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Pujiati
Pendidikan Biologi, Fakultas Keguruan Dan Ilmu Pendidikan, Universitas PGRI Madiun
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Journal : Biota

 1 
ANALISA KADAR PROTEIN CRUDE ENZIM SELULASE DARI KAPANG Rhizopuz Sp PADA SUBSTRAT AMPAS TEBU HASIL ISOLASI DARI KEBUN CENGKEH, KARE, MADIUN Pujiati pujiati
Biota Vol 3 No 1 (2017): Vol 3 No 1 Januari 2017
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/Biota.v3i1.930

Abstract

Kapang Rhizopus sp merupakan salah satu mikroorganisme yang memiliki kemampuan tinggi untuk menghasilkan enzim selulase.Enzim selulase merupakan enzim yang dapat menghidrolisis selulosa. Hidrolisis meliputi proses pemecahan polisakarida di dalam biomassa lignoselulosa, yaitu: selulosa dan hemiselulosa menjadi monomer gula penyususnnya. Penelitian ini bertujuan untuk mengetahui produksi dan aktivitas enzim selulase terhadap aktivitas crude enzim selulase dari kapang Rhizopus sp dengan subsrtat ampas tebu (bagase). Metode penelitian menggunakan kuantitatif eksperimen dengan pola rancangan acak lengkap (RAL) dua faktorial. Perlakuan penelitian meliputi perbedan inokulum (K) yaitu 5% (K1), 15% (K2), 25% (K3) dan lama fermentasi (T) yaitu 3hari (T1), 6hari (T2), 9hari (T3), dan 12hari (T4). Data yang diambil dari perlakuan tersebut adalah kadar protein dengan metode brownstead lowry. Analisis data menggunakan variansi anava dua jalur dengan taraf signifikansi 5% setelah itu dilanjutkan dengan uji Beda Nyata Terkecil (BNT ). Hasil penelitian menunjukkan bahwa: Fhit > Ftab sehingga ada pengaruh antara konsentrasi inokulum dan lama fermentasi terhadap aktivitas crude enzim selulase dari kapang Rhizopus sp, Perlakuan perbedaan konsentrasi dan lama fermentasi mendapatkan kadar protein tertinggi 0,715 dengan konsentrasi 25%  dan lama fementasi 25%
Phytochemical screening, in vitro and in silico antibacterial investigation of Elaeocarpus ganitrus extract Cicilia Novi Primiani; Weka Sidha Bhagawan; Pujiati Pujiati; Dewi Ratih Tirto Sari
Biota Vol 10 No 1 (2024): Jurnal Biota 2024
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/Biota.v10i1.20038

Abstract

This study evaluated phytochemical composition, and in vitro and in silico antibacterial activity of Elaeocarpus ganitrus extract. Elaeocarpus ganitrus leaves, seed and fruit powder were extracted with absolute ethanol. Then, the extract was identified phytochemical compounds qualitatively and evaluated the antibacterial activity through in vitro against Staphylococcus aureus and E. coli. Molecular docking was conducted to evaluate the antibacterial mechanism of Elaeocarpus ganitrus extract. Elaeocarpus ganitrus leaves, seeds, and fruits extract presented positive tannin, saponin, cardiac glycoside, quinone, steroids, terpenoids, and anthocyanins. In vitro analysis performed Elaeocarpus ganitrus leaves strong inhibited Staphylococcus aureus growth and medium inhibition against E. coli. structure activity relationship revealed 14 of 72 compounds have high antibacterial activities. molecular docking of 7 compounds showed inhibition activity of D-alanin ligase of Staphylococcus aureus. Those compounds blocked the activity of D-alanine ligase at inhibitor sites of enzyme, and might be disrupted the cell wall synthesis. In conclusion, Elaeocarpus ganitrus contained several phytochemical compounds and has antibacterial activity both in vitro and in silico investigation.
Co-Authors . Waris Agita Risma Nurhikmawati Agustina Fajar Rini Agustina Ika Widya Gunita Andarista Diaz Aleydaputri Asna Alfinnisa Asri Musandi Waraulia Aulia Dhita Nanda Aza Ayunda Diamay Banconowati, Mawar Bregas Dede Awalashari C Primiani C. Novi Primiani Cicilia Novi Primiani Cicilia Novi Primiani Cicilia Novi Primiani Cicilia Novi Primiani Cicilia Novi Primiani Cicilia Novi Primiani Darmadi Darmadi Darmadi Davi Apriandi Desi Lia Nur Rahmadani Devi Arindasandy Dewantoro, Tomas Dewi Ratih Tirto Sari Dhio Putra Mahendra Dimas Setiawan Dimas Setiawan Dwi Anggoro Dwi Rosita Sari Ega Rahayu Widodo Fatatus Rizka Nurdiana Gabriella Chandrakirana Krisnamurti Gista Aulia Hakiki Hariyani, D. S Herlina Fitriastuti Imam Tafsir J Handika Khofivah Nurwahid Maulana Khoirunnisa Lili Rahmwati Krisnamurti, Gabriella Chandrakirana Lilik Suryani M Lukitasari Mahda Mumtahanah Marheny Lukitasari Marheny Lukitasari Mohammad Arfi Setiawan Mohammad Arfi Setiawan N K Dewi Nurhikmawati, A. R Nurul Kusuma Dewi Nurul Kusuma Dewi prasetyo, Agus wahyu Pratondo, Bagus Setyo Primiani, C N Primiani, C. Novi Primiani, Cicilia Novi Primiani, Cicillia Novi R Hasan R. Bekti Kiswardianta R. Bekti Kiswardianta, R. Bekti Rafaella Chandraseta Megananda Raras Setyo Retno, Raras Setyo Ristanti, Yuliana Sanusi Sanusi Sanusi Sanusi Sari, Dewi Eka Siti Marhamatul Sri Lestari Sri Utami Sudarmiani Sudarmiani Sudarmiani Sudarmiani W Murtafiah W Widiyanto Wachidatul Linda Yuhanna Weka Sidha Bhagawan Wiwin Solikati Wiwin Solikati, Wiwin Yuhanna, W. L Yunastiti Primanda Hapsari