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All Journal International Journal of Public Health Science (IJPHS) SAINS MEDIKA : JURNAL KEDOKTERAN DAN KESEHATAN Jurnal Kedokteran Diponegoro Journal of Nutrition College Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Journal of Biomedicine and Translational Research Mutiara Medika: Jurnal Kedokteran dan Kesehatan The Indonesian Biomedical Journal Paediatrica Indonesiana Jurnal Gizi dan Dietetik Indonesia (Indonesian Journal of Nutrition and Dietetics) Bioscientia Medicina : Journal of Biomedicine and Translational Research Medica Hospitalia Bioscientia Medicina : Journal of Biomedicine and Translational Research Life Science
Nani Maharani
Department Of Pharmacology And Therapy, Faculty Of Medicine, Diponegoro University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health Other

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Effects of fermentation duration on α-glucosidase activity and vitamin C in temu mangga kombucha Zebua, Edarni; Maharani, Nani; Anjani, Gemala; Rustanti, Ninik; Al-Baari, Ahmad Ni’matullah
Jurnal Gizi dan Dietetik Indonesia (Indonesian Journal of Nutrition and Dietetics) VOLUME 13 ISSUE 6, 2025
Publisher : Alma Ata University Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21927/ijnd.2025.13(6).444-454

Abstract

ABSTRAKLatar Belakang: Diabetes Mellitus Tipe 2 (DMT2) merupakan gangguan metabolik yang semakin meningkat di Indonesia, ditandai dengan tingginya kadar gula darah akibat gangguan fungsi sel β pankreas. Pangan fungsional yang memiliki sifat antioksidan dan kemampuan menghambat enzim pencernaan karbohidrat, seperti kombucha, berpotensi mendukung pengelolaan DMT2.  Tujuan: Menganalisis aktivitas inhibisi enzim α-glukosidase dan kadar vitamin C pada kombucha temu mangga.  Metode: Penelitian eksperimental dilakukan di UPT Laboratorium Terpadu, Universitas Diponegoro, Semarang, mulai Agustus sampai November 2024, menggunakan rancangan acak lengkap dengan empat lama fermentasi (5, 7, 10, dan 14 hari), masing-masing diulang tiga kali. Aktivitas penghambatan enzim α-glukosidase dan kadar vitamin C dianalisis menggunakan spektrofotometri UV-Vis pada panjang gelombang 540 nm dan 265 nm. Data aktivitas inhibisi α-glukosidase dan kadar vitamin C dianalisis dengan ANOVA, jika hasil uji menunjukkan perbedaan signifikan (p<0,05) dilanjutkan uji Post Hoc Tukey untuk menentukan kelompok yang berbeda dan Kruskal Wallis digunakan jika data tidak berdistribusi normal.Hasil: Aktivitas penghambatan enzim α-glukosidase tertinggi diperoleh pada hari ke-14 fermentasi (75,84 ± 1,46%), menunjukkan penurunan signifikan dibandingkan kontrol (87,42 ± 0,96%). Kandungan vitamin C tertinggi ditemukan pada hari ke-5 fermentasi (2,06 ± 0,06 mg/100 mL), kemudian menurun seiring waktu fermentasi. Kesimpulan: Kombucha temu mangga menunjukkan potensi sebagai minuman fungsional yang mampu menghambat enzim α-glukosidase secara in vitro dan mengandung antioksidan yang relevan. Penelitian lanjutan diperlukan untuk menguji efektivitas dan keamanannya secara klinis pada manusia. KATA KUNCI : diabetes melitus; enzim α-glukosidase; kombucha; temu mangga (curcuma mangga); vitamin c  ABSTRACTBackground: Type 2 Diabetes Mellitus (T2DM) is a growing metabolic disorder in Indonesia, associated with impaired pancreatic β-cell function and elevated blood glucose levels. Functional foods with antioxidant and enzyme-inhibitory properties, like kombucha, may support T2DM management.Objective: To analyze the inhibition activity of the α-glucosidase enzyme and the vitamin C content in temu mangga kombucha.Methods: An experimental study was conducted in UPT Laboratory, Diponegoro University, Semarang, from August to November 2024., using a completely randomized design with four fermentation durations (5, 7, 10, and 14 days), each in triplicate. α-Glucosidase inhibition and vitamin C levels were assessed using UV-Vis spectrophotometry at wavelengths of 540 nm and 265 nm. The inhibition activity data of α-glucosidase and vitamin C levels were analyzed using ANOVA. If the test results showed significant differences (p<0.05), a Tukey Post Hoc test was conducted to determine the different groups, and Kruskal-Wallis was used if the data were not normally distributed.Results: The highest α-glucosidase inhibitory activity was observed on day 14 (75.84 ± 1.46%), showing a significant reduction compared to the control (87.42 ± 0.96%). The highest vitamin C content (2.06 ± 0.06 mg/100 mL) was detected on day 5, followed by a gradual decline with prolonged fermentation.Conclusion: Temu mangga kombucha exhibits promising in vitro α-glucosidase inhibitory potential and relevant antioxidant content. These findings suggest its potential as a dietary adjunct for T2DM management. However, in vivo studies are warranted to confirm its efficacy and safety in human subjects. KEYWORDS : α-glucosidase enzyme; diabetes mellitus; kombucha; temu mangga (curcuma mangga); vitamin cArticle submitted on January 31, 2025; Articles revised on February 20, 2025; Articles received on June 12, 2025; Articles available online on November 28, 2025
Co-Authors Abraham Talent Bawadi Sibarani Agustini Utari Aisy, Rihadatul Al-Baari, Ahmad Ni’matullah Amin Husni Anjani, Gemala Ardyarini, Hikmatunnisa Tri Asri, Hairul Astika Widy Utomo, Astika Widy Awal Prasetyo Bahrudin Besari Adi Pramono Budiono, Bernardus Parish Cecilia Noviyanti Salim Dzaki Edmonda Setyawan Edarni Zebua Edi Dharmana Endang Mahati Endang Mahati Farmaditya EP Mundhofir Ferdy Kurniawan Cayami Ferry Sandra Gatot Irawan Sarosa, Gatot Irawan Hery Djagat Purnomo Hesti Triwahyu Hutami Hisatome, Ichiro Ika Pawitra Miranti Indriyati, Rita Intan Rahmania Eka Dini Jaeri, Santoso Julian Dewantiningrum Kuwabara, Masanari Li, Peili M Ali Sobirin Manda Petrina Martin Adhitya Subagio Meita Hendrianingtyas Muhammad Thifan Satyagraha Muniroh, Muflihatul Neni Susilaningsih Ninik Rustanti Ninomiya, Haruaki Nugraha, Eka Djatnika Pratama, Reza Dian Qolby, Qonita Nur Qorina Putri Srisantoso Rahajeng, Herera Rayvita AN Meagratia Rebriarina Hapsari Retnaningsih Retnaningsih Reza Dian Pratama Rizky Aditya Fardhani Ryco Giftyan Ardika Selamat Budijitno Sihombing, Nydia Rena Benita Sultana MH Faradz Surya Utoyo, Frederick Taufiq, Fikri Tri Indah Winarni Udadi Sadhana, Udadi Udin Bahrudin Udin Bahrudin, Udin Wibowo, Erwin Widiastiti, Nyoman Suci Widyastuti, Esti Wiwik Lestari Yan Wisnu Prajoko Yora Nindita Yuriz Bakhtiar