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Deteksi Antibodi dan Isolasi Toxoplasma gondii pada Itik lokal di Bali (DETECTION ANTIBODIES AND ISOLATION OF TOXOPLASMA GONDII IN DOMESTIC DUCK IN BALI ) Made Dwinata; Ida Bagus Made Oka; I Made Damriyasa
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This study was performed to determine detection antibodies and isolation T. gondii infection in domestic duck in Bali. A total, 188 domestic ducks sera were examined using indirect haemaglutination test kit (IHA). Heart, brain and muscle of seropositive IHA test were used for isolation with pepsin-HCL digestion and bioassay in mice and cat. The result of these research showed that 47 (25%) ducks were found to be positive for T. gondii antibodies at the cut-off e” 1:64. The seroprevalence in male and female duck were 27,8% and 22,4% respectively, however, statistical analysis showed that the difference was not significant (P>0,05). The seroprevalence in cage and free-range duck were 18,7% and 29,2% respectively, but the difference was not statistically significant (P>0,05). The antibodies titer ranging from 1:64 to 1:2048. Also, viable T. gondii was isolated from seropositive duck by bioassay mice and cat. Most of the isolated strains were avirulent to mice. This study showed that domestic duck could have a potensial role in transmitting toxoplasmosis to human in Bali.
Studi Patologi Kejadian Cysticercosis pada Tikus Putih I Ketut Berata; Anak Agung Gde Arjana; I Wayan Sudira; I Made Merdana; I Ketut Budiasa; Ida Bagus Made Oka
Jurnal Veteriner Vol 11 No 4 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Rats are commonly used as animal model in pathological and reproduction research, butunfortunately they are often infected with cysticercosis. The objective of this research was to determinethe pathological changes the of the rats (Rattus novergicus) tissues affected with cysticercus. Thisresearch using 24 of female rats. They were adapted to a new environment for a week and the feeding andwater were provided ad libitum. At the end of adaptation period rats were necropsied and the visceralorgans were examined for pathological changes especially the present of cysticercosis. The liver and kidneyof each rat were soaked in 10% phosphate buffered formalin. Following dehydration process, tissue wereembedded in paraplast, cut at 5 micron and stained with Harris hematoxylin eosin (HE). The resultshowed that 8 of 24 rats were affected by cysticercosis on the liver. The histopathological changes werenecrotic lesions and eosinophylic cells infiltration around the cysticercosis lesion. The results showed that8 of 23 rats were affected by cysticercosis. The presence of necrosis and cells inflammation could interferethe results of the study when such a rats are used. It is therefore necessary to screen rats for cysticercosis.
Produksi dan Karakterisasi Antibodi Monoklonal Anti-Cysticercus cellulosae (PRODUCTION AND CHRACTERIZATION OF MONOCLONAL ANTIBODIES AGAINST CYSTICERCUS CELLULOSAE) Ida Bagus Ngurah Swacita; I Made Damriyasa; Nyoman Sadra Dharmawan; Nyoman Mantik Astawa; Ida Ayu Pasti Apsari; Ida Bagus Made Oka; I Wayan Masa Tenaya
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The purpose of this study is to make a monoclonal antibody against- Cysticercus cellulosae and itscharacterization. Samples antigen prepared from T. solium larvae (C. cellulosae) was then used to immunizeBalb/c. The immune response of mice assessed by ELISA test, then the lymphocytes of mice used for theproduction of monoclonal antibodies (MoAb). Origin lymphocytes of mice that produce antibodies againstC. cellulosae antigen, fused with myeloma cells (NS1). Results fusion of two cells produces hybrid cellscalled hybridomas; cells are then screened by ELISA test. Hybridoma cells that produce only MoAb, usedto produce large quantities in vitro. Characterization of MoAb against-C.cellulosae was tested by usingELISA and Western blotting. Mice were immunized with C.cellulosae antigen showed an immune responseproducing antibodies to C.cellulosae. Based on the results of fusion, produced a total of 51 hybridoma cellclones and after being screened, only three clones of hybridoma cells that produced MoAb against–C.cellulosae. MoAb produced, named after the hole where the growth of the ELISA micro plate, the BE6,BE7, and EE9. Characteristics of this MoAb capable of tracking cellulosae of fluid larvae and recognizeantigen protein bands with molecular weight 78kDa.
Identifikasi Jenis Lalat Tidak Penghisap Darah pada Peternakan Babi di Bali Alice Viria Cordeiro da Costa Xavier; I Made Dwinata; Ida Bagus Made Oka
Buletin Veteriner Udayana Vol. 14 No. 5 October 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2022.v14.i05.p13

Abstract

Flies are ectoparasites that act as vectors of disease and their existence greatly affects the health of pigs. The purpose of this study was to identify the types and fluctuations in the number of flies that do not suck blood in pig farms in Badung Regency and Denpasar City. The fly collection was carried out on a semi-intensive pig farm with a period of every week for 3 months. In this study, flies were caught using fly paper glue and sweeping net and then euthanized with 70% alcohol. The data obtained were presented descriptively and analyzed using the Wilcoxon test. The identification results showed that the type of non-blood-sucking flies found in pig farms in Badung Regency and Denpasar City was Musca sp. as many as 2,383 tails (96.99%), Lucilia sp. as 56 tails (2.28%) and Fannia sp. as 18 tails (0.73%). During the 12 weeks of observation, fluctuations occurred with the highest decrease in fly population in the 3rd week (November) and 10th (January) while the increase occurred in the 5th week (December) to the 9th week (January). The number of fly population found in the Denpasar pig farm is 1,464 larger, which is largerthan the population in the pig farm in Badung Regency 993 tails.
Prevalensi Infeksi Cacing Nematoda Gastrointestinal pada Ayam Petelur di Desa Peninjoan, Kecamatan Tembuku, Kabupaten Bangli, Bali Agostinho Moreira Belo; I Nyoman Adi Suratma; Ida Bagus Made Oka
Buletin Veteriner Udayana Vol. 15 No. 1 February 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v01.i01.p03

Abstract

Nematodes are among the most important class of worm parasites in poultry in terms of the number of species and the damage they cause. Common nematode worms that infect the digestive tract of chickens include: Capilaria spp, Tetrameres spp, Aquaria spp, Ascaridia galli, Strongyloides avium, and Heterakis gallinarum. Risk factors that influence the incidence of parasite infection are influenced by; host, parasites and environment. Host factors that most commonly affect the incidence of infection include: type, age, sex, while parasite factors that affect infection include: mode of transmission, viability (survival), pathogenicity and immunogenicity, as well as environmental factors that influence, especially: weather, enclosure sanitation and humidity. The purpose of this study was to determine the prevalence of nematode grastrointestinal infection in laying hens in Tembuku Sub-district, Bangli District, Bali. The object of this study is feces from laying hens taken directly as many as 240 laying hens. The inspection method used is qualitatively using the floating method. The result showed that the prevalence of gastrointestinal nematode worm infection in laying hens was 9,17 %. The result of the data analysis showed that there was no relationship between the age of the laying hens and the prevalence of gastrointestinal nematode worn infection in laying hens reared in Peninjoan village, Tembuku Sub-district, Bangli district, Bali.
Prevalensi dan Identifikasi Ektoparasit pada Anjing Kintamani Bali di Bali Ni Wayan Nur Sidi Murti; Ida Bagus Made Oka; I Made Dwinata
Buletin Veteriner Udayana Vol. 15 No. 2 April 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i02.p17

Abstract

Ectoparasites are parasites that live parasitically on the outside of the host's body or tunnels in the skin that are still in contact with the external environment. Impacts caused by ectoparasite infestations in dogs include decreased body weight, decreased production, hair loss, irritation, anemia and even death. The purpose of this study was to determine the prevalence of infestation and identification of ectoparasites that infest the Anjing Kintamani Bali (AKB). The AKB samples used were 100 individuals. The Anjing Kintamani Bali selected as the sample tested positive if ectoparasites were found in the dog. Identification of ectoparasites based on morphology. The data obtained were analyzed by Chi-square test. The results showed that the prevalence of ectoparasites in AKB in Bali was 56%. The types of ectoparasites that infest include: Riphicephalus sanguinus, Ctenocephalides felis, Heterodoxus spiniger and Trichodectes canis. Chi-square test found that maintenance methods were associated with the prevalence of ectoparasite infestations in AKB in Bali, while age and sex were not correlated. Maintenance of the Anjing Kintamani Bali in Bali, you should pay more attention to animal health. Regular bathing of animals is highly recommended to control ectoparasite infestations, besides that the owner can comb the animals regularly. Sanitation of cages for animals in cages is very important to pay attention to, besides that, it is better for animals that have been severely infested to be taken to the nearest veterinarian.
Prevalensi Infeksi Koksidia pada Anjing Kintamani Bali Luh Gede Winda Maheswari; I Made Dwinata; Ida Bagus Made Oka
Buletin Veteriner Udayana Vol. 15 No. 2 April 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i02.p18

Abstract

Coccidiosis is an infectious disease of the digestive tract in dogs that can cause digestive disorders. The purpose of this study was to determine the prevalence of coccidia infection and its relationship with risk factors (age, sex, maintenance system) in Kintamani Bali Dogs. Sampling was carried out in several areas in Bali, namely Denpasar City, Gianyar Regency and Sukawana Village, Kintamani District, Bangli Regency by purposive sampling. The number of research samples was 150 faecal samples and examined using the floating method. The data obtained were analyzed by chi-square test. The results showed that from 150 samples of Kintamani Bali Dog feces examined, 5 samples were positive for Coccidia infection with a prevalence of 3.33%, 2 samples were identified as infected with Eimeria spp. 1.33% (2/150) and 3 samples infected with Isospora spp. 2% (3/150). Analysis by Chi-square test there was no significant relationship between age, sex and maintenance system on the prevalence of coccidia infection in Kintamani Bali Dogs. The owner of the Kintamani Bali Dog should pay more attention to maintenance, both from feeding, cleaning the cage and also administering protozoan drugs to reduce the prevalence of Coccidia infection.
Prevalensi Infestasi Tungau Kudis pada Anjing di Bali dengan Metode “Tape Strip" Nonitema Nazara; Ida Bagus Made Oka; I Made Dwinata
Buletin Veteriner Udayana Vol. 15 No. 2 April 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i02.p01

Abstract

Mange is a disorder of the skin that can cause displeasure and changes in the skin. Scurvy is caused by Sarcoptes scabiei and Demodex spp. The number of dermatitis events in dogs is caused by maintenance management by removing which may be able to contact with dogs with mange. The purpose of this study was to find out the prevalence of mange mite infestations, the type of mite, and its relationship to risk factors that influence infestations in dogs in Bali. The number of study samples was 100 dogs in Bali that showed clinical symptoms in the form of disobedience which was distinguished by age, race, gender, and way of maintenance. The dog selected as a diselotip sample is then examined with a microscope to identify the mites. A dog sample tests positive if at least one mite is found. The results showed the prevalence of mange mites in dogs in Bali was 22% consisting of Sarcoptes scabiei 10% and Demodex spp. 12%. Further identification found Demodex canis with a prevalence of 7% and Demodex cornei 5%. The Chi-square test found that age and way of maintenance had a prevalence relationship between mite infestations, while gender and race were not associated with the prevalence of mite infestations. In connection with the large prevalence, serious attention is needed for dogs kept in Bali, especially regarding skin hygiene and health.
Prevalensi Infeksi Cacing Toxocara Canis pada Anak Anjing Kintamani di Kabupaten Bangli Bali Ni Nyoman Widiasih; I Made Dwinata; Ida Bagus Made Oka
Buletin Veteriner Udayana Vol. 15 No. 2 April 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i02.p06

Abstract

The kintamani dog is one of the original Indonesian breed dogs that have a lot of fans. This dog comes from the mountainous area of Sukawana Village, Kintamani District, Bali. Toxocariosis is one of the most common diseases infecting puppies. The more interest of this puppies, it affect the way they are kept, which sometimes causes diseases caused by bacteria, viruses and parasites. One of the is T. canis worm can cause inflammation and edema in the liver and lungs puppies. This study aims to determine the prevalence of T. canis infection in kintamani puppies and their relationship with risk factors for dog age. This study is an observational study with a cross-sectional approach. The total sample of this study amounted to 100 samples of feces of kintamani puppies, examined by flotation concentration method using saturated salt solution. The research data is presented descriptively and to determine the relationship between age and prevalence, the chi-square analysis is used. The results showed that the prevalence of T. canis infection in kintamani puppies was 79% (79/100). The results of the study were based on age group: the prevalence of kintamani puppies at 1 month old was 62.5% (15/24), at 2 months the prevalence was 90.3% (28/31), and at 3 months the prevalence was 80.0% (36/45). The results of the chi-square test analysis showed that there was a significant relationship between age risk factors (P<0.05) and the prevalence of T. canis infection. Regular administration of deworming drugs to puppies and pregnant mother dogs 1 to 2 weeks before birth to prevent and reduce transmission of T. canis worm infection through prenatal and transmamari.
Isolasi Jenis Cemaran Parasit pada Tanah Ida Ayu Pasti Apsari; Ida Bagus Ngurah Swacita; Ida Bagus Made Oka
Buletin Veteriner Udayana Vol. 15 No. 4 August 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i04.p23

Abstract

Research has been carried out on the soil around the cowshed and grazing land. Soil is the source of infection/infestation of L3 (larval stage 3), infective eggs, oocysts and mite or ticks. Soil-Transmitted Pathogens are organisms that live on the ground, but in a short time, then the continuity of the cycle requires a host. The cow acts as host in this case. The aim of the study is to isolate the type of parasitic contamination in the soil around the enclosure and grazing land. Each soil sample of 100 is collected from dry calcareous areas (Jimbaran) and the wet regions (Mengwi-Badung). The float method with MgSO4 (English salt) is modified as a method to determine the type of soil contamination. The results of the study showed 45% (90/200) soil contaminated by parasites. The parasite contamination found is coccidia oocyst, protozoa cyst, trematode and nematode worm eggs, nematode worm larvae and mite eggs and larvae. Conclusions from the results are 45% of the soil are contaminated by parasitic namely protozoa, worm eggs, mite larvae and eggs from the Bukit Jimbaran region (29%) and Mengwi (61%).
Co-Authors Ady Fendriyanto, Ady Affan Nur Alamsyah, Affan Nur Agostinho Moreira Belo Agung Mourizd Adventus Bili Bora Agus manahan manik Agustina A Naibaho Aida Lousie Tenden Rompis Aini, Hanifah Alshofa Nurul Alice Viria Cordeiro da Costa Xavier Anak Agung Gde Arjana Anak Agung Gde Oka Dharmayudha Anak Agung Raka Pramasudha Astuti K. W. Ayu Murni Desrini Malelak, Ayu Murni Desrini Ayu Talia Shalsa Billa Budhy Jasa Widyananta Desyandri Desyandri Dharma, I Putu Panji Nara Dharmawan N.S Dhea Septiany Peda Lalupada Febriyani R R Telnoni, Febriyani R R Gilang Kala Maulana, Gilang Kala Glantiga, I Gede Jaya Rama Heri Utomo Baihaqi, Heri Utomo I Gede Mahardika I Gusti Agung Made Armada Hambarsika I K. K. Agustina I Kadek Swastika I Ketut Berata I Ketut Puja I Made Damriyasa I Made Dwinata I Made Kardena I Made Merdana I Made Subrata I NYOMAN ADI SURATMA I NYOMAN MANTIK ASTAWA I Putu Darmadi I Putu Hendra Pradipta I Wayan Masa Tenaya I Wayan Sudira I WAYAN YUSTISIA SEMARARIANA Ida Ayu Pasti Apsari Ida Ayu Pasti Apsari Ida Bagus Ngurah Swacita Ida Bagus Oka Winaya Ida Bagus Windia Adnyana Kadek Ari Dwipayanti Kadek Karang Agustina Ketut Budiasa Ketut Widyani Astuti Lestari, Anak Agung Istri Trisna Jiwani Luh Dewi Anggreni Luh Gede Winda Maheswari Madani, Inggrid Mahatriny N. N. Muhammad Gustav Satriadistfa Septiadi, Muhammad Gustav Muhammad Wilmar Akbar Muhsoni Fadli, Muhsoni Mukti, Taufik Ni Made Ayudiningsih Astiti Sudewi Ni Nyoman Mahatriny, Ni Nyoman Ni Nyoman Widiasih Ni Putu Sanggra Payani, Ni Putu Ni Wayan Nur Sidi Murti Nonitema Nazara Pande Ketut Suwanti Devi, Pande Ketut Payani N. P. S. Putranty, Rahmi Maulidya Putu Anna Oktaviana Putu Titin Evi Sucitrayani Rencong Dwi Putra, Rencong Dwi Rio Fadly Sihombing Saduarsasila, I Gede Agus Kana Sajuri, Indri Agustin Stevi Sam suri Samsuri Samsuri SAMUYUS NEALMA Santri Yulita, Santri Saputri, Megawati Satria, Julian Septianingsih, Ni Luh Putu Diah Sonda, Kristina Sartika Sri Kayati Widyastuti Sumartono - Utama, Kadek Jaya Wayan Tunas Artama Wisana, Kadek Adya Arsa Yoseph, Veronica Vriscilla Zefanya, Fiorencia