Anak Agung Gde Oka Dharmayudha
Laboratorium Bedah Dan Radiologi Veteriner, Universitas Udayana

Published : 15 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 15 Documents
Search

Case of Entamoebiasis in Pigs Raised with a Free Range Systems in Bali, Indonesia (KASUS ENTAMOEBIASIS PADA BABI YANG DIPELIHARA DENGAN CARA DIUMBAR DI BALI, INDONESIA) Kadek Karang Agustina; Anak Agung Gde Oka Dharmayudha; Ida Bagus Made Oka; I Made Dwinata; I Made Kardena; Nyoman Sadra Dharmawan; I Made Damriyasa
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (125.329 KB)

Abstract

This research aims were to measure the prevalence of Entamoeba in pigs in Bali and to identify thezoonotic potential species of Entamoeba. A total of 183 pig stool samples from Bali have been examined.The method being used in this study were combination between coproscopy and molecular techniques.Concentration sedimentation with Sodium Acetic Formaldehide (SAF) as a solution was used in thecoproscopy method, while the Polimerase Chain Reaction method was used to amplify DNA of Entamoeba.Extracted sample’s DNA examined by using primers that specifically for Entamoeba: Entam 1 (F) (5’-GTTGAT CCT GCC AGT ATT ATA TG-3’) and Entam 2 (R) (5’-CAC TAT TGG AGC TGG AAT TAC-3), and toidentify the zoonotic potential species of Entamoeba, samples that produce 550 bp in first amplificationcontinued by primers Epolecki1 (F) (5’-TCG ATA TTT ATA TTG ATT CAA ATG-3’) and Epolecki2 (R) (5’-CCT TTC TCC TTT TTT TAT ATT AG-3’). The results showed that 76.6% of samples were positive incoproscopical examination, but 84.7 % produced 550 bp bands on PCR amplification by using generalprimers. All positive samples on the first PCR continued to second PCR used specific primers for E.poleckii as a potential zoonotic disease and all of the samples showed negative results. This datademonstrated that the prevalence of Entamoeba in a traditional pig scavenging systems in Bali was 84.7%but no specific infection infection caused by E. polecki was found.
Radiographic Evaluation of Rabbit Femur Implanted Bali Cattle Bone Graft I Wayan Wirata; Steven Dwi Purbantoro; Luh Made Sudimartini; IWN Fajar Gunawan; AA Oka Dharmayudha; IGAG Putra Pemayun
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (107.401 KB)

Abstract

Bone xenograft from cattle bone is commonly used to treat a comminuted fracture case. This study aims to know the process of fractured-femur bone healing in rabbit post-implantation powder bone graft from cortical femur bone of Bali cattle evaluated by radiographs. Ten male local rabbits were used in this study, which were divided into 2 groups randomly. Group I (KI) as control, the diaphysis of femur bone was drilled with a diameter of 5 mm without implanting the bone graft, while Group II (KII), the diaphysis of femur bone was drilled 2 holes with a diameter of 5 mm each and with distance 20 mm, substituted with mineralized powder bone graft for the proximal hole (KIIa) and demineralized powder bone graft for the distal hole (KIIb). Fracture healing evaluation was done at week 0 (24 hours), 2, 4, and 6 postoperative by monitoring the growth of callus, fracture line, and union process with radiograph based classification according to Hammer et al., tabulated statistically, and presented descriptively. The results showed that KI and KII were in the sequel of fracture healing but had not reached remodeling phase perfectly. In conclusion, mineralized and demineralized powder bone graft used in this study was as osteoconductive and the use of bone graft shows no different significance and time shows different significance to fracture healing.
The Vermicidal Effectivity of Wudani Leaf Extract (Quisqualis indica Linn) in Treating Infection caused by Gastrointestinal Worms in Cattle Ida Bagus Komang Ardana; Made Suma Anthara; Dan Anak Agung Gde Oka Dharmayudha; DK Harya Putra
Journal of Global Pharma Technology .
Publisher : Journal of Global Pharma Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The present study was aimed to study whether application of 100% extract of wudani leaf (Quisqualis indica Linn) was able to kill gastrointestinal worms Strongylus Sp. (vermicidal effect). In order to make comparation with commercial drug commonly used, one group was treated with Rheindazol (100 ml of Rheindazol contain 10 gram of albendazol) at a single dose of 10 mg/kg of body weight (BW). The other 3 groups were subjected to a single treatment with 100% wudani leaf extract at 10 mg, 15 mg, and 20 mg/kg of BW, respectively. Assessment on its vermicidal effect was carried out by conducting fecal egg count reduction (FECR). The results showed that the vermicidal property of 100% wudani leaf extract given at 10mg/kg BW was 100% and this was significantly (P < 0.01) higher than that of dose of 15 kg/kg BW (92.4%), 20 mg/kg BW (96%) and even compared to that of albendazol treatment (96%). Thus, from the present in vivo study, it can be concluded that 100% wudani leaf extract due to its vermicidal property can be effectively used to cure gastrointestinal worm infection and, therefore, can be subsequently applied to overcome worm infection in cattle. Keywords: Gastrointestinal worm, Wudani leaf extract, Vermicidal property.
Evaluation of Acute Dermal Toxicity of Hibiscus Leaves as Simplicial Ointment on Albino Rats Saputra, Made Gede Adi Surya; Sudira, I Wayan; Samsuri, Samsuri; Merdana, I Made; Dharmayudha, Anak Agung Gde Oka; Sudisma, I Gusti Ngurah
Jurnal Medik Veteriner Vol. 6 No. 2 (2023): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss2.2023.216-229

Abstract

Hibiscus is a plant that has been shown to contain substances that may be used as sunscreen to protect the skin from UV radiation. In terms of their potential as sunscreens, flavonoids, tannins, and quinone chemicals are crucial because they are antioxidants that can reduce the negative effects of UV radiation and boost photoprotective activity. This study aimed to investigate the acute dermal toxicity potential of hibiscus leaves simplicial ointment 40% on female albino rats. The evaluation method refered to The OECD Guideline for Testing of Chemicals – No. Test: 402, Acute Dermal Toxicity-Fixed Doses Procedure. Based on the Globally Harmonized System compared to this study reported that acute dermal median lethal dose (LD50) was > 2000 mg/kg. It can be concluded that hibiscus leaves simplicial ointment 40% has safe to apply topically and doesn't produce acute skin toxicity.
Evaluasi Salep Simplisia Daun Kembang Sepatu yang Memiliki Potensi Terhadap Pertumbuhan Rambut Arinata, I Ketut Teguh; Sudira, I Wayan; Samsuri, Samsuri; Merdana, I Made; Dharmayudha, Anak Agung Gde Oka
Majalah Farmasetika Vol 10, No 2 (2025)
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/mfarmasetika.v10i2.59421

Abstract

Berbagai studi mengenai potensi kembang sepatu sebagai tanaman obat yang memiliki pengaruh terhadap pertumbuhan rambut telah dilaporkan. Pada penelitian ini dibuat formulasi sediaan topikal yaitu salep dari simplisia daun kembang sepatu dengan bahan dasar hidrokarbon. Pengujian dilakukan untuk melihat kualitas fisik dari sediaan. Oleh karena itu, penelitian ini bertujuan untuk mengetahui pengaruh konsentrasi simplisia terhadap perolehan kualitas salep yang baik serta melihat potensi pembuatan salep menggunakan bahan dasar simplisia daun kembang sepatu. Simplisia daun kembang sepatu dicampur dengan bahan dasar salep hidrokarbon yaitu vaselin alba sehingga membentuk formulasi dengan konsentrasi simplisia yang bervariasi. Pengujian yang dilakukan meliputi uji organoleptik, uji homogenitas, uji pH, uji daya sebar, uji daya lekat, dan cycling test. Hasil penelitian menunjukkan tidak ada perubahan secara organoleptik, tidak homogen, pH 6 yang sesuai dengan pH kulit, daya sebar di bawa standar menyebabkan konsistensi padat, tiga dari empat formulasi lolos standar daya lekat, dan hasil cycling test menunjukkan semua formulasi stabil pada proses penyimpanan. Berdasarkan hasil penelitian dapat disimpulkan konsentrasi simplisia daun kembang sepatu berpengaruh terhadap kualitas salep. Namun, sediaan salep tersebut belum memenuhi standar homogenitas dan daya sebar.