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Sex Determination of Sea Cucumber Acaudina rosettis from Madura Straits, Indonesia Winarni, Dwi; Lestari, Lita I.D.; Hayati, Alfiah; Nabilla, Nithasya; Khaleyla, Firas
Jurnal Riset Biologi dan Aplikasinya Vol. 4 No. 2 (2022)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v4n2.p83-89

Acaudina rosettis is one of the commercial sea cucumber species that is widely consumed on the east coast of Surabaya, East Java, Indonesia. Although its abundance in nature has begun to show signs of decreasing, studies on this species are still very limited. This study aimed to examine indicators that can be used to determine the sex of A. rosettis individuals. Acaudina rosettis samples were collected from Madura strait in the mid of Hijri month for three months. The sea cucumber samples were then dissected. Body wall without viscera and gonads was weighed. The gonads were then characterized macroscopically and microscopically, prepared into histological slides with hematoxylin-eosin staining. The results showed that A. rosettis samples could be classified into three sex groups based on the type of gamete cells observed in the tubules; male (spermatogenic), female (oogenic), and undetermined sex (no gamete cells found). Gonads had three colors; yellow, pink, and orange and were in five stages of reproductive phase; gametogenesis (G), mature (M), spawning (S), and post-spawning (PS). Male samples had yellow and orange gonads, female samples were pink and orange, and all undetermined sex were orange. The G gonads were yellow and orange, M were yellow and pink, while S and PS were orange. Body wall and gonad weight at the same reproductive phase did not show any difference between males and females. Based on the results, gonad color can be used as an indicator of sex determination, but only in G and M reproductive phases.

Pelatihan Tutor Sebaya sebagai Strategi Penguatan Pengetahuan Kesehatan Reproduksi Remaja pada Siswa SMP Labschool Unesa Nur Ducha; Dyah Hariani; Nur Kuswanti; Erlix Rakhmad Purnama; Firas Khaleyla; Prastyaningtias, Sisca Desi
BAKTIMU : Jurnal Pengabdian Kepada Masyarakat Vol 5 No 3 (2025)
Publisher : Universitas Muhammadiyah Ahmad Dahlan Cirebon

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37874/bm.v5i3.1792

Remaja termasuk kelompok usia yang rentan terhadap berbagai masalah kesehatan reproduksi akibat minimnya pengetahuan, pengaruh lingkungan, dan mudahnya akses informasi yang tidak sehat. Edukasi kesehatan reproduksi sejak dini sangat penting untuk mencegah perilaku berisiko seperti seks pranikah, pernikahan dini, serta infeksi menular seksual. Salah satu strategi yang efektif adalah pendekatan tutor sebaya yang menekankan pada peran siswa sebagai agen edukasi bagi teman sebayanya. Kegiatan pengabdian masyarakat ini bertujuan meningkatkan pemahaman dan keterampilan siswa SMP Labschool Unesa mengenai kesehatan reproduksi melalui pelatihan tutor sebaya. Pelaksanaan kegiatan meliputi tahap pelatihan (pemberian materi dan pretest) serta tahap implementasi (praktik tutor sebaya melalui pembuatan poster dan posttest). Hasilnya menunjukkan peningkatan pemahaman siswa sebesar 75% serta respon positif terhadap materi, metode, dan fasilitator. Kegiatan ini membuktikan bahwa pendekatan tutor sebaya efektif sebagai metode edukasi kesehatan reproduksi remaja, sekaligus dapat direplikasi pada sekolah lain.

Pelatihan Tutor Sebaya sebagai Strategi Penguatan Pengetahuan Kesehatan Reproduksi Remaja pada Siswa SMP Labschool Unesa Nur Ducha; Dyah Hariani; Nur Kuswanti; Erlix Rakhmad Purnama; Firas Khaleyla; Prastyaningtias, Sisca Desi
BAKTIMU : Jurnal Pengabdian Kepada Masyarakat Vol 5 No 3 (2025)
Publisher : Universitas Muhammadiyah Ahmad Dahlan Cirebon

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37874/bm.v5i3.1792

Remaja termasuk kelompok usia yang rentan terhadap berbagai masalah kesehatan reproduksi akibat minimnya pengetahuan, pengaruh lingkungan, dan mudahnya akses informasi yang tidak sehat. Edukasi kesehatan reproduksi sejak dini sangat penting untuk mencegah perilaku berisiko seperti seks pranikah, pernikahan dini, serta infeksi menular seksual. Salah satu strategi yang efektif adalah pendekatan tutor sebaya yang menekankan pada peran siswa sebagai agen edukasi bagi teman sebayanya. Kegiatan pengabdian masyarakat ini bertujuan meningkatkan pemahaman dan keterampilan siswa SMP Labschool Unesa mengenai kesehatan reproduksi melalui pelatihan tutor sebaya. Pelaksanaan kegiatan meliputi tahap pelatihan (pemberian materi dan pretest) serta tahap implementasi (praktik tutor sebaya melalui pembuatan poster dan posttest). Hasilnya menunjukkan peningkatan pemahaman siswa sebesar 75% serta respon positif terhadap materi, metode, dan fasilitator. Kegiatan ini membuktikan bahwa pendekatan tutor sebaya efektif sebagai metode edukasi kesehatan reproduksi remaja, sekaligus dapat direplikasi pada sekolah lain.

Lactic Acid Bacteria (LAB) from Fermented Milk Leftover for Wastewater Treatment of Soybean-Based Industries Trimulyono, Guntur; Isnawati; Asri, Mahanani Tri; Khaleyla, Firas
International Journal of Applied Biology Vol. 7 No. 1 (2023): International Journal of Applied Biology
Publisher : Hasanuddin University

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Tempeh and tofu are widely consumed by Indonesians and easily made using simple tools and method, thus numerous home industries producing are emerging. However, soybean processing in these industries produces high volume of wastewater. This study provides a simple wastewater treatment instrument design that could be used by soybean-based home industries by applying a series of physical, chemical, and biological treatment. Physical treatment was performed by filtration, chemical by addition of alum salts, while biological treatment was done by supplementing with lactic acid bacteria (LAB) from leftover container of fermented milk. Wastewater was recorded by its parameters (temperature, pH, dissolved oxygen/DO, biological oxygen demand/BOD, turbidity) at every phase of treatment after incubation for two various of periods; 1 hour and 5 days. Results showed that wastewater treated using instrument design had changes in all of its parameters observed during the course of treatment. Wastewater treated in all phase of instrument incubated for 1 hour showed parameters approaching wastewater quality standards, thus it was safer to be disposed to environment compared to wastewater incubated for 5 days. Wastewater treatment as designed in the current study can be applied by soybean-based home industries with short incubation time to remediate wastewater produced during soybean processing before disposal.

Morphological and Molecular Characterization of Pufferfish from Lekok Coastal Area, Pasuruan Indonesia Panjaitan, Viona Angelina Erlan; Rangga, Adam Dwi; Sulung, Cahya Ajeng Valenta Tresna; Rahayu, Dwi Anggorowati; Khaleyla, Firas; Nugroho, Endik Deni; Rusdianto, Rusdianto; Mamat, Noorhidayah Binti
Biosaintifika: Journal of Biology & Biology Education Vol. 17 No. 3 (2025): November 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v17i3.26663

Pufferfish belong to the Tetraodontidae family, which consists of 28 genera and 184 species; however, there are limited studies on the species from East Java, especially Pasuruan. This indicates the need for the collection of more accurate morphological and genetic resources as an important step for its conservation. Therefore, this study was conducted to identify pufferfish species found in the Lekok Coastal Waters, Pasuruan District, through morphological characterization and molecular analysis using DNA barcoding of the COI gene. A total of 12 samples were collected from the Lekok Coastal Waters, Pasuruan Regency. Fin samples were stored in 96% absolute ethanol. DNA extraction, polymerase chain reaction (PCR), and sequence analysis were performed using bioinformatics tools, the BOLD system, and the Automatic Barcode Gap Discovery (ABGD) web platform. Phenetic taxonomy was further applied using Ntysc to enhance the robustness of genetic analysis. The results showed that there were identified five pufferfish species were identified: Lagocephalus spadiceus, Chelonodontops patoca, Cyclichthys orbicularis, Arothron stellatus, and Arothron reticularis. High haplotype diversity (Hd) of 1 and nucleotide diversity (π) of 0.1 indicate significant genetic variation. The frequency of parsimonious informative sites was 22.6%, with 13 polymorphic sites and an overall ts/tv ratio of 2.2. The phylogenetic tree showed unambiguous branching patterns among species. These findings are supported by morphological and molecular identification results, which indicate the presence of five species of pufferfish along the Lekok Coast, Pasuruan Regency.

Pelatihan Pengolahan Limbah Kulit Kopi untuk Pakan Ternak Ruminansia di Desa Ngembat, Gondang, Mojokerto Khaleyla, Firas; Kuswanti, Nur; Qomariyah, Nur; Purnama, Erlix Rakhmad
Mitra Mahajana: Jurnal Pengabdian Masyarakat Vol. 4 No. 3 (2023): Volume 4 Nomor 3 Tahun 2023
Publisher : LPPM Universitas Flores

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37478/mahajana.v4i3.3135

Coffee is one of Indonesia's leading national commodities. However, coffee cultivation produces organic agricultural waste in the form of coffee husks. Based on its content, coffee husk waste still has the potential to be processed into other useful products, one of which is animal feed. The occupation of Ngembat village apart from cultivating coffee, are also raising ruminants, so the solution offered wasto carry out training on making animal feed with coffee husk waste. Community service activity was carried out on July 29, 2023 at the Ngembat Village Hall. The activity was attended by 17 residents of Ngembat village. The activity began with material regarding basic nutritional needs for ruminants and the content of coffee skins, then continued with participants practicing coffee husk fermentation. Coffee skin waste was fermented with Trichoderma sp. and the resulting product was evaluated after 5 days. The activity had received positive response from all participants and the product results of the participants had been as expected. With the implementation of training on making animal feed from coffee skin waste, it is hoped that it can reduce agricultural waste resulting from coffee cultivation carried out by the people of Ngembat village.

Pelatihan Tutor Sebaya sebagai Strategi Penguatan Pengetahuan Kesehatan Reproduksi Remaja pada Siswa SMP Labschool Unesa Ducha, Nur; Hariani, Dyah; Kuswanti, Nur; Rakhmad Purnama, Erlix; Khaleyla, Firas; Prastyaningtias, Sisca Desi
J-Dinamika : Jurnal Pengabdian Masyarakat Vol 10 No 3 (2025): J-Dinamika
Publisher : Politeknik Negeri Jember

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Remaja termasuk kelompok usia yang rentan terhadap berbagai masalah kesehatan reproduksi akibat minimnya pengetahuan, pengaruh lingkungan, dan mudahnya akses informasi yang tidak sehat. Edukasi kesehatan reproduksi sejak dini sangat penting untuk mencegah perilaku berisiko seperti seks pranikah, pernikahan dini, serta infeksi menular seksual. Salah satu strategi yang efektif adalah pendekatan tutor sebaya yang menekankan pada peran siswa sebagai agen edukasi bagi teman sebayanya. Kegiatan pengabdian masyarakat ini bertujuan meningkatkan pemahaman dan keterampilan siswa SMP Labschool Unesa mengenai kesehatan reproduksi melalui pelatihan tutor sebaya. Pelaksanaan kegiatan meliputi tahap pelatihan (pemberian materi dan pretest) serta tahap implementasi (praktik tutor sebaya melalui pembuatan poster dan posttest). Hasilnya menunjukkan peningkatan pemahaman siswa sebesar 75% serta respon positif terhadap materi, metode, dan fasilitator. Kegiatan ini membuktikan bahwa pendekatan tutor sebaya efektif sebagai metode edukasi kesehatan reproduksi remaja, sekaligus dapat direplikasi pada sekolah lain.

Pengembangan E-Book Interaktif Materi Sistem Pencernaan Manusia Untuk Melatih Keterampilan Berpikir Kritis Peserta Didik Kelas XI SMA wahyu ningtias, erika; Kuswanti, Nur; Khaleyla, Firas
Berkala Ilmiah Pendidikan Biologi (BioEdu) Vol. 15 No. 1 (2026)
Publisher : Program Studi Pendidikan Biologi, FMIPA, Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/bioedu.v15n1.p153-165

Pendidikan abad 21 meningkatkan perkembangan teknologi, penelitian dan ilmu pengetahuan di Indonesia. Sehingga peserta didik diharapkan memiliki keterampilan inovasi dalam pembelajaran, keterampilan berpikir kritis dalam memecahkan masalah, keterampilan penggunaan teknologi dan media informasi sehingga bermanfaat dalam life and career skills. Salah satu materi biologi yang cocok diterapkan dalam melatihkan keterampilan berpikir kritis adalah sistem pencernaan manusia. Penelitian memiliki tujuan guna menghasilkan e-book interaktif sistem pencernaan yang valid dan praktis untuk melatihkan keterampilan berpikir kritis. Pengembangan e-book menggunakan model 4D tanpa disseminate. Metode penelitian menggunakan lembar validasi dan respon. Validitas diukur bedasarkan validasi ahli materi, ahli pendidikan serta guru biologi SMA. Kepraktisan diukur berdasarkan lembar respon peserta didik terkait kemudahan penggunaan e-book. Uji coba terbatas dilakukan terhadap 20 peserta didik kelas XI IPA MA Unggulan Al-Falah Sidoarjo. Data dilakukan analisis secara deskriptif kuantitatif. Hasil penelitian yang diperoleh yaitu e-book interaktif tergolong dalam kategori sangat valid dengan skor mencapai 3,93. Kepraktisan mencapai 97,4% termasuk dalam kriteria sangat praktis. Dapat disimpulkan bahwa e-book interaktif sangat valid serta praktis untuk dapat diterapkan dalam pembelajaran sebagai media pada materi sistem pencernaan manusia secara mandiri maupun di dalam kelas.

SIMPLE EXPERIMENTS TO IMPROVE CHILDREN'S SCIENCE PROCESS SKILLS GUIDED PENANG LEARNING STUDIO INDONESIAN SOCIETY ORGANIZATION (PERMAI) Khaleyla, Firas; Yuliani, Yuliani; Indana, Sifak; Susantini, Endang; Raharjo, Raharjo; Kuswanti, Nur
Mitra Mahajana: Jurnal Pengabdian Masyarakat Vol. 7 No. 1 (2026): Volume 7 Nomor 1 Maret 2026
Publisher : LPPM Universitas Flores

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37478/mahajana.v7i1.6946

The Indonesian Community Association (PERMAI) is a nonprofit organization that supports Indonesian communities living in Malaysia. One of its educational programs is Sanggar Bimbing (SB) Permai, which provides access to education for Indonesian children who face difficulties obtaining formal education. In this program, students are introduced to Indonesian curriculum materials, including citizenship, history, culture, and the Indonesian language, as well as academic subjects such as basic science. However, limited facilities and teaching staff restrict the amount of material students receive. Therefore, an enrichment program on basic science was conducted through simple experiments to improve students’ science process skills. The program was implemented in three stages: preparation, implementation, and evaluation. The results showed a moderate improvement in students’ science understanding (N-gain = 0.53), very positive participant responses (average score = 4.38), and well-developed science skills, including observing, planning experiments, using tools and materials, and conducting experiments. Overall, the simple experiment activities received positive responses, although improvements are still needed to enhance their effectiveness and implementation according to students’ conditions.

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