Article Per Year (5 Year)
p-Index From 2020 - 2025
2.737
P-Index
This Author published in this journals
All Journal VALENSI Jurnal Penelitian Saintek SAINS DAN MATEMATIKA JURNAL KIMIA SAINS DAN APLIKASI Alchemy Jurnal Penelitian Kimia INDONESIAN JOURNAL OF PHARMACY Indonesian Journal of Chemistry Mendapo: Journal of Administrative Law Current Research on Biosciences and Biotechnology Jurnal Info Sains : Informatika dan Sains Greensphere: Journal of Environmental Chemistry
Ismiyarto Ismitarto
Department Of Chemistry, Faculty Of Science And Mathematics, Diponegoro University Jl. Prof. H. Soedarto SH Tembalang, Semarang 50275, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Energy Engineering Environmental Science Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology

Published : 47 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 47 Documents
Search

 1   2   3   4   5 
Penentuan total fenolik dan flavonoid serta uji aktivitas antioksidan dari daun mangga kultivar madu Fachriyah, Enny; Wati, Mei Riska; ismiyarto, Ismiyarto; Sarjono, Purbowatiningrum Ria
Jurnal Penelitian Saintek Vol 29, No 2 (2024)
Publisher : Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jps.v1i2.77028

Abstract

Penelitian ini bertujuan untuk melakukan uji fitokimia yang meliputi uji alkaloid, flavonoid, saponin, fenolik, kuinon, triterpenoid dan steroid, total fenolik dan flavonoid serta aktivitas antioksidan fraksi etil asetat dan etanol daun mangga kultivar madu. Penelitian diawali determinasi tanaman, preparasi sampel, skrining fitokimia, maserasi dengan etanol 96% dilanjutkan partisi bertingkat menggunakan n-heksana dan etil asetat. Skrining fitokimia dilakukan pada ekstrak dan fraksi yang diperoleh. Fraksi yang diperoleh ditentukan total fenolik dengan metode Folin-Ciocalteu, total fl avonoid dengan metode kolorimetri AlCl3. Aktivitas antioksidan ditentukan dengan metode DPPH. Hasil determinasi tanaman dapat dipastikan sampel adalah Mangifera indica L. kultivar madu, positif mengandung senyawa flavonoid, fenolik, saponin, kuinon, steroid dan triterpenoid. Rendemen fraksi etil asetat dan fraksi etanol berturut-turut sebesar 6,19 dan 5,19%. Kadar total fenolik fraksi etil asetat daun mangga kultivar madu berturut-turut sebesar 423,7 ± 3,68 mg GAE/g dan 48,2 ± 0,139 mg QE/g. Kadar total flavonoid fraksi etanol berturut-turut sebesar 132,35 ± 1,95 mg GAE/g dan 27,72 mg QE/g. Nilai IC50 fraksi etil asetat dan fraksi etanol berturut-turut sebesar 17,83 dan 79,73 ppm. Daun mangga kultivar madu dapat berperan sebagai agen antioksidan dengan kategori kuat pada fraksi etanol dan sangat kuat pada fraksi etil asetat.Kata kunci: total fenolik, total flavonoid, antioksidan, Mangifera indica LDetermination of total phenolics and flavonoids content and test of antioxidant activity of mango leaves honey cultivar Abstract: This research aims to carry out phytochemical tests which include tests for alkaloids, flavonoids, saponins, phenolics, quinones, saponins, triterpenoids and steroids, total phenolics and fl avonoids as well as antioxidant activity of the ethyl acetate and ethanol fractions of honey cultivar mango leaves. The research began with plant determination, sample preparation, phytochemical screening, maceration with 96% ethanol followed by multilevel partitioning using n-hexane and ethyl acetate. Phytochemical screening was carried out on the extracts and fractions obtained. The fractions obtained were determined as total phenolics using the Folin Ciocalteu method, total flavonoids using the AlCl3 colorimetric method. Antioxidant activity was determined by the DPPH method. The results of the plant determination confi rmed that the sample was Mangifera indica L. honey cultivar, positive for containing flavonoid, phenolic, saponin, quinone, steroid and triterpenoid compounds. The yields of the ethyl acetate fraction and ethanol fraction were 6.19 and 5.19% respectively. The total phenolic content of the ethyl acetate fraction of honey cultivar mango leaves was respectively 423.7 ± 3.68 mg GAE/g and 48.2 ± 0.139 mg QE/g. The total flavonoid content of the ethanol fraction was 132.35 ± 1.95 mg GAE/g and 27.72 mg QE/g, respectively. The IC50 values of the ethyl acetate fraction and ethanol fraction were 17.83 and 79.73 ppm respectively. Honey cultivar mango leaves can act as an antioxidant agent with a strong category in the ethanol fraction and very strong in the ethyl acetate fraction. Keywords: total phenolics content, total flavonoids content, antioxidant, Mangifera indica L
Synthesis of Antibacterial Coating Film Based on Eugenol-Allyl Eugenol Copolymer with Chitosan-Gelatin Ngadiwiyana, Ngadiwiyana; Dzahabiyyah, Hana Putri; Ismiyarto, Ismiyarto; Sarjono, Purbowatiningrum Ria
Jurnal Kimia Valensi Jurnal Kimia VALENSI, Volume 10, No. 2, November 2024
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v10i2.40944

Abstract

The development of coating film materials based on biopolymers and active antibacterial compounds has attracted attention in the food industries. Food packaging biopolymers can be increased antibacterial properties by adding compound modification of natural ingredients such as eugenol-allyl eugenol copolymer (PEAE). The aims of this study were to synthesize a coating film based on chitosan-gelatin with PEAE and test its antibacterial properties. PEAE synthesis was carried out by polymelirization reaction with the (BF3O(C2H5)2) as catalyst and characterized by FTIR, molecular weight, and solubility. Synthesis of chitosan-gelatin coating films with variations in PEAE concentration of 1.25%, 2.5%, and 3.75% and characterization includes FTIR, SEM, TS, E%, and contact angle. Antibacterial activity is carried out by the turbidimetry method. PEAE was synthesized with the results in the form of brown solids with 94.91% yield, molecular weight of 9,553.98 da, and the melting point of 95-98 °C. Chitosan-Gelatin films with the addition of PEAE produce a thin yellowish film, with a sequential decreased tensile strength, and the percentage value of extension increases with the increase in PEAE concentration. The best antibacterial activity in the film PEAE 2.5, with the percentage of inhibition of Staphylococcus aureus and Escherichia coli of 99.71% and 98.39% respectively.
IMPLEMENTASI REFORMASI BIROKRASI DAN PELAYANAN INVESTASI PADA DINAS PENANAMAN MODAL DAN PELAYANAN TERPADU SATU PINTU PROVINSI JAWA TENGAH INDONESIA: Implementasi Reformasi Birokrasi dan Pelayanan Investasi Pada Dinas Penanaman Modal dan Pelayanan Terpadu Satu Pintu Enceng, Enceng; Purwaningdyah, Purwaningdyah; Ismiyarto, Ismiyarto
Mendapo: Journal of Administrative Law Vol. 4 No. 3 (2023): Oktober 2023
Publisher : Fakultas Hukum Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/mendapo.v4i3.29290

Abstract

Public service from upstream to downstream is filled with friendship, ethnicity, and political affiliation. It is common knowledge that the public service process, including investment services, is greatly influenced by who gets what in which relationship. The public service process does not have transparency in terms of time, costs, and procedures that must be followed, resulting in complicated procedures and quite high costs. Licensing procedures involving central authority are difficult to issue quickly in a simple process. Regulatory uncertainty is part of bad governance, this challenge must be overcome so that investors are attracted to Indonesia. Investors are not interested in entering Indonesia but have difficulty realizing their investment. The problem is not as easy as turning the palm of the hand to make the investment climate in Indonesia attract investors. Issues related to ease of licensing, overlapping laws and regulations, and regulations that are out of sync are still often heard. The concept of one-stop integrated services to electronically integrated business licensing has been echoed. For this reason, reforming business licensing and regulations is a national priority agenda, the government is committed to providing legal certainty for investors and business actors. This is one method of bureaucratic reform, namely "regulation and deregulation" meaning that for things that have not been regulated, regulations need to be carried out. In contrast, for existing laws and regulations that are considered to cause various problems, deregulation needs to be carried out. This research study method uses a qualitative descriptive approach. Generally, studies of the symptoms, processes and meaning of regulation are more directed at economic regulations which have implications for the economic sector or regulations for economic activities or sectors in a country, especially markets.
Antioxidant and antibacterial activities of alkaloids from endophytic bacterium Pseudomonas hibiscicola Wk isolated from waru (Hibiscus tiliaceus) leaves Sarjono, Purbowatiningrum Ria; Laksmitasari, Nuraini Dwi; Asy'ari, Mukhammad; Ngadiwiyana, Ngadiwiyana; Ismiyarto, Ismiyarto; Fachriyah, Enny
Jurnal Penelitian Saintek Vol 30, No 1 (2025)
Publisher : Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jps.v30i1.77501

Abstract

This study aims to investigate the antioxidant and antibacterial activity from alkaloid extract. Alkaloid compounds were isolated from the endophytic bacteria Pseudomonas hibiscicola Wk, which was obtained from Hibiscus leaves. The alkaloid compounds were characterized using thin layer chromatography. The isolated alkaloid compounds were tested for total phenol content, and antioxidant activity. The phytochemical analysis of the alkaloid compounds from the endophytic bacteria Pseudomonas hibiscicola Wk showed a positive result for alkaloids, confirmed by an Rf value of 0.8125 which is similar to the Rf value of alkaloids from the periwinkle plant, namely vindoline (Rf = 0.85). Alkaloid compounds from the endophytic bacteria Pseudomonas hibiscicola Wk had the highest total phenol content of 2.390 mg gallic acid/gram sample at a concentration of 10.000 mg/L. These results indicate that the alkaloid compounds obtained contain phenol groups. The IC50 value obtained from the isolated alkaloid compound was 99.70 mg/L, demonstrating strong antioxidant capacity, and has the ability to inhibit the growth of E.coli and S.aureus bacterial.
Total Phenolic Content Analysis, Antioxidant Activity, and Isolation of Phenolic Acid Compounds from Methanol Extract of Celery (Apium graveolens L.) Hanum, Rahma Farida; Fachriyah, Enny; Ismiyarto, Ismiyarto; Sarjono, Purbowatiningrum Ria
Jurnal Kimia Sains dan Aplikasi Vol 28, No 7 (2025): Volume 28 Issue 7 Year 2025
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jksa.28.7.345-354

Abstract

Celery (Apium graveolens L.), a member of the Apiaceae family, is rich in bioactive compounds and exhibits various health benefits, including antihypertensive, antibacterial, anti-inflammatory, and antioxidant activities. The presence of diverse phenolic acids, as identified in previous studies, prompted this research to analyze the total phenolic content, evaluate antioxidant activity, and isolate phenolic acids from the methanol extract of celery. The study involved phytochemical screening, maceration extraction, determination of total phenolics using the Folin–Ciocalteu method, antioxidant testing via the DPPH method, and phenolic acid isolation using three approaches: base hydrolysis (HB), acid hydrolysis (HA), and non-hydrolysis (TH). The isolates were analyzed using UV–Vis spectrophotometry, CMS, and LC/MS-MS QTOF. The methanol extract of celery yielded 16.59% and was found to contain flavonoids, phenolic acids, saponins, tannins, and steroids. The total phenolic content was 17.268 ± 0.057 mg GAE/g extract, while the IC50 value was 1712.782 ± 0.115 mg/L, indicating very weak antioxidant activity. Isolation of phenolic acids revealed that the HA fraction contained pyrogallol and caffeic acid. This was supported by the A3 band (λmax 230 nm and 269 nm) with a molecular weight of 126 m/z, characteristic of pyrogallol. The A4 band (λmax 218, 242, 298, and 325 nm) with a molecular weight of 180.0387 m/z was characteristic of caffeic acid.
Isolation of Endophytic Pseudomonas Strains from Papaya Leaves and Their Extracellular Enzyme Production and Antioxidant Profile Sarjono, Purbowatiningrum Ria; Choirunnisa, Nur Fadilla; Triwijayanti, Yunita; Salsabila, Salsabila; Asy’ari, Mukhammad; Ngadiwiyana, Ngadiwiyana; Ismiyarto, Ismiyarto; Prasetya, Nor Basid Adiwibowo; Andriani, Yosie
Jurnal Kimia Valensi Vol. 11 No. 1 (2025): Jurnal Kimia VALENSI
Publisher : Department of Chemistry, Faculty of Science and Technology Syarif Hidayatullah Jakarta State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v11i1.40921

Abstract

Endophytic bacteria, symbiotic microorganisms residing in plant tissues, produce bioactive compounds similar to host plants, such as antioxidants. These antioxidants are crucial in combating free radicals linked to degenerative diseases. This study isolates and characterizes two endophytic bacterial strains from papaya leaves, exploring their enzymatic and antioxidant activities. Two isolates of endophytic bacteria from papaya leaves were obtained, F1-A and F1-B. F1-A endophytic bacteria are types of monobacilli, Gram-positive bacteria. F1-B endophytic bacteria are types of Bacilli. Using 16S rRNA analysis, both isolates were predicted to belong to the Pseudomonas bacterial strain. Research on optimizing their growth under various temperatures and pH conditions showed that both isolates grow best at 37°C. F1-B provides a better opportunity as a source of industrial enzymes because it can excrete amylase, urease, cellulose, and protease enzymes compared to F1-A, which can only produce amylase and protease enzymes. Nevertheless, F1-A can act as a potent antioxidant with an IC50 of 34.18 ppm compared to F1-B, which has an IC50 value of 292.31 ppm. The IC50 value of the F1-A isolate was not much different from the IC50 of quercetin, which was 12.50 ppm. The ability of F1-A as an antioxidant is also influenced by the results of phytochemical screening, which can contain more secondary metabolites than F1-B. These results highlight the potential of Pseudomonas strains as sources of industrial enzymes and natural antioxidants, warranting further investigation.
Synthesis and Antibacterial Activity of Chitosan-Cinamaldehyde/AgNp Schiff Base Composite Huda, Muhammad Badrul; Rinaryadi, Kemilau Permata Hati; Ngadiwiyana, Ngadiwiyana; Christwardana, Marcelinus; Sarjono, Purbowatiningrum Ria; Ismiyarto, Ismiyarto
Jurnal Kimia Valensi Vol. 11 No. 1 (2025): Jurnal Kimia VALENSI
Publisher : Department of Chemistry, Faculty of Science and Technology Syarif Hidayatullah Jakarta State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v11i1.45121

Abstract

Chitosan is a material that has antibacterial properties. Chitosan was modified with cinnamaldehyde to form chitosan Schiff base, which acts as a capping agent in the synthesis of silver nanoparticles. The Schiff base product was modified again into a silver nanoparticle Schiff base composite to improve its ability as a capping agent and improve its antibacterial properties. This study aims to synthesize a chitosan-cinnamaldehyde/AgNP Schiff base composite (CCSB/AgNP) as an active ingredient with excellent antibacterial properties. The first stage was the synthesis of a chitosan-cinnamaldehyde Schiff base. In the second stage, the synthesis of the chitosan/AgNP composite was carried out by adding STPP with sonication and a water bath. The third stage involved synthesizing of the CCSB/AgNP composite using the same method as the second stage employing both heating and non-heating as well as sonication and non-sonication. The product was characterized using a UV-Vis spectrophotometer, FT-IR, SEM-EDX, mapping, and AAS. Antibacterial tests were performed on the synthesized product using the Total Plate Count (TPC) method. Chitosan has a molecular weight of 338080 g/mol and a degree of deacetylation of 65.09%. The Schiff base product of chitosan-cinnamaldehyde is a brownish-yellow solid with a yield of 76.9% (w/w) and a degree of substitution of 87.02%. The presence of Ag was confirmed by EDX mapping, which revealed mass percentages of 0.26%, 1.00%, and 3.97% for C/AgNP-1, C/AgNP-2, and CCSB/AgNP-2, respectively. The chitosan/AgNP product has a yield of 97% (w/w) and an SPR effect at 439 nm. The synthesis of CCSB/AgNP obtained a dark green solid with a yield of 87% (w/w) and an SPR effect at 433 nm. The antibacterial activity test yielded the highest percentage reduction in the number of bacteria in CCSB/AgNP at 3 days of observation at 95.1%, and 7 days at 94.1%. 
Co-Authors A.A. Ketut Agung Cahyawan W Adityo Anggadita Agus Subagio Ahmad Fathoni Alik Kandhita Febriani Anjar Jatika Anshori, Yuli Tirtariandi El Ardhita Niken Destiana Asy'ari, Mukhammad Asy’ari, Mukhammad Ayu Ratri Kartika Iriany Bambang Cahyono Bayu Refindra Fitriadi Chairil Anwar Chairil Anwar Choirunnisa, Nur Fadilla Damar Nurwahyu Bima Desy Fitrya Syamsumir Didik Setiyo Widodo Dimas Syamsudin Ditya Putri Solihati Dzahabiyyah, Hana Putri Enceng Enceng Enny Fachriyah Enny Fachriyah Fannie Kurniasari Fithri Noorikhlas Halim, Siti Anisah Hanum, Rahma Farida Hendra Dwipa Rifky Mahardika Hermansyah Amir Huda, Muhammad Badrul Intan Endah Rachmawati Jumina Jumina Khairul Anami Laksmitasari, Nuraini Dwi Leong Kai Vyn Liswinda Zafirah Rahmatia Madiyono, Madiyono Marcelinus Christwardana Meiny Suzery Mumtazati, Qonita Murni Nur Islamiah Kassim Ngadiwiyana M.Si. S.Si. Nies S. Mulyani Niken Windi Saputri Ningsih, Alyxia Stellata Aria Noor Basid Adiwibawa Prasetya Nor Basid Adiwibawa Prasetya Nor Basid Adiwibawa Prasetya Nur Amaliyah Nurlina Priastuti Pandelaki, Elmi Christi Julia Pratama Jujur Wibawa Purbowatiningrum Ria Sarjono Purwaningdyah, Purwaningdyah Rani Mustika Rinaryadi, Kemilau Permata Hati Sabirin Matsjeh Salsabila, Salsabila Sesika Novari Suradi, Agus Suyanti Suyanti Triwijayanti, Yunita Wati, Mei Riska Yosie Andriani Yosie Andriani