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All Journal Buletin Udayana Mengabdi Jurnal Farmasi Udayana Jurnal Veteriner Indonesia Medicus Veterinus Program Kreativitas Mahasiswa - Penelitian Buletin Veteriner Udayana
Ida Bagus Made Oka
Fakultas Kedokteran Hewan Universitas Udayana
Education Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Lymphocytes Subpopulation in Peripheral Blood and Spleen of Village Chickens Recognized by Monoclonal Antibodies (SUBPOPULASI LIMFOSIT PADA DARAH TEPI DAN LIMPA AYAM KAMPUNG YANG DIKENALI OLEH ANTIBODI MONOCLONAL) Nyoman Mantik Astawa; Ida Bagus Made Oka
Jurnal Veteriner Vol 17 No 2 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Lymphocytes play important role in host defence system against pathogenic agents both in mammalianand avian species. Monoclonal antibodies (mAbs) have been widely used to identify lymphocytessubpopulation in a host based on their surface cluster differentiation (CD) markers. Currently, mAbsagainst lymphocytes surface markers of village chickens have been produced by fusion of myeloma withlymphocytes derived from spleen of mice immune to chicken lymphocytes. In two fusion experiments, 623clones of hybridomas were produced and four (BG4, CB1, DB2 and BB2) of which secreted mAbs againstchickens lymphocyte surface molecules. Two mAbs (BG4 and DB2) recognized protein of 32 kDa, one mAb(CB1) recognized protein of 64 kDa, and one mAb was unable to recognize any protein of chicken lymphocytesurface molecule. Three mAbs recognized lymphocyte subpopulation in spleen and peripheral blood ofvillage chickens. In peripheral blood, mAbs BG4, CB1 and DB2 recognized lymphocytes subpopulationwith the percentages of 11.2%, 21.4% and 7.4% respectively. In spleen those three mAbs recognizedlymphocytes subpopulations at the percentages of 38.2%, 51.54% and 31.5% respectively. Based on thoseresult, it is very likely that mAbs BG4 and DB2 recognized CD4 molecule and mAb CB1 recognized CD8molecule of village chickens lymphocytes.
Case of Entamoebiasis in Pigs Raised with a Free Range Systems in Bali, Indonesia (KASUS ENTAMOEBIASIS PADA BABI YANG DIPELIHARA DENGAN CARA DIUMBAR DI BALI, INDONESIA) Kadek Karang Agustina; Anak Agung Gde Oka Dharmayudha; Ida Bagus Made Oka; I Made Dwinata; I Made Kardena; Nyoman Sadra Dharmawan; I Made Damriyasa
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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This research aims were to measure the prevalence of Entamoeba in pigs in Bali and to identify thezoonotic potential species of Entamoeba. A total of 183 pig stool samples from Bali have been examined.The method being used in this study were combination between coproscopy and molecular techniques.Concentration sedimentation with Sodium Acetic Formaldehide (SAF) as a solution was used in thecoproscopy method, while the Polimerase Chain Reaction method was used to amplify DNA of Entamoeba.Extracted sample’s DNA examined by using primers that specifically for Entamoeba: Entam 1 (F) (5’-GTTGAT CCT GCC AGT ATT ATA TG-3’) and Entam 2 (R) (5’-CAC TAT TGG AGC TGG AAT TAC-3), and toidentify the zoonotic potential species of Entamoeba, samples that produce 550 bp in first amplificationcontinued by primers Epolecki1 (F) (5’-TCG ATA TTT ATA TTG ATT CAA ATG-3’) and Epolecki2 (R) (5’-CCT TTC TCC TTT TTT TAT ATT AG-3’). The results showed that 76.6% of samples were positive incoproscopical examination, but 84.7 % produced 550 bp bands on PCR amplification by using generalprimers. All positive samples on the first PCR continued to second PCR used specific primers for E.poleckii as a potential zoonotic disease and all of the samples showed negative results. This datademonstrated that the prevalence of Entamoeba in a traditional pig scavenging systems in Bali was 84.7%but no specific infection infection caused by E. polecki was found.
Pneumonia Verminosa pada Kucing Lokal yang Terinfeksi oleh Aelurotsrongylus sp (VERMINOUS PNEUMONIA IN DOMESTIC CAT INFECTED BY AELUROSTRONGYLUS SP) Ida Bagus Oka Winaya; I Ketut Berata; I Made Kardena; Ida Bagus Made Oka
Jurnal Veteriner Vol 13 No 4 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of the study was to evaluate the pulmo pathological changes of domestic cat infected byAelurostrongylus sp. A total of 15 cats were examined at Faculty of Veterinery Medicine, Udayana Universityduring 2010. Ten out of 15 cats showed sneezing, whereas the remains showed serous rhinitis and sneezing.Macroscopic and microscopic changes were observed mainly on pulmo samples. Hyperemias on caudalislobes and pleura effusion were found in the pulmo. The pulmo tissue was fixed on 10 % neutral bufferformalin and stained with haematoxilin-eosin (HE) for histopathological examination. Aelurostrongylus spwas present in the alveoli lumen of the lung samples. Meanwhile, the alveoli septa of the lung wereobserved thicker and infiltrated with neutrophils, plasma exudates and erythrocytes. Pleural effusion wasmainly consisted of eosinophilic substances. It is concluded that verminous pneumonia in domestic catinfected with Aelurostrongylus sp was an acute infection.
Ovisidal dan Vermisidal Bawang Putih terhadap Telur dan Cacing Ascaridia galli pada Ayam Kampung (OVICIDAL AND VERMICIDAL ACTIVITIES OF GARLIC AGAINST THE EGGS AND ADULT HELMINTH OF ASCARIDIA GALLI IN KAMPONG CHICKENS) Ida Bagus Made Oka
Jurnal Veteriner Vol 4 No 2 (2003)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

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Prevalence of Intestinal Worm in Free Ranging Domestic Cats in Bali (PREVALENSI CACING USUS PADA KUCING PELIHARAAN YANG BEBAS BERKELIARAN DI BALI) I Made Subrata; Ida Bagus Made Oka; Kadek Karang Agustina
Jurnal Veteriner Vol 18 No 3 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (87.504 KB) | DOI: 10.19087/jveteriner.2017.18.3.441

Abstract

The aims of this study were to identify and to measure the prevalence of intestinal worm infections in free-ranging domestic cats in Bali. As many as 133 cat fecal samples were collected from Bali and preserved in sodium acetic formaldehyde solution. Coproscopy method (sedimentation concentration and flotation techniques) was used to identify the eggs of helminthes. Based on fecal examination, eggs of four helminthes species : Toxocara, Ancylostoma, Cestoda and Capillaria were identified. This result indicates the prevalence of intestinal worms in free ranging domestic cats were high, consisting of Toxocara sp (71.43%), Ancylostoma sp (37.59%), Cestoda (19.55%) and Capillaria sp (0.75%). Therefore, it is needed to conduct programs to reduce and eradicate that helminthes. ABSTRAK Penelitian ini bertujuan untuk mengidentifikasi jenis cacing dan mengukur prevalensi infeksi kecacingan pada kucing yang diliarkan di Bali. Sebanyak 133 sampel feses kucing yang berasal dari seluruh Bali dikumpulkan dan disimpan dalam larutan sodium acetic formaldehide. Seluruh sampel diperiksa secara koproskopi dengan dua metode berbeda yaitu metode konsentrasi sedimentasi dan metode pengapungan untuk mengidentifikasi telur cacing yang terdapat pada feses kucing. Pada penelitian ini teridentifikasi empat jenis cacing yang menginfeksi kucing yang diliarkan di Bali yaitu Toxocara, Ancylostoma, Cestoda dan Capillaria. Hasil penelitian ini menunjukkan bahwa prevealensi infeksi kecacingan pada kucing yang diliarkan di Bali masih tinggi, yaitu Toxocara sp (71,43%), Ancylostoma sp (37,59%), Cestoda (19,55%) dan Capillaria sp (0,75%). Untuk itu diperlukan program pemberantasan dan pencegahan terhadap infeksi kecacingan pada kucing di Bali.
Imunitas Protektif Mencit Terhadap Cairan Kista Taenia saginata (PROTECTIVE IMMUNITY OF MICE AGAINST CYST FLUID OF TAENIA SAGINATA) Nyoman Sadra Dharmawan; I Made Dwinata; I Made Damriyasa; Ida Bagus Made Oka; Kadek Swastika; Luh Dewi Anggreni; Nyoman Mantik Astawa
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of this research was to determine immune response of mice against vaccines derived fromcyst fluid of Taenia saginata. The study was conducted using four BALB/c mice aged 6 weeks as experimentalanimals. All experimental animals were vaccinated intra peritoneal with Taenia saginata cyst fluidemulsified in Freund’s adjuvant. Immune response in the mice was determined by detecting antibodiesusing ELISA and by the presence of lymphocytes through evaluation of blood smear. The results showedthat the cyst fluid of Taenia saginata was antigenic and capable of inducing antibody responses that weredetected by ELISA. Mean antibody titers obtained in the results of the first, second, third, and fourth ofvaccination was 3.3 units; 17.9 units; 21.2 units; and 72.1 units; respectively. Evaluation of blood smear ofvaccinated mice showed an increase in the percentage of lymphocytes after vaccination with an average66.75%, compared with the average of lymphocytes before vaccination which was 40.75%. Further researchis still required in experimental animals by vaccination followed by challenge test with Taenia saginataeggs.
Deteksi Antibodi dan Isolasi Toxoplasma gondii pada Itik lokal di Bali (DETECTION ANTIBODIES AND ISOLATION OF TOXOPLASMA GONDII IN DOMESTIC DUCK IN BALI ) Made Dwinata; Ida Bagus Made Oka; I Made Damriyasa
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This study was performed to determine detection antibodies and isolation T. gondii infection in domestic duck in Bali. A total, 188 domestic ducks sera were examined using indirect haemaglutination test kit (IHA). Heart, brain and muscle of seropositive IHA test were used for isolation with pepsin-HCL digestion and bioassay in mice and cat. The result of these research showed that 47 (25%) ducks were found to be positive for T. gondii antibodies at the cut-off e” 1:64. The seroprevalence in male and female duck were 27,8% and 22,4% respectively, however, statistical analysis showed that the difference was not significant (P>0,05). The seroprevalence in cage and free-range duck were 18,7% and 29,2% respectively, but the difference was not statistically significant (P>0,05). The antibodies titer ranging from 1:64 to 1:2048. Also, viable T. gondii was isolated from seropositive duck by bioassay mice and cat. Most of the isolated strains were avirulent to mice. This study showed that domestic duck could have a potensial role in transmitting toxoplasmosis to human in Bali.
Studi Patologi Kejadian Cysticercosis pada Tikus Putih I Ketut Berata; Anak Agung Gde Arjana; I Wayan Sudira; I Made Merdana; I Ketut Budiasa; Ida Bagus Made Oka
Jurnal Veteriner Vol 11 No 4 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Rats are commonly used as animal model in pathological and reproduction research, butunfortunately they are often infected with cysticercosis. The objective of this research was to determinethe pathological changes the of the rats (Rattus novergicus) tissues affected with cysticercus. Thisresearch using 24 of female rats. They were adapted to a new environment for a week and the feeding andwater were provided ad libitum. At the end of adaptation period rats were necropsied and the visceralorgans were examined for pathological changes especially the present of cysticercosis. The liver and kidneyof each rat were soaked in 10% phosphate buffered formalin. Following dehydration process, tissue wereembedded in paraplast, cut at 5 micron and stained with Harris hematoxylin eosin (HE). The resultshowed that 8 of 24 rats were affected by cysticercosis on the liver. The histopathological changes werenecrotic lesions and eosinophylic cells infiltration around the cysticercosis lesion. The results showed that8 of 23 rats were affected by cysticercosis. The presence of necrosis and cells inflammation could interferethe results of the study when such a rats are used. It is therefore necessary to screen rats for cysticercosis.
Produksi dan Karakterisasi Antibodi Monoklonal Anti-Cysticercus cellulosae (PRODUCTION AND CHRACTERIZATION OF MONOCLONAL ANTIBODIES AGAINST CYSTICERCUS CELLULOSAE) Ida Bagus Ngurah Swacita; I Made Damriyasa; Nyoman Sadra Dharmawan; Nyoman Mantik Astawa; Ida Ayu Pasti Apsari; Ida Bagus Made Oka; I Wayan Masa Tenaya
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The purpose of this study is to make a monoclonal antibody against- Cysticercus cellulosae and itscharacterization. Samples antigen prepared from T. solium larvae (C. cellulosae) was then used to immunizeBalb/c. The immune response of mice assessed by ELISA test, then the lymphocytes of mice used for theproduction of monoclonal antibodies (MoAb). Origin lymphocytes of mice that produce antibodies againstC. cellulosae antigen, fused with myeloma cells (NS1). Results fusion of two cells produces hybrid cellscalled hybridomas; cells are then screened by ELISA test. Hybridoma cells that produce only MoAb, usedto produce large quantities in vitro. Characterization of MoAb against-C.cellulosae was tested by usingELISA and Western blotting. Mice were immunized with C.cellulosae antigen showed an immune responseproducing antibodies to C.cellulosae. Based on the results of fusion, produced a total of 51 hybridoma cellclones and after being screened, only three clones of hybridoma cells that produced MoAb against–C.cellulosae. MoAb produced, named after the hole where the growth of the ELISA micro plate, the BE6,BE7, and EE9. Characteristics of this MoAb capable of tracking cellulosae of fluid larvae and recognizeantigen protein bands with molecular weight 78kDa.
THE PREVALENCE AND INTENSITY OF EIMERIA SPP INFECTION IN ETAWAH CROSSBREED GOAT IN SUKAWATI SUB-DISTRICT, GIANYAR BALI I Dewa Ayu Diah Pradnya Pramita; Ida Ayu Pasti Apsari; Ida Bagus Made Oka; Romy Muhammad Dary Mufa
Buletin Veteriner Udayana Bul. Vet. Udayana. April 2024 Vol. 16 No. 2
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2024.v16.i02.p10

Abstract

Etawah Crossbreed Goat are in demand by farmers because of the variety of products they produce, such as milk, meat, and fur. Coccidiosis is a disease that frequently infects goats in Indonesia and is influenced by host, agent and environment. This study aimed to determine the prevalence and intensity of Eimeria spp infection in etawah crossbreed goat kept in Sukawati District, Gianyar Regency. The number of samples used in this study was 100 feces samples of etawah crossbreed goats in Sukawati District, Gianyar Regency. The design used in this study was cross sectional study. There are two methods of examination carried out, namely qualitative and quantitative methods of examining fecal samples. In qualitative examination using the floating method then the results were analyzed with the chi-square test and quantitative examination using the McMaster method. The results of this study showed that there were 79 samples infected with Eimeria spp. with a prevalence of 79%. The average intensity of Eimeria spp. infection in PE goats in Sukawati District is 740.35 ± 557.05 oocysts/gram. After analysis using chi-square test, it can be concluded that risk factors were not associated with the prevalence of Eimeria spp. infection in PE goats in Sukawati District, Gianyar Regency. Considering the high prevalence of Eimeria spp. infection in PE goats in Sukawati District, Gianyar Regency, it is recommended that farmers increase their concern for cage sanitation and the environment around the cage and routine drug administration.
Co-Authors Ady Fendriyanto, Ady Affan Nur Alamsyah, Affan Nur Agostinho Moreira Belo Agung Mourizd Adventus Bili Bora Agus manahan manik Agustina A Naibaho Aida Lousie Tenden Rompis Aini, Hanifah Alshofa Nurul Alice Viria Cordeiro da Costa Xavier Anak Agung Ayu Mirah Adi Anak Agung Gde Arjana Anak Agung Gde Oka Dharmayudha Anak Agung Raka Pramasudha Astuti K. W. Ayu Murni Desrini Malelak, Ayu Murni Desrini Ayu Talia Shalsa Billa Baiq Intan Rizqi Amalia Budhy Jasa Widyananta Desyandri Desyandri Dharma, I Putu Panji Nara Dharmawan N.S Dhea Septiany Peda Lalupada Febriyani R R Telnoni, Febriyani R R Gilang Kala Maulana, Gilang Kala Glantiga, I Gede Jaya Rama Heri Utomo Baihaqi, Heri Utomo I Dewa Ayu Diah Pradnya Pramita I Gede Mahardika I Gusti Agung Made Armada Hambarsika I Gusti Ketut Suarjana I K. K. Agustina I Kadek Swastika I Ketut Berata I Ketut Puja I Made Damriyasa I Made Dwinata I Made Kardena I Made Merdana I Made Subrata I Nengah Kerta Besung I NYOMAN ADI SURATMA I Nyoman Mantik Astawa I NYOMAN MANTIK ASTAWA I Putu Darmadi I Putu Hendra Pradipta I Wayan Masa Tenaya I Wayan Sudira I WAYAN YUSTISIA SEMARARIANA Ida Ayu Pasti Apsari Ida Ayu Pasti Apsari Ida Ayu Pasti Apsari Ida Bagus Kade Suardana Ida Bagus Ngurah Swacita Ida Bagus Oka Winaya Ida Bagus Windia Adnyana Kadek Ari Dwipayanti Kadek Karang Agustina Ketut Budiasa Ketut Widyani Astuti Komang Ayu Triana Sanjiwani Lestari, Anak Agung Istri Trisna Jiwani Luh Dewi Anggreni Luh Gede Winda Maheswari Madani, Inggrid Mahatriny N. N. Muhammad Gustav Satriadistfa Septiadi, Muhammad Gustav Muhammad Wilmar Akbar Muhsoni Fadli, Muhsoni Mukti, Taufik Ni Made Ayudiningsih Astiti Sudewi Ni Nyoman Mahatriny, Ni Nyoman Ni Nyoman Widiasih Ni Putu Sanggra Payani, Ni Putu Ni Wayan Nur Sidi Murti Nonitema Nazara Pande Ketut Suwanti Devi, Pande Ketut Payani N. P. S. Putranty, Rahmi Maulidya Putu Anna Oktaviana Putu Titin Evi Sucitrayani Rencong Dwi Putra, Rencong Dwi Rio Fadly Sihombing Romy Muhammad Dary Mufa Saduarsasila, I Gede Agus Kana Sajuri, Indri Agustin Stevi Sam suri Samsuri Samsuri SAMUYUS NEALMA Santri Yulita, Santri Saputri, Megawati Satria, Julian Septianingsih, Ni Luh Putu Diah Sonda, Kristina Sartika Sri Kayati Widyastuti Sumartono - Utama, Kadek Jaya Wayan Tunas Artama Wisana, Kadek Adya Arsa Yolla Noviolita Yoseph, Veronica Vriscilla Zefanya, Fiorencia