Claim Missing Document
Check
Articles

Found 2 Documents
Search
Journal : Jurnal Veteriner

Isoprostan Urin Sebagai Biomarka Keracunan Etanol dan Upaya Detoksikasinya dengan Ekstrak Etanol Kulit Buah Manggis (URINE ISOPROSTANE AS TOXIC BIOMARKER OF ETHANOL AND DETOXICATION EFFORTS BY USING ETHANOL EXTRACT OF SKIN MANGOSTEEN) Ni Made Suaniti; Manuntun Manurung
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (90.427 KB)

Abstract

Isoprostane as a marker of oxidative stress which is a toxic metabolite of fatty acids, can be formedafter experiencing lipid peroxidation duo to ethanol. The aim of this study was to analyze the isoprostaneas early detection biomarker in urine of Wistar rats after ethanol consumption in sub-acute, followed byadministration of ethanol extract of rind of mangosteen fruit (EERMF). ELISA was use in this study fordiagnostic method as it can analyze the content of biological fluids even in small amount. Detection ofisoprostane compounds in the urine Wistar rats after ingestion of ethanol 20%v/v in sub acute was (0.8837± 0.0625) ng/mL decrease levels (0.4500 ± 0.0625) ng/ mL after administration EERMF 20% w/v. Isoprostancan be used as biomarker ethanol toxic and decreased level after giving EERMF.
Kerusakan Hati Akibat Keracunan Alkohol Berulang pada Tikus Wistar (LIVER DAMAGE DUE TO ALCOHOL INTOXICATION REPEAT IN WISTAR RATS) Ni Made Suaniti; Anak Agung Gede Sudewa Djelantik; Ketut Suastika; Nyoman Mantik Astawa
Jurnal Veteriner Vol 13 No 2 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (139.083 KB)

Abstract

The aims of this study was to determine the liver damage from alcohol intoxication in Wistar rats.The design used in this study was a randomized true experimental post test only control group design. Thestudy used 15 rats divided into 3 treatment groups each of which consists of 5 rats. The first group wasgiven distill water. The second group was given 5% alcohol, and the third group was given 20% alcohol. Ratswere treated with alcohol daily for six weeks. Biochemical markers were detected the levels of aldehydedehydrogenase (ALDH) in serum and histological changes in liver tissue. ALDH is a biochemical markerof a sensitive and specific ethanol after chronic alcohol administration. Blood sample was collected at 6and 24 hours after the last peroral administration of repeated alcohol treatment, and serum levels ofALDH was tested by enzyme linked immunosorbent assay (ELISA). The results showed that the levels ofALDH in the blood of alcohol treated Wistar rats significantly higher as compared to those of control rats.ALDH levels increased by 83.11% after administration of 5% alcohol and 112.05% after administration of20% taken after 6 hours of alcohol for 6 weeks. On samples taken after 24 hours, ALDH levels by 95.11%after administration of 5% alcohol and 86.79% after administration of 20% alcohol. Oral treatment with20% alcohol chronically was led to changes in the microscopic structure (necrosis) of liver tissue in Wistarrats. Liver tissue damage occured due to repeated use of alcohol is accompanied by increasing serum levelsof ALDH in Wistar rats.
Co-Authors A. A. B. Putra A. A. Bawa Putra A. A. I. S. J. Dewi A. A. S. D. Saraswati Abdul Rahim Agung Ari Chandra Wibawa Anak Agung Gede Sudewa Djelantik Anak Agung Istri Agung Mayun Laksmiwati Anak Agung Sagung Desy Dwi Martayani Martayani Ariati, N. K. Ayu Kasmita Dewi Darma Yanti, N. M. D. Dewa Ayu Ika Pramitha Dewi Yuliana Dita Rizkiyanti Dwi Anggraeni Putri Suandi H. Purnomo Hendra Wijaksana I D. G. Putra Prabawa I G. Mustika I G.N.O. Suputra I Gede Budiartawan I GEDE PUTU WIRAWAN I Gusti Ayu Kunti Sri Panca Dewi I Gusti Komang Dwijana I Ketut Budiarta I Ketut Suastika I Ketut Suastika I Made Agus Gelgel Wirasuta I Made Dira Swantara I Made Sudarsana I Nengah Suarnadwipa I Nengah Suweden I Nengah Wirajana I NYOMAN MANTIK ASTAWA I W. Wiratama I Wayan Bandem Adnyana I Wayan Suarsa I. A. R. Astiti Asih I. E. Radiati I. Mahmudi I.P. Widiarta Ida Ayu Okarini Ida Bagus Putra Manuaba Ika Paramitha, Dewa Ayu Iryanti Eka Suprihatin James Sibarani Ketut Gede Dharma Putra Ketut Ratnayani Khairul Mahfuz Komang Ari Gunapria Darmapatni M. M. Manurung M. Manurung M. Sucipta M.Pd S.T. S.Pd. I Gde Wawan Sudatha . Made Ayu Hita Pretiwi Suryadhi Made Suarda Mahardika Aprilia Iflahah Mahardika Aprilia Iflahah Manuntun Manurung Maryana, Himayari Nufus Mirah Surya Dewi, Ni Putu Pande N. G. Indriyaningsih N. P. Widya Astuti N. P. Widya Astuti N. W. Bogoriani Nadya Hartasiwi Ni G. A. M. Dwi Adhi Suastuti Ni Komang Ariati Ni Luh Kasih Ariani Ni Luh Rustini Ni Made Meryana Utari Ni Made Puspawati Ni Made Rai Suarni Ni Made Sukma Sanjiwani Ni Nyoman Nadia Angela Kusuma P. Ni Pt. Pande Mirah Surya Dewi Ni Putu Diantariani Ni Putu Widya Astuti Ni Wayan Sri Sukmarianti Noviyanti, Ni Putu O. Ratnayani O. Ratnayani Oka Ratnayani Oka Ratnayani Oktavia, Fadilla Raisyah Anjani Riyadi, T. Rr. Anisa Hernindya S. A. Sari Sagung Ngurah Mayuni Setianingsih, Ni Luh Putu Putri Turmuzi Tammi Wahyu Dwijani Sulihingtyas Wira Adi Capayanti WIWIK SUSANAH RITA