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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Ilmu Dasar Industria: Jurnal Teknologi dan Manajemen Agroindustri Indonesian Journal of Biotechnology and Biodiversity Pena : Jurnal Ilmu Pengetahuan dan Teknologi AKADEMIK: Jurnal Mahasiswa Ekonomi & Bisnis INCOME: Indonesian Journal of Community Service and Engagement Prosiding Seminar Nasional Unimus TARBAWIYAT: Jurnal Kependidikan
Siti Kurniawati
Pusat Penelitian Bioteknologi, Lembaga Ilmu Pengetahuan Indonesia Jl. Raya Bogor Km 46, Cibinong, Bogor 16911, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Control & Systems Engineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Mycobacterium leprae Survival Inside Acanthamoeba sp. Isolated from Water Source in Leprosy Endemic Area, Indonesia: Mycobacterium leprae Survival Inside Acanthamoeba sp. Wahyuni, Ratna; Astari, Linda; Iswahyudi, Iswahyudi; Paling, Sepling; Adriaty, Dinar; Kurniawati, Siti; Aulia, Syifa; Rahmatari, Bandaru; Prakoeswa, Cita Rosita; Agusni, Indropo; Izumi, Shinzo
Journal of Tropical Life Science Vol. 14 No. 2 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.02.07

Abstract

Mycobacterium leprae is an acid-fast bacterium that causes leprosy diseases, which remains a problem worldwide. Even though leprosy prevalence in the world has decreased significantly, many endemic pocket areas continue reporting new cases and harbor M. leprae in the environment, including water and soil. The presence of obligate intracellular bacteria-M. leprae in the environment raises a question on how it survives. Free-living amoeba has been proposed as its reservoir host in the environment. The study was conducted to give evidence that M. leprae can survive inside free-living amoeba isolated from water sources of leprosy endemic areas. M. leprae from leprosy patients was cultured together with Acanthamoeba sp. isolated from the water source of the leprosy endemic area. Viability and duplication of M. leprae inside amoeba then observed at day 14 and 28 using reverse transcriptase PCR and qPCR. The results showed that M. leprae survived inside the amoeba until day 28, but no bacterial replication was observed. The study reveals in vitro evidence of viable M. leprae inside free-living amoeba of leprosy endemic area environment.
Analisis Implementasi Pemanfaatan Lingkungan Sekolah untuk Motivasi Belajar Siswa MI Mata Pelajaran IPA Materi Lingkungan Rosyidah, Af'idatur; Choiriyah, Ma’rifatul; Kurniawati, Siti; Suttrisno, Suttrisno
TARBAWIYAT Vol 3 No 02 (2024): Tarbawiyat: Jurnal Kependidikan
Publisher : Prodi PGMI STAI Al-Akbar Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62589/t.v3i02.375

Abstract

Student motivation to learn is often the main challenge in the learning process, seen from students' lack of attention to the material presented, low active participation, and reluctance to complete assignments. Effective education requires a creative, active, and contextual approach to creating meaningful learning experiences. One potential approach is to utilize the school environment as a learning resource. The school environment, such as parks, gardens, play facilities, and other elements, can be a real and contextual learning medium, allowing students to connect theory with practice. This research aims to analyze the implementation of the use of the school environment in increasing MI students' learning motivation in science subjects, especially environmental material. This research uses a qualitative method with a phenomenological approach, where data is collected through literature studies from various sources such as books, journals and scientific articles. The research results show that the school environment can be used to improve students' cognitive, affective and psychomotor skills, while making learning more enjoyable and effective. Environment-based learning also helps students understand science material, such as healthy and unhealthy environments, more easily. However, there are challenges in its implementation, such as limited school facilities and lack of teacher creativity. This research recommends strategies to maximize the positive impact of using the school environment in supporting student learning motivation holistically.
Deteksi Mycobacteria pada Area Peternakan Unggas di Kabupaten Malang, Jawa Timur: Upaya Pendekatan One Health Kurniawati, Siti; Ramadhani , Najma; Ayu Savitry , Diaz; Titah Pinasti , Roro; Jannah , Miftahul; Ivana Alexsandra Tasumo, Jessica; Gibraltar Makta , Averoes
Pena: Jurnal Ilmu Pengetahuan dan Teknologi Vol. 39 No. 1 (2025): PENA MARET 2025
Publisher : LPPM Universitas Pekalongan

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Abstract

Mycobacteria represent a critical microbial agent with significant implications for animal and human health. Mycobacteria can be found in poultry farm environments, serving as a risk factor for transmission of infection. This study aims to determine the prevalence of Mycobacteria in poultry farming areas. Methods: A total of 25 environmental samples were systematically processed and analyzed using established diagnostic protocols. Mycobacteria identification employed standard microbiological cultivation techniques and acid-fast staining methods. Results The findings revealed an 8% prevalence of Mycobacteria among the examined samples. This prevalence rate demonstrates the bacterial presence within the poultry farming environment, indicating potential transmission risks for both animal and human populations. Conclusion The study successfully documented Mycobacteria presence in poultry farming environments, emphasizing the necessity for continued microbiological surveillance and comprehensive health risk assessments. Further research is recommended to elucidate the specific mechanisms of bacterial transmission and potential mitigation strategies. Keywords: Mycobacteria, Poultry Farm, Soil, Environmental Keywords : consists of, 3-5 terms, in English
Penggunaan Media Pembelajaran Spinning Wheel Pada Mata Pelajaran Bahasa Inggris Di SD Negeri Cibogo Prasetyo, Teguh; Brawijaya , Andri; Fitriliani, Aulya; Kurniawati, Siti
INCOME: Indonesian Journal of Community Service and Engagement Vol 1 No 2 (2022)
Publisher : EDUPEDIA Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (495.228 KB) | DOI: 10.56855/income.v1i2.28

Abstract

This English lesson is taught because it is considered that early childhood is easy to catch new things. The digital age demands, all in English, also encourage children to learn foreign languages and supplement local content at the elementary school level. However, at Elementary School Cibogo, Tugu Jaya Village, Cigombong District, English lessons are still not going well due to the lack of educators (teachers) who master English, so students are still very unfamiliar with English. English tutoring and making Spinning Wheel learning media for English subjects are one of the efforts to assist teachers in conveying English material efficiently to students and introducing English to students. The method of implementing the service consists of the stages of preparation, implementation, and evaluation/reflection of activities. Supporting techniques for the preparation of community service reports are written using techniques, namely observation, and interviews. After the application of the Spinning Wheel media in grade 4 resulted in students becoming enthusiastic when the English learning process took place, it was seen that the students often asked questions about various vocabularies that they did not know. In conclusion, students will be enthusiastic if there is a learning media because it will attract their attention, and this Spinning Wheel is also enough to help teachers. However, teachers also need to deepen the English material).
Komunikasi, Informasi Dan Edukasi Infeksi Penyakit Mulut Dan Kuku Pada Peternak Di KUD Kertajaya Kabupaten Kediri Putri, Salsabila Candra; Kurniawati, Siti; Adrenalin, Sruti Listra
Prosiding Seminar Nasional Unimus Vol 7 (2024): Transformasi Teknologi Menuju Indonesia Sehat dan Pencapaian Sustainable Development G
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Penyakit Mulut dan Kuku (PMK) merupakan penyakit yang disebabkan oleh virus Foot and Mouth DiseaseVirus (FMDV) genus Aphtovirus dan famili Picornaviridae. Penyakit Mulut dan Kuku merupakan penyakitmenular dan akut yang menyerang hewan ternak. Hewan ternak yang dapat terserang penyakit PMK adalahhewan berkuku genap atau berkuku belah seperti sapi, kambing, domba, kerbau, dan babi. Penyakit PMKmemiliki tingkat morbiditas mencapai 100% dan tingkat mortalitas sekitar 1.53% dari total kasus yang ada.Kegiatan KIE dilaksanakan di Desa Medowo, yang dikenal sebagai penghasil susu utama dengan sebagianbesar penduduknya bekerja sebagai peternak sapi perah. Kegiatan KIE bertujuan untuk meningkatkanpengetahuan dan pemahaman peternak terkait pencegahan dan penanganan infeksi PMK. Metode KIE yangdigunakan adalah menggunakan media informasi(pamflet), presentasi, diskusi secara langsung danpenilaian terukur dari hasil pretest, posttest, dan SKM yang dianalisis secara deskriptif dan kuantitatif. Hasilanalisis pretest dan posttest menunjukkan peningkatan yang signifikan, pretest menunjukkan terdapat 59%peternak yang memiliki nilai di atas standar. Setelah pelaksanaan kegiatan KIE, hasil posttest menunjukkanadanya peningkatan sebesar 41% dari hasil sebelumnya. Hasil pengisian SKM juga mendukung kegiatanpenyuluhan, peserta mengakui bahwa kegiatan tersebut relevan dan bermanfaat untuk memperluaspengetahuan para peternak. Kegiatan KIE mengenai PMK yang dilakukan kepada peternak di KUDKertajaya terbukti efektif dalam meningkatkan pemahaman peternak terhadap penyakit PMK. Kata Kunci : PMK, Komunikasi Informasi dan Edukasi, Peternak, KUD Kertajaya.
Komunikasi, Informasi, dan Edukasi Infeksi Bovine Tuberculosis pada Peternak di KUD Kertajaya, Desa Medowo, Kabupaten Kediri Kurniawati, Siti; Putri, Salsabila Candra
Prosiding Seminar Nasional Unimus Vol 7 (2024): Transformasi Teknologi Menuju Indonesia Sehat dan Pencapaian Sustainable Development G
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Bovine tuberculosis (bTB) adalah penyakit infeksi kronis yang disebabkan oleh golongan Mycobacterium tuberculosis complex, yaitu Mycobacterium bovis (M. bovis). M. bovis dapat menginfeksi hewan dan manusia sehingga menjadi ancaman bagi kesehatan hewan dan manusia (zoonosis). Infeksi dapat terjadi melalui kontak langsung dan tidak langsung. bTB menyebabkan lesi pada paru-paru, nodus limfa, dan organ lain yang terinfeksi, yang disertai dengan gejala seperti penurunan berat badan, demam, dan batuk kronis. Dampak dari infeksi ini yaitu adanya penurunan sektor ekonomi signifikan dalam industri peternakan karena penurunan produktivitas, peningkatan mortalitas, serta pengeluaran untuk pengendalian danpengobatan. Penelitian ini bertujuan untuk mengetahui seberapa tingkat pemahaman peternak tentang bTB di KUD Kertajaya, Desa Medowo, Kecamatan Kandangan, Jawa Timur melalui kegiatan Komunikasi, Informasi, dan Edukasi (KIE). KIE ini yaitu diberikan dengan penyuluhan dalam bentuk seminar, diskusiserta pre-test dan post-test. Hasil yang diperoleh terjadi peningkatan nilai 10,42% dari pre-test dan posttest. Kegiatan ini dapat disimpulkan peningkatan pasca kegiatan KIE pada peternak di KUD Kertajaya, Desa Medowo, Kediri, Jawa Timur.Kata Kunci: Bovine tuberculosis, Ternak, M. bovis, zoonosis
Komunikasi, Informasi, dan Edukasi (KIE) dan Penyuluhan Penerapan Sanitasi Sebagai Upaya Menekan Penyebaran Penyakit Mulut dan Kuku (PMK) di KUD Kertajaya, Kabupaten Kediri Subroto, Luthfiyyah Naila Putri; Kurniawati, Siti; Adrenalin, Sruti Listra
Prosiding Seminar Nasional Unimus Vol 7 (2024): Transformasi Teknologi Menuju Indonesia Sehat dan Pencapaian Sustainable Development G
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Penyakit Mulut dan Kuku (PMK) adalah penyakit menular yang berdampak signifikan pada hewan ternak,terutama sapi perah, dan dapat menyebabkan kerugian ekonomi yang besar. Salah satu langkah preventifyang penting dalam menekan penyebaran PMK adalah penerapan sanitasi kandang yang baik. KegiatanKIE ini bertujuan untuk meningkatkan pemahaman peternak mengenai pentingnya sanitasi kandang sebagaiupaya pencegahan PMK. Metode yang digunakan meliputi pretest dan posttest untuk mengukurpengetahuan peternak sebelum dan sesudah penyuluhan. Hasil menunjukkan rata-rata skor pretest 87,5,yang meningkat menjadi 97,5 pada posttest, mencerminkan peningkatan pemahaman sebesar 11,43%.Penerapan sanitasi kandang efektif dalam mencegah penyebaran PMK, dan kegiatan penyuluhan berhasilmeningkatkan pemahaman peternak tentang pentingnya langkah tersebut. Kata Kunci : Penyakit Mulut dan Kuku, Desa Medowo, Peternak
Pola Akumulasi Prolin dan Poliamin Beberapa Aksesi Tanaman Terung pada Cekaman Kekeringan Kurniawati, Siti; Khumaida, Nurul; Ardie, Sintho Wahyuning; Hartati, N. Sri i; Sudarmonowati, Enny
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 42 No. 2 (2014): Jurnal agronomi indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (426.049 KB) | DOI: 10.24831/jai.v42i2.8432

Abstract

ABSTRACT Drought stress is one of the major limiting factors for plant growth and production. Therefore, the mechanism of drought tolerance is important to be studied. Eggplant (Solanum spp.) is relatively tolerant to drought stress compared to other member of Solanaceae family. The objective of this study was to study the mechanism of drought tolerance in eggplant related to accumulation pattern of proline and polyamines. Seven eggplant accessions were drought stressed for 21 days and rewatered for the following next 7 days. Drought stress treatment significantly decreased the soil water content and reduced plant height of all accessions. Eggplant responded the drought stress by reducing stomatal density and stomatal opening to reduce transpiration rate. Drought stress also induced proline and polyamine (PA) accumulation in the leaf tissues. These accumulations started at 14-days after drought stress period when soil water content less than 20% and reached their peaks at 21 days after drought stress. The highest level of proline (134.70 μmol g-1) and that of putrescine (20.836 ng g-1)  could  be used as indicators for eggplant drought stress condition. Keywords: physiological responses, polyamine (PA), proline, putrescine, stomata
Co-Authors Adrenalin, Sruti Listra Amri, Indah Amalia Aulia, Syifa Auranzha, Avriell Jasmine Ayu Savitry , Diaz Brawijaya , Andri Choiriyah, Ma’rifatul Dameanti, Fidi Nur Aini Eka Puji DINAR ADRIATY Enny Sudarmonowati Fitriliani, Aulya Gibraltar Makta , Averoes Hartati Hartati Heldyana, Riskya INDROPO AGUSNI Irwan Raharja ISWAHYUDI ISWAHYUDI Ivana Alexsandra Tasumo, Jessica Jannah , Miftahul Linda Astari, Linda Masardhi, Bagus Aji N. Sri Hartati, N. Sri N. Sri i Hartati Nurul Khumaida Prakoeswa, Cita Rosita Putri, Salsabila Candra Rahmatari, Bandaru Ramadhani , Najma RATNA WAHYUNI Rosyidah, Af'idatur Rosyidah, Af’idatur Sepling Paling Seveline, Seveline SHINZO IZUMI Sintho Wahyuning Ardie Sri Hartati Subroto, Luthfiyyah Naila Putri Suttrisno Teguh Prasetyo Titah Pinasti , Roro