Article Per Year (5 Year)
p-Index From 2021 - 2026
3.593
P-Index
This Author published in this journals
All Journal ACTA VETERINARIA INDONESIANA Indonesian Journal of Electronics and Instrumentation Systems METAMORFOSA Journal of Biological Sciences Jurnal Ilmu Dasar AL KAUNIYAH BERKALA SAINSTEK Biogenesis: Jurnal Ilmiah Biologi UNEJ e-Proceeding Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) Universa Medicina Jurnal Bioteknologi & Biosains Indonesia (JBBI) BIOEDUKASI Warta Pengabdian Jurnal Biolokus: Jurnal Penelitian Pendidikan Biologi dan Biologi Bioma : Jurnal Biologi dan Pembelajaran Biologi Jurnal Riset Biologi dan Aplikasinya Life Science and Biotechnology Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia Berita Biologi
Rike Oktarianti
Jurusan Biologi FMIPA Universitas Jember, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Control & Systems Engineering Education Electrical & Electronics Engineering Environmental Science Health Professions Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Veterinary

Published : 40 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 40 Documents
Search

 1   2   3   4 
COLOR BLIND PREVALENCE OF ELEMENTARY STUDENT IN SUMBERSARI SUBDISTRICT, JEMBER Rike Oktarianti; Lutfia Azizah; Eva Tyas Utami
BIOEDUKASI Vol 20 No 1 (2022)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v20i1.31199

Abstract

Red-green color blindness is the most common type of color blindness. Color blindness disorders occur due to genetic factors determined by the recessive gene c (color blind) which is linked to the X chromosome. This study aims to determine the prevalence and frequency of color blind alleles and to determine the pattern of inheritance of color blind genes by conducting pedigree analysis on color blindness student’s family. The research was conducted in 13 public elementary schools, Sumbersari subdistrict, Jember Regency with a multistage cluster sampling method of sampling with a total sample of 286. Detection of color blindness using Ishihara's methods. Based on the results of this study, the color blindness prevalence of elementary school students in Sumbersari subdistrict, Jember was 2.79% with the prevalence in males and female were respectively as 4.86% and 0.7%. The allele frequency of color blindness was 0.021 and the normal allele frequency was 0.979. The pedigree analysis of a color blind student family shows the gene of color blindness is inherited in a criss-cross inheritance pattern.
USING sma-its2 PRIMER FOR ITS2 (Internal Transcribed Spacer-2)-BASED MOLECULAR CHARACTERIZATION OF Anopheles minimus FROM KULONPROGO, YOGYAKARTA - INDONESIA Syubbanul Wathon; Berlian Permata Dewi Erlambang; Naura Paramitha Cindy Ardyah; Devi Astikaningrum; Rike Oktarianti; Kartika Senjarini
BIOEDUKASI Vol 20 No 1 (2022)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v20i1.31911

Abstract

Malaria is a public health threat caused by the Plasmodium infection transmitted by the Anopheles mosquito. Malaria vector control is highly dependent on the ability to determine mosquito species' vectorial and bionomic capacity. Species identification based on morphological characteristics as well as DNA-barcoding approaches is a very important step in determining vectorial capacity. Our research group has redesigned a new ITS2 primer, namely, sma-its2, which is specifically able to identify the Anopheles (An.) mosquito vector but cannot identify other mosquito vectors. This study wanted to test this primer's specificity further for identification of other Anopheles mosquitoes. We used An. minimus collected from Kulonprogo, Yogyakarta – Indonesia. The methods used in this research are as follows: landing collection, morphological identification, isolation of genomic DNA, PCR (Polymerase Chain Reaction), PCR product purification, sequencing, and data analysis. An. vagus from Bangsring, Banyuwangi – Indonesia, which had previously been identified using the same primary, was used as a positive control. The results of the morphological analysis showed that both species were in accordance with the vector identification key used in this study. The molecular analysis showed that the sma-its2 primer could amplify the ITS2 sequence of An. vagus and An. minimus, producing 650 – 700 bp. However, further analysis of the ITS2 sequences of both species, resulted in the same species, namely An. vagus, with a different accession number in GenBank. This showed that the sma-its2 primar can be used to identify An. vagus but cannot be used to identify An. minimus. Analysis of the primer position in the ITS2 sequences showed the presence of 3 nucleotides in the forward sma-its2 primer that was not recognized by the An. minimus sequences and thus, hinder the successful identification of these species.
In silico study to predicting Apyrase-ADP binding affinity from salivary gland of Aedes aegypti in inhibition of platelet aggregation by molecular docking Rike Oktarianti; Silvya Fitri Nur Azizah; Ari Satia Nugraha; Syubbanul Wathon; Kartika Senjarini
Biogenesis: Jurnal Ilmiah Biologi Vol 10 No 2 (2022)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v10i2.33227

Abstract

.
ANOPHELES MICROBIOTA IN MALARIA VECTOR AND THE EFFECT ON PARASITE TRANSMISSION Dita Paramytha Agustin; Berlian Permata Dewi Erlambang; Cizein Tri Cahyanti; Syubbanul Waton; Rike Oktarianti; Kartika Senjarini
BIOEDUKASI Vol 19 No 2 (2021)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v19i2.25165

Abstract

Malaria is a vector-transmitted disease with a high number of cases. Plasmodium parasites are transmitted from the body of the Anopheles mosquito to the host through several mosquito organs, including the salivary glands and the midgut. The salivary glands and the midgut, apart from being a breeding ground for parasites, are known from several studies that various types of microbiota inhabit these two organs. The group of bacteria is the most widely known to be associated with Anopheles mosquitoes. The bacteria found were dominated by Gram-negative bacteria, with Pseudomonas being the most common, followed by Aeromonas, Asaia, Comamonas, Elizabethkingia, Enterobacter, Klebsiella, Pantoea, and Serratia. The group of Gram-positive bacteria was represented by the genera Bacillus, Streptococcus, Lactobacillus, and Staphylococcus. Each species of Anopheles has a different composition of associated bacteria. Anopheles-associated bacteria currently receive much attention because of their role in fighting Plasmodium infection. The ability of malaria vector-associated bacteria to inhibit and fight Plasmodium infection is divided into three mechanisms. Bacteria can triger an immune response through the Immune-deficiency (IMD) pathway, which causes active anti-Plasmodium and the production of antimicrobial peptides, increasing ROS synthesis and microbiota, which trigger hemocyte differentiation to protect against Plasmodium. This function related to inhibiting Plasmodium development needs further research so that later it can become an option for vector-based malaria control without damaging the sustainability of the environment.
Prevalensi Kemampuan Mengecap Phenylthiocarbamide (PTC) Pada Penduduk Pulau Gili Ketapang Kabupaten Probolinggo Rike Oktarianti; Siti Agustina Wulandari; Hidayat Teguh Wiyono; Mahriani Mahriani
Metamorfosa: Journal of Biological Sciences Vol 10 No 1 (2023)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2023.v10.i01.p07

Abstract

The taste of sensitivity of Phenylthiocarbamide (PTC), is autosomal dominant trait inherited. The taster controled by dominant allele while the non-taster determinated by recessive allele. The purpose of this study was to determine the prevalence and frequency of taster and non-taster alleles for PTC, as well as non-taster inheritance patterns at the population of Gili Ketapang island, Probolinggo district. Determination of the sample or respondents was carried out by randomly, total sample were 384 people, aged from 15-30 years. Detection of the taste sensitivity PTC was ask to respondents to taste the PTC solution. The PTC solution used was from the lowest concentration of 0.32mg/L (P13) to the highest concentration of 1300 mg/L (P1). The results showed that the prevalence of taster and non-taster were 96.88% and 3.12%, respectively. The frequency of taster (T) and non-taster alleles was 0.83 and non-taster was 0.17. The pedigree analysis of non-taster showed that non-taster individuals were born from heterozygous taster pairs (Tt) or from marriages between heterozygous taster individual (Tt) with non-tasters (tt). Keyword: Phenylthiocarbamide (PTC), taster, non taster
In silico exploration of the potential barcode DNA in Anopheles sp., a malarian vector from Indonesia Syubbanul Wathon; Devi Astikaningrum; Naura Paramitha Cindy Ardyah; Rike Oktarianti; Kartika Senjarini
Jurnal Biolokus Vol 6, No 1 (2023): June
Publisher : Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/biolokus.v6i1.1749

Abstract

Malaria is an infectious disease caused by Plasmodium and transmitted to humans through the Anopheles mosquito vector. The large diversity of Anopheles in Indonesia, which consists of similar complex species, makes it difficult to identify species based on morphological characteristics. Therefore, it is necessary to have another approach to identifying species based on molecular characteristics using DNA molecular markers. This approach becomes important as a basis for vector-based malaria control efforts. This research aims to explore potential DNA barcodes based on in silico studies as a reference for DNA barcoding analysis of Anopheles species in Indonesia. The data sequences of CO1 and ITS2 from Anopheles sp. were collected on the NCBI database. The sequence alignment was performed using CLUSTALX2. The construction of a phylogenetic tree was carried out using MEGA XI. Data analysis was performed by observing the construction of phylogenetic trees, bootstrap scores, and genetic distance. The data sequence in NCBI shows that 10 species of the genus Anopheles as malaria vectors have been studied in Indonesia. Sequence alignment of the 10 species based on CO1 and ITS2 markers showed that ITS2 markers had higher genetic variation than CO1. Therefore, the ITS2 sequence has the potential to be a DNA barcode for the molecular identification of Anopheles.
Relative Frequency (Composition) of Anopheles sp. Mosquitoes as Malaria Vectors in Kulon Progo District Special Region of Yogyakarta Wathon, Syubbanul; Senjarini, Kartika; Masruroh, Binti; Oktarianti, Rike; Rehmann, Holger
Jurnal Riset Biologi dan Aplikasinya Vol. 6 No. 1 (2024)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v6n1.p20-33

Abstract

Malaria is caused by Plasmodium infection transmitted Anopheles sp. One of the obstacles in malaria control is the variation of Anopheles sp. Species which have various characteristics and behaviour, so it’s very important to understand the species of Anopheles sp. with aim to develop the disease control program. This study was conducted in Kulon Progo Regency, Yogyakarta, one of the malaria endemic areas in Indonesia. Data collection of Anopheles sp. species diversity was carried out through landing collection inside and outside people’s homes at 18.00 - 06.00 WIB. Observations of abiotics factors in the habitat of Anopheles sp. larvae was carried out around the landing collection location. The result of research conducted in the Samigaluh, Kalibawang, and Pengasih Sub-Districts showed that the most common species found was Anopeheles vagus. The dominance of Anopheles vagus occurred in the Kalibawang and Pengasih dictricts, while in the Samigaluh Sub-Districts it became the second most among other species. Measurement of abiotic factors in the habitat of Anopheles sp. larvae through the parameters of temperature, air humidity, pH DO of water shows that the location where the larvae were found can support larval breeding and growth. That can be a factor in the large population of Anopheles sp., especially Anopheles vagus, which is a known vector of malarias based on research and its vectorial capacity.
Identification of Immmunogenic Salivary Proteins of Anopheles vagus based on Mass Spectrometry Analysis Febriyantiningsih, Dwi Esti; Senjarini, Kartika; Oktarianti, Rike
Jurnal ILMU DASAR Vol 18 No 2 (2017)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (702.853 KB) | DOI: 10.19184/jid.v18i2.3106

Abstract

Malaria has been prevalent for a long time in tropical developing regions causing great morbidity and mortality. Among the malaria vectors, Anopheles vagus has been known as secondary malaria vector in East Java. Salivary glands of mosquitoes perform various functions for survival of the vectors and also conducive for blood feeding, harbouring of malaria parasites, and eventual parasite transmission. The salivary gland proteomes of An. vagus have not been carried out yet. The aim of our study was to identify and characterize the immunogenic proteins of salivary glands proteins of An. vagus. A proteomic approach combining one-dimensional electrophoresis (1DE) followed by western blot analysis using human sera from healthy people living in an endemic area (Kendal); liquid chromatography mass spectrometry (LC-MS/MS) and bioinformatic analysis was adopted to provide the first direct insight into identification and characterization of salivary proteins of An. vagus. Identification of immunogenic proteins using western blot analysis has revealed three immunogenic bands which had molecular weights of 69, 75 and 232 kDa. Among those proteins analysed by LC-MS/MS, there were alpha,1-4 glucan phosphorylase, putative myosin class I heavy chain which have the highest number of total spectrum count peptide. Other proteins like vitellogenin and heat shock protein 82 (Hsp82) were also identified. The majority of proteins were scrutinized marked for their role in metabolism, cytoskeleton protein and stress response. Keywords: Anopheles vagus, salivary gland, immunogenic, proteomics
The Diversity of Potential Malaria and Dengue Mosquito Vector from Bangsring Village Wongsorejo District Banyuwangi East Java Azkiyah, Siti Fat'hiyatul; Senjarini, Kartika; Oktarianti, Rike; Wiyono, Hidayat Teguh; Wathon, Syubanul
Jurnal ILMU DASAR Vol 22 No 1 (2021)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v22i1.13601

Abstract

Bangsring village in Wongsorejo regency has been reported as malaria endemic area in Banyuwangi since 2011. Understanding the diversity and behavior of mosquito vector in this area will be very impotant in developing vector control program. The masquitoes were collected by landing collection outside and inside house by human bites, we also observed the area around cattle cage. During observation periods, a total 633 masquitos were collected consist of 44% malaria potential vector (Anopheles spp.) 19% Dengue fever (DF) potential vector (Aedes spp.) and 36% were not belong to both of them. Out of total collected Anopheles spp. mosquitoes about 65% were indentified as An. vagus, 25% were An. indevinitus, 8% were An. vagus (limosus), and only 2% as well as 0,04 % were An. supictus and An. kochi. Meanwhile we found 19% of Aedes sp. were Ae. aegypti and 81% were Ae. albopictus. This study showed that the dominan potential malaria’s vector is An. Vagus and the dominan DF vector is Ae. albopictus. Mostly colleted Anopheles sp. were exophagic and zoophilic with the highest activities between 06.00-08.00 pm. This was in contras to Aedes sp. where mosfly found endophilic and it has 2 hightime of activities Aedes sp. between 06.00- 07.00 AM and 05.00-06.00 PM. Keywords: Dengue, Malaria, diversity, Anopheles, Aedes.
Genetic Study of Phenylthiocarbamide (PTC) Taste Sensitivity In Population of The Osing in Kemiren Village-Banyuwangi Indriani, Fenny; Oktarianti, Rike; Wathon, Syubbanul
BERKALA SAINSTEK Vol 9 No 1 (2021)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v9i1.19844

Abstract

The ability to taste phenylthiocarbamide (PTC), is autosomal trait inherited in a simple Mendelian recessive pattern. The frequency of Taster and non-Taster allele is varies in different populations. The purpose of the research is to investigate the prevalence, gene frequency and genotype frequency of taster (T) and non taster (ts of Osing population in Kemiren-Banyuwangi. PTC serial dilution method was used to assess the PTC Taster and non-Taster phenotypes. The Hardy–Weinberg method was used to determine allele frequencies. The total of samples were 227 people, male were 117 and female were 110 with age range of 15–30 years were randomly selected. The result showed that the Osing population as Taster were 210 (92,52%) and non Taster were 17samples (7,48%) . The allele frecuency of Taster (T) was 0,73 and non Taster (t) was 0,27 respectively. The genotype frequency of dominant Taster (TT) was 0,54, heterozygosity Taster (Tt) was 0,39, and genotype of non Taster (tt) was 0,07.
Co-Authors Agustin, Dita Paramytha Ahmad Tosin Aida Mursyidah Ainiyah, Durotun Amania, Novita Aminuyati Anam, Khairul Ardyah, Naura Paramitha Cindy Ari Satia Nugraha Arofa, Resmining Mega Azkiyah, Siti Fat'hiyatul Berlian Permata Dewi Erlambang Cizein Tri Cahyanti Devi Astikaningrum Devi Astikaningrum Dewi Masruroh Dita Paramytha Agustin Eva Tyas Utami Febriyantiningsih, Dwi Esti Finasrullah, Aufar Fitria Mutiah Fitria Muti’ah Hidayat Teguh Wiyono Husnatun Nihayah Iflaha, Adriana Ihsanul Azmi Indriani, Fenny Intan Fitri Indrasari Kartika Senjarini Khasanah, Rochmatul Nuryu Kirana Eka Rezki Labes, Antje Laili, Alvina Nur Lailly Nur Uswatul Hasanah Lelono, Asmoro Lutfia Azizah Mahriani Mahriani Mamik Pristiwindari MAsruroh, Binti Maulana Jauharil Habib Miatin Alvin Septianasari Mochtar Gunawan Wibisono Muhammad Khalid Abdullah Mutiah, Fitria Nadya Rismana Fitriani Nailatur Rifdah Naura Paramitha Cindy Ardyah Naura Paramitha Cindy Ardyah Novita Amania Nur Aisyah Septiana Nuril Azizah Purwatiningsih Purwatiningsih Putra, Iguh Widigda Rahmawati, Itsna Ratis Nour Sholichah Rehmann, Holger Renam Putra Arifianto Rendy Setiawan Riana Agatha Listiani Saraswati, Dyah Rahmadhani Silvya Fitri Nur Azizah Siti Agustina Wulandari Sri Mumpuni Sri Mumpuni Wahyu Widajati, Sri Mumpuni Wahyu Syubbanul Wathon, Syubbanul Syubbanul Waton Syubhanul Wathon Tri Yudani MR Utami, Diah Ayu Wahyu Tri Agustin Wathon, Syubanul Wathon, Syubbanul - Widya Cahyadi Widyadana, Kaysha Rahmadita Wulandari, Ayu Dwi Yasir Mubarok Yenny Febriana Ramadhan Abdi Zahniar, Zahniar