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The potency of green grass jelly extract (Premna oblongifolia Merr) as antihyperlipidemia towards aorta histopathology representation of rat (Rattus norvegicus) induced with high fatty diet (HFD) Winthoko, Eka Nora Vitaloka Aprilia Putri; Roosdiana, Anna; Pratama, Dyah Ayu Oktaviani A; Nugraha, Jusak; Purwanta, Marijam; Rifa’I, Muh Husni; Rendy, Achmad Nur
Jurnal Teknologi Laboratorium Vol 9 No 1 (2020): 2020 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29238/teknolabjournal.v9i1.174

Abstract

Green grass jelly (Premna oblongifolia merr) is a plant containing fiber and chlorophyll which can lower cholesterol and triglyceride levels. This study have aims to investigate the potency of green grass jelly extract (Premna oblongifolia Merr) to prevent hyperlipidemia. The animal mode used for this study is male Rattus norvegicus, Wistar strain, the age of 8 weeks, and weight of 200 g which is divided into 5 groups of treatment namely group Kn (negative control), Kp (positive control), Kp1, Kp2, and Kp3 induced with HFD and green grass jelly extract at a dose of 5.27 g/ kg BW/ daily, 8.43 g/ kg BW/ daily, 9.37 g/ kg BW/ daily. The data of infiltrating fatty cells and macrophage on aorta histopathology was analyzed by description. This research showed that the treatment of green grass jelly extract (Premna oblongifolia Merr) to animals of hyperlipidemia model reduced infiltration fatty cells and macrophage. The conclusion of this study was the green grass jelly extract was able to prevent an increase of fatty cells and macrophage infiltration of rat (Rattus noervegicus) induced with HFD on dose 9.37 g/ kg/ BW/ daily.
INTERFERON GAMMA AND INTERLEUKIN-10 LEVELS IN PBMC OF ACTIVE AND LATENT TUBERCULOSIS PATIENTS AS WELL AS HEALTHY INDIVIDUALS Tedja, I G.A. Wiradari; Nugraha, Jusak; Tambunan, Betty Agustina; Oetami, Fransisca Sri
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 24, No 2 (2018)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v24i2.1309

Abstract

Tuberculosis (TB), an infectious disease caused by a Mycobacterium tuberculosis, is still a health problem in Indonesia, and the world. One of the failures to control the TB epidemic is due to the lack of effective vaccines available today. Protective immune responses to M.tuberculosis are dominated by cellular immunity and less by humoral immunity. IFN-γ, and IL-10 play a role in the protection of against M.tuberculosis, and the pathogenesis of TB. Fusion antigen ESAT-6-CFP-10 has a strong antigenicity to T cells and stimulates specific cellular immune responses, thereby providing benefit in immune responses that are protective against M.tuberculosis infection. The aimed of this study was to know the difference betwen IFN-γ, and IL-10 levels on PBMC culture of active TB, latent TB, and healthy people after ESAT-6-CFP-10 fusion antigen stimulation. This study used an in vitro of quasi experimental design in PBMC cultures of active TB, latent TB, and healthy people groups stimulated by ESAT-6-CFP-10 antigen fusion Mycobacterium tuberculosis. IFN-γ, and IL-10 levels were measured by ELISA method. The results were analyzed by one-way ANOVA. The mean levels of IFN-γ post-stimulation of ESAT-6-CFP-10 fusion antigens did not differ (p=0.359) in the active pulmonary TB group (0.07 - 2114), latent TB (6.84 - 1381) and healthy people 1.88 - 1807.70), as well as the mean levels of IL-10 (p=0.712) in the active pulmonary TB (16.70 - 328.80), latent TB (29.70 - 323.60 ) and healthy people (31.30 - 958). There were no significant differences in levels of IFN-γ and IL-10 in active TB, latent TB, and healthy people after stimulation by fusion antigen ESAT-6-CFP-10. 
Sekresi IFN-γ dan IL-10 Setelah Stimulasi Antigen Fusi ESAT-6-CFP-10 (EC610) pada Penderita TB Aktif dan TB Laten Sabrina Prihantika; Nova Kurniati; Kemas Ya’kub Rahadiyanto; M. Irsan Saleh; Zen Hafy; Francisca Srioetami Tanoerahardjo; Jusak Nugraha; Eddy Mart Salim
Biomedical Journal of Indonesia Vol. 5 No. 3 (2019): Biomedical Journal of Indonesia
Publisher : Fakultas Kedokteran Universitas Sriwijaya (Faculty of Medicine, Universitas Sriwijaya) Indonesia

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Abstract

Sistem imunitas seluler sangat berperan dalam melawan infeksi TB yaitu peran sel limfosit T yang berdiferensiasimenjadi sel Th1 mensekresikan sitokin pro inflamasi IFN-γ dan sel Th2 yang mensekresi sitokin anti inflamasi IL-10.Antigen fusi EC610 bersifat spesifik dan memiliki antigenitas yang kuat terhadap stimulasi sel T. Tujuan penelitian untukmengetahui perbedaan rerata kadar IFN-γ dan IL-10 setelah stimulasi antigen EC610 pada penderita TB aktif dan TBlaten. Desain penelitian ini adalah eksperimen semu secara in vitro dengan kultur PBMC yang distimulasi oleh antigenEC610 pada kelompok TB aktif dan TB laten. Penelitian dan pemeriksaan dilakukan di RSK Paru Provinsi Sumsel danLaboratorium Pusat Penelitian dan Pengembangan Kesehatan, Jakarta Pusat. Terdapat 21 subjek penderita TB aktif dan28 subjek penderita TB laten yang sesuai dengan kriteria inklusi. Pengukuran kadar IFN-γ dan IL-10 dilakukanmenggunakan ELISA-Reader. Hasil penelitian menunjukkan kadar IFN-γ dan IL-10 setelah stimulasi antigen EC610lebih tinggi pada TB aktif daripada TB laten. Tingginya kadar IFN-γ pada penderita TB aktif menunjukkan adanyarespon imun protektif terhadap kuman M.tb sedangkan tingginya kadar IL-10 menunjukkan perannya sebagai antiinflamasi . Tidak terdapat perbedaan bermakna rerata kadar IFN-γ pada penderita TB aktif dan TB laten (p=0,769) danterdapat perbedaan bermakna reata kadar IL-10 setelah stimulasi antigen EC610 lebih tinggi pada TB aktif daripada TBlaten (p=0,000).
IFN-γ and IL-2 Secretion after ESAT-6-CFP-10 (EC-610) Fusion Antigen Stimulation from Patients with Active Lung Tuberculosis and Latent Lung Tuberculosis Bastian; Nova Kurniati; Kemas Ya’kub Rahadiyanto; M. Irsan Saleh; Zen Hafy; Francisca Srioetami Tanoerahardjo; Jusak Nugraha; Eddy Mart Salim
Biomedical Journal of Indonesia Vol. 6 No. 2 (2020): Biomedical Journal of Indonesia
Publisher : Fakultas Kedokteran Universitas Sriwijaya (Faculty of Medicine, Universitas Sriwijaya) Indonesia

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Abstract

The Secretion of IFN-γ and IL-2 After ESAT-6-CFP-10 Fusion Antigen Stimulation in Activeand Latent TB Patients. This study held to discover how immune responses work and to know thepathogenesis of active TB and latent TB patients. This study used PBMC to stimulate T Cells withESAT-6 and CFP-10 antigen fusion, and measure the level of IFN-γ and IL-2 with ELISA antibodysandwich (U-Cytech). 16 ml of blood were drawn to 5 tubes. ESAT-6 CFP-20 inducted one tube withQuantiFERON for IFN-γ assay. The other four tubes were PBMC isolated using Ficoll-Paque, andpre-incubated with stimulation of ESAT-6 CFP-10 fusion antigen for 24-72 hours at 370 C andmeasured using T-Spot and ELISA reader. We got from this study that there are no significantdifferences in IFN-γ levels for both groups with active TB and latent TB. Measurement of IL-2 levelsshowed significant differences between the two group.
The Concordance of Dysmorphic Erythrocyte and Cast Erythrocyte Examination using Flowcitometry, Low Condenser Light Microscope, and Phase Contrast Microscope in Children with Glomerular Hematuria Maya E. Roring; Jusak Nugraha; Risky V. Prasetyo; Ferdy R. Marpaung
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 4 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i4.16964

Abstract

Homesickness, Anxiety and Depression among Pakistani International Students in Indonesia during Covid-19 Outbreak Shahzad Shoukat; Cyuzuzo Callixte; Theresia Indah Budhy; Jusak Nugraha; Tuyishimire Irene
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 4 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i4.17077

Abstract

Introduction: International students are exposed to multiple mental health crisis due to social, environmentaland culture shocks in foreign lands but it gets worse in presence of life- threatening disease outbreak. Thisresearch was conducted to evaluate the homesickness, anxiety and depression among Pakistani internationalstudents in Indonesia during Covid-19 Outbreak. Methods: This study was conducted on 86 randomstudents that are pursuing their studies in different public and private universities in Indonesia. The datawere collected by distributing google forms via email and social media groups and the responses fromthe respondents were recorded and analyzed statistically. Results: The study findings revealed that thereis a correlation between Covid-19 pandemic and the development of various mental health crisis where33 students (38.37 %) developed homesickness, 11 students (12.79%) developed anxiety and 2 students(2.33 %) manifested low-grade depression. Homesickness was outstandingly observed in females (72.72%) whereas anxiety was highly reported in males (90.90%).The study findings demonstrated that there isa strong negative correlation between student financial statuses and the development of homesickness (r:-0.977, P: 0.023) and anxiety (r: -0.944, P: 0.056).Conclusion: All in all, the fear to contract coronavirus,lockdowns, financial instability, death toll of citizens and medical professionals, run out of medical facilitiesand social media hoaxes are significant risk factors of mental health crisis among Pakistani internationalstudents in Indonesia.
Correlation Luteinizing Hormone (LH)/Follicle Stimulating Hormone (FSH) Ratio with Low Density Lipoprotein (LDL)/ High Density Lipoprotein (HDL) Ratio in Polycystic Ovarium Syndrome (PCOS) Patients Susi Oktaviani; Jusak Nugraha; Ashon Sa’adi
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 4 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i4.17106

Abstract

The aim of this study is to analyze the correlation between the ratio of LH and FSH levels with LDL andHDL cholesterol in PCOS patients. This study is an observational cross-sectional study. The population inthis study were women newly diagnosed with polycystic ovary syndrome who came to RSIA Ferina, RSIALombok 22, Elizabeth Clinic and RSIA Putri Surabaya and Clinical Pathology Laboratory of dr. SoetomoSurabaya. Examination of FSH, LH and LDL and HDL cholesterol levels on 27 specimens (stored frozenserum) using the ICT (Accurate Intan Madya®, VEDALAB) and enzymatic colorimetric (Dimension EXL®,Siemens) methods. Statistical analysis was performed using the Spearman and Pearson correlation test,with a significance level of p <0.05. The results showed that there was a moderate positive correlationbetween FSH and LDL cholesterol (r = 0.491, p = 0.009). Phenotype D of PCOS (non-hyperandrogenictype) had a moderate negative correlation between the LH / FSH ratio and the LDL/HDL cholesterol levelratio (r = -0.487, p <0.05). However, there was no relationship between the LH/FSH ratio and the ratioof LDL/HDL cholesterol levels in all SOPK patients in this study. This study showed that phenotype DPCOS patients (non-hyperandrogenic type) were less likely to have cardiovascular complications than otherphenotypic PCOS patients. Insulin resistance has a role in the risk of dyslipidemia in non-hyperandrogenicPCOS patients.
Qualitative Analysis of Cinnamomum burmannii Content using GCMS (Gas Chromatography Mass Spectometry) Method Hayati; Jusak Nugraha; Bambang Purwanto; Hari Basuki Notobroto; Yoes Prijatna Dachlan; Hari Setiono; Idha Kusumawati
Indian Journal of Forensic Medicine & Toxicology Vol. 16 No. 1 (2022): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v16i1.17556

Abstract

To identify the bioactive compounds in Cinnamomum burmannii and their biological activity. Cinnamonbark originating from Batu Malang, East Java, which was located 700-1300 meters above sea level, wasprocessed into dry extract by maceration method with 96% ethanol solvent. Furthermore, cinnamonethanol extract was analyzed using the GCMS method to look at the content of the bioactive componentfor further testing biological activity with the server Way2Drug PASS. GCMS results showed 40 activecompounds such as trans-cinnamaldehyde, trans-anethole, cinnamyl acetate, calacorene, cadina-1,4-diene, delta-cadinene. Furthermore, of the 40 compounds, the biological activity potential was testedfor 29 bioactive compounds based on PA (probable to be active) values predicted by the Way2DrugPASS server. Cinnamomum was tested against the potential as anti-fungi, anti-bacterial, anti-oxidant,anti-inflammatory, anti-diabetic, anti-neoplastic. Trans-cinnamaldehyde showed PA 0,583 as antiinflammatory, L-limonene PA0,818 as anti neoplastic, Tans-anethole PA 0,614 as anti neoplastic,Cinnamyl acetate PA 0,669 as anti inflammatory, calacorene PA 0,698 as anti inflammatory, DeltacadinenePA 0,651 as anti neoplastic, , Cathechin PA 0,828 as anti oxidant, alpha.-Cubebene PA 0,888 asanti inflammatory and PA 0,837 as anti neoplastic, melilotin PA 0,929 as anti neoplastic, CaryophyllenePA0,915 as anti neoplastic. Cinnamomum burmannii had biological potency based on potential activity(PA) 0,432 (+0,117) as antifungi, PA 0,335 (+0,090) as antibacterial, PA 0,304 (+0,199) as antioxidan,PA 0,561 (+0,190) as anti inflammatory, PA 0,373 (+0,170) as antidiabetic, PA 0,584 (+0,234) asantineoplastic.
Homesickness, Anxiety and Depression among Pakistani International Students in Indonesia during Covid-19 Outbreak Shoukat, Shahzad; Callixte, Cyuzuzo; Nugraha, Jusak; Budy, Theresia Indah; Shoukat, Hamad
Jurnal Kesehatan Masyarakat Vol 17, No 2 (2021)
Publisher : Department of Public Health, Faculty of Sport Science, Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/kemas.v17i2.31300

Abstract

International students are exposed to multiple mental health crisis due to social, environmental and culture shocks in foreign lands but it gets worse in presence of life- threatening disease outbreak. This research was conducted to evaluate the homesickness, anxiety and depression among Pakistani international students in Indonesia during Covid-19 Outbreak. Methods: This study was conducted on 86 random students that are pursuing their studies in different public and private universities in Indonesia. The data were collected by distributing google forms via email and social media groups and the responses from the respondents were recorded and analyzed statistically. The study findings revealed that there is a correlation between Covid-19 pandemic and the development of various mental health crisis where 33 students (38.37 %) developed homesickness, 11 students (12.79%) developed anxiety and 2 students (2.33 %) manifested low-grade depression. Homesickness was outstandingly observed in females (72.72 %) whereas anxiety was highly reported in males (90.90%).  The study findings demonstrated that there is a strong negative correlation between student financial statuses and the development of homesickness (r: -0.977, P: 0.023) and anxiety (r: -0.944, P:  0.056). All in all, the fear to contract coronavirus, lockdowns, financial instability, death toll of citizens and medical professionals, run out of medical facilities and social media hoaxes are significant risk factors of mental health crisis among Pakistani international students in Indonesia.  
Green Tea Suppresses Serum TNF-? and TGF-?1 Levels In Mice Model of Systemic Lupus Erythematosus Herin Mawarti; Jusak Nugraha; Djoko Agus Purwanto; Joewono Soeroso
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14746

Abstract

Objective: This study aims to investigate the effects of green tea administration on TNF-a, Hsp70, andTGF-b1 levels in the systemic lupus erythematosus (SLE). Material and methods: A total of 32 micewill be divided into four groups (each 8 mice), namely the control group, the SLE group, the SLE groupwho were given green tea extract at a dose of 500 mg/kg body weight, and the SLE group who were givengreen tea extract at a dose of 1000 mg/kg body weight. Analysis of TNF-a, Hsp70, and TGF-b1 levels wascarried out using the enzyme-linked immunosorbent assay technique. Results: TNF-a and TGF-b1 levelswere significantly increased in the SLE group compared to the control group (p < 0.05). This increase canbe significantly reduced through the provision of green tea, even reaching levels comparable to the controlgroup (p > 0.05). Conclusions: It was concluded that green tea containing EGCG can suppress TNF-a andTGF-b1 in the SLE model. Thus, green tea can be an alternative in immunology modulation in SLE.
Co-Authors Abdul Hafid B Agung Dwi Wahyu W Agung Dwi Wahyu Widodo Agustiningrum, Indah Akhmad Setyo Rahman Alfia Andriyani Amellya Octifani aminuddin, mohammad Anak Agung Istri Sri Wiadnyani Andrianto Anita Budiarti Anna Roosdiana Annette d’Arqom, Annette Anton Yuntarso Aryati Aryati Aryati Aryati Ashon Sa'adi Ashon Sa’adi Ashon Sa’adi Audrey Gracelia Riwu Awalia Awalia Ayu Imamatun Nisa Bambang Purwanto Bastian Bastian Bastian Bastian Beatrix, Stephanie Besin, Valentinus Betty Agustina Betty Agustina Tambunan Buana, Dwi Candra Budi Prasetyo Budi Utomo Budiutari, Ni Nyoman Budy, Theresia Indah Callixte, Cyuzuzo Caroline Caroline Ch. Destri Wiwis Wijayanti Christina Destri Citra Indah Setyaningrum Cynthia Cynthia Ayu Permatasari Cynthia Ayu Permatasari Cynthia Cynthia Cynthia Cynthia Cyuzuzo Callixte Darmadi, Epriyanto T Dheasy Herawati Diyan Wahyu Kurniasari Djoko Agus Purwanto Dominicus Husada Dwi Aris Agung Nugrahaningsih Dwi Liliek Kusindarta Dwiyanti Puspitasari, Dwiyanti Eddy Mart Salim Eddy Mart Salim Eddy Mart Salim Eddy Mart Salim, Eddy Mart Edhi Rianto Elok Budi Retnani Elvan Dwi Widyadi Erfina Lim Erna R Tobing Erwin Astha Triyono Esthy Poespitaningtyas Febtarini Rahmawati Ferdy R. Marpaung Ferdy Royland Marpaung Foe, Kuncoro Francisca Srioetami Tanoerahardjo Francisca Srioetami Tanoerahardjo Fransisca Srioetami Tanoerahardjo Fransisca Srioetami Tanoerahardjo Fransiska Fransiska Fransiska Sri Oetami Gede Arie Wijaya Handayani, Luh Putu Trys Monika Hanik Badriyah Hidayati,* Mohammad Hasan Machfoed,* Kuntoro,** Soetojo,*** Budi Santoso,**** Suroto,***** Budi Utomo****** Hans Kristian Nugraha, Hans Kristian Hantoro Gunawan Hari Basuki Notobroto Hari Basuki Notobroto Hari Setiono Harianto Notopuro Hartono Kahar, Hartono Hendy Wijaya Herin Mawarti Heru Setiawan Hery Priyanto Hevi Wihadmadyatami Holland Lydia Marpaung Huda Shalahudin Darusman I Made Andika Bara Kusuma Idha Kusumawati Ilham Ilham Irvan Ipandi Januarti, Catur Ifda Joewono Soeroso John Wiwin Julious Julious julious, julious Kadeq Novita Prajawanti Kasiyati, Menik Kemas Ya'kub Rahadiyanto Kemas Ya'kub Rahadiyanto Kemas Ya’kub Rahadiyanto kurniati, nova Lisa Soegianto Lumempouw, Silvia Luminto, Dian Lutfi Dewanda Nugroho M. Aminuddin M. Aminuddin M. Irsan Saleh Mangestuti Agil Maya E. Roring Meiti Muljanti, Meiti Mohammad Aminuddin Mohammad Hasan Machfoed Muhammad Amin Muhammad Amminuddin Muhammad Hamdan Muhammad Hamdan Muhammad Irsan Saleh Muhammad Nazarudin Munawaroh Fitriah Munawaroh Fitriah Munawaroh Fitriah Myrza Elita Naesilla Naesilla Nico Jafet Ningrum, Emilna Mega Ninik Asmaningsih Soemyarso Notopuro, Paulus Budiono Nova Kurniati Nova Kurniati Nova Kurniati Noviana, Rachmitasari Nugraha, Hans K Nur Hayati Nur Lailatul Fadhilah Oetami, Fransisca Sri Oryza Chrisantia Oski Illiandri Patria Dewi Pande Pratama, Dyah Ayu Oktaviani A Prihantika S., Sabrina Pudji Lestari Purwanta, Marijam Purwoko, Agus Puspitasari, Yessy Putri, Indah Aprianti Rahadiyanto, Kemas Ya'Kub Rahajuningsih Dharma Rahajuningsih Dharma Rahayu Nofita Sari Rahma Indah Pratiwi Ravell Hansen Untono Rendy, Achmad Nur Retno Indrawati Rifa’I, Muh Husni Risky V. Prasetyo Rochmah Kurnijasanti Roedi Irawan Rusli, Musofa Ryzky Widi Atmaja S. Soedarsono S.Pd. M Kes I Ketut Sudiana . Sabri Prihantika Sabrina Prihantika Saleh, M Irsan Saputra, Angky Saputri, Agatha Efrad Senny Yesery Esar Setiawan, Lidwina Tri Kristanti Shahzad Shoukat Sheila Clarissa Shoukat, Hamad Shoukat, Shahzad SILMI MARIYA Silvia F. Lumempouw Silvia Francina Lumempouw Sinansari, Restry Siti Nur Husnul Yusmiati Siti Rahmawati Soedarsono Soedarsono Soedarsono Soedarsono Soelistijo, Soebagijo Adi SP Edijanto Srikanth Karnati Süleyman Ergün Suryawan, I Gde Rurus Susi Oktaviani Tanoehardjo, Francisca Srioetami Tanoehardjo, Francisca Srioetami Tanoerahardjo, Francisca Srioetami Tedja, I G.A. Wiradari Theresia Indah Budhy Theresia Indah Budhy Sulisetyawati Triagung Ruddy Tuyishimire Irene Utariani, Arie UUS SAEPULOH Wahyu Dewi Tamayanti, Wahyu Dewi Wahyu Setiani Wibowo Wahyu Setiani Wibowo Winthoko, Eka Nora Vitaloka Aprilia Putri Wulandari, Dian Novita Wuryanto Hadinugroho Yetti Hernaningsih Yoes Prijatna Dachlan Yohanes Adrian Kapri Negara Yuani Setiawati Yudy Tjahjono Yufita Ratnasari Wilianto Yulia Nadar Indrasari Zen Hafy