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All Journal Dentika Dental Journal Jurnal Teknosains MKGK (Majalah Kedokteran Gigi Klinik) (Clinical Dental Journal) UGM Majalah Kedokteran Gigi Indonesia ODONTO Dental Journal JURNAL KESEHATAN LINGKUNGAN (Journal of Environmental Health) Padjadjaran Journal of Dentistry Jurnal Kesehatan Vokasional Jurnal Perikanan Universitas Gadjah Mada Odonto dental journal
Heribertus Dedy Kusuma Yulianto
Departemen Biomedika Kedokteran Gigi, Fakultas Kedokteran Gigi, Universitas Gadjah Mada, Yogyakarta
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Education Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Mechanical Engineering Medicine & Pharmacology Nursing Public Health

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The concentration effect of kulim leaf (scorodocarpus borneensis) extract on Streptococcus mutans ATCC 25175 bacterial hydrophobicity and adhesion Utami, Trianna Wahyu; Hapsari, Adhaninggar Ratna; Hanalda, Dhe Rifdania; Nur, Asikin; Yulianto, Heribertus Dedy Kusuma; Purwanti, Nunuk
Majalah Kedokteran Gigi Indonesia Vol 8, No 3 (2022): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.80636

Abstract

With the ability to reduce hydrophobicity and inhibit the adhesion of S. mutans ATCC 25175 bacteria, Kulim leaf extract can be used as an alternative to chlorhexidine mouthwash in caries prevention. The objectives of this study are to determine the effect of Kulim leaf extract on hydrophobicity and adherence of the cariogenic bacteria S. mutans ATCC 25175. The test groups were divided into negative control (DMSO 2%), the treatment group (Kulim leaf extractconcentrations of 1.25%, 2.5%, 5%), and positive control (0.1% chlorhexidine). All of groups were received three replicated tests for hydrophobicity and adherence inhibition of S. mutans ATCC 25175 bacteria. The hydrophobicity test was conducted by providing 3 ml of bacterial suspension of S. mutans ATCC 25175 which had been adjusted to the McFarland 0.5 standard for each group. Furthermore, each test group was vortexed for one minute and left to stand for 15 minutes. Each treatment was tested with a wavelength spectrophotometer of 550 nm before and after the provision of 200 µl of n-hexadecane. The absorbance value on the spectrophotometer was then included in the hydrophobicity formula to determine the hydrophobicity percentage of S. mutans ATCC 25175 against n-hexadecane. To test the bacterial adhesion, the 96 wells microplate was inserted with the kulim leaf extract of each concentration,BHI-B, bacteria according to the McFarland 0.5 standard, and for the positive control and negative control. Afterwards, they were incubated at 37 °C for 24 hours before they were rinsed with distilled water, and stained with 0.1% crystal violet. Then, an optical density reading was performed using a microplate reader with a wavelength of 540 nm. The absorbance value was then included in the formula for percentage of bacterial adhesion inhibition. Post-Hoc LSD testshowed a significant difference in mean difference between the negative control group and the other treatment groups (p<0.05). In addition, it was revealed that there was no significant mean difference between treatment groups, and there was no significant difference between positive control and treatment groups of 2.5% and 5% in the hydrophobicity test. However, there was a significant difference between the positive control and the treatment group of 1.25% in the hydrophobicity test and the treatment group of 1.25%, 2.5%, and 5% in the adherence test. This study concluded that Kulim leaf extract concentration affected hydrophobicity and attachment of S. mutans ATCC 25175 with an effective concentration of 2.5%.
Antibacterial effect of alpha-mangostin from Garcinia mangostana. L against oral streptococci and staphylococci biofilms: a systematic review Praptiningsih, Rahmawati Sri; Siswomihardjo, Widowati; Jonarta, Alma Linggar; Yulianto, Heribertus Dedy Kusuma; Syifa, Liftia Layyinatus; Rochmah, Yayun Siti; Ichwan, Solachuddin Jauhari Arief
Padjadjaran Journal of Dentistry Vol 36, No 2 (2024): July 2024
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol36no2.53957

Abstract

ABSTRACTIntroduction: Interactions among competing bacteria, which inhibit each other's growth to maintain the balance of microorganisms in the biofilm, can influence oral cavity homeostasis. Natural products contain compounds with the potential to combat various diseases, including caries. Alpha-mangostin from Garcinia mangostana. L exhibits antibacterial properties against oral streptococci and staphylococci, acting through several mechanisms, including the disruption of peptidoglycan, which ultimately leads to cell brittleness and rupture. This study aims to assess the impact of antibacterial alpha-mangostin on oral streptococci and staphylococci biofilm. Methods: The research methodology employed secondary data collection through a systematic review study. We conducted searches across databases including PubMed, ScienceDirect, and Google Scholar to identify Indonesian and English references encompassing textbooks, research findings, reviews, and internet articles relevant to the topic published between 2014 and 2024. Following a thorough screening process, we selected articles deemed pertinent for inclusion in the review. Subsequently, these articles underwent evaluation for full-text accessibility, language compatibility, and availability of information from the respective journals, resulting in the selection of 20 articles. Results: Analysis was carried out on 20 articles. Ten articles described the effects of alpha-mangostin's inhibit oral biofilm, 6 articles discussed the effects of alpha-mangostin's antibacterial activity against Streptococcus mutans and Streptococcus sanguis, 3 article state mechanism alpha-mangostin inhibit membrane enzymes such as F(H+)-ATPase and the phosphoenolpyruvate-sucrose phosphotransferase system on Staphylococcus aureus. Additionally, one article described inhibition mechanisms of aldolase, glyceraldehyde-3-phosphate dehydrogenase, and lactic dehydrogenase on Staphylococcus epidermidis. Conclusion: Alpha-mangostin inhibits and disrupts biofilm defense mechanisms. It possesses antimicrobial properties effective against oral streptococci and staphylococci, including Streptococcus mutans, Streptococcus sanguis, Staphylococcus aureus and Staphylococcus epidermidis. These properties operate through various mechanisms, including enhancing the permeability of bacterial cell walls, ultimately leading to bacterial cell death.KEYWORDSOral biofilm, alpha-mangostin, oral streptococci, staphylococci
Comparison of Bacterial Contamination Before and After Sterilization with UV, Fogging, and Drymist in University Outpatient Dental Hospital Andriyanto, Arif; Solikhah, Solikhah; Suryani, Dyah; Dedy Kusuma Yulianto, Heribertus
JURNAL KESEHATAN LINGKUNGAN Vol. 17 No. 2 (2025): JURNAL KESEHATAN LINGKUNGAN
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jkl.v17i2.2025.187-200

Abstract

Introduction: Microbial contamination in healthcare facilities, particularly in outpatient rooms, raises the risk of nosocomial infections and endangers the health of both patients and personnel. The purpose of this study is to compare the efficacy of three sterilizing methods—ultraviolet (UV) light, fogging, and drymist—in lowering bacterial counts and microbial species in university outpatient dental hospital. Methods: This study uses a quasi-experimental design with a one-group pretest-posttest procedure. This design involves assessing outcomes before and after sterilization in the same group to evaluate the intervention’s effects. Samples were collected before and after the sterilization process. Sterile swabs were used to gather microbial samples from surfaces such as examination tables, walls, and medical equipment within the outpatient area. The data were analyzed using paired t-tests and ANOVA. Results and Discussion: All three sterilizing methods were effective in lowering microbial counts, with the UV approach showing the greatest decrease (83.9%), followed by drymist (79.6%) and fogging (63.4%). However, ANOVA findings revealed no significant difference in effectiveness across the three techniques (p = 0.979). Nonetheless, certain bacterial species that are more resistant to sterilization survived after treatment. Conclusion: UV, fogging, and dry mist sterilizing technologies reduce microbial counts in comparable ways, although their performance may be impacted by ambient conditions and the types of bacteria presents. A mix of sterilizing procedures may be required for best results.
RONGGA MULUT SEBAGAI RESERVOIR POTENSIAL UNTUK INFEKSI Pseudomonas aeruginosa Hajardhini, Puspita; Susilowati, Heni; Yulianto, Heribertus Dedy Kusuma
Odonto : Dental Journal Vol 7, No 2 (2020): December 2020
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (826.693 KB) | DOI: 10.30659/odj.7.2.125-133

Abstract

Introduction : Pseudomonas aeruginosa (P. aeruginosa) is Gram-negative bacteria which is normally inhabiting in environment, however it tends to be an opportunistic pathogen within oral cavity. It utilizes the oral cavity as potential reservoir to infect either lungs or oral cavity itself. Both planktonic and biofilm forms can mediate its infection in oral cavity so that making its difficulties to eradicate since its broad resistance to antibiotics.Method : We review the pathogenesis of oral infection and host defense mechanism to P. aeruginosa as well.Discussion: Several prevention strategies, both chemical and nonchemical, are elaborated to avoid oral bacterial contamination.Conclusion : Prevention strategies in dental aspect to P. aeruginosa is highly needed as its occurrence are susceptible to cross-infection.
Surface roughness and topography on denture base material fabricated with additive manufacturing techniques Abizar Agung Wibawa; Budi Arifvianto; Rini Dharmastiti; Heribertus Dedy Kusuma Yulianto; Dibyo Pramono
Jurnal Teknosains Vol 15, No 2 (2026): June
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/teknosains.116126

Abstract

Denture fabrication plays an important role in restoring oral functions, including mastication, speech, and esthetics, while also helping to prevent temporomandibular joint disorders. Conventional denture bases are commonly fabricated using heat-cured acrylic resin; however, this method still presents several limitations, including longer fabrication time, lower accuracy, and outcomes that are highly dependent on operator performance. The development of additive manufacturing technology offers several advantages, such as high reproducibility, improved accuracy in producing complex geometries, a more efficient digital workflow, and reduced material waste. This study investigated the effect of additive manufacturing using digital light processing (DLP) on the surface roughness and topography of denture bases fabricated with different printing layer thicknesses (50 μm and 100 μm), compared with conventional heat-cured acrylic resin bases. Disc-shaped samples with a diameter of 5 mm and a thickness of 2 mm were analyzed for surface roughness using a profilometer along a 2 mm straight line, while surface topography was observed using scanning electron microscopy (SEM). The results showed significant differences in surface roughness among the groups, where the additive manufacturing group with a 50 μm layer thickness exhibited the smoothest surface (0.96±0.26 μm), followed by the conventional heat-cured group (1.75±0.31 μm), while the additive manufacturing group with a 100 μm layer thickness showed the highest roughness value (4.27±0.55 μm). SEM analysis also revealed crater-like surface defects correlated with variations in layer thickness. These defects were observed more frequently in the additive manufacturing group with a 100 μm layer thickness compared with the 50 μm layer thickness group. These findings confirm the importance of additive manufacturing parameters in determining the final properties of denture bases and indicate the potential for optimizing fabrication techniques to achieve improved denture outcomes.
Co-Authors Abizar Agung Wibawa Agustinus Winarno Alma Linggar Jonarta Alma Linggar Jonarta Andina Widyastuti Andriyanto, Arif Anne Handrini Dewi Arif Andriyanto Asikin Nur Benidiktus Tulung Prayoga Budi Arifvianto Deaoxi Renaschantika Djatumurti Dian Permata Sari Dibyo Pramono Dyah Listyarifah Dyah Suryani Enggardipta, Raras Ajeng Hajardhini, Puspita Hanalda, Dhe Rifdania Haniastuti, Tetiana Hapsari, Adhaninggar Ratna Hendri Susanto Heni Susilowati Heni Susilowati Ichwan, Solachuddin Jauhari Arief Intan Ruspita Intan Ruspita, Intan Julita Hendrartini Liftia Layyinatus Syifa M. Eric Brilian Margareta Rinastiti Margareta Rinastiti Morita Novia Sari Sulistyo Nunuk Purwanti Pribadi Santosa Puspita Hajardhini Rahmawati Sri Praptiningsih Regina Titi Christinawati Tandelilin Rini Dharmastiti Risma Dwi Irfanto Roissatun Nasikah Solikhah Solikhah Trianna Wahyu Utami Widowati Siswomihardjo Yayun Siti Rochmah Zipora Silka Yoretina