam Mempersiapkan Masyarakat Menghadapi Era I
Publisher : Asosiasi Sinergi Pengabdi dan Pemberdaya Indonesia (ASPPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (407.354 KB) | DOI: 10.37695/pkmcsr.v2i0.449

Abstract

Tempe mlanding merupakan makanan fermentasi tradisional dari biji lamtoro atau sering disebut mlanding oleh masyarakat Jawa Tengah. Produk pangan berbahan baku lokal ini berpotensi sebagai sumber protein selain kedelai. Di Desa Pesido, Jatiroto, Kabupaten Wonogiri, terdapat beberapa pengrajin tempe mlanding diantaranya adalah usaha tempe mlanding milik Ibu Lati Winarsih dan Ibu Tarti. Secara umum masalah yang terjadi pada proses pembuatan tempe mlanding adalah proses yang masih tradisional sehingga belum ada standar atau pengukuran parameter sehingga kualitas produk tidak konsisten. Sebagai upaya untuk menyelesaikan masalah tersebut, tim pengabdian UNS telah menyelenggarakan tiga program yaitu introduksi teknologi tepat guna proses pengolahan tempe mlanding standar industri pangan, pelatihan dan penyususnan Cara Pengolahan Pangan yang Baik (CPPB). Penggunaan rak fermentasi sebagai pengganti fermentasi yang biasa dilakukan di lantai beralaskan kain terpal merupakan teknologi tepat guna yang diaplikasikan pada UKM tempe mlanding sehingga suhu fermentasi lebih stabil. Pelatihan dan penyusunan konsep CPPB dilakukan kepada pemilik UKM melalui praktik langsung. Dengan ketiga program tersebut, pemilik UKM menjadi lebih paham tentang teknik fermentasi dan produksi pangan yang sesuai standar sehingga meningkatkan konsistensi produk.
PENINGKATAN KAPASITAS DAN MUTU PRODUK DI IRT ANEKA SNACK BU MARNI Siswanti, Siswanti; Ariviani, Setyaningrum; Fauza, Gusti; Muhammad, Dimas Rahadian Aji; Ishartani, Dwi; Atmaka, Windi
Fruitset Sains : Jurnal Pertanian Agroteknologi Vol. 11 No. 6 (2024): February
Publisher : Institute of Computer Science (IOCS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35335/fruitset.v11i6.4881

Abstract

Industri Rumah Tangga (IRT) “Aneka Snack Bu Marni” telah beroperasi selama 2 tahun dengan kegiatan bisnis berupa produksi beragam makanan ringan atau snack (pangsit goreng, kue unca, kerak nasi, dll.). Permintaan produk meningkat beberapa waktu terakhir, namun kapasitas produksi rata – rata terbatas pada 30 kg/bulan untuk pangsit goreng, 15 kg/bulan untuk kue unca, dan 5kg/bulan untuk kerak nasi. IRT ini menghadapi beberapa kesulitan seperti terbatasnya kapasitas pembuatan adonan hingga alat penggorengan yang tidak memadai. Brand awareness masyarakat terhadap produk IRT ini juga masih rendah karena kurangnya pengetahuan pemasaran produk oleh pemilik serta karyawan. Tim pengabdian masyarakat dari Universitas Sebelas Maret memilih IRT ini sebagai mitra untuk di bantu dalam penanggulangan beberapa isu tersebut melalui introduksi teknologi tepat guna. Kegiatan pengabdian masyarakat ini bertujuan untuk meningkatkan kapasitas produksi, menjamin kualitas produk, dan meningkatkan pemasaran produk di IRT Aneka Snack Bu Marni melalui introduksi-pelatihan beragam alat produksi (dough sheeter, kompor, fry-pan, dan sealer), introduksi meja stainless steel sebagai tempat bekerja, pelatihan pengemasan dan pembubuhan label produk serta pelatihan digital marketing. Kegiatan pengabdian masyarakat tersebut berhasil meningkatkan kapasitas produksi per hari (±30%), kualitas produk (elastisitas-homogenitas adonan dan pengemasan-label produk), nilai penjualan (±30%), dan jangkauan pemasaran (±2 kecamatan)
KARAKTERISTIK KIMIA, FISIK DAN TINGKAT KESUKAAN PANELIS PADA SNACK BAR TEPUNG EDAMAME (Glycine max (L.) Merr.) DAN TEPUNG KACANG HIJAU (Vigna radiata) DENGAN PENAMBAHAN FLAKES TALAS (Colocasia esculenta) Kurniawan, Leonardo Kevin; Ishartani, Dwi; Siswanti, Siswanti
Jurnal Teknologi Hasil Pertanian Vol 13, No 1 (2020): Februari
Publisher : Universitas Sebelas Maret (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (419.205 KB) | DOI: 10.20961/jthp.v13i1.36096

Abstract

KAJIAN SIFAT SENSORIS, FISIK DAN KIMIA POUND CAKE SUBSTITUSI TEPUNG LABU KUNING (Cucurbita moschata) TERMODIFIKASI ASAM ASETAT Mayasari, Anastasia; Ishartani, Dwi; Siswanti, Siswanti
Jurnal Teknologi Hasil Pertanian Vol 10, No 1 (2017)
Publisher : Universitas Sebelas Maret (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (578.201 KB) | DOI: 10.20961/jthp.v10i1.19158

Abstract

This research aimed to find out sensory, physical and chemical characteristics of pound cake substituted by acetic acid modified pumpkin flour. In this research, sensory characteristics of pumpkin studied included color, taste, texture and overall parameters. Physical characteristics included water level, β-carotene level, and coarse fiber level.  The result of research showed that pound cake substituted by acetic acid modified pumpkin flour affected the sensory (color, taste, texture and overall) characteristics. Pound cake with acetic acid modified pumpkin flour substitution of 20% was preferred to the one with other substitution. The use of acetic acid modified pumpkin flour improved yellow color intensity, hardness (Fmax) but lowered expandability of pound cake, increased water level, β-carotene level and coarse fiber level of pound cake.
KARAKTERISTIK FISIKOKIMIA TEPUNG KACANG TURI BERBUNGA PUTIH (Sesbania grandiflora) DENGAN BEBERAPA PERLAKUAN PENDAHULUAN Ishartani, Dwi; Affandi, Dian Rachmawanti; Purnamasari, Dessy Cahya
Jurnal Teknologi Hasil Pertanian Vol 7, No 2 (2014)
Publisher : Universitas Sebelas Maret (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (64.926 KB) | DOI: 10.20961/jthp.v0i0.13010

Abstract

Tujuan penelitian ini adalah mempelajari pengaruh perlakuan pendahuluan terhadap sifat kimia (abu, lemak, protein, asam fitat, dan asam sianida) serta sifat fisik (derajat putih, densitas kamba, dan kelarutan) tepung kacang turi berbunga putih. Penelitian ini menggunakan rancangan acak lengkap dengan satu faktor,yaitu perlakuan pendahuluan yang meliputi: kontrol (F1), perebusan 90 menit dengan kulit (F2), perendaman 24 jam dengan kulit (F3), pengupasan kulit (F4), perebusan 90 menit kupas kulit (F5) dan perendaman 24 jam kupas kulit (F6). Hasil penelitian menunjukkan bahwa seluruh perlakuan pendahuluan menghasilkan tepung dengan kadar abu, asam fitat dan asam sianida (HCN) yang lebih rendah dibandingkan kontrol. Perlakuan pendahuluan pengupasan baik dengan maupun tanpa perebusan 90 menit dan perendaman 24 jam (F4, F5, F6) menghasilkan tepung dengan kadar lemak dan kadar protein lebih tinggi dibandingkan kontrol. Perebusan 90 menit dan perendaman 24 jam menghasilkan tepung dengan kadar lemak dan protein lebih rendah dibandingkan kotrol baik yang dikupas maupun yang tidak dikupas kulitnya (F2, F3, F5, F6). Tepung dengan kadar lemak danprotein tertinggi yaitu tepung dengan perlakuan pendahuluan pengupasan kulit (F4) dan tepung dengan kadar lemak dan protein terendah yaitu tepung dengan perlakuan pendahuluan perendaman 24 jam tanpa pengupasan kulit (F2). Penurunan kadar asam fitat dan asam sianida terbesar terjadi pada perlakuan pendahuluan perebusan 90 menit disertai pengupasan kulit (F5) sedangkan yang terkecil pada perlakuan pendahuluan pengupasan kulit (F4). Secara umum, perlakuan pendahuluan menurunkan densitas kamba dan kelarutan tepung yang dihasilkan, kecuali tepung dengan perlakuan pendahuluan 24 jam tanpa pengupasan kulit (F2) yang memiliki densitas kamba yang tidak berbeda nyata dengan kontrol. Derajat putih tepung dengan berbagai perlakuan pendahuluancukup variatif, yang paling tinggi derajat putihnya adalah tepung dengan perlakuan pendahuluan pengupasan kulit (F4) dan yang paling rendah adalah tepung dengan perlakuan pendahuluan perendaman 24 jam tanpa pengupasan kulit (F2). Kata kunci : pengupasan kulit, perebusan, perendaman, sifat kimia, tepung kacang turi berbunga putih