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Oom Komala
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Journal : BERITA BIOLOGI

 1 
UJI EFEKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAN EKSTRAK AIR KELOPAK BUNGA ROSELLA (HIBISCUS SABDARIFFA L.) TERHADAP BAKTERI STREPTOCOCCUS PNEUMONIAE Komala, Oom; Rosyanti, Reni; Muztabadihardja, Muztabadihardja
BERITA BIOLOGI Vol 12, No 1 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i1.519

Abstract

The in vitro study of antibacterial effectiveness of Rosella (Hibiscus sabdariffa L.) sheath ethanol extract and water extract on Streptococcus pneumoniae was conducted by diffusion and dilution method. Basic solution amounting to 70g/100 ml of the extract was diluted into 10%, 30%, 50% and 70% for diffusion test with positive control ampicillin 10 IU, and 0,1%, 0,25%, 0,5%, 1%, and 2% ethanol extract for dilution test on S. pneumoniae. The results showed that the ethanol extract and water extract could delay of bacterium growth. Agar diffusion test (disk diffusion) was observed by diameter of bar region around the disk. At concentration 70 % among extract ethanol and water extract made bar zone wide the best, the diameter mean was estimated 25.6 mm for ethanol extract, and 24.3 mm for water extract on S. pneumoniae. In dilution test, the result showed Minimum Inhibitory Concentration (MIC) of the extract was 1% on S. pneumoniae. The result of phytochemicals test is known that ethanol extract and water extract of rosella contains of saponin, tanin, and flavonoid.
UJI EFEKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAN EKSTRAK AIR KELOPAK BUNGA ROSELLA (Hibiscus sabdariffa L.) TERHADAP BAKTERI Streptococcus pneumoniae Oom Komala; Reni Rosyanti; Muztabadihardja Muztabadihardja
BERITA BIOLOGI Vol 12, No 1 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i1.519

Abstract

The in vitro study of antibacterial effectiveness of Rosella (Hibiscus sabdariffa L.) sheath ethanol extract and water extract on Streptococcus pneumoniae was conducted by diffusion and dilution method. Basic solution amounting to 70g/100 ml of the extract was diluted into 10%, 30%, 50% and 70% for diffusion test with positive control ampicillin 10 IU, and 0,1%, 0,25%, 0,5%, 1%, and 2% ethanol extract for dilution test on S. pneumoniae. The results showed that the ethanol extract and water extract could delay of bacterium growth. Agar diffusion test (disk diffusion) was observed by diameter of bar region around the disk. At concentration 70 % among extract ethanol and water extract made bar zone wide the best, the diameter mean was estimated 25.6 mm for ethanol extract, and 24.3 mm for water extract on S. pneumoniae. In dilution test, the result showed Minimum Inhibitory Concentration (MIC) of the extract was 1% on S. pneumoniae. The result of phytochemicals test is known that ethanol extract and water extract of rosella contains of saponin, tanin, and flavonoid.
EFEK AROMATERAPI MINYAK ATSIRI MAWAR (Rosa damascena MILL.) DAN KULIT JERUK LIMAU (Citrus amblycarpa) TERHADAP JUMLAH MIKROBA UDARA RUANGAN BERPENDINGIN Oom Komala; Novi Fajar Utami; Siti Mariyam Rosdiana
BERITA BIOLOGI Vol 19, No 2 (2020)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v19i2.3697

Abstract

Energy, material, and or other components were entered to the air by activities of human could cause air quality down to a specific level that could affect human health. The bacteria in the human oral cavity such as Staphylococcus sp. were found in the air during common activities such as coughing, sneezing, and speaking. The research aimed to assess the influence of the combination of oils essential of Rosa damacena and leather oil of Citrus amblycarpa against a decline in the total of microbes on air-conditioned rooms. The method of the research  was used the evaporation of aromatherapy and plate count method. The results were showed that P3 with the concentration of oils essential of R. damacena 2% and leather oil of C. amblycarpa 5% most effective could decrease the total of microbes with an average of 72.5%. Organoleptic, relative density, refractive index, solubility in 90% ethanol, and acid number analysis of Rosa damacena and Citrus amblycarpa leather essential oils met the Indonesian National Standard requirements except for the acid number of leatherlime essential oil and the optical rotation of rose essential oil. It is concluded that the combination of oils essential R. damacena and leather oil of C. amblycarpa  could reduce the total of microbes on air-conditioned rooms.
Co-Authors . Ismanto . Yulianita, . Andhika Dhiasmi Anna Pradiningsih Asmara, Herra Indra Bina Lohita Sari Darda, Rouland Ibnu Dewi Sugiharti Dewi, Elfrieda Kusuma Dhiasmi, Andhika Dina Agustina Durrotun Nafisah Abd.Nf Dwi Indriati E Mulyati Effendi E. Mulyati Effendi Ella Noorlaela Faramitha, Ikhfina Dhea Fatah, Muhammad Fajar Saeful Fitria Dewi Sulistiyono Fuji Raka Siwi Hermawati, Ema Herra Indra Asmara Hery Widijanto Ike Yulia Ike Yulia Wiendarlina Indriati, Dwi Intan Lestari Joshie Ramadhan Lia Puspita Sari Lubis, M. Akhmar Yusfi M. Akhmar Yusfi Lubis Mahyuni, Siti Moerfiah Moerfiah Muhammad Alan Maulana Muztabadihardja Muztabadihardja Muztabadihardja Muztabadihardja Muztabadihardja, Muztabadihardja Muztabadihardja, Muztabadihardja Nina Sakinah Nina Sakinah Noorlaela, Ella Novi Fajar Utami Nurul Fadilah, Nurul Nur’aini, Putri Pebrianti, Rita Prasetyorini Prasetyorini Putri Putri Dwi Antini Putri Nur’aini Rahminiwati, Min Reni Rosyanti Reni Rosyanti Rikkit Rita Pebrianti Rita Rahmawati Rosyanti, Reni Rosyanti, Reni Salsabila, Nadia Muna Saraswati Darmawan Septia Andini Siti Mariyam Rosdiana Sri Suci Damayanti Sri Wardatun Sri Wiedarti Sri Wiedarti Sri Wiedarti Srinarayani, Kadek Sugiharti, Dewi Syamsidar, . Tri Retno Handayani, Marybet Tri Saptari Haryani Triastinurmiatiningsih, . Uswatun Hasanah Vira Mourena Putri Widjaya Yulia, Ike Yulianita Yulianita .