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Pujiati
Pendidikan Biologi, Fakultas Keguruan Dan Ilmu Pendidikan, Universitas PGRI Madiun
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Journal : Proceeding Biology Education Conference

 1 
Characteristics of Pigeon Pea (Cajanus Cajan) Isoflavones Daidzein in Blood on Ovarian And Mammary Tissue Structure Rat Female Primiani, Cicilia Novi; Pujiati, Pujiati
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 13, No 1 (2016): Prosiding Seminar Nasional XII Biologi
Publisher : Universitas Sebelas Maret

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Abstract

The use of synthetic hormone preparations, one of the hormones estrogen widely used by people in the care and  treatment of diseases as hormone replacement therapy. Pigeon pea (Cajanus cajan) as local Leguminoceae plant has not been used optimally as estrogenic plants . The purpose of this study was to identify preclinical to analyze the Cajanus cajan in the blood profile and potential of the reproductive organs as phytoestrogens. The research method using 24 Sprague -Dawley female rats 6-7 months , grouped 3 treatment. The control group , the treatment group pigeon pea solution of 1 : 1 and group treatment solution pigeon pea 1 : 3. Blood sampling on the 8th, 16th hour and the 24th hour (fractions I, fractions II and fractions III ) for the analysis of isoflavones daidzein HPLC method . Treatment pigeon pea solution made up to 36 days. Surgery and organ harvesting ovarian and mammary continued hematoxylin eosin staining . The results showed levels of isoflavones daidzein maximum in treatment 1 : 1, fractions I, II , and III respectively 92773.474 pg / l ; 96766.278 pg / l ; 72033.062 pg / l. Tissue structure experiencing development of ovarian follicles and  germinal proliferation, proliferation of mammary tissue experiencing the lobules and ducts interlobularis development. Conclusion of the study is the pigeon pea daidzein levels in the blood increased in the 24th hour and potential as a natural estrogen.Key Word         : daidzein, phytoestrogens, ovarian, mammary
Analisis Kadar Gula Reduksi pada Fermentasi Kacang Gude (Cajanus cajan) oleh Aspergillus niger Pujiati, Pujiati; Primiani, C. Novi
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 13, No 1 (2016): Prosiding Seminar Nasional XII Biologi
Publisher : Universitas Sebelas Maret

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Abstract

Pigeonpea (Cajanus cajan) is a tropical legume grown mainly in Indonesia. Though largely considered an orphan crop, pigeonpea has a huge untapped potential for improvement both in quantity and quality of yield. Pigeonpea seeds contain 65 % starch. Based on its starch level can be use as substrate of solid-state fermentation. Solid-state fermentation using Aspergillus niger because they have amylolytic and cellulolytic activity and not produce micotoxin. This study was carried out to know the change reducing sugar level of fermenting pigeonpea as to know a success of fermentation process. Completely randomized design was used, data result were analyzed with ANOVA, then were continued with Least Significant Difference. Different parameters such as inoculums concentration and incubation time. Inoculum concentration of 2 mL/g and incubation time of 72 hours give the best result of 12,50%.Keywords: solid state fermentation, glucose reduction, proteolitic, pigeon pea
Co-Authors . Waris Agita Risma Nurhikmawati Agustina Fajar Rini Agustina Ika Widya Gunita Andarista Diaz Aleydaputri Asna Alfinnisa Asri Musandi Waraulia Aulia Dhita Nanda Aza Ayunda Diamay Banconowati, Mawar Bregas Dede Awalashari C Primiani C. Novi Primiani Cicilia Novi Primiani Cicilia Novi Primiani Cicilia Novi Primiani Cicilia Novi Primiani Cicilia Novi Primiani Cicilia Novi Primiani Darmadi Darmadi Darmadi Davi Apriandi Desi Lia Nur Rahmadani Devi Arindasandy Dewantoro, Tomas Dewi Ratih Tirto Sari Dhio Putra Mahendra Dimas Setiawan Dimas Setiawan Dwi Anggoro Dwi Rosita Sari Ega Rahayu Widodo Fatatus Rizka Nurdiana Gabriella Chandrakirana Krisnamurti Gista Aulia Hakiki Hariyani, D. S Herlina Fitriastuti Imam Tafsir J Handika Khofivah Nurwahid Maulana Khoirunnisa Lili Rahmwati Krisnamurti, Gabriella Chandrakirana Lilik Suryani M Lukitasari Mahda Mumtahanah Marheny Lukitasari Marheny Lukitasari Mohammad Arfi Setiawan Mohammad Arfi Setiawan N K Dewi Nurhikmawati, A. R Nurul Kusuma Dewi Nurul Kusuma Dewi prasetyo, Agus wahyu Pratondo, Bagus Setyo Primiani, C N Primiani, C. Novi Primiani, Cicilia Novi Primiani, Cicillia Novi R Hasan R. Bekti Kiswardianta R. Bekti Kiswardianta, R. Bekti Rafaella Chandraseta Megananda Raras Setyo Retno, Raras Setyo Ristanti, Yuliana Sanusi Sanusi Sanusi Sanusi Sari, Dewi Eka Siti Marhamatul Sri Lestari Sri Utami Sudarmiani Sudarmiani Sudarmiani Sudarmiani W Murtafiah W Widiyanto Wachidatul Linda Yuhanna Weka Sidha Bhagawan Wiwin Solikati Wiwin Solikati, Wiwin Yuhanna, W. L Yunastiti Primanda Hapsari