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All Journal Berkala Kedokteran Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Homeostasis: Jurnal Mahasiswa Pendidikan Dokter
Muthmainah, Noor
Universitas Lambung Mangkurat
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health

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Literature Review: Pola Kepekaan Salmonella typhi terhadap Antibiotik pada Pasien Dewasa Rojwa Hafizhah; Noor Muthmainah; Agung Biworo
Homeostasis Vol 4, No 3 (2021)
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Abstract: Typhoid fever is an acute systemic infectious disease caused by a bacterium called Salmonella typhi (S. typhi). The sensitivity of bacteria to antibiotics can change over time, it can be affected by the location and by how often an antibiotic is used. The general aim of this article is to provide an overview of the development of the susceptibility pattern of Salmonella typhi to antibiotics in adult patients in several countries in recent years. Writing is done by analyzing related literature obtained from PubMed – MEDLINE and Google Scholar. From the literature analysis in several countries (India, Pakistan, Bangladesh, Nepal, Nigeria, Indonesia, Ethiopia, Fiji) and Sub-Saharan Africa which were included in this study, antibiotics that are still sensitive are azithromycin, ceftriaxone, chloramphenicol, cotrimoxazole, ciprofloxacin. , ofloxacin, amikacin and ampicillin so that this antibiotic is still recommended in the treatment of typhoid fever. Antibiotics that were found to be resistant were nalidixic acid, tetracycline, amoxicillin and erythromycin. Keywords: pattern of sensitivity, resistance, Salmonella typhi, antibiotics, adult patients Abstrak: Demam tifoid ialah suatu penyakit infeksi sistemik yang bersifat akut, disebabkan oleh suatu bakteri yang bernama Salmonella typhi (S. typhi). Sensitivitas bakteri terhadap antibiotik dapat berubah seiring berjalannya waktu, dapat dipengaruhi oleh tempat serta dari seberapa sering suatu antibiotik digunakan. Tujuan umum dari artikel ini adalah memberikan gambaran umum tentang perkembangan pola kepekaan Salmonella typhi terhadap antibiotik pada pasien dewasa di beberapa negara dalam beberapa tahun terakhir. Penulisan dilakukan dengan menganalisis literatur terkait yang didapatkan dari PubMed–MEDLINE dan Google Scholar. Dari analisis literatur di beberapa negara (India, Pakistan, Bangladesh, Nepal, Nigeria, Indonesia, Ethiopia, Fiji) dan Wilayah Sub-Sahara Afrika yang dimasukkan ke dalam studi ini, antibiotik yang masih sensitif adalah azitromisin, seftriakson, kloramfenikol, kotrimoksazole, siprofloksasin, ofloksasin, amikasin dan ampisilin sehingga antibiotik ini masih direkomendasikan dalam pengobatan demam tifoid. Antibiotik yang didapatkan resisten adalah asam nalidiksat, tetrasiklin, amoksisilin dan eritromisin. Kata-kata kunci: pola kepekaan, resistensi, Salmonella typhi, antibiotik, pasien dewasa
Gambaran Karakteristik dan Penyakit Komorbid pada Pasien Konfirmasi Covid-19 di RSUD Ulin Banjarmasin Tahun 2020 Agnes Angelina Laila Cibro; Nika Sterina Skripsiana; Noor Muthmainah; Farida Heriyani; Nani Zaitun
Homeostasis Vol 5, No 1 (2022)
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Abstract: Coronavirus Disease 2019 (COVID-19) is an irresistible sickness brought about by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). The spread of COVID-19 has turned into a worldwide wellbeing danger until May 13, 2020, there were 4 million affirmed instances of COVID-19 with 287,399 passings announced in excess of 210 impacted nations around the world. This study expects to depict the attributes old enough, orientation, instruction and occupation and comorbid infections in affirmed COVID-19 patients at Ulin Hospital Banjarmasin. This study is a  descriptive study  involving auxiliary information as understanding clinical records at the Ulin Regional General Hospital Banjarmasin period March-December 2020. Data analysis using the method sampling purposive. The number of samples is 219 people. The results showed that the dominant patient characteristics were age 50 years (60.7%), male gender (50.7%), high school education (51.4%) and self-employed (30.1%) . Meanwhile, the predominant comorbid disease was diabetes mellitus (21.6%) Keywords: COVID-19, characteristics, comorbid disease, confirmed patient.  Abstrak: Coronavirus Disease 2019 (COVID-19) adalah penyakit menular yang disebabkan oleh Sindrom Pernafasan Akut Parah Coronavirus 2 (SARS-CoV-2). Penyebaran COVID-19 telah menjadi ancaman kesehatan di seluruh dunia hingga 13 Mei 2020, ada 4 juta kasus dikonfirmasi COVID-19 dengan 287.399  kasus kematian diumumkan di lebih dari 210 negara yang terkena dampak di seluruh dunia. Penelitian ini diharapkan dapat menggambarkan karakteristik berdasarkan umur, jenis kelamin, pendidikan dan pekerjaan serta penyakit komorbid  pada pasien konfirmasi COVID-19 di RSUD Ulin Banjarmasin. Penelitian ini merupakan penelitian deskriptif dengan data sekumder rekam medis pada pasien konfirmasi COVID-19  di RSUD Ulin Banjarmasin periode Maret-Desember 2020. Analisis data menggunakan metode sampling purposive. Jumlah sampel sebanyak 219 orang. Hasil penelitian menunjukkan bahwa karakteristik pasien yang dominan adalah usia 50 tahun (60,7%), jenis kelamin laki-laki (50,7%), pendidikan SLTA (51,4%) dan wiraswasta (30,1%). Sedangkan penyakit penyerta yang dominan adalah diabetes mellitus (21,6%) Kata-kata kunci: COVID-19, karakteristik, penyakit komorbid, pasien konfirmasi
Perbandingan Aktivitas Antibakteri Ekstrak Daun Tanjung dan Daun Jambu Biji terhadap Staphylococcus aureus In Vitro Irhamna Syari Yani Yani; Noor Muthmainah; Alfi Yasmina
Homeostasis Vol 3, No 2 (2020)
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Abstract: Tanjung plants and guava plants are known as plants by the community as herbal medicines. Tanjung leaves contain anti-bacterial substances such as flavonoids, tannins, terpenoids and saponins, while guava leaves are flavonoids and alkaloids. This study aims to determine comparison the antibacterial activity of tanjung and guava leaves S. aureus in vitro. This research uses an experimental method, grouped each cape leaf and guava leaf. One group based on concentrations of 5%, 15%, 25%, and 35% obtained inhibitory zones in cape leaf extract in a row of 8.9 mm, 12.32 mm, 15.49 mm and 17.31 mm, in jamb bji leaf extract were 7.08 mm, 11.09 mm, 11.34 mm and 16.58 mm respectively. Positive control in this study used clindamycin and negative control was aquades. Data analysis was performed with Shapiro-Wilk normality test, Levene’s Test variance homogeneity test obtained normal and non-homogeneous data then continued with the Kruskall-Wallis and Mann Whitney tests with a 95% confidence level. In this study, there was no significant difference between tanjung leaf extract and guava leaf extract. Conclusions, there were no significant differences in the activity of tanjung leaf extract and guava leaf extract to inhibit the growth of S. aureus in vitro. Keywords:        Tanjung leaves, guava leaves, Staphylococcus aureus Abstrak:  Tanaman tanjung dan tanaman jambu biji merupakan tanaman yang digunakan oleh masyarakat sebagai obat herbal. Daun tanjung mengandung zat anti bakteri seperti flavonoid, tanin, terpenoid dan saponin, sedangkan pada daun jambu biji terdapat flavonoid dan alkaloid. Penelitian ini bertujuan untuk mengetahui perbandingan aktivitas antibakteri daun tanjung dan daun jambu biji terhadap Staphylococcus aureus secara in vitro. Penilitian ini menggunakan metode eksperimental, dikelompokkan masing-masing, satu kelompok berdasarkan konsentrasi   5%, 15%, 25%, dan 35% didapatkan zona hambat pada ekstrak daun tanjung secara berturut-turut sebesar 8,9 mm, 12,32 mm, 15,49 mm dan 17,31 mm, ekstrak daun jambu biji berturut-turut 7,08 mm, 11,09 mm, 11,34 mm dan 16,58 mm. Kontrol psitif pada penelitian ini menggunakan klindamisin dan kontrol negatif adalah aquades. Melakukan analisis data dengan uji normalitas  Shapiro-Wilk, uji homogenitas varians Levene’s Test didapatkan data normal dan tidak homogen maka dilanjutkan dengan uji Kruskall-Wallis dan Mann Whitney dengan tingkat kepercyaan 95%. Pada penelitian ini tidak ditemukan berbeda bermakna antara ekstrak daun tanjung dengan ekstrak daun jambu biji.”Kesimpulan padaa peneliitian ini  tidak terdapaat perbedaan bermakna pada aktivitas ekstrak daun tanjung dan ekstrak daun jambu biji untuk menghambat pertumbuhan S. aureus secara in vitro. Kata – kata kunci :  daun tanjung, daun jambu biji, Staphylococcuss aureus,
Identifikasi Bakteri pada Luka Operasi Pasien Paska Seksio Sesarea di Bangsal Kandungan dan Kebidanan RSUD Ulin Banjarmasin Risa Dirgagita; Renny Aditya; Noor Muthmainah
Homeostasis Vol 3, No 3 (2020)
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Abstract: To to find out the description of the type of bacteria in post-cesarean surgical patient wounds obtained through the patient's surgical wound swab in the Ward Obstetrics and Gynecology RSUD Ulin Banjarmasin in the period August-October 2019. This study was ‘a descriptivee study with a cross sectional’ appproach. A total of 36 samples were taken using purposive sampling method, but only 32 bacterial isolates were obtained. Samples in the form of clean operating wound contaminated post-cesarean patients were planted on growth media and identified microscopically to be further classified based on responses to biochemical tests. Descriptive analysis shows that there are three types of bacteria, namely Staphylococcus aureus as much as 59.3%, Staphylococcus epidermidis as much as 25.0%, and Escherichia coli as much as 15.6%. Based on research conducted, obtained 3 types of bacteria in the results of surgical wound swab in post cesarean section patients who were hospitalized in the Ward and Obstetrics Hospital of Ulin Hospital Banjarmasin, namely Staphylococcus aureus as much as 59.4%, Staphylococcus epidermidis as much as 25.0%, Escherichia coli as much as 15.6%. Keywords: SSI, caesarean section, contaminant bacteria, surgery wound swab Abstrak: Untuk mengetahui gambaran jenis bakteri pada luka operasi pasien paska seksio sesarea yang didapat melalui ‘swab pada luka operasi pasien yang ada di Bangsal Kandungan dan Kebidanan RSUD Ulin Banjarmasin dalam periode Agustus-Oktober 2019. Penelitian ini adalah studi dekskriptif dengan pendekatan cross sectional. Sebanyak 36 hasil sampel diambil ‘dengan metode purposive sampling, tetapi hanya didapatkan 32 isolat bakteri. Sampel swab luka operasi bersih terkontaminasi pasien paska seksio sesarea ditanam pada media pertumbuhan dan diidentifikasi secara mikroskopis untuk selanjutnya diklasifikasikan berdasarkan respon terhadap uji biokimia. Analisis secara deksriptif menunjukkan bahwa terdapat tiga jenis bakteri, yaitu Staphylococcus aureus’ sebesar presentasinya 59,3%, Staphylococcus epidermidis’ sebesar 25,0%, dan Escherichia coli sebesar’ 15,6%. Berdasarkan penelitian yang dilakukan, didapatkan 3 jenis bakteri pada hasil swab luka operasi pasien paska seksio sesarea yang dirawat inap di Bangsal Kandungan dan Kebidanan RSUD Ulin Banjarmasin, adalah Staphylococcus aureus sebanyak 59,4%, Staphylococcus epidermidis sebanyak 25,0%, Escherichia coli sebanyak 15,6%. Kata-kata kunci: IDO, seksio sesarea, bakteri kontaminan, swab luka operasi
Hubungan antara Pengetahuan dengan Sikap dan Perilaku Pencegahan Covid-19 pada Siswa SMA Negeri 3 Banjarmasin Roselya Elvaniah; Noor Muthmainah; Nika Sterina Skripsiana; Farida Heriyani; Nani Zaitun
Homeostasis Vol 5, No 2 (2022)
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Abstract: At this time, COVID-19 is a global case that really needs attention, because there are always spikes in cases every day. This study aims to determine the relationship between knowledge, attitudes and behavior of SMA Negeri 3 Banjarmasin students in an effort to prevent COVID-19. The research method is analytic observational, with a cross sectional approach. The sample is 86 students of SMA Negeri 3 Banjarmasin using a sample technique that is proportionate stratified random sampling. Data analysis in this study used the chi-square test. The results of the univariate analysis showed that as many as 60 people (69.8%) had knowledge, 68 people (79.1%) had a good attitude, and 64 people (74.4%) had good behavior in preventing COVID-19. 19 prevention Bivariate analysis of the relationship with attitude, yielded a p-value of 0.000 (p<0.05), obtained a relationship between the level of knowledge and behavior related to COVID-19 prevention with a p-value of 0.019 (p<0.05). Keywords: knowledge, attitude, behavior, COVID-19, high school students Abstrak: Pada saat ini, COVID-19 adalah kasus dunia yang sangat perlu diperhatikan, karena senantiasa terjadi lonjakan kasus setiap harinya. Penelitian ini bertujuan untuk mengetahui hubungan antara pengetahuan, sikap dan perilaku siswa SMA Negeri 3 Banjarmasin dalam upaya pencegahan COVID-19 Metode riset yaitu observasional analitik, dengan  pendekatan cross sectional. Sampel sebanyak 86 siswa SMA Negeri 3 Banjarmasin menggunakan teknik sampel yaitu proportionate stratified random sampling. Analisis data dalam penelitian ini menggunakan uji chi-square. Hasil analisis univariat menunjukkan bahwa sebanyak 60 orang (69,8%) berpengetahuan, 68 orang (79,1%) memiliki sikap baik, dan 64 orang (74,4%) memiliki perilaku baik dalam pencegahan COVID-19. 19 pencegahan Analisis bivariat hubungan dengan sikap, menghasilkan p-value 0,000 (p<0,05), diperoleh hubungan antara tingkat pengetahuan dan perilaku terkait pencegahan COVID-19 dengan hasil p-value 0,019 (p<0,05). Kata-kata kunci: pengetahuan, sikap, perilaku, COVID-19, siswa SMA
Gambaran Most Probable Number Air Galon Bermerek dan Isi Ulang di Banjarmasin Rosmitha Monikayani; Husnul Khatimah; Noor Muthmainah; Rahmiati Rahmiati; Ika Kustiyah Oktaviyanti
Homeostasis Vol 3, No 1 (2020)
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Abstract: Branded gallon water and refill gallon water become the primary choice of Indonesian people to fill their demand to daily drinking water. PERMENKES number 492 in 2010 stated that water for consumption shouldn’t contain any coliform bacteria. The purpose of this study was to describe Most Probable Number in branded gallons water and refill gallons water in Banjarmasin. The method used in this study was descriptive cross-sectional. This MPN test was using table of Thomas 511. The results showed that from 15 of 15 samples branded gallons water in Banjarmasin, the Most Probable Number were 0/100mL. Besides, refill gallons water in Banjarmasin from 5 of 15 samples showed that the Most Probable Number were 2/100mL. It means branded gallons water in Banjarmasin didn’t contain any coliform bacteria while refill gallons water in Banjarmasin have to give control to depots because it was contain coliform bacteria. Keywords: most probable number (mpn), branded gallons water, refill gallons water. Abstrak: Air galon bermerek dan isi ulang menjadi pilihan utama masyarakat Indonesia untuk memenuhi kebutuhan air minum sehari-hari. PERMENKES No.492 tahun 2010 mengatur bahwa air yang dikonsumsi tidak boleh mengandung bakteri coliform. Tujuan penelitian ini adalah untuk mengetahui gambaran Most Probable Number air galon bermerek dan isi ulang di Banjarmasin. Metode yang digunakan dalam penelitian ini adalah deskriptif dengan pendekatan cross-sectional. Uji Most Probable Number ini menggunakan tabel MPN Thomas 5 1 1. Hasil penelitian 15 dari 15 sampel air galon bermerek di Banjarmasin mempunyai nilai Most Probable Number 0/100mL. Sedangkan, air galon isi ulang di Banjarmasin menunjukan 5 dari 15 sampel mendapatkan nilai Most Probable Number 2/100mL. Hal ini menunjukan bahwa air galon bermerek di Banjarmasin tidak mengandung bakteri coliform, sedangkan air galon isi ulang di Banjarmasin perlu dilakukan pengawasan yang lebih karena mengandung bakteri coliform. Kata-kata kunci: most probable number (mpn), air galon bermerek, air galon isi ulang.
Perbandingan Aktivitas Antibakteri Ekstrak Daun Tanjung dan Daun Jambu Biji Terhadap Salmonella typhi In Vitro Dini Rizki Aulia; Noor Muthmainah; Alfi Yasmina
Homeostasis Vol 3, No 1 (2020)
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Abstract: Tanjung and guava plant have been known as the famous plant in Indonesia used for antidiarrhea. The active ingredientts of ethanol’ extract of tanjung leaves are alkaloid, tanin and saponin, while the active ingredients of ethanol extract of guava leaves are alkaloid and flavonoid. The purpose of this study was to assess the comparison of antibacterial activity between tanjung leaves and guava leaves ethanol extracts against Salmonella typhi in vitro. The design of the study was true experimental with Posttest-Only with Control Group Design method, using random design group which consisted of the following concentrations of both extract; 6.25%, 12.5%, 25%, 50%, and with chloramphenicol 30 µg as positiv control and aquades as negative conttrol.  The statistical analysis in this study used Shapiro-Wilk, Levene’s Test, Kruskal-Wallis and Mann-Whitney tests. The average of largest inhibitory zone of both extracts with the concentration of 50% was 15.74 mm for tanjung leaves ethanol extract and 14.99 mm for guava leaves ethanol extract. There was significant differences of inhibitory zone diameters between tanjung leaves and guava leaves extract at the concentration of 6.25%, 12.5%, and 50% (p < 0,05). The conclusion of this study showed that ‘the tanjung leaves ethanol extract has significantly higher average of inhibitory zone compared to guava leaves ethanol extract against S. typhi. Keywords: tanjung leaves, guava leaves, Salmonella typhi. Abstrak: Tanaman tanjung dan jambu biji adalah tanaman yang sudah terkenal lama di Indonesia sebagaii antidiare. Kandungan zat aktif yang terdapat pada ekstrak etanol daun tanjung meliputi senyawa alkaloid, tanin, dan saponin,sedangkan ekstrak etanol daun jambu biji memiliki kandungan zat aktif berupa senyawa alkaloid dan flavonoid. Tujuan dari penelitian ini untuk mengetahui perbandingan aktiivitas antibakteri esktrak etanol daun tanjung dan daun jambu biji ‘terhadap Salmonella typhi in vitro. Penelitian ini merupakan penelitian true experimental dengan metode Posttest-Only with Control Group Design, menggunakan rancangan penelitian acak kelompok yang terdiri dari perlakuan konsentrasi ekstrak etanol daun tanjung dan daun jambu biji sebesar 6,25%, 12,5%, 25%, 50% serta kloramfenikol 30 µg sebagai kontrol positif dan aquadest sebagai kontrol negatif. Analisis data penelitian menggunakan uji Shapiro-Wilk, Levene’s Test, Kruskal-Wallis dan Mann-Whitney, dengan tingkat kepercayaan 95%. Rerata zona hambat terbesar yang dihasilkan ekstrak daun tanjung dan daun jambu biji dengan konsentrasi 50% terhadap pertumbuhan S. typhi secara berturut-turut sebesar 15,74 mm dan 14,99 mm. Terdapat perbedaan bermakna diameter zona hambat antara ekstrak daun tanjung dan jambu biji pada konsentrasi 6,25%, 12,5%, dan 50% (p < 0,05). Kesimpulannya adalah ekstrak etanol daun tanjung memiliki rerata zona hambat yang lebih besar secara bermakna dibandingkan ekstrak etanole daun jambu biji terhadap pzertumbuhan S. typhi Kata-kata kunci: daun tanjung, daun jambu biji, Salmonella typhi
Identifikasi Bakteri Kontaminan Udara di Ruang Perinatologi RSUD Idaman Banjarbaru Tahun 2018 Larasati Gilang Puji Astuti; Noor Muthmainah; Rahmiati Rahmiati
Homeostasis Vol 2, No 1 (2019)
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Abstract: Nosocomial infection can be caused due to the quality mikrobiological of air in the Perinatology room. Cause infection can be transmitted through the air. The aim this study of determine types of airborne contaminant bacteria in the Perinatology room RSD Idaman Banjarbaru period July 2018. This study uses descriptive method and cross-sectional approach. Identification of bacteria were performed using 15 blood agar media and 15 Mac Conkey media. Sampling was carried out using open plate method. The results of the study obtained gram-positive bacteria Staphylococcus aureus 48,6%, Staphylococcus epidermidis 12,5 %, and Staphylococcus saprophyticus 5,0 %. Gram negatif bacteria Escherichia coli 27,2 % and Salmonella typhi 6,7 %. The conclusions of the study gram-positive bacteria more found than gram-negatif.  Keywords: airborne contaminant bacteria, Perinatology room, open plate Abstrak: Infeksi nosokomial dapat disebabkan karena kualitas mikrobiologi udara di ruang Perinatologi, karena transmisi mikroorganisme penyebab infeksi dapat ditularkan melalui udara. Tujuan penelitian ini adalah untuk mengetahui jenis-jenis bakteri kontaminan udara di ruang Perinatologi RSD Idaman Banjarbaru periode Juli 2018. Metode penelitian adalah deskriptif dan pendekatan cross sectional. Identifikasi bakteri dilakukan dengan menggunakan 15 media agar darah dan 15 media Mac Conkey. Pengambilan sampel dengan metode open plate. Hasil penelitian didapatkan bakteri gram positif yaitu Staphylococcus aureus 48,6 %, Staphylococcus epidermidis 12,5 % dan Staphylococcus saprophyticus 5,0 %. Bakteri gram negatif yaitu Escherichia coli 27,2 % dan Salmonella typhi 6,7 %. Kesimpulan dari penelitian ini yaitu bakteri gram positif lebih banyak ditemukan dibandingkan bakteri gram negatif. Kata-kata kunci: bakteri kontaminan udara, ruang Perinatologi, open plate
Identifikasi Jamur Kontaminan Udara di Ruang Intensive Care Unit (ICU) RSUD Idaman Banjarbaru Tahun 2018 Muhammad Fajariyanoor; Noor Muthmainah; Rahmiati Rahmiati
Homeostasis Vol 2, No 1 (2019)
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Abstract: The Intensive Care Unit (ICU) is a treatment room for patients at risk for nosocomial infections. One of transmission for nosocomial infections is through the air. The aim of this study was to identify type of airborne contaminant fungi in the ICU Idaman Banjarbaru Hospital. This study used descriptive method with cross sectional approach. Sampling was done by used the open plate method for 15 minutes at five points in the ICU, where 4 points in each corner and one point in the middle of the room. The media used in this study was SDA + (Saboraud Dextrose Agar + Chloramphenicol 0.05%) as many as 5 media. The results showed that one species of fungus was Aspergillus niger with 5 colonies. The conclusions from this study are obtained one species of airbone contaminant fungi in the ICU room of Idaman Banjarbaru Hospital namely Aspegillus niger. Keywords: air contaminants fungi, ICU, nosocomial infections, Aspergillus niger. Abstrak: Ruang Intensive Care Unit (ICU) merupakan ruang perawatan pasien yang beresiko untuk terjadinya infeksi nosokomial. Salah satu cara penularan infeksi nosokomial adalah melalui udara. Penelitian ini bertujuan untuk mengidentifikasi jenis jamur kontaminan udara di ruang ICU RSD Idaman Banjarbaru. Penelitian ini menggunakan metode deskriptif dengan pendekatan cross sectional . Pengambilan sampel dilakukan dengan menggunakan metode open plate selama 15 menit di lima titik di ruang ICU, dimana 4 titik di masing-masing sudut dan satu titik di tengah ruangan. Media yang digunakan pada penelitian ini adalah SDA+(Saboraud Dextrose Agar + Kloramfenikol 0,05%) sebanyak 5 media Hasil penelitian didapatkan satu spesies jamur yaitu Aspergillus niger dengan jumlah koloni 5. Simpulan dari penelitian ini adalah didapatkan satu spesies jamur kontaminan udara di ruang ICU RSD Idaman Banjarbaru yaitu Aspergillus niger. Kata-kata kunci: jamur kontaminan udara, ICU, infeksi nosokomial, Aspergillus niger
Perbandingan Aktivitas Antibakteri Ekstrak Daun Tanjung dan Daun Jambu Biji terhadap Escherichia coli In Vitro Rinny Vebliani; Noor Muthmainah; Alfi Yasmina
Homeostasis Vol 3, No 1 (2020)
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Abstract

Abstract: Tanjung leaves and guava leaves are commonly used by the community as herbal medicines. Guava leaves contain antibacterial compounds, namely, saponins, tannins, alkaloids and flavonoids, while the tanjung leaves contain alkaloids, tannins and saponins. This study aimed to examine the comparison of the antibacterial activity between tanjung leaves extract and guava leaves extract against Escherichia coli in vitro. The methods used in this study was true experimental with post test only with control group design, consisting of 10 treatments of tanjung and guava leaves extracts at the concentrations of 20%, 25%, 30% and 35%, chloramphenicol as positive control, and distilled water as negative control. The parameter measured was the diameter of the inhibitory zone. Data analysis used the Kruskal-Wallis test and the Mann-Whitney test, with 95% confidence level. In this study, the results obtained showed no significant differences between treatments with tanjung leaves extract and guava leaves extracts at the concentrations of 20%, 25%, 30%, and 35% (p > 0.05). The conclusion of this study is that the antibacterial activity of tanjung leaves extract did not differ significantly with guava leaves extract against E. coli at the same concentration. Keywords: Escherichia coli, inhibitoryzone, tanjung leaves, and guava leaves  Abstrak: Daun tanjung dan daun jambu biji biasa digunakan oleh masyarakat sebagai obat herbal. Daun jambu mengandung zat antibakteri yaitu saponin, tanin, alkaloiddanflavonoid, sedangkan pada daun tanjung yaitu alkaloid, tanindansaponin. Penelitian ini bertujuan untuk mengetahui perbandingan aktivitas antibakteri ekstrak daun tanjung dan daun jambu biji terhadap Escherichia coliin vitro. Metode penelitian ini adalah true experimental dengan rancangan post test only with control grup design, terdiri dari 10 perlakuan ekstrak daun tanjung dan daun jambu biji dengan konsentrasi 20%, 25%, 30% dan 35%, kloramfenikol sebagai kontrol positif, serta akuades sebagai kontrol negatif. Parameter yang diukur adalah diameter zona hambat. Analisis data dilakukan dengan menggunakan uji Kruskal-Wallis dan uji Mann-Whitney, dengan tingkat kepercayaan 95%. Pada penelitian ini didapatkan hasil tidak ada perbedaan bermakna antara dari perlakuan ekstrak daun tanjung dan daun jambu biji konsentrasi 20%, 25%, 30%, dan 35% (p > 0,05). Simpulan pada penelitian ini adalah aktivitas antibakteri ekstrak daun tanjung tidakberbeda bermakna secara statistik dibandingkan ekstrak daun jambu biji terhadap E. coli pada konsentrasi yang sama. Kata-kata kunci: Escherichia coli, zona hambat, daun tanjung, daun jambu biji.
Co-Authors Achmad Rofii Agnes Angelina Laila Cibro Agung Biworo Agung Biworo Agung Biworo Agung Biworo Agung Biworo Agung Biworo Alfi Yasmina Alicia Fitri Wulandhany Alvin Saputra Alya Maulida Azma Rosida Dewi Indah Noviana Pratiwi Dewi Indah Noviana Pratiwi Dewi Indah Noviana Pratiwi Dewi Indah Noviana Pratiwi Dhio Husmawan Az’har Dini Rizki Aulia Emilia Zulaiha Erlina Wahyu Elmawati Farida Heriyani Herry Syawali Rabidhamadi Ika Kustiyah Oktaviyanti Irhamna Syari Yani Yani Istiana Istiana Istiana Istiana Khatimah, Husnul Larasati Gilang Puji Astuti Lisda Hayatie, Lisda Meitria Syahadatina Noor Mita Rahma Yani Muhammad Azra Inan Abdillah Muhammad Fajariyanoor Muhammad Syafrie Yunizar Nadita Arianti Sumarno Nani Zaitun Nika Sterina Skripsiana Nosi Kusuma Winarno Putri Nur Qamariah Nur Qamariah Nurhafizah Rafiani Rabidhamadi, Herry Syawali Rahmiati Rahmiati Rahmiati Rahmiati Renny Aditya Renny Aditya Rinny Vebliani Risa Dirgagita Rojwa Hafizhah Roselya Elvaniah Rosmitha Monikayani Sandi Paufik Saputra, Alvin Syaifullah Akbar Pradito Triawanti Triawanti