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Journal : Biocelebes

PERTUMBUHAN MISELIUM JAMUR TIRAM PUTIH (Pleurotus ostreatus) PADA MEDIA FORMULA JERAMI JAGUNG DAN LIMBAH BIJI KOPI umrah umrah; Eny Yuniati; Amiruddin Kasim; Kirana Kirana
Biocelebes Vol. 16 No. 1 (2022)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v16i1.15950

Abstract

Corn straw and coffee bean waste as a medium and source of nutrients needed for the growth of mycelium white oyster mushroom (P. ostreatus). This research has been carried out at the Microbiology Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Tadulako University. The purpose of this study was to determine the growth of mycelium and the characteristics of white oyster mushroom (P. ostreatus) in the formulation of corn straw and coffee bean waste media. This study was designed in a completely randomized design (CRD), consisting of seven treatments and three replications, namely P1 (100% corn straw powder), P2 (95% corn straw powder + 5% coffee bean waste), P3 (90% corn straw powder + 10% coffee bean waste), P4 (85% corn straw powder + 15% coffee bean waste), P5 (80% corn straw powder + 20% coffee bean waste), P6 (75% corn straw powder + 25% coffee bean waste), P7 (70% sawdust + 20% rice bran + 10% corn flour as a positive control). The results showed that corn straw and coffee waste which were used for growth media of white oyster mushroom mycelium (P.ostreatus) could be used as basic media because mycelium could grow well in all treatments. The growth rate of white oyster mushroom mycelium in P3 treatment enjoyed very fast growth and P6 treatment showed very slow growth. The highest number of colonies was in treatment P3 6.40x1011CFU/grand the lowest number of colonies was in treatment P6 2.66x1011CFU/g. Treatment P3 takes a shorter time to fill the culture bottles, which is 30 days, while P6 takes a longer time to fill the culture bottles, which is 48 days.
FORMULASI MEDIA PERTUMBUHAN MISELIUM JAMUR TIRAM PUTIH (Pleurotus ostreatus (Jacq.) P. Kumm.) BERBAHAN DASAR DEDAK PADI DAN LIMBAH KOPI Umrah, Umrah; Triana, Nina Risnanda; Yuniati, Eny; Kasim, Amiruddin; Zainal, Amalia Purnamasari
Biocelebes Vol. 18 No. 1 (2024)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v18i1.17198

Abstract

Rice bran and coffee waste as a medium and source of nutrients needed for the growth of the white oyster mushroom mycelium (Pleurotus ostreatus (Jacq.) Kumm.). This research has been carried out at Microbiology Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Tadulako University. This study has almed : (1) To determine the growth of white oyster mushrooms (P. ostreatus (Jacq.) Kumm.) in the formulation of rice bran and coffee waste media. (2) Knowing the ratio in the media formulation of rice bran waste and coffee waste that can provide the best growth of white oyster mushroom (P.ostreatus (Jacq.) Kumm.). Data analysis research used in a completely randomized design (CRD), consisting of seven treatments and three replications, namely P1 (100% rice bran), P2 (95% rice bran + 5% coffee waste powder), P3 (90% rice bran + 10% coffee waste powder), P4 (85% rice bran + 15% coffee waste powder), P5 (80% rice bran + 20% coffee waste powder), P6 (75% rice bran + 25% coffee waste powder), P7 (sawdust 70% + rice bran 20% + corn flour 10% (positive control). The results showed that the best mycelium of growth were P7 and P1. The highest number of colonies were at treatment P7 1,26x1012CFU/gr and the lowest was P1 0,26x1012CFU/gr. The fastest incubation time was found in P7, which was 25 days and the lowest was in treatment was P6, 48 days.