Article Per Year (5 Year)
p-Index From 2020 - 2025
1.182
P-Index
This Author published in this journals
All Journal Journal of Tropical Biodiversity and Biotechnology Indonesia Chimica Acta Indonesian Journal of Chemistry Indobiosains Molekul: Jurnal Ilmiah Kimia
Harningsih Karim
Department of Pharmacy, School of Pharmacy Yamasi Makassar, 90222, South Sulawesi
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Environmental Science Immunology & microbiology Materials Science & Nanotechnology

Published : 7 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 7 Documents
Search

 1 
Production, Characterization, and Toxicity Test of L-asparaginase from Vibrio alginolyticus Bacterial Symbiont of Green Algae Caulerpa lentillifera Kasturiasih, Ni Putu; Ahmad, Ahyar; Arfah, Rugaiyah A.; Khairunnur, Siti; Soekamto, Nunuk Hariani; Taba, Paulina; Hala, Yusafir; Karim, Harningsih
Molekul Vol 18 No 3 (2023)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2023.18.3.8153

Abstract

L-asparaginase is an enzyme that can be used as an anticancer therapeutic agent. This study aimed to optimize fermentation time for production, optimization, and to test the toxicity of L-asparaginase from Vibrio alginolyticus (V. alginolyticus) bacterial symbiont of green algae Caulerpa lentillifera (C. lentillifera). The activity of L-asparaginase enzyme assay and the toxicity test were done by using the Nessler method and the Brine Shrimp Lethality Test (BSLT) method respectively. The results show L-asparaginase with 66 hours of fermentation time indicates the highest enzyme activity. L-asparaginase mentioned has an optimum enzyme activity of 17.99 U/mL for 30 minutes of incubation time at 37 °C, and pH 7.5. The BSLT results show LC50 value of 17.83 μg/mL indicating the enzyme is bio-toxic with high-level toxicity, which can continue for cytotoxicity tests on cancer cells.
Bioactivity and Metabolites Compounds of Medicinal Plants Endophytic Fungi in Indonesia Sukmawaty, Eka; Karim, Abdul; dwyana, Zarsawaty; Natsir, Hasnah; Karim, Harningsih; Ahmad, Ahyar
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 2 (2024): June
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.79070

Abstract

Indonesia is rich in diversity of medicinal plants, vital in traditional medicine and the pharmaceutical industry. However, overharvesting, along with population growth, land use changes, deforestation, and climate change, endanger these plants. This review investigates the potential of endophytic endosymbiont as an alternative. These endosymbionts can synthesise bioactive compounds similar to those found in medicinal plants.  This study compiled data from various sources on endophytic fungi and their bioactivity. The review aims to categorise Indonesian medicinal plants, to identify their associated endophytic fungi from different plant parts, and to assess their bioactivity. The results revealed numerous medicinal plant families and a variety of endophytic fungi isolated from fruits, leaves, twigs, bark, roots, and rhizomes. These fungi exhibited bioactivities, including antioxidant, anticancer, antidiabetic, and antimicrobial effects, with metabolites such as alkaloids, flavonoids, peptides, phenols, polyketides, quinones, steroids, and terpenoids. Fusarium and Colletotrichum were the most common endophytic fungi found. Notably, the biological activity was consistent among endophytic fungi from various host organs, but variations were observed according to the host's geographical origin. This suggests that Indonesia's diverse geography influences metabolite production and activity. However, the same host plant may harbour different species in distinct organs. These findings indicate that endophytic fungi within medicinal plants represent a promising source of bioactive compounds for future Indonesian medicine production. Future research should explore metabolite compounds and bioactivity across different geographical regions. 
Synthesis and Characterization of Nickel Nanoparticles: Biological and Photocatalytic Properties Fadliah, Fadliah; Raya, Indah; Ahmad, Ahyar; Taba, Paulina; Burhanuddinnur, Muhammad; Gaffar, Maming; Putri, Tri Widayati; Nur, Arfiani; Abubakar, Andi Nur Fitriani; Munadi, Rachmin; Majid, Ahmad Fudhail; Pratama, Irham; Yasser, M.; Jarre, Sulistiani; Karim, Harningsih
Indonesian Journal of Chemistry Vol 25, No 1 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.90034

Abstract

The potential uses of ecologically benign nickel nanoparticle manufacturing in various sectors, such as biomedicine, energy storage, and catalysis, have garnered much interest. This paper covers green approaches to nickel nanoparticle manufacturing, which integrate natural substances as stabilizing and reducing agents with eco-friendly processes. Phytochemicals derived from bacteria, microorganisms, and plant leaf extracts can convert nickel ions (Ni2+) into nickel nanoparticles. Room temperature is used for the synthesis procedure, and neither dangerous compounds nor unusual reaction conditions are used. Using a variety of analytical methods, the resultant nickel nanoparticles were characterized. We also investigate the possibility of using the generated nickel nanoparticles as a cytotoxic, photocatalytic, antioxidant, and antibacterial agents. The antimicrobial activity of nickel nanoparticles demonstrates their potent antibacterial properties, while their antioxidant activity demonstrates their capacity to combat free radicals effectively. Furthermore, nickel nanoparticles' cytotoxic activity demonstrates their capacity to kill cancer cells, and their photocatalytic activity demonstrates their efficiency in breaking down organic contaminants. This review highlights the value of ecologically benign synthetic methods and creates new avenues for developing nickel nanoparticle applications in health and the environment.
A Review of Marine Bacterial Intracellular and Extracellular Bioactive Compounds as Novel Antibacterial and Anti-Inflammation Agents Karim, Harningsih; Azis, Arief; Ramadani, Ananda; Anita, Anita; Ahmad, Ahyar; Natsir, Hasnah; Taba, Paulina; Putri, Suriati Eka; Sarlan, Sarlan; Larekeng, Siti Halimah; Irfandi, Rizal
Indonesian Journal of Chemistry Vol 25, No 1 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.90039

Abstract

Unique and varied bioactive compounds produced by the ocean have drawn attention and served as a focus for creating antibacterial and anti-inflammatory agents. As part of the approach for locating these research sources, databases such as PubMed, Science Direct, MDPI, Google Scholar, Springer Link, Web of Science, Scopus, and Wiley Online Library were used to identify completed studies. Numerous intriguing bioactive compounds have so far been isolated from marine bacteria. A crucial resource in the ongoing search for novel peptides, proteins, lipids, nucleosides, enzymes, alkaloids, polyketides, and terpenoids is the diversity of marine bacterium strains. This review summarizes several bacterial intracellular and extracellular bioactive compounds that have been applied as antibacterial and anti-inflammatory agents in 2016–2024, which we present in the form of structures, species sources, and evaluations of these compounds' antibacterial and anti-inflammatory activities. Therefore, this review article can be used as a recommendation for utilizing biomaterials from marine bacteria that are promising in the future for industrial-scale production of antibacterial and anti-inflammatory agents.
The Utilization of Gracilaria sp. as Raw Material for Cellulose in Cellulose Acetate-Nickel Oxide (CA-NiO) as Electrode for Energy Storage Technology Karim, Abdul; Ahmad, Ahyar; Arfah, Rugaiyah Andi; Ermawar, Riksfardini Annisa; Karim, Harningsih; Putra, Andi Erwin Eka; Putri, Suriati Eka; Negara, Satria Putra Jaya; Larekeng, Siti Halimah
Indonesian Journal of Chemistry Vol 25, No 1 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.96533

Abstract

In the modern era, electrical energy has become a significant need that drives the large consumption of fossil fuels and their environmental impacts. Supercapacitors can reduce this large consumption of natural polymers such as cellulose acetate (CA), which can be synthesized from Gracilaria sp. Composites with CA can be an environmentally friendly alternative electrode with low toxicity. The results show that the cellulose has been successfully synthesized from the algae Gracilaria sp., which was proven by FTIR spectra analysis. The results also show that supercapacitor electrodes have been successfully made where the manufactured electrodes form a composite between CA and nickel oxide (NiO), with the highest specific capacitance and specific energy values of 15.5 × 102 F/g and 13.3 × 102 Wh/kg, respectively, on the CA-NiO2 electrode, but when the NiO concentration is higher than the CA concentration the specific capacitance and specific energy decrease as shown on the CA-NiO electrode with NiO mass of 0.6 g. Thus, the materials results of this study can be applied to electric vehicles and technology that requires electricity storage in the future.
Optimasi Pertumbuhan Bakteri Laut Penghasil Protease Menggunakan Variasi Konsentrasi Media Skim Milk Modifikasi Anita, Anita; Natsir, Hasnah; Ahmad, Ahyar; Taba, Paulina; Karim, Harningsih
Indobiosains 2025: Volume 7 Nomor 1 Februari 2025
Publisher : Universitas PGRI Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31851/indobiosains.v7i1.17822

Abstract

This study aims to analyze the growth rate of marine bacteria producing protease using variations in the concentration of skim milk media and to observe the relationship between cell growth and enzyme activity. The study was conducted using three concentrations of skim milk media (1%, 2%, and 3%) and observation of growth and enzyme activity for 108 hours. The results showed that 1% skim milk concentration produced the largest proteolytic zone diameter (13.8 mm) with the fastest growth (1 day). Observations of cell growth patterns and enzyme activity showed that the highest enzyme activity (0.5933 U/mL) occurred in the early growth phase and decreased in subsequent phases even though cell growth increased. This indicates that there is no linear relationship between cell growth and enzyme activity in marine bacteria-producing protease, where enzyme production and activity are influenced by various complex factors such as physiological conditions of the cells and nutrient availability.
The Toxicity and Cytotoxicity Test of Anticancer Peptides Isolated from Symbiotic Bacteria Asmi, Nur; Ahmad, Ahyar; Natsir, Hasnah; Karim, Harningsih; Muhakim, Ali; Khairunnur, Siti
Jurnal Akta Kimia Indonesia (Indonesia Chimica Acta) Vol. 18 No. 1 (2025): Volume 18, No 1: June 2025
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70561/ica.v18i1.43925

Abstract

The purpose of this research was to evaluate the toxicity and cytotoxicity of the fragment peptide from symbiotic bacteria in the lung cancer LK-2 cell line, compared normal M5S cell line. Protein hydrolyzates were separated using Molecular Weight Cut Off (MWCO) to isolate peptide fragments. Fragments were categorized as ≤ 5 kDa, > 5-10 kDa, and > 10 kDa to evaluate their toxicity through the Brine Shrimp Lethality Test (BSLT) method and to study cytotoxicity effects and proliferation in LK-2 lung cancer cells against normal M5S cells. MTT assay was used to analyze the percentage of living cells and cell growth. The Selectivity Index (SI) equation was applied to determine selectivity. Findings indicated that the peptide fragment ≤ 5 kDa had the highest toxicity, with an LC50 of 8.15 µg/ml. The number of LK-2 cells that died increased when exposed to peptide fragments of sizes ≤ 5 kDa, > 5-10 kDa, and > 10 kDa over 16 hours. Moreover, cell growth in LK-2 cells decreased after 16 hours, while there was no significant decline (P < 0.05) in M5S cells compared to the controls. The peptide fragments with molecular weights ≤ 5 kDa and > 5-10 kDa demonstrated the highest selectivity, resulting in an SI value of 3. These results suggest that the selected peptide fragments may serve effectively as anticancer agents. This study highlights the potential of peptides from symbiotic bacteria as possible treatments for cancer, particularly lung cancer.
Co-Authors Abdul Karim Abubakar, Andi Nur Fitriani Ahmad Fudhail  Majid Ahyar Ahmad Ali Muhakim Andi Erwin Eka Putra Anita Anita Arfah, Rugaiyah A. Arfah, Rugaiyah Andi Arfiani Nur Burhanuddinnur, Muhammad dwyana, Zarsawaty Fadliah, Fadliah Hasnah Natsir Indah Raya Irfandi, Rizal Kasturiasih, Ni Putu Khairunnur, Siti Maming Gaffar, Maming Munadi, Rachmin Negara, Satria Putra Jaya Nunuk Hariani Soekamto Nur Asmi Paulina Taba PRATAMA, IRHAM Putri, Tri Widayati Ramadani, Ananda Riksfardini Annisa ERMAWAR Sarlan, Sarlan SITI HALIMAH LAREKENG, SITI HALIMAH Sukmawaty, Eka Sulistiani Jarre Suriati Eka Putri Yasser, M Yusafir Hala