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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Fitopatologi Indonesia Jurnal Ilmu Pertanian Jurnal Natur Indonesia Jurnal Ilmu Pertanian KULTIVAR AGROEKOTEKNOLOGI Kultivasi jurnal Ilmu Pertanian (Agricultural Science) PERTANIAN TROPIK Makara Journal of Science

Luthfi Aziz Mahmud Siregar

Universitas Sumatera Utara

Author-ID : 310019

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Economics, Econometrics & Finance Medicine & Pharmacology

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EVALUASI PERTUMBUHAN DAN PRODUKSI BEBERAPA VARIETAS PADI GOGO (Oryza sativa L.) PADA BEBERAPA JARAK TANAM YANG BERBEDA Fristy Rebecca Hasianta Sitohang; Luthfi Aziz Mahmud Siregar; Lollie Agustina P. Putri
Jurnal Agroekoteknologi Vol 2, No 2 (2014)
Publisher : Universitas Sumatera Utara

Rice is an important commodity in Indonesia. As time went on the narrowing of fertile irrigatedland due to conversion of non-agricultural land for the benefit of making an effort to do that is theuse of improved varieties of upland rice using proper spacing. This study aims to determine themost appropriate spacing and varieties that will enhance the growth and production of uplandrice. The research was carried out on community lahan masyarakat 1 Tanjung Sari, District MedanMedan Selayang Municipal ± 25 m altitude above sea level in the month of June to October2012. This study uses a randomized block design (RBD) with treatment consisting of five varietiesof varieties namely, varieties Situ Bagendit, Situ Patenggang, Towuti, Batutegi and Cirata, andtreatment spacing of three that is 20x20 cm, 20x25 cm and 20x30 cm and the treatment wasrepeated three times.The data obtained were analyzed using analysis of variance, followed byDuncan's Multiple Range Test (DMRT). Results of data analysis showed that the different varietiesof real observations on variables plant height, number of tillers, number of panicles, number ofempty grains, the amount of productive grain, grain weight per sample, 1000 grain weight, and theweight per sample. Spacing is not significantly different on all variables observations. Interactionsbetween plant spacing and different varieties of real variables only on observations of productionper plot.Key words: varieties, spacing, upland rice.

PERTUMBUHAN DAN PERKEMBANGAN NODUS KENTANG (Solanum tuberosum L.) AKIBAT MODIFIKASI KONSENTRASI SUKROSA DAN PENAMBAHAN 2-ISOPENTENILADENINA SECARA IN VITRO Asma Ul Husna; Luthfi Aziz Mahmud Siregar; Yusuf Husni
Jurnal Agroekoteknologi Vol 2, No 3 (2014)
Publisher : Universitas Sumatera Utara

Potato is horticulture crops that able to consume its corm and used as food materials until a raw material toindustry in Indonesia . However, potato production capacity is not proportional to the increase in demand forpotatoes this time, one of this problem due to lack of availability of quality seed. In vitro tecniques is oneway that can be taken by using nodes as seed. Sucrose and cytokinin have an important part on it wheresucrose acts as a carbon source and energy while cytokinin is important in cell division to support a nodesgrowth. The research aimed to get a suitable concentration of sucrose and 2-isopenteniladenine (2-ip) forgrowth of number of potato (Solanum tuberosum L.) nodes by using in vitro methode. The research wascarried out in the Tissue Culture Laboratory, agriculture’s Faculty of North Sumatera University from Juneto September 2013. This research used Randomized Block design with two factors. First factor was sucroseconcentration consist of four level: 35 g/l; 50 g/l; 65 g/l; 80 g/l. The second factor was 2-ip concentrationconsist of five level are 0 mg/l; 2 mg/l; 4 mg/l; 6 mg/l; 8 mg/l. The results showed that sucrose gavesignifiantly effect to number of nodes after darkness period parameter. 2-ip concentration give significantlyeffect only on number of nodes after lighting period. Interaction of sucrose and 2-ip have no effectsignificantly to all parameterKeywords : Sucrose, 2-Isopenteniladenine (2-ip), Potato Nodes, In Vitro

PERTUMBUHAN DAN PERKEMBANGAN TUNAS PUAR TENANGAU (Elettariopsis sp.) AKIBAT PERBEDAAN PERIODE SUB KULTUR Putri Hasanah Jumroh; Luthfi Aziz Mahmud Siregar; Syafruddin Ilyas
Jurnal Agroekoteknologi Vol 2, No 3 (2014)
Publisher : Universitas Sumatera Utara

Puar tenangau is a herbal plant of Zingeberaceae that from Thailand. The Plant has a big potential aspharmacy and traditional medicines, but the spread of this plant was unknown well in Indonesia even soneeded a conservation for this plant. In vitro conservation is a one of alternative. Treatment of periodsubculture is one way to get the explants with optimal growth.The research aimed to determine theappropriate sub culture period to obtain the micropropagation of Puar Tenangau (Elettariopsis sp.) the best asplantlets. The research was carried out in Plant Tissue Culture Laboratory, Department of Agroecotechnology, Faculty ofAgriculture, University of North Sumatera, Medan from November 2012 to August 2013. The research used CompletelyRandomized Design with 1 (one) factor that sub culture period with 4 (four) treatment , which are : 0 week, 2 weeks, 4 weeks,and 6 weeks after culturing from 10 weeks of incubation. The result showed that subculture period not givevaffected on the percentage of explant growt, leaves number, and plant length.Key words : micropopagation, Elettariopsis sp., subculture period

Kajian Awal : Respon Eksplan Nodus dalam Inisiasi Tunas Mikro Tanaman Karet (Hevea brasiliensis Muell Arg.) dalam Medium MS Permata Sari Harahap; Luthfi Aziz M. Siregar; Yusuf Husni
Jurnal Agroekoteknologi Vol 3, No 1 (2015)
Publisher : Universitas Sumatera Utara

The aimed of the research was to determine the best medium for micro shoot induction ofrubber plant (H. brasiliensis Muell. Arg.) from node explant in MS medium. Theresearch was carried out in the In Vitro Culture Laboratory, PT. Perkebunan NusantaraIII Kebun Gunung Pamela, Tebing Tinggi, Sumatera Utara, Indonesia. The researchbegan from April to June 2014. The research used Completely Randomized Design withsixteen treatments and six replications. The results showed that combination of BAP andNAA concentrations significantly affected on percent of sterile explants and percent ofshoots emergence. Each treatment showed different result and the MS Media + 1 mg/lBAP + 0 mg/l NAA was the best media for leaf formation of the microshoot.Key words: rubber, micro shoot, BAP, NAA

Analisis Keragaman Genetik Andaliman (Zanthoxylum acanthopodium DC.)Sumatera Utara Menggunakan Marka RAPD (Random Amplified Polymorphic DNA) Ayu Oshin Yap Sinaga; Lollie Agustina P. Putri; Luthfi Aziz M. Siregar
Jurnal Agroekoteknologi Vol 3, No 1 (2015)
Publisher : Universitas Sumatera Utara

The aim of the research was to analyze the genetic diversity of the endemic andaliman inNorth Sumatera based on RAPD markers. A total of 30 accessions andaliman originated fromthe various region in North Sumatera, i.e.: Dairi, Karo Highland, Simalungun. The RAPDanalyse was used with 3 random primer: OPH-06, OPC-12 and OPN-03. These three primersshowed 32 bands, on which 31 were polymorphic (95.23%). Further genetic diversitycoefficient and the filogenetic dendogram were obtained using the Darwin 5.05 software. Theresults showed that 30 accessions of andaliman were clustered in three groups. Each groupconsisted of the accessions of three regions from different altitudes. There were 30 accessionsof three regions studied showed a high genetic diversity.Keywords: Andaliman, genetic diversity, random amplified polymorphic DNA

Kajian Awal : Respon Eksplan Nodus terhadap Inisiasi Tunas Mikro Tanaman Karet (Hevea brasiliensis Muell. Arg.) pada Media dan Genotipe Berbeda Heri Hidayat; Luthfi Aziz M. Siregar; Eva Sartini Bayu
Jurnal Agroekoteknologi Vol 3, No 4 (2015)
Publisher : Universitas Sumatera Utara

The aim of the research was to determine the proper medium for micro shoot induction of rubber plant from the nodes explants of diffrent genotypes. The research was conducted at the rubber plant Micocutting Laboratory PT. Perkebunan Nusantara III Kebun Gunung Pamela, Tebing Tinggi, North Sumatera, Indonesia. The research began from September to December 2014. the completely randomized design was used with twenty four treathments and five replications. The result showed that the genotype and diffrent composition medium gave significantly diffrent on percent of explant forming shoot. Meanwhile, for parameter life explant the genotype and diffrent composition medium no significantly diffrent.Keywords: rubber, micro shoot, genotype, in vitro medium

Respon GA3 Terhadap Induksi Tunas Mikro Tanaman Karet (Hevea brasiliensis (Muell). Arg) Larosa Harahap; Luthfi Aziz Mahmud Siregar; Diana Sofia Hanafiah
Jurnal Agroekoteknologi Vol 4, No 1 (2016)
Publisher : Universitas Sumatera Utara

The aim of the research was to know effect of GA3 to rubber plant in micro shoot formation. The research was conducted at the rubber plant microcutting labortory PT Perkebunan Nusantara III Kebun Gunung Pamela, Tebing Tinggi, North Sumatera, Indonesia from April 2015 to Juli 2015. The Completely randomize design was used with two factors,i.e.:the addition of GA3 (GA3 0 mg/l; GA3 0.5 mg/l; GA3 1 mg/l; GA3 1.5 mg/l) and the immersion of nodes (GA3 0 mg/l; GA3 5 mg/l; GA3 10 mg/l; GA3 15 mg/l) with seven replications. The result showed that addition of 0.5 mg/l GA3 of significantly affected the percent of shoot emergence but no significantly different age of shoot emergence and immersion of nodes of 0 mg/l significantly affected the percent of shoot emergence but no significantly different age of shoot emergence and the interraction that addition of 0.5 mg/l GA3 and immersion of nodes significant affected the percent of shoot emergence on age of shoot emergence but no significantly different.Keywords : rubber, micro shoot, addition of GA3, immersion of nodesThe aim of the research was to know effect of GA3 to rubber plant in micro shoot formation. The research was conducted at the rubber plant microcutting labortory PT Perkebunan Nusantara III Kebun Gunung Pamela, Tebing Tinggi, North Sumatera, Indonesia from April 2015 to Juli 2015. The Completely randomize design was used with two factors,i.e.:the addition of GA3 (GA3 0 mg/l; GA3 0.5 mg/l; GA3 1 mg/l; GA3 1.5 mg/l) and the immersion of nodes (GA3 0 mg/l; GA3 5 mg/l; GA3 10 mg/l; GA3 15 mg/l) with seven replications. The result showed that addition of 0.5 mg/l GA3 of significantly affected the percent of shoot emergence but no significantly different age of shoot emergence and immersion of nodes of 0 mg/l significantly affected the percent of shoot emergence but no significantly different age of shoot emergence and the interraction that addition of 0.5 mg/l GA3 and immersion of nodes significant affected the percent of shoot emergence on age of shoot emergence but no significantly different.Keywords : rubber, micro shoot, addition of GA3, immersion of nodes

Pengaruh Jenis Eksplan dan Komposisi Media terhadap Pembentukan Tunas Tanaman Karet (Hevea brasiliensis Muell. Arg.) Secara In Vitro Emmy Rosita; Luthfi Aziz Mahmud Siregar; Emmy Harso Kardhinata
Jurnal Agroekoteknologi Vol 4, No 1 (2016)
Publisher : Universitas Sumatera Utara

The aim of this research is to evaluate the influence of medium composition and types of explant on shoot formation of rubber tree (H. brasiliensis Muell. Arg.). The research was carried out in the In Vitro Culture Laboratory, PT. Perkebunan Nusantara III Kebun Gunung Pamela, Tebing Tinggi, Sumatera Utara, Indonesia. The research was conducted from March to July 2015. The research was arranged in completely randomized design with two factors, i.e.: types of explant and the medium with combination of growth regulatorsand fifteen replications. The results showed that interaction types of explant and medium with combination of growth regulators gave significant effect on percent of shoots.Key words: rubber, multiplication, explants, medium in vitroThe aim of this research is to evaluate the influence of medium composition and types of explant on shoot formation of rubber tree (H. brasiliensis Muell. Arg.). The research was carried out in the In Vitro Culture Laboratory, PT. Perkebunan Nusantara III Kebun Gunung Pamela, Tebing Tinggi, Sumatera Utara, Indonesia. The research was conducted from March to July 2015. The research was arranged in completely randomized design with two factors, i.e.: types of explant and the medium with combination of growth regulatorsand fifteen replications. The results showed that interaction types of explant and medium with combination of growth regulators gave significant effect on percent of shoots.Key words: rubber, multiplication, explants, medium in vitro

Karakteristik Pertumbuhan Vegetatif dari Beberapa Aksesi Tanaman Bangun-Bangun (Plectranthus amboinicus (Lour.) Spreng) nuraminah nasution; Luthfi Aziz M. Siregar; Eva Sartini Bayu
Jurnal Agroekoteknologi Vol 5, No 1 (2017)
Publisher : Universitas Sumatera Utara

Penelitian bertujuan untuk mengetahui karakteristik pertumbuhan vegetatif, kandungan sterol dan klorofil dari beberapa aksesi tanaman bangun-bangun. Penelitian dilaksanakan di rumah kasa Fakultas Pertanian, Universitas Sumatera Utara Medan, dimulai Desember 2015 sampai Februari 2016 menggunakan tanaman bangun-bangun asal Medan (Krakatau), Medan (Tuntungan), Sibolangit, Simalungun dan Brastagi. Hasil penelitian menunjukkan bahwa karakter-karakter morfologi pada aksesi tanaman asal Medan (Tuntungan), Sibolangit, Simalungun dan Brastagi tidak berbeda nyata, tetapi berbeda nyata dengan aksesi tanaman asal Medan (Krakatau) pada karakter warna batang dan warna permukaan daun bagian atas.

Pengaruh Jenis Eksplan dan Komposisi Zat Pengatur Tumbuh Terhadap Induksi Kalus Pada Tanaman Binahong (Anredera cordifolia (Ten.) Steenis) Ira Tiarma Sari Damanik; Rosmayati Rosmayati; Luthfi Aziz Mahmud Siregar
Jurnal Agroekoteknologi Vol 5, No 3 (2017)
Publisher : Universitas Sumatera Utara

Penelitian ini bertujuan untuk mengetahui pengaruh jenis eksplan dan komposisi zat pengatur tumbuh terhadap induksi kalus pada tanaman binahong (Anredera cordifolia (Ten.) Steenis). Penelitian ini dilaksanakan di Laboratorium Kultur Jaringan, Dinas Pertanian Sumatera Utara pada bulan Juli sampai dengan September 2016. Rancangan yang digunakan adalah rancangan acak lengkap dengan 2 faktor yaitu jenis eksplan (daun, batang, tangkai daun) dan zat pengatur tumbuh (MS; MS + 1 mg/L 2,4 D + 0,5 mg/L BAP; MS + 2 mg/L 2,4 D + 0,5 mg/L BAP; MS + 3 mg/L 2,4 D + 0,5 mg/L BAP; MS + 1 mg/L 2,4 D + 1 mg/L BAP; MS + 2 mg/L 2,4 D + 1 mg/L BAP; MS + 3 mg/L 2,4 D + 1 mg/L BAP). Hasil penelitian menunjukkan jenis eksplan berpengaruh nyata terhadap persentase terbentuk kalus. Komposisi zat pengatur tumbuh berpengaruh nyata terhadap persentase terbentuk kalus. Eksplan batang merupakan eksplan yang terbaik. Media MS + 3 mg/L 2,4 D + 0,5 mg/L BAP, MS + 2 mg/L 2,4 D + 1 mg/L BAP, dan MS + 3 mg/L 2,4 D + 1 mg/L BAP merupakan media yang terbaik untuk persentase terbentuk kalus.

Co-Authors Abd. Rasyid Syamsuri Abdul Rauf Agus Pernando Simanjuntak Asima napitupulu Asma Ul Husna Ayu Oshin Yap Sinaga Beatrix Blandina BOEY PENG LIM Boey Peng-Lim CHAN LAI KENG Chan Lai-Keng Chandri Lidya Putri Nasution Diana Sofia Hanafiah DWI NINGSIH SUSILOWATI Dyanne Gisella P. Tambak Elseria Siburian Emmy Harso Kardhinata Emmy Rosita Erni Royani Harahap Eva Sartini Bayu Eva Sartini Bayu Firdani, Citra Fristy Rebecca Hasianta Sitohang Hanipah Tanjung Hapsoh Hasmawi Hasyim2 Hasmawi Hasyim2 Heri Hidayat Hilda Mei Yeni Harahap Hot Setiado Hot Setiado Hot Setiado I.M. Revandi Damanik Imam, muhammad Ira Tiarma Sari Damanik Julita Julita Keng, Chan Chan Khairunnisa Lubis Khairunnisa Lubis Lambok Turnip Larosa Harahap Lili Herawati Siregar Linda Tri Wira Astuti Lisa Lisa Mawarni Lisnawita Lita Adelia Lollie A. P. Putri Agustina P Putri Lollie Agustina P Putri Lollie Agustina P. Putri Lollie Agustina P. Putri Lollie Agustina P. Putri Lollie Agustina P. Putri Mahyuddin Dalimunte Marpaung, Agustina Erlinda Milani, Ayu Mona Faronika1 Mona Faronika1 Mutia Rahmah Nazriah pratiwi Nella Angelina Simanjuntak Nina Unzila Angkat nuraminah nasution Permata Sari Harahap Purba, Hans Samuel Putri Hasanah Jumroh Retno Diah Setiowati Revandy Iskandar Muda Damanik Riski Aulia Risma Damayanti Rosmayati Rosmayati Rosmayati Rosmayati Rosmayati Rosmayati Rozaliana Rozaliana Siti Aminah Sopa Putri Tanjung Tengku Sabrina Triningsih Triningsih Ulfa Ridha Andini Utri Patma1 Utri Patma1 Yoppi Operasisco Gabesius Yusuf Husni Yusuf Husni

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