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All Journal Berkala Kedokteran Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Homeostasis: Jurnal Mahasiswa Pendidikan Dokter
Muthmainah, Noor
Universitas Lambung Mangkurat
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health

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Perbandingan Aktivitas Antibakteri antara Ekstrak Daun dan Kulit Batang Tanjung terhadap Staphylococcus aureus In Vitro Nadita Arianti Sumarno; Alfi Yasmina; Noor Muthmainah
Homeostasis Vol 3, No 1 (2020)
Publisher : Homeostasis

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Abstract: Tanjung (Mimusops elengi L.) is a medicinal plant that can treat various diseases, one of which is caused by Staphylococcus aureus. This plant contains alkaloid, tannin, saponin, and flavonoid which have effect as antibacterials. The purpose of this study was to determine the comparison of antibacterial activity between tanjung leaves and stem bark extracts against S. aureus in vitro. The method of this study was true experimental with post-test only with control group design, consisting of 10 treatments of tanjung leaves and stem bark extracts with concentrations of 15%, 25%, 35%, and 45%, the positive control (clindamycin 2 μg), and the negative control (aquadest). Extraction method used was maceration with 70% ethanol. The positive control was clindamycin 2 μg, and negative control was aquadest. The measured parameter was the diameter of the inhibitory zone. Statistical analysis used were the Kruskal-Wallis test and the Mann-Whitney test, with 95% confidence level. The results of this study found significant differences between treatments of tanjung leaves and stem bark extracts. Significant differences were found between the treatment with tanjung leaves and stem bark extracts of all concentrations, that is, concentrations of 15%, 25%, 35%, and 45%. The conclusion of this study is that there are significant differences in antibacterial activity between tanjung leaves and stem bark extract in the same concentration against S. aureus in vitro. Keywords: Tanjung leaves, tanjung stem bark, Staphylococcus aureus, inhibitory zone Abstrak: Tanaman tanjung (Mimusops elengi L.) adalah tanaman yang memiliki banyak khasiat dalam mengobati berbagai jenis penyakit, salah satunya penyakit yang disebabkan oleh bakteri Staphylococcus aureus. Tanaman ini memiliki kandungan alkaloid, tanin, saponin, dan flavonoid yang bersifat antibakteri. Tujuan penelitian ini adalah mengetahui perbandingan aktivitas antibakteri antara ekstrak daun dan kulit batang tanjung terhadap S. aureus in vitro. Metode penelitian ini adalah true *(experimental dengan rancangan post test-only with control group design, terdiri dari 8 perlakuan ekstrak daun dan kulit batang tanjung dengan konsentrasi 15%, 25%, 35%, dan 45%, serta kontrol positif klindamisin 2 µg, dan kontrol negatif akuades. Metode ekstraksi yang digunakan adalah maserasi dengan pelarut etanol 70%. Kontrol positif klindamisin 2 µg, dan kontrol negatif akuades. Parameter yang diukur adalah diameter zona hambat. Analisis data menggunakan uji Kruskal-Wallis dan uji Mann-Whitney dengan tingkat kepercayaan 95%. Hasil penelitian ini menunjukkan adanya perbedaan bermakna dari perlakuan ekstrak daun dan kulit batang tanjung. Perbedaan yang bermakna terdapat antara perlakuan dengan ekstrak daun dan kulit batang tanjung semua konsentrasi, yaitu konsentrasi 15%, 25%, 35%, dan 45% (p < 0,05). Simpulan pada penelitian ini adalah terdapat perbedaan bermakna aktivitas antibakteri antara ekstrak daun dan kulit batang tanjung pada konsentrasi sama terhadap S. aureus in vitro. Kata-kata kunci: Daun tanjung, kulit batang tanjung, Staphylococcus aureus, Zona hambat
Pola Bakteri Otitis Media Supuratif Kronik Tipe Aman dan Bahaya di RSUD Ulin Banjarmasin Alya Maulida; Achmad Rofii; Noor Muthmainah
Homeostasis Vol 3, No 2 (2020)
Publisher : Homeostasis

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Abstract: Acute otitis media can initiate chronic suppurative otitis media (CSOM) so that the causative bacteria have in common. The accuracy of antibiotic therapy given based on the pattern of bacterial susceptibility in microbiological examination determines the success of CSOM management. The purpose of this study was to determine the bacterial pattern in safe and unsafe type of CSOM in Ulin hospital Banjarmasin. This research is a descriptive study by using accidental sampling method and 33 samples are obtained. The middle ear swab was identified conventionally and planted on growth media using Mac conkey and blood agar media then obtained 26 bacterial isolates. Bacterial pattern in safe type CSOM were shown by descriptive analysis is Staphylococcus aureus (75%), Pseudomonas aeruginosa (12,5%), Staphylococcus epidermidis (12,5%). Meanwhile in unsafe type of CSOM is Staphylococcus aureus (36%), Staphylococcus epidermidis (28%), dan Pseudomonas aeruginosa (8%). Keywords: Chronic suppurative otitis media, CSOM, safe type, unsafe, bacterial pattern Abstrak: Otitis media akut dapat mengawali otitis media supuratif kronik (OMSK) sehingga bakteri penyebabnya memiliki kesamaan. Ketepatan terapi antibiotik yang diberikan berdasarkan pola kepekaan kuman pada pemeriksaan mikrobiologi menentukan keberhasilan penatalaksanaan OMSK. Tujuan penelitian ini adalah untuk mengetahui pola bakteri OMSK tipe aman dan bahaya di RSUD Ulin Banjarmasin. Penelitian ini bersifat dekskriptif dengan menggunakan metode accidental sampling dan didapatkan 33 sampel. Swab telinga tengah diidentifikasi secara konvensional dan ditanam pada media pertumbuhan menggunakan media Mac conkey serta agar darah kemudian didapatkan 26 isolat bakteri. Pola bakteri pada OMSK tipe aman ditunjukkan oleh analisis secara deskriptif yaitu Staphylococcus aureus (75%), Pseudomonas aeruginosa (12,5%), dan Staphylococcus epidermidis (12,5%). Sedangkan pada OMSK tipe bahaya adalah Staphylococcus aureus (36%), Staphylococcus epidermidis (28%), dan Pseudomonas aeruginosa (8%). Kata-kata kunci:  Otitis media supuratif kronik, OMSK, tipe aman, bahaya, pola bakteri
PENGARUH PENYULUHAN TERHADAP PENGETAHUAN DAN SIKAP MENGONSUMSI TABLET TAMBAH DARAH Muhammad Azra Inan Abdillah; Triawanti Triawanti; Azma Rosida; Meitria Syahadatina Noor; Noor Muthmainah
Homeostasis Vol 5, No 3 (2022)
Publisher : Homeostasis

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Iron supplementation tablets (TTD) are dietary supplements containing iron that can be given to adolescent girls to prevent iron deficiency anemia (ADB). Although the administration of TTD in adolescent girls has been implemented, the lack of knowledge and unsupportive attitude toward consuming TTD in adolescent girls can reduce the effectiveness on preventing ADB. This study aims to determine the effect of health education on knowledge and attitude of consuming TTD, a review of adolescent girls at SMP Negeri 25 Banjarmasin. Quasi-experimental research method with one-group pretest-posttest design. The research subjects were 30 people with random sampling technique. Data collection techniques using knowledge and attitude questionnaires. The analysis using the Wilcoxon Signed Ranks test showed that there was a significant difference between knowledge (p=0.00) and attitude (p=0.019) before and after health education for adolescent girls. In conclusion, health education can increase the knowledge and attitude of consuming TTD in adolescent girls
PENGARUH EDUKASI TENTANG PENYAKIT MENULAR SEKSUAL (PMS) TERHADAP PENGETAHUAN DAN SIKAP PELAJAR SMAN 3 BANJARMASIN Dhio Husmawan Az’har; Noor Muthmainah; Nika Sterina Skripsiana; Farida Heriyani; Nani Zaitun
Homeostasis Vol 5, No 3 (2022)
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Sexually Transmitted Diseases (STDs) are infections that are transmitted from one individual to another primarily through sexual contact. The purpose of this study was to determine the effect of Sexually Transmitted Diseases (STD) education on the knowledge and attitudes of SMAN 3 Banjarmasin students. This research design uses a pre-experimental design with one group pretest and posttest design. The sample is 86 people who are students of SMAN 3 Banjarmasin. The research instrument used a questionnaire given before or after education. The sampling technique used was proportional strafied random sampling and analyzed using the Wilcoxon test. The results of this study indicate that there is a significant difference in knowledge and attitudes before (pretest) and after (posttest) education with p = 0.00. It can be concluded that there are differences in the knowledge and attitudes of SMAN 3 Banjarmasin students before and after education
LITERATURE REVIEW: GAMBARAN PEMERIKSAAN TES WIDAL DENGANPEMERIKSAAN PERTUMBUHAN KULTUR BAK-TERI SALMONELLA TYPHI PADA PASIEN DEMAM TIFOID ANAK Sandi Paufik; Noor Muthmainah; Rahmiati Rahmiati; Dewi Indah Noviana Pratiwi; Lisda Hayatie
Homeostasis Vol 5, No 3 (2022)
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Typhoid fever is a systemic contamination because of Salmonella  typhi micro organism, with unfold via the fecal-oral route. Currently, the diagnosis of typhoid fever in Indonesia remains broadly utilized by the Widal take a look at method. From a few data, the sensitivity and specificity values of the Widal take a look at aren't as accurate because the Tubex ™ take a look at. Therefore, this has a look at will investigate a literature assessment that examines the assessment of the Widal take a look at with blood culture in pediatric typhoid fever patients. The layout of this studies is a literature review. Search for posted articles on google schoolar the usage of decided on keywords, namely: evaluate of the Widal take a look at with exam of the boom of Salmonella typhi microorganism in kids with typhoid fever. Based at the assessment results, the av-erage fee of sensitivity is 69.5%, specificity is 67.9%, fantastic predictive fee is 37.9% and poor predictive fee is 86.0%. Widal's exam of the 11 calculated journals has a low common PPV fee and an excessive common NPV fee. Sensitivity and Specificity values are within side the variety of 60%. Conclusion primarily based totally on evaluations from sev-eral journals, the Widal take a look at can not be used to verify a prognosis however to rule out the opportunity of a diagnosis of different diseases
PERBANDINGAN AKTIVITAS ANTIBAKTERI EKSTRAK DAUN SUNGKAI DAN DAUN TANJUNG TERHADAP Salmonella typhi IN VITRO Emilia Zulaiha; Noor Muthmainah; Agung Biworo
Homeostasis Vol 5, No 3 (2022)
Publisher : Homeostasis

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Abstract

Sungkai and Tanjung plants are often used by the community as traditional medicine. Extracts of sungkai leaf and tanjung leaf extract are known to contain active compounds that act as antibacterials such as alkaloids, flavonoids, saponins, tannins and others. This study aimed to analyze the comparison of the antibacterial activity of sungkai and tanjung leaf extracts against Salmonella typhi in vitro. This study was a true experimental study using the Posttest-Only With Control Group Design method with the treatment groups in the form of sungkai leaf extract and tanjung leaf extract with each test concentration of 25%, 50%, 75% and 100% and chloramphenicol 30μg as a positive control and aquadest as a negative control. Data were analyzed using One-Way ANOVA test, Post-hoc LSD and independent T-test. The results of the average diameter of the inhibition zone formed from concentrations of 25%, 50%, 75% and 100% of sungkai leaf extract on the growth of Salmonella typhi were 6.45 mm, 9.20 mm, 11.95 mm, 13.08 mm, respectively. and tanjung leaf extract of 12.70 mm, 15.42 mm, 17.46 mm, 19.20 mm. Based on the results of One-Way ANOVA statistical analysis, the value (p<0.05). The conclusion of this study was that there was a significant difference in the antibacterial activity of the extracts of sungkai and tanjung leaves against Salmonella typhi 
PERBANDINGAN AKTIVITAS ANTIBAKTERI EKSTRAK KULIT BATANG SUNGKAI DAN KULIT BATANG TANJUNG TERHADAP Staphylococcus aureus IN VITRO Nosi Kusuma Winarno Putri; Noor Muthmainah; Agung Biworo
Homeostasis Vol 5, No 3 (2022)
Publisher : Homeostasis

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Abstract

Sungkai and tanjung plants are medicinal plants which used to cure various diseases, including the following diseases that are caused by Staphylococcus aureus. Sungkai bark contains alkaloids, phenols and terpenoids, tanjung bark contains alkaloids, tannins, flavonoid, and saponins which have antibacterial traits. The aim of the study is to compare the antibacterial activity of sungkai and tanjung bark extracts against S. aureus in vitro. True experimental with post-test only, also using control group design is the chosen research method used upon this research. The 8 treatments of sungkai bark extract and tanjung bark extract with concentration of 25%, 50%, 75%, and 100%, clindamycin 2 µg as control positive, and control negative. The method used for extraction was maceration with 70% ethanol as solvents. The parameter measured was the diameter of the resistance. The average results of inhibition zones with a consensus of 25%, 50%, 75%, 100% sungkai bark extract were 8.27 mm, 10.42 mm, 12.08 mm, 15.38 mm, and the tanjung bark were 13.05 mm,. 16.42mm, 19.57mm, 21.20mm. Data analysis used one-way ANOVA, post-hoc LSD and Independent T with 95% accuracy. The study concludes that there were differences that is stastistically signifficant of antibacterial activity between sungkai and tanjung bark extracts against S. aureus in vitro.. 
Antibiotic Susceptibility Pattern of Extended-Spectrum Beta-Lactamase-Producing Klebsiella Pneumoniae and Escherichia Coli Erlina Wahyu Elmawati; Dewi Indah Noviana Pratiwi; Noor Muthmainah; Agung Biworo
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol. 27 No. 3 (2021)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v27i3.1634

Abstract

Extended-Spectrum Beta-Lactamase (ESBL) producing bacteria is a type of resistance that leads to complex management of patients in intensive care due to their resistance to first, second, and third-generation Cephalosporin and monobactam antibiotics. The most ESBL-producing bacteria are found in the family Enterobacteriaceae, especially Klebsiella pneumoniae and Escherichia coli. The purpose of this research was to determine the sensitivity pattern of ESBLproducing bacteria in Intensive Care Units (ICUs) of Ulin Hospital, Banjarmasin, in the period of 2016-2018. This research was a descriptive study with a cross-sectional approach using data from the laboratory medical records of patients with positive ESBL in the ICUs of Ulin Hospital, Banjarmasin, between 2016 and 2018. The research sample was taken by the total sampling method. This research obtained 216 isolates of ESBL-producing bacteria consisting of 155 (71.8%) isolates of Klebsiella pneumoniae and 61 (28.2%) Escherichia coli. It was found that the Cephalosporin antibiotics (Cefazolin, Ceftazidime, Ceftriaxone, and Cefepime) and monobactam antibiotic (Aztreonam) had the lowest sensitivity. Aminoglycoside antibiotics (Amikacin), Carbapenem (Ertapenem and Meropenem), and Tetracycline (Tigesycline) were the most sensitive antibiotics. It was concluded that both Klebsiella pneumoniae and Escherichia coli were the most dominant ESBL-producing bacteria and showed good sensitivity to the Amikacin, Ertapenem, Meropenem, and Tigecycline.
Susceptibility Pattern of ESBL Urine Specimens in Non-Intensive Care Room at Ulin General Hospital Alicia Fitri Wulandhany; Dewi Indah Noviana Pratiwi; Noor Muthmainah; Agung Biworo
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol. 27 No. 3 (2021)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v27i3.1637

Abstract

Beta-lactam antibiotic resistance can occur in ESBL-producing bacteria such as E.coli and K.pneumoniae, which can cause UTI. One of the risk factors for infection is the non-intensive care space density level. The objective of this study was to determine the sensitivity pattern of ESBL-producing bacteria in urine specimens of patients in the non-intensive care of Ulin General Hospital, Banjarmasin, in the period of 2016-2018. A descriptive study with a cross-sectional design using data results of urine culture and antibiotic susceptibility patterns data in non-intensive care patients at Ulin General Hospital from 2016 to 2018. The urine test results showed 96 positive isolates of ESBL-producing bacteria, consisting of ESBL-E.coli (69.8%) and ESBL-K.pneumonia (30.2%). Antibiotics with low sensitivity tests were Penicillin, Cephalosporin, Monobactam, and Penicillin/beta-lactam inhibitor combinations. Contrastingly, antibiotics with high sensitivity were Aminoglycoside, Carbapenem, and Glycylcycline. It was concluded from this study that the ESBL-producing bacteria in urine specimens for non-intensive care patients of Banjarmasin Ulin General Hospital in the period of 2016-2018 showed varying sensitivity to antibiotics
Antibiotics Susceptibility Pattern in Diabetic Ulcer Patients Mita Rahma Yani; Dewi Indah Noviana Pratiwi; Rahmiati Rahmiati; Noor Muthmainah; Alfi Yasmina
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol. 27 No. 2 (2021)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v27i2.1652

Abstract

Diabetic ulcers are a chronic complication of diabetes mellitus and have a high risk of infection. Severe ulcer infections are a significant cause of lower-extremity amputations in addition to trauma. Therefore, therapy for diabetic ulcer infections must be performed immediately. This study aimed to determine the bacterial susceptibility pattern to the antibiotic in diabetic ulcer patients. This study was retrospective observational descriptive by taking the results of swab culture and antibiotic susceptibility patterns data in diabetic ulcer patients at Ulin General Hospital, Banjarmasin, in 2016-2018. The results showed 41 (62.1%) monomicrobial infections and 25 (37.9%) polymicrobial infections. The number of Gram-negative bacilli (57.4%) was higher than Gram-positive cocci (42.6%). The most common bacterial isolates on pus culture were Staphylococcus aureus (26.6%), Klebsiella pneumonia (19.1%), and Escherichia coli (12.8%). Antibiotic susceptibility test results showed that Gram-positive bacteria were sensitive to Tigecycline (100%), Nitrofurantoin (96.9%), and Linezolid (96.8%). Gram-negative bacteria were susceptible to Ertapenem (92.7%), Meropenem, and Amikacin (90.6%). S.aureus isolates were sensitive 100% to Meropenem and Tigecycline. K.peneumoniae and E.coli isolates were susceptible 100% to Meropenem and Amikacin. It was concluded in this study that the prevalence of Gram-negative bacteria in diabetic ulcer infection was higher than Gram-positive bacteria. The most common isolated Gram-negative bacteria were K.pneumoniae and E.coli, while the most common Gram-positive bacteria were S.aureus. The most sensitive antibiotics for K.pneumoniae and E.coli were Meropenem and Amikacin, while the most sensitive antibiotics for S.aureus were Linezolid and Tigecycline.
Co-Authors Achmad Rofii Agnes Angelina Laila Cibro Agung Biworo Agung Biworo Agung Biworo Agung Biworo Agung Biworo Agung Biworo Alfi Yasmina Alicia Fitri Wulandhany Alvin Saputra Alya Maulida Azma Rosida Dewi Indah Noviana Pratiwi Dewi Indah Noviana Pratiwi Dewi Indah Noviana Pratiwi Dewi Indah Noviana Pratiwi Dhio Husmawan Az’har Dini Rizki Aulia Emilia Zulaiha Erlina Wahyu Elmawati Farida Heriyani Herry Syawali Rabidhamadi Ika Kustiyah Oktaviyanti Irhamna Syari Yani Yani Istiana Istiana Istiana Istiana Khatimah, Husnul Larasati Gilang Puji Astuti Lisda Hayatie, Lisda Meitria Syahadatina Noor Mita Rahma Yani Muhammad Azra Inan Abdillah Muhammad Fajariyanoor Muhammad Syafrie Yunizar Nadita Arianti Sumarno Nani Zaitun Nika Sterina Skripsiana Nosi Kusuma Winarno Putri Nur Qamariah Nur Qamariah Nurhafizah Rafiani Rabidhamadi, Herry Syawali Rahmiati Rahmiati Rahmiati Rahmiati Renny Aditya Renny Aditya Rinny Vebliani Risa Dirgagita Rojwa Hafizhah Roselya Elvaniah Rosmitha Monikayani Sandi Paufik Saputra, Alvin Syaifullah Akbar Pradito Triawanti Triawanti