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All Journal INDONESIAN JOURNAL OF LEGAL AND FORENSIC SCIENCES International Journal of Biosciences and Biotechnology METAMORFOSA Journal of Biological Sciences Buletin Udayana Mengabdi Jurnal Biologi Udayana Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) Journal of Health Sciences and Medicine Jurnal Arjuna : Publikasi Ilmu Pendidikan, Bahasa dan Matematika

Inna Narayani

Program Studi Biologi FMIPA Universitas Udayana Bali

Author-ID : 2925891

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Social Sciences

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VARIASI ALEL DNA MIKROSATELIT AUTOSOM LOKUS D2S1338, D13S317 DAN D16S539 PADA MASYARAKAT DAYAK KAHARINGAN DI KOTA PALANGKA RAYA Lucia Emy Octavia; I Ketut Junitha; Inna Narayani
Jurnal Biologi Udayana Vol 19 No 2 (2015): JURNAL BIOLOGI
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (227.139 KB)

Penelitian ini dilakukan untuk mengetahui ragam alel masyarakat Dayak Kaharingan di Kota Palangka Raya. DNA diekstraksi dari sel epitel mukosa mulut, dari 26 individu dengan metode fenol kloroform. DNA mikrosatelitautosom lokus D2S1338, D13S317 dan D16S539 diamplifikasi pada mesin PCR. Pengamatan hasil PCR dilakukan dengan Polyacrylamide Gel Electrophoresis (PAGE) dan visualisasi DNA hasil PCR dengan pewarnaan perak nitrat. Hasil penelitian ini menunjukkan terdapat 29 alel dari ketiga lokus yaitu lokus D2S1338 sebanyak 11 alel, serta masing-masing sembilan alel pada lokus D13S317 dan lokus D16S539. Nilai heterozigositas tertinggi terdapat pada lokus D2S1338 yaitu 0,8971 dengan kekuatan pembeda (PD) 0,9682, diikuti lokus D13S317 dengan kekuatan pembeda 0,9339 dan lokus D16S539 dengan kekuatan pembeda 0,9226.

GENETIC DIVERSITY OF SKIPJACK TUNA (Katsuwonus pelamis) FROM JEMBRANA AND KARANGASEM REGENCIES, BALI Fakhrurrasi Fakhri; Inna Narayani; I G. Ngurah Kade Mahardika
Jurnal Biologi Udayana Vol 19 No 1 (2015): JURNAL BIOLOGI
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Penelitian ini bertujuan untuk mengetahui keragaman genetik ikan cakalang (Katsuwonus pelamis) yang ada di Kabupaten Jembrana dan Karangasem, Bali. Sampel DNA diambil dari 30 ekor di kabupaten Jembrana dan 30 ekor di kabupaten Karangasem. Ekstraksi DNA dilakukan dengan menggunakan chelex 10% dan amplifikasi menggunakan metode PCR dengan konfigurasi HotStart pada control region DNA mitokondria, menggunakan primer forward CRK dan primer reverse CRE. Analisis filogenetika dilakukan dengan menggunakan metode Neighbor Joining dengan model Kimura 2-parameter. Variasi dari setiap sekuen diperoleh dengan menghitung jumlah, persentase dari tiap haplotipe, keragaman haplotipe (hd) dan keragaman nukleotida (?) menggunakan program DnaSP 5.10. Analisis Fst (Fixation Index) berdasarkan frekuensi haplotipe menggunakan Arlequin 3.1. Haplotipe pada sampel Jembrana berjumlah 28 haplotipe dan sampel Karangasem berjumlah 15 haplotipe dengannilai hd secara keseluruhan yaitu 0,997±0,006 dan untuk keragaman nukleotida (?) sebesar 0,04694. Nilai Fst yang diperoleh yaitu -0,00334 dan Fst P sebesar 0,90918±0,0100, menunjukkan bahwa populasi ikan cakalang di Kabupaten Jembrana dan Karangasem berasal dari populasi induk yang sama.

IDENTITAS GENETIK KETURUNAN SOROH PANDE DI KECAMATAN SERIRIT, BULELENG BERDASARKAN DNA MIKROSATELIT KROMOSOM Y I Wayan Arya Mahardika; I Ketut Junitha; Inna Inna Narayani
Indonesian Journal of Legal and Forensic Sciences (IJLFS) Vol 6 (2016): Indonesian Journal of Legal and Forensic Sciences
Publisher : Penerbit, sejak 2012 : Asosiasi Ilmu Forensik Indonesia dan UPT Lab. Forensik Sain dan Kriminilogi - Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJLFS.2016.v06.i01.p01

The Balinese clans embraced patrilineal system.They divided into groups of soroh or clans which means respect for ancestors. Pandeclan is a unique soroh which comes from ancestors who have expertise in memande. The genetic identityof this clan in Bali is unknown.Therefore the aim of this study is to find out the genetic identity from the varieties of allelic diversity which found on Pande community inside and outside Seririt using microsatellite DNA analysis of Y chromosome, namely DYS19, DYS390, DYS393, and DYS395. Resultsof this study have foundthat in Pande clan there are 21 varieties of allelic diversity and 25 haplotypes with the average heterozygosity of four loci is 0.72 ± 0.03. The dendogram indicates that Pande clanfrom district of Seririt did not cluster based on it’s dadia.

The Effect of Exposure of Cigarette Smoke With Herb Additives on Leukocyte and Lung Histopathology of Mice (Mus musculus) M Luthfi Ardiansyah; A.A.S.A Sukmaningsih; Inna Narayani
Jurnal Ilmiah Biosaintropis (Bioscience -Tropic) Vol 7 No 1 (2021): Agustus 2021
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

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Smoking habits have been around since ancient times, but nowadays this habit is considered to be detrimental, especially to health. The impact that is often felt by smokers is difficulty in breathing because the lungs are exposed to cigarette smoke. Cigarette smoke contains about 1015-1017 oxidants or free radicals, as well as 4700 harmful chemicals, including aldehydes / carbonyls, NO2, and SO2. Herbal cigarettes are tobacco cigarettes with added ingredients from plants. Gurah terapi sin cigarettes are herbal cigarettes that are sold commercially. The aim of this study was to determine the effect of gurah cigarette smoking on the leukocytes and lung histology of mice. This study used a comparative method consisting of 3 groups, namely the control was not exposed to cigarette smoke, treatment 1 was exposed to commercial cigarette smoke and treatment 2 was exposed to cigarette smoke with herbal ingredients and each group consisted of 10 replications. The results showed that there were significant differences (p <0.05) regarding the number of cell necrosis, type II pneumocytes, inflammatory cell infiltration, hemorrhage, and alveolar dilation. While the results of the analysis of the number of leukocytes showed no significant difference where p > 0.05. The results showed that there was no significant difference in the number of leukocytes in the control group, treatment 1 and treatment 2 (p > 0.05). herbs containing various kinds of antioxidants cause a tendency for differences in the number of leukocytes where there is a decrease in the number of lymphocytes and neutrophils and an improvement in the histopathological structure of the lung against type I pneumocyte cell necrosis, hemorrhage, alveolar dilation, type II pneumocyte cell proliferation, and inflammatory cell infiltration in exposed mice. commercial cigarette smoke without herbal ingredients.

P-Glycoprotein Expression on Patients with Acute Lymphoblastic Leukemia R. Niiruri; I. Narayani; K. Ariawati; S. Herawati
Journal of Health Sciences and Medicine Vol 1 No 1 (2017): JHSM (Febuary 2017)
Publisher : Institute for Research and Community Services Udayana University

Abstract Objective: P-glycoprotein (P-gp) overexpression on neoplastic cells can deteriorate the therapeutic outcome on cancer patients. P-gp plays important role on drug efficacy and toxicity. This research aimed to measure P-gp expression on children with Acute Lymphoblastic Leukemia (ALL) on Sanglah Hospital, Denpasar. Method: Flowcytometry method was used to measure P-gp expression level on Bone Marrow samples from pediatric patients (0-12 years old) who were newly diagnosed with ALL in Sanglah Hospital. P-gp overexpression were based on the percentage of cell stained. Ten percent of P-gp expression were considered as the cut-off value of P-gp overexpression. Result: On this study, 11 samples were obtained with the range value of 56-97% on P-gp expression. Conclusion: All 11 patients had P-gp overexpression.

PENDAMPINGAN PENGADAAN BAHAN PRAKTUKUM ZOOLOGI DAN BIOTEKNOLOGI BAGI GURU SMA DI KABUPATEN KLUNGKUNG N.I. Wiratmini; A.A.S. Sukmaningsih; I. Narayani; M. Pharmawati
Buletin Udayana Mengabdi Vol 21 No 3 (2022): Buletin Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Advances in molecular biology has brought a new branch of multidiscipline study named biotechnology. To comprehend the complex nature of concepts in biology, practical and hands-on approaches are often required. This activity was a collaboration between Biology Teachers Working Group (MGMP Biologi) in Klungkung Regency with university as the facilitator, and aimed to train senior high school biology teachers in compiling learning modules as well as supplying them with the appropriate materials required in simple practical sessions, such as those in animal physiology and reproduction, as well as biotechnology. The event was held successfully, attended by total of 20 biology teachers from six senior high schools (SMA) in Klungkung District, Klungkung Regency, started by introductory lectures and followed by practical sessions on how to perform mice vaginal and sperm smear, human blood smear, and DNA extraction of plant tissues. Keywords: practicals, zoology, biotechnology, senior high school students

Deteksi Cemaran Bakteri pada Sampel Daging Sapi dengan Uji TPC Ratu Ayu Ningrat; Ida Bagus Gede Darmayasa; Inna Narayani
Jurnal Arjuna : Publikasi Ilmu Pendidikan, Bahasa dan Matematika Vol. 2 No. 5 (2024): Oktober : Jurnal Arjuna : Publikasi Ilmu Pendidikan, Bahasa dan Matematika
Publisher : Asosiasi Riset Ilmu Pendidikan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61132/arjuna.v2i5.1190

Balai Karantina Pertanian Kelas I Denpasar is located on Jl. Raya Sesetan No 312, Denpasar which is a place of seclusion and/or action as an effort to prevent the entry and spread of pests and animal diseases or nuisance organisms from abroad and from one area to another within the country, or exit from territory of the Republic of Indonesia. The people's need for beef is increasing, demanding more production in order to reach many consumers in various regions. This causes beef producers to pay attention to the quality of the meat to be marketed so that the meat becomes safe. The Total Plate Count (TPC) is one of the initial tests to identify the general microbial count in meat. Therefore, this study aims to determine the TPC in samples of beef that will be marketed outside Bali. There were 20 samples of beef, originating from 5 beef companies in Bali that beef outside the island of Bali through testing services from the Class 1 Agricultural Quarantine Center Denpasar. The test results stated that most of the beef samples from the five companies were contaminated with microbes. The feasibility of consuming the beef sample was declared feasible in accoaccord per 2008 SNI standard 1 x 106 CFU/g.

Co-Authors A.A.S. A. Sukmaningsih Adi Santoso Ahmad Hamim Sadewa Alayna Lillahida Indri Himawan Anak Agung Ketut Darmadi Anak Agung Yulia Anggiani Christian Subagya Gunardi Endah Puji Septisetyani Fakhrurrasi Fakhri Galih Anindita Kusumatjahja Haryo Seto Wicaksono I Gusti Ngurah Kade Mahardika I Ketut Junitha I KETUT MUKSIN I Made Sara Wijana I NYOMAN MANTIK ASTAWA I Putu Agus Tirta Cahyana I Wayan Arya Mahardika Ida Bagus Gede Darmayasa IRIANI SETYAWATI Ketut Ariawati Lucia Emy Octavia M Luthfi Ardiansyah MADE PHARMAWATI Meitini Proborini Wahyuni Ngurah Intan Wiratmini Ni Komang Frely Astuti Ni Luh Eka Setiasih Ni Luh Putu Agustini Ni Putu Eka Krisnayanti Nurul Maulida Rahma Pekik Wiji Prasetyaningrum Putu Desy Yustinadewi R. Niiruri Rasmaya Niruri Rasmaya Niruri Ratu Ayu Ningrat Retno Kawuri Revi Juniar Nutrihidayah Rochmalia Juniarti Putri S. Herawati Wayan Tunas Artama YOSSY CAROLINA UNADI Yustiantara, Putu Sanna

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