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Potensi Serat Pangan Proso Milet (Panicum miliaceum L.) Terpraproses dalam Menstimulasi Pertumbuhan Lactobacillus rhamnosus SKG34 Kartika Pratiwi, I Desak Putu; Diah Puspawati, Gusti Ayu Kadek; Nocianitri, Komang Ayu
JURNAL AGROTEKNOLOGI Vol 17 No 01 (2023)
Publisher : Faculty of Agricultural Technology, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/j-agt.v17i01.27811

Abstract

Proso millet (Panicum miliaceum L.) was evaluated as a source of dietary fiber. Preprocessing of millet flour could increase dietary fiber. The dietary fiber is considered to provide benefits as a prebiotic. The purpose of this study was to analyze the effect of preprocessing on millet flour dietary fiber and its ability to stimulate the growth of probiotic Lactobacillus rhamnosus SKG34. The research was conducted in two stages, i.e. preprocessing millet flour and viability of probiotic bacteria (Lactobacillus rhamnosus SKG34) on different broth media. First, millet flour was made in various preprocessed method: without preprocessing, germination preprocessing, fermentation preprocessing, germination – fermentation preprocessing. Second, the viability of Lactobacillus rhamnosus SKG34 was carried out on various media broth: glucose-free MRS (control), MRS, adding dietary fibers extract from millet flour with different preprocessing. The results showed that millet flour with fermented preprocessing (F) provided the highest dietary fiber of 22.38% with a water content of 11.97% and a tannin content of 0.08%. L. rhamnosus SKG34 exhibited the highest viability when grown in MRS broth. The addition of dietary fiber extract did not significantly enhance the viability of L. rhamnosus SKG34 compared to the glucose-free MRS broth (control), which showed a viability of 5 log10 CFU/mL. Thus, it can be concluded that millet preprocessing with fermentation (F) can significantly increase the dietary fiber of millet flour. However, dietary fiber millet has no potential as a suitable prebiotic candidate for promoting probiotic bacterial growth. Keywords: dietary fiber, lactic acid bacteria, millet flour, prebiotic, preprocessed
Pengaruh Perbandingan Konsentrasi Tepung Agar-Agar dan Biji Chia (Salvia Hispanica L.) terhadap Sifat Fisikokimia dan Sensori Fruit Leather Terong Belanda (Solanum Betaceum Cav.) Anggraini, Fitri Dwi; Puspawati, Gusti Ayu Kadek Diah; Nocianitri, Komang Ayu
Jurnal Ilmu dan Teknologi Pangan (ITEPA) Vol 13 No 2 (2024): Jurnal ITEPA
Publisher : Study Program of Food Technology, Faculty of Agricultural Technology, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/itepa.2024.v13.i02.p05

Abstract

Fruit leather is a processed dried fruit in the form of thin sheets that can be rolled up. One of the fruits that can be processed into fruit leather is dutch eggplant. The elasticity of fruit leather is influenced by the binder used. The use of seaweed pulp binder in dutch eggplant fruit leather requires a long preparation time. One of the natural ingredients that has the potential to be used as a binder and is easy to prepare is chia seeds and agar-agar. The research was aimed to determine the effect of the concentration ratio of agar-agar powder and chia seeds on the physicochemical and sensory characteristics of dutch eggplant fruit leather and to determine the appropriate concentrations of agar-agar powder and chia seeds to produce dutch eggplant fruit leather with the best physicochemical and sensory characteristics. This study used a completely randomized design with a comparison of the concentrations of agar powder and chia seeds consisting of 6 levels: 100%:0%, 80%:20%, 60%:40%, 40%:60%, 20% :80%, and 0%:100% treatment was repeated 3 times. The data was analyzed with analysis of variance, and if the treatment had a significant effect, then followed by the Duncan's Multiple Range Test. The results showed that the concentration of agar-agar and chia seeds had a significant effect on tensile strength, L,a*,b* values, water content, crude fiber, flavonoids content, antioxidant activity, and sensory including hedonic tests (color, texture, overall acceptance). and scoring (texture). The best dutch eggplant fruit leather was treated with a ratio of agar- agar powder and chia seeds concentration of 40%:60% with a tensile strength value 2.18 MPa, L value 19.6, a* 15.36, b* 13.16, water content 13.55%, crude fiber 7.47%, flavonoids content 3.41mgQE/g, antioxidant activity 53.93%, color, taste, plastic texture and overall acceptance liked.
Isolasi dan Karakterisasi Bakteri Asam Laktat (BAL) pada Sere Kedele di Kabupaten Gianyar Sandhi Wipradnyadewi, Putu Ari; Sugitha, I Made; Wayan Gunam, Ida Bagus; Nocianitri, Komang Ayu
Jurnal Ilmu dan Teknologi Pangan (ITEPA) Vol 13 No 2 (2024): Jurnal ITEPA
Publisher : Study Program of Food Technology, Faculty of Agricultural Technology, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/itepa.2024.v13.i02.p11

Abstract

Lactic acid bacteria (LAB) are found in many fermented food products. Sere kedele is a fermented soybean food produced by the people of Gianyar Regency. This study aims to isolate and characterize LAB on sere kedele in Gianyar Regency. Saturated sampling technique was used in sampling Sere Kedele in Gianyar Regency. The research results showed that there were 24 isolates that were purple in color and were LAB isolates in the form of cocci and bacilli.
Viabilitas probiotik Lacticaseibacillus rhamnosus SKG34 pada sari buah terung belanda (Solanum betaceum Cav.) terfermentasi selama penyimpanan pada suhu dingin Nocianitri, Komang Ayu; Pratiwi, I Desak Putu Kartika; Wulandari, Kristina; Ramona, Yan; Sujaya, I Nengah
AGROINTEK Vol 19, No 3 (2025)
Publisher : Agroindustrial Technology, University of Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrointek.v19i3.26535

Abstract

Tamarillo (Solanum betaceum Cav.) is a tropical fruit that has the potential to be developed into functional food in the form of fruit juice containing probiotics. In order to develop the probiotic-containing product, the suitability of fruit juice to support the growth of probiotics and the viability of probiotics during the fermentation and storage processes are the prime concerns. Therefore, this study aims to determine the viability of the local probiotic Lacticaseibacillus rhamnosus SKG34 and the characteristics of fermented Tamarillo juice during storage at 4oC. Fermented tamarillo juice containing L. rhamnosus SKG34 is produced by fermenting Tamarillo juice with L. rhamnosus SKG34 and then stored at a temperature of ±4oC for 30 days. Population of L. rhamnosus SKG34, total sugar, total acid, pH, vitamin C, antioxidant capacity, and sensory characteristics were determined at 0, 5, 10, 15, 20, 25, and 30 days. The results showed that the probiotic L. rhamnosus SKG34 in fermented tamarillo juice was able to survive for 30 days with a population of 2.58 x 1010 CFU/ml from the initial population of 9.54 x 1010 CFU/ml. However, considering that chemical characteristics and other functional properties also influence the acceptability of fermented tamarillo juice, the optimal storage time is 20 days at 4oC. Under this condition, the fermented tamarillo juice containing 3.6 x 1010 CFU/ ml of L. rhamnosus SKG34; 11.23% sugars; total acid 0.98% acids; pH 3.44; 31.76 mg/100 ml vitamin C; and 4,725 GAEC/100 ml of antioxidant capacity, with favorable sensory levels.
Aktivitas Antioksidan Lactobacillus Rhamnosus FBB Secara in Vitro Husni Mubarok; Komang Ayu Nocianitri; I Dewa Gede Mayun Permana
Jurnal Ilmiah Teknologi Pertanian Agrotechno Vol. 4 No. 1 (2019): April
Publisher : Fakultas Teknologi Pertanian, Universitas Udayana Gedung GA, Fakultas Teknologi Pertanian, Universitas Udayana Jl. Raya Bukit Jimbaran, Jimbaran, Kuta Selatan, Bali Telp/Fax: (0361) 701801

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JITPA.2019.v04.i01.p01

Abstract

Probiotik merupakan organisme hidup yang mampu memberikan efek yang menguntungkan bagi kesehatan inangnya. Lactobacillus rhamnosus yang diisolasi dari feses bayi sehat merupakan bakteri asam laktat (BAL) yang diketahui memiliki potensi sebagai probiotik. Tujuan dari penelitian ini adalah untuk mengetahui aktivitas antioksidan dari isolat L. rhamnosus FBB secara in vitro. Isolat L. rhamnosus FBB tersebut antara lain: L. rhamnosus FBB nomor 21, 26, 52, 59, 75, dan 81. Uji konfirmasi menunjukkan bahwa keseluruhan isolat L. rhamnosus FBB termasuk bakteri homofermentatif, bakteri gram positif, katalase negatif dan memiliki bentuk morfologi batang berantai. Aktivitas antioksidan ditentukan dengan mengukur kemampuan penangkapan radikal bebas 1,1-diphenyl-2-picrylhydrazil (DPPH) dengan membandingkan subjek penelitian yakni supernatant, intact cell dan cell lysate. Hasil penelitian menunjukkan bahwa penangkapan radikal bebas pada supernatant, intact cell dan cell lysate masing-masing adalah 39,00-66,71%, 15,76-21,28% dan 4,79-10,86%. L. rhamnosus FBB 75 dan L. rhamnosus FBB 81 menunjukkan aktivitas antioksidan tertinggi dibandingkan dengan isolat lainnya. Aktivitas penangkapan radikal bebas DPPH pada supernatant, intact cell dan cell lysate pada L. rhamnosus FBB 75 adalah 66,71%, 15,76%, 10,86% dan aktivitas antioksidan pada L. rhamnosus FBB 81 adalah 66,43%, 21,28% 9,37%. Hasil penelitian ini membuktikan bahwa L. rhamnosus FBB 75 dan L. rhamnosus FBB 81 merupakan strain yang potensial untuk dikembangkan sebagai probiotik antioksidan.
Pengaruh Waktu Penambahan Lactobacillus rhamnosus SKG 34 terhadap Karakteristik Tape Ketan Probiotik Selama Penyimpanan Emilya Grasiana Dede; Komang Ayu Nocianitri; Luh Putu Trisna Darmayanti
Jurnal Ilmiah Teknologi Pertanian Agrotechno Vol. 3 No. 1 (2018): April
Publisher : Fakultas Teknologi Pertanian, Universitas Udayana Gedung GA, Fakultas Teknologi Pertanian, Universitas Udayana Jl. Raya Bukit Jimbaran, Jimbaran, Kuta Selatan, Bali Telp/Fax: (0361) 701801

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JITPA.2018.v03.i01.p02

Abstract

Tape is one of Indonesia's traditional fermented food that has a sweet and slightly sour taste. The basic ingredients of tape are materials that contain carbohydrates such as cassava and sticky rice. Lactobacillus rhamnosus SKG 34 is one type of lactic acid bacteria isolated from Sumbawa wild horse milk. This study aims to determine the best time addition of Lactobacillus rhamnosus SKG 34 and to determine the effect of the addition of Lactobacillus rhamnosus SKG 34 on the characteristic of probiotics Tape Ketan during storage. The study was conducted in two parts, namely first parts which aimed to know the difference of probiotic addition of Lactobacillus rhamnosus SKG 34 before fermentation and after fermentation. Second research was aimed to find out the viability of Lactobacillus rhamnosus SKG 34 in probiotic tape Ketan and characteristics of tape ketan during storage using Completely Randomized Design with the treatment of storage consisting of 5 levels were day 0, day 1, day 2, day 3 and day 4. The variables were LAB total, sugar total, acid total, pH and sensory characteristics. The results of this research showed that the addition of probiotics before fermentation had a higher LAB than after fermentation (3.5 x 108 cfu /g). Probiotic tape Ketan still has a good characteristic until third day storage with characteristic LAB total 9,0 x 108 cfu/g, sugar total 8,17%, acid total 0,76%, pH 3,54 and color (rather like), Flavor (rather like), texture (rather like), sense hedonic test (rather like), sense scoring test (neutral) and overall acceptance (rather like).
Bile Salt Hydrolase pada Probiotik sebagai Agen Penurun Kolesterol: Kajian Pustaka Kristina Wulandari; Noval Wahyu Adi; Ida Ayu Agung Prawitasari; Ni Nengah Dwi Fatmawati; Komang Ayu Nocianitri; I Nengah Sujaya
Metamorfosa: Journal of Biological Sciences Vol. 11 No. 2 (2024)
Publisher : Program Magister Ilmu Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2024.v11.i02.p1

Abstract

Dislipidemia menjadi salah satu faktor pemicu risiko penyakit metabolit dan beban kesehatan global. Tingginya kadar kolesterol total atau LDL pada jaringan kardiovaskular menjadi kasus dislipidemia yang kerap dijumpai di masyarakat. Salah satu metode alternatif dalam mencegah dan mengatasi masalah kolesterol tinggi adalah dengan memanfaatkan probiotik melalui aktivitas Bile Salt Hydrolase (BSH). BSH merupakan enzim yang berperan dalam pembentukan garam empedu bebas yang dapat menyebabkan peningkatan sintesis de novo pada asam empedu hepatik. Kehadiran BSH akan mengganggu siklus enterohepatik dengan menghidrolisis ikatan amida pada asam empedu terkonjugasi menjadi asam empedu bebas dengan perubahan sifat menjadi lebih hidrofobik dan sedikit diserap kembali oleh tubuh, sehingga akan lebih banyak dikeluarkan melalui feses. Semakin banyak ekskresi asam empedu melalui feses, maka semakin sedikit asam empedu yang dibawa kembali ke hati melalui siklus enterohepatik, sehingga untuk menggantikan asam empedu yang hilang, diperlukan jumlah kolesterol yang lebih banyak untuk sintesis de novo di hati. Lebih lanjut, kehadiran BSH juga dapat memicu mekanisme penurunan kolesterol lain seperti kopresipitasi kolesterol dengan garam empedu terdekonjugasi serta penurunan efisiensi pelarutan dan penyerapan lipid di usus. Saat ini, bakteri yang memiliki BSH tidak hanya berasal dari saluran pencernaan manusia seperti Lactobacillus dan Bifidobacteria, tetapi dapat juga ditemukan pada bakteri makanan fermentasi dan bakteri patogen seperti Listeria monocytogenes dan Brucella abortus. Perlu diperhatikan bahwa setiap strain bakteri memiliki katalisator, kemampuan serta jenis dan jumlah gen BSH yang spesifik. Dengan perkembangan aplikasi probiotik dalam dasa warsa terakhir, kajian pustaka ini diharapkan dapat memberikan pandangan terkini tentang peranan probiotik dalam menurunkan kolesterol. Kata Kunci: dislipidemia, LDL, asam empedu, siklus enterohepatik
Bile Salt Hydrolase pada Probiotik sebagai Agen Penurun Kolesterol: Kajian Pustaka Kristina Wulandari; Noval Wahyu Adi; Ida Ayu Agung Prawitasari; Ni Nengah Dwi Fatmawati; Komang Ayu Nocianitri; I Nengah Sujaya
Metamorfosa: Journal of Biological Sciences Vol. 11 No. 2 (2024)
Publisher : Program Magister Ilmu Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2024.v11.i02.p1

Abstract

Dislipidemia menjadi salah satu faktor pemicu risiko penyakit metabolit dan beban kesehatan global. Tingginya kadar kolesterol total atau LDL pada jaringan kardiovaskular menjadi kasus dislipidemia yang kerap dijumpai di masyarakat. Salah satu metode alternatif dalam mencegah dan mengatasi masalah kolesterol tinggi adalah dengan memanfaatkan probiotik melalui aktivitas Bile Salt Hydrolase (BSH). BSH merupakan enzim yang berperan dalam pembentukan garam empedu bebas yang dapat menyebabkan peningkatan sintesis de novo pada asam empedu hepatik. Kehadiran BSH akan mengganggu siklus enterohepatik dengan menghidrolisis ikatan amida pada asam empedu terkonjugasi menjadi asam empedu bebas dengan perubahan sifat menjadi lebih hidrofobik dan sedikit diserap kembali oleh tubuh, sehingga akan lebih banyak dikeluarkan melalui feses. Semakin banyak ekskresi asam empedu melalui feses, maka semakin sedikit asam empedu yang dibawa kembali ke hati melalui siklus enterohepatik, sehingga untuk menggantikan asam empedu yang hilang, diperlukan jumlah kolesterol yang lebih banyak untuk sintesis de novo di hati. Lebih lanjut, kehadiran BSH juga dapat memicu mekanisme penurunan kolesterol lain seperti kopresipitasi kolesterol dengan garam empedu terdekonjugasi serta penurunan efisiensi pelarutan dan penyerapan lipid di usus. Saat ini, bakteri yang memiliki BSH tidak hanya berasal dari saluran pencernaan manusia seperti Lactobacillus dan Bifidobacteria, tetapi dapat juga ditemukan pada bakteri makanan fermentasi dan bakteri patogen seperti Listeria monocytogenes dan Brucella abortus. Perlu diperhatikan bahwa setiap strain bakteri memiliki katalisator, kemampuan serta jenis dan jumlah gen BSH yang spesifik. Dengan perkembangan aplikasi probiotik dalam dasa warsa terakhir, kajian pustaka ini diharapkan dapat memberikan pandangan terkini tentang peranan probiotik dalam menurunkan kolesterol. Kata Kunci: dislipidemia, LDL, asam empedu, siklus enterohepatik
Karakteristik Fisik dan Sensoris Keju Tomme Probiotik dengan Variasi Perbandingan Kultur Probiotik Lokal Paramita Wulandari, Kadek Ratih; Nocianitri, Komang Ayu; Suparthana, I Putu; Sutriswati Rahayu, Endang
Itepa : Jurnal Ilmu dan Teknologi Pangan Vol. 14 No. 3 (2025): ITEPA
Publisher : Study Program of Food Technology, Faculty of Agricultural Technology, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/itepa.2025.v14.i03.p12

Abstract

Abstract Tomme cheese is a type of ripened cheese with a semi-hard texture and is covered by mold and has the characteristics of a yellowish cheese rind, a dense structure with an elastic texture, an earthy aroma and a predominantly salty taste. Tomme cheese is suitable as a probiotic food because it can maintain sufficient probiotic cell viability. The types of local probiotic cultures that can be used are Lactiplantibacillus plantarum subs. plantarum Dad-13 and pH-lowering bacteria, namely Streptococcus thermophilus Dad-11. The purpose of this study was to determine the effect of the ratio of Lactiplantibacillus plantarum subs. plantarum Dad-13 and S. thermophilus Dad-11 on the physical and sensory characteristics of probiotic tomme cheese and to determine the ratio that produces the best physical and sensory characteristics. This study used a Randomized Block Design (RBD) with a treatment comparison of Lactiplantibacillus plantarum subs. plantarum Dad-13 and S. thermophilus Dad-11 cultures. plantarum Dad-13 and S. thermophilus Dad-11 with 3 levels, namely 1:1, 1:2, and 1:3. The treatment was then repeated 3 times, so that 9 experimental units were obtained. The data obtained were analyzed using Kruskal Wallis and continued with the Dunn test (with Bonferroni correction) in the SPSS application. The results showed that the comparison of local starter cultures affected the yield, hardness value, color intensity, and sensory characteristics of tomme cheese. Probiotic tomme cheese with a starter culture ratio of 1:3 had the best physical and sensory characteristics with a yield value of 10.63%, a hardness value of 33.89 N, color intensity L* 71.67, a* value of 0.97, b* value of 23.5 which indicated a yellowish cream color with the name butterscotch, with sensory characteristics of color that was very liked, aroma, texture, and taste that was liked with a very hard texture and a very salty taste and overall acceptance was liked. Keywords: tomme cheese, probiotic, Streptococcus thermophilus Dad-11, and Lactiplantbacillus plantarum subs. plantarum Dad-13 Abstrak Keju tomme merupakan jenis keju peram dengan tekstur semi-keras dan diselimuti oleh jamur dan memiliki karakteristik kulit keju berwarna kekuningan, struktur padat dengan tekstur elastis, aroma earthy dan didominasi oleh rasa asin. Keju tomme cocok dijadikan sebagai pangan probiotik karena dapat menjaga viabilitas sel probiotik yang cukup. Jenis kultur probiotik lokal yang dapat digunakan adalah Lactiplantibacillus plantarum subs. plantarum Dad-13 dan bakteri penurun pH yaitu Streptococcus thermophilus Dad-11. Tujuan dari penelitian ini yaitu untuk mengetahui pengaruh perbandingan Lactiplantibacillus plantarum subs. plantarum Dad-13 dan S. thermophilus Dad-11 terhadap karakteristik fisik dan sensoris keju tomme probiotik serta untuk mengetahui perbandingan yang menghasilkan karakteristik fisik dan sensoris terbaik. Penelitian ini menggunakan Rancangan Acak Kelompok (RAK) dengan perlakuan perbandingan kultur Lactiplantibacillus plantarum subs. plantarum Dad-13 dan S. thermophilus Dad-11 dengan 3 taraf yaitu 1:1, 1:2, dan 1:3. Perlakuan kemudian diulang sebanyak 3 kali, sehingga diperoleh 9 satuan percobaan. Data yang diperoleh dianalisis dengan Kruskal Wallis dan dilanjutkan dengan uji Dunn (dengan koreksi Bonferroni) pada aplikasi SPSS. Hasil penelitian menunjukkan perbandingan kultur starter lokal berpengaruh terhadap rendemen, nilai kekerasan, intensitas warna, dan karakteristik sensoris keju tomme. Keju tomme probiotik dengan perbandingan kultur starter 1:3 memiliki karakteristik fisik dan sensoris terbaik dengan nilai rendemen 10,63%, nilai kekerasan 33,89 N, intensitas warna L* 71,67, nilai a* 0,97, nilai b* 23,5 yang menunjukkan warna krem kekuningan dengan nama butterscotch, dengan karakteristik sensoris warna sangat disukai, aroma, tekstur, dan rasa disukai dengan tekstur sangat keras dan rasa sangat asin serta penerimaan keseluruhan disukai. Kata Kunci: keju tomme, probiotik, Streptococcus thermophilus Dad-11, dan Lactiplantbacillus plantarum subs. plantarum Dad-13
Pengaruh Ekstrak Jeruk Nipis (Citrus aurantifolia) terhadap Karakteristik Keju Lunak dengan Milk Clotting Enzyme Lactobacillus rhamnosus SKG 34 Listia Liantania, Kadek; Mayun Permana, I Dewa Gde; Nocianitri, Komang Ayu
Itepa : Jurnal Ilmu dan Teknologi Pangan Vol. 14 No. 4 (2025): ITEPA
Publisher : Study Program of Food Technology, Faculty of Agricultural Technology, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/itepa.2025.v14.i04.p05

Abstract

Abstract Cheese production involves coagulating milk proteins through the use of coagulating agents such as enzymes and acids. The Milk Clotting Enzyme (MCE) from Lactobacillus rhamnosus SKG 34 can serve as an alternative to rennet in cheese manufacturing processes, although the resulting soft cheese exhibits low yield and protein content. The addition of acid can assist the coagulation process, thereby improving the quality of the resulting cheese. Lime extract is expected to lower the pH of milk and optimize the activity of the enzyme, thus enhancing the characteristics of the soft cheese produced. This study was conducted to evaluate how lime extract influences the properties of soft cheese made using milk clotting enzyme derived from Lactobacillus rhamnosus SKG 34, as well as to identify the most effective concentration of lime extract for achieving optimal soft cheese quality. This study used a Randomized Block Design (RBD) with five concentrations of lime extract (0%, 2%, 4%, 6%, and 8%) repeated three times, creating 15 experimental units. Yield, pH, moisture content, protein content, hardness, and sensory evaluation were observed as research variables. ANOVA was used for data analysis, with Duncan's test as a follow-up analysis when treatments showed significant effects (P<0.05). The results showed that adding 4% lime extract produced the best soft cheese using MCE L. rhamnosus SKG 34 with pH 4,19, moisture content 57,77%, protein content 16,44%, yield 21,52%, hardness 3,15N, and hedonic values for color, aroma, taste, texture, and overall acceptance preferred by panelists. Keywords: lime extract, soft cheese, Lactobacillus rhamnosus SKG 34, milk clotting enzyme Abstrak Proses pembuatan keju dilakukan melalui koagulasi protein susu menggunakan agen penggumpal seperti enzim dan asam. Milk Clotting Enzyme (MCE) dari bakteri Lactobacillus rhamnosus SKG 34 dapat digunakan sebagai alternatif pengganti rennet dalam produksi keju, akan tetapi keju lunak yang dihasilkan memiliki rendemen dan kadar protein yang rendah. Penambahan asam dapat membantu dalam koagulasi susu sehingga keju yang dihasilkan lebih optimal. Penambahan ekstrak jeruk nipis diharapkan dapat menurunkan pH susu dan mengoptimalkan aktivitas enzim sehingga meningkatkan karakteristik keju lunak yang dihasilkan. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak jeruk nipis terhadap karakteristik keju lunak dengan MCE L. rhamnosus SKG 34 serta mengetahui konsentrasi ekstrak jeruk nipis paling tepat sehingga menghasilkan keju lunak dengan karakteristik terbaik. Penelitian ini menerapkan Rancangan Acak Kelompok (RAK) dengan lima taraf konsentrasi ekstrak jeruk nipis (0%, 2%, 4%, 6%, dan 8%) yang diulang sebanyak tiga kali menghasilkan 15 unit percobaan. Rendemen, pH, kadar air, kadar protein, kekerasan, dan evaluasi sensoris diamati sebagai variabel penelitian. ANOVA digunakan untuk analisis data, dengan uji Duncan sebagai analisis lanjutan ketika perlakuan memberikan pengaruh signifikan (P<0,05). Hasil penelitian menunjukkan penambahan ekstrak jeruk nipis 4% menghasilkan keju lunak terbaik menggunakan MCE L. rhamnosus SKG 34 dengan pH 4,19, kadar air 57,77%, protein 16,44%, rendemen 21,52%, kekerasan 3,15N, serta nilai hedonik warna, aroma, rasa, tekstur, dan penerimaan keseluruhan yang disukai panelis. Kata kunci: ekstrak jeruk nipis, keju lunak, Lactobacillus rhamnosus SKG 34, milk clotting enzyme
Co-Authors A.A. Gd. Tryadi Pradipta Adiel Kunti Permatasari Aditya Yusril Hidayat Agnes Prina Rini Agus Selamet Duniaji Agus Slamet Djuniaji Anak Agung Istri Sri Wiadnyani Andreas Kurniawan Anggraini, Fitri Dwi Anjani Fatnasari Anton Anugrah Ave Regina Rosari Desak Putu Widya Puspasari Dewa Ayu Tri Ulandari Dewa Gede Eka Prayoga Dina Puti Kartika Dwi Ariesta Wijaya Putra Dyta Aprida Asendy Edwin Sam Putra Surbakti Emilya Grasiana Dede Emilya Grasiana Dede Fahmy Kafa Hakiki Febriyawati Cahyanty Nugraha Fiensa Forsalina Fukuda Kenji Gede Pracheta Febricia Ginanjar Rifai Gusti Ayu Kadek Diah Puspawati Gusti Chandra Rizki Gusti Putu Adi Wira Kusuma Husni Mubarok I D.P. Kartika Pratiwi I Dewa Gde Mayun Permana I Dewa Gde Mayun Permana I Dewa Gede Mayun Permana I Dewa Gede Natih Kacu Putra I Dewa Made Sukrama I Gusti Ayu Ekawati I Gusti Putu Bhuana Aristya Putra I K. Suter I Ketut Peri Andika I Ketut Suter I Made Anom Sutrisna Wijaya I Made Edi Widiawan I Made Sugitha I MADE SUPARTHA UTAMA I Nengah Kencana Putra I Nengah Sujaya I Nengah Sujaya I Putu Suparthana I Putu Surya Wirawan I W. R. Widarta I Wayan Aditya I Wayan Agus Widyantara I Wayan Rai Widarta I.D.P.K. Pratiwi Ida Ayu Agung Prawitasari Ida Ayu Agung Prawitasari Ida Bagus Wayan Gunam Irvan Kurniawan Simatupang IWR Widarta Jeremy Louis Adisurya Jimmy Fernando Pangaribuan Jordan Dandy Prayoga Juwita Mayningsih Andari Putri Karina Dinda Putri Karlina Ayu Putri Dhahana Komang Puspita Dewi Kristina Wulandari Kristina Wulandari Listia Liantania, Kadek LPIP Sari Luh Komang Puspa Handayani Luh Putu Trisna Darmayanti Made Aditya Dharma Melda Yanti Naibaho Muhamad Mukhlis Prastianto Muhammad Taufiq Aldianto N.W.A. Utami Netta Clarita Tampinongkol Ni Ketut Ayu Krisna Devi Ni Komang Dewi Cinderela Ni Komang Tri Handayani Ni Luh Ari Yusasrini Ni Luh Ketut Ayu Gayatri Pradnya Andini Ni Luh Putu Gita Darmasari Ni Luh Putu Suariani Ni Made Gress Rakasari Nomer Ni Made Indri Hapsari Arihantana Ni Made Miradita Lestari Ni Made Utami Dwipayanti Ni Made Yusa Ni Nengah Dwi Fatmawati Ni Nengah Dwi Fatmawati Ni Nyoman Puspawati Ni Putu Ardi Ningsih Eka Putri Ni Putu Destriani Devi Ni Putu Desy Aryantini Ni Putu Diah Cahyani Subamia Ni Putu Suariani Ni Putu Widarini Ni Wayan Nursini Ni Wayan Wisaniyasa Nico Kemit NN. Puspa wati Noval Wahyu Adi Noval Wahyu Adi Novita Aristha Anggreni Nainggolan Nyoman Geatrin Arumsari NYOMAN SEMADI ANTARA Paramita Wulandari, Kadek Ratih Praniti Radya Andana Ilma Puspasecca Wya Saraswati Putu Ari Sandhi Wipradnyadewi Putu Eka Yudhayanti Putu Risma Dewi Putu Vinanta Agus Putra Rina Pratiwi Pudja I. A Sandhi Wipradnyadewi, Putu Ari Sayi Hatiningsih Sumiyati Sumiyati Sutriswati Rahayu, Endang Tadashu Urashima Trisna Darmayanti , Luh Putu Virga Dewi Mahayani, Gusti Ayu Wayan Redi Aryanta Wayan Tika Wulandari Setiadarma Wulandari, Kristina Yan Ramona Yemima Dwi Gita Sembiring Yohanna Mei Sarah Purba Yoshitake Orikasa Yuji Oda