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Viogenta, Pratika
Prodi Farmasi FMIPA, Universitas Lambung Mangkurat, BanjarBaru
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Bioautography and FTIR Analysis of Ethanol Fraction Morel Berry Root (Physalis angulata L.) Against Staphylococcus epidermidis and Pseudomonas aeruginosa Pratika Viogenta; Latifah Megasari; Laila Susanti
Majalah Obat Tradisional Vol 27, No 3 (2022)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.78171

Abstract

Morel berry root contains flavonoids, tannins, and alkaloids that have an antibacterial characteristics. This study aims to determine the antibacterial activity of the morel berry root ethanol fraction against Staphylococcus epidermidis and Pseudomonas aeruginosa and the most effective active compounds to inhibit such activity. Extractions of morel berry root used the maceration method with ethanol 70% of and then continued by fractionation using ethanol, chloroform, and n-hexane. Test of the compound fraction was performed using Thin Layer Chromatography (TLC) and characterization with FTIR Spectrophotometer. The results of the antibacterial activity with inhibitory zone diameter were at a 100% concentration of 18.69 mm in S. epidermidis while P. aeruginosa of 20.00 mm. Bioautographic results for ethanol fraction indicate an inhibited zone with an Rf value of 0.72 in the S. epidermidis and P. aeruginosa which is thought to be a flavonoid compound. Characterization with FTIR spectrophotometer functional groups O-H phenol, C = C aromatic, C-H aromatic, C-O alcohol, and C-H aliphatic in ethanol fraction with Rf 0.72.
Antibacterial Activity and Bioautography of the Chloroform Fraction of Morel Berry (Physalis angulata L.) Root Against Staphylococcus epidermidis and Pseudomonas aeruginosa Pratika Viogenta; Laila Susanti; Latifah Megasari
Journal of Multidisciplinary Applied Natural Science Vol. 3 No. 1 (2023): Journal of Multidisciplinary Applied Natural Science
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jmans.2774-3047.148

Abstract

The urgency of finding novel sources of active compounds to overcome infectious diseases is encouraged. Morel berry (Physalis angulata L.) is a traditional herbal plant that can be used as an antimicrobial because of its unique chemical content. This study aims to find compounds that have antibacterial activity from the chloroform fraction of morel berry roots. This study used the Kirby-Bauer method with concentrations of 20%, 40%, 60%, 80%, and 100% against Staphylococcus epidermidis and Pseudomonas aeruginosa bacteria. Determination of antibacterial compounds and their functional groups was performed using thin-layer chromatography (TLC) technique and reagent spray test, TLC-Bioautography and Fourier transforms infrared (FTIR) studies. The chloroform fraction of morel berry showed a zone of inhibition with the highest diameter of 11.50 and 12.00 mm against S. epidermidis and P. aeruginosa, respectively. Phytochemical screening revealed the presence of alkaloids, flavonoids, tannins, and saponins in the chloroform fraction. The relative retention stain of 0.54 on the TLC plate inhibited the growth of the two tested bacteria and indicated the presence of functional groups O-H phenol, C-H aliphatic, C=O ester, C=C aromatic, C-OH alcohol, and C-H aromatic. This study found that tannins in the roots of morel berries could be used as a natural antibacterial agent to treat S. epidermidis and P. aeruginosa.
Pelatihan Penjernihan dan Pemanfaatan Minyak Jelantah Menjadi Lilin Aroma Terapi di Guntung Paikat, Banjarbaru Selatan, Kalimantan Selatan Pratika Viogenta; Sutomo Sutomo; Normaidah Normaidah
Jurnal Pengabdian ILUNG (Inovasi Lahan Basah Unggul) Vol 2, No 3 (2023)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/ilung.v2i3.6462

Abstract

Pemahaman akan pengolahan limbah di masyarakat dilakukan untuk meminimalisir pencemaran lingkungan. Salah satu limbah rumah tangga di daerah Guntung Paikat, Banjarbaru Selatan, Banjarbaru yakni adalah limbah minyak jelantah, dikarenakan banyaknya warga yang berprofesi sebagai penjual kuliner baik katering, ayam geprek dan yang lainnya, ditambah lagi minyak jelantah yang dihasilkan dari rumah tangga. Berdasarkan permasalahan tersebut, tim pengabdian kepada masyarakat (PkM) Program Studi Farmasi Universitas Lambung Mangkurat berinisiatif untuk melaksanakan suatu pelatihan pemanfaatan limbah minyak jelantah menjadi lilin aroma terapi. Tim bekerja sama dengan Kelompok PKK. Kegiatan ini dilaksanakan pada tanggal 13 Agustus 2022 dengan peserta yang hadir sebanyak 18 orang. Kegiatan tersebut terdiri dari: penyuluhan dampak dan bahaya penggunaan minyak goreng berulang-ulang, pelatihan penjernihan minyak jelantah dengan menggunakan kombinasi arang aktif kelapa sawit dan bleaching earth, demonstrasi pembuatan lilin aroma terapi berbahan dasar minyak jelantah serta evaluasi peragaan pembuatan lilin aroma terapi berbasis minyak jelantah dari para peserta pelatihan. Luaran yang dihasilkan berupa teknik pengelolaan limbah minyak jelantah, dan produk lilin aroma terapi. Secara keseluruhan kegiatan pelatihan pembuatan lilin aroma terapi berbasis minyak jelantah dan sosialisasi pentingnya mengurangi penggunaan minyak goreng berulang-ulang berjalan dengan sukses.
Studi Fitokimia Jamur Endofit Tumbuhan Seluang Belum (Luvunga sarmentosa (Blume) Kurz) Asal Kabupaten Tabalong Kalsel Nashrul Wathan; Pratika Viogenta; Jehan Azizah; Fery Ramadhan; Sindwi Rinanda Sari
Jurnal Pharmascience Vol 10, No 1 (2023): Jurnal Pharmascience
Publisher : Program Studi Farmasi FMIPA Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jps.v10i1.14317

Abstract

Seluang belum adalah tumbuhan asal Kalimantan yang dipercaya oleh masyarakat sekitar sebagai afrodisiaka karena memiliki metabolit sekunder yang bervariasi. Endofit adalah mikroba yang hidup di dalam jaringan tumbuhan dan mampu menghasilkan metabolit sekunder yang serupa dengan inangnya. Tujuan dari penelitian ini adalah mendapatkan isolat jamur endofit dari akar Seluang belum (Luvunga sarmentosa (Blume) Kurz.) dan melakukan skrining fitokimia senyawa yang dikandungnya. Proses penumbuhan isolat jamur endofit dari akar seluang belum (L. sarmentosa) dilakukan pada media Potato Dextrose Agar (PDA) hingga didapatkan isolat terpisah. Jamur endofit murni yang dihasilkan kemudian diamati identitasnya secara makroskopik dan mikroskopik. Jamur endofit hasil isolasi lalu dibiakkan dalam media Potato Dextrose Broth (PDB), senyawa bioaktif yang terkandung lalu diekstraksi menggunakan etil asetat dan dilanjutkan identifikasi senyawa fitokimianya. Penelitian ini mendapatkan 6 isolat endofit yang diidentifikasi sebagai Rhizoctonia solani, Corynespora citricola Ellis, Arthrobotrys oligospora Fresenius, Alternaria Nees:Fr., Phytophthora capsici dan Tripospermum Speg. Kandungan senyawa fitokimia yang diskrining dari ekstrak etil asetat keenam jamur endofit hasilnya beberapa isolat positif mengandung golongan senyawa fenolik, flavonoid, steroid, dan terpenoid. Kata Kunci: Saluang Bilung, lavanga sarmentosa, Fungi Endofit, Herba Kalimantan, Suku Dayak   Seluang belum. is a plant from Kalimantan which commonly believes by local people as an aphrodisiac because it has various secondary metabolites. Endophytes are microbes that live in plant tissues and are able to produce secondary metabolites similar to their host. The purpose of this study is to isolating the endophytic fungus from Seluang belum (Luvunga sarmentosa (Blume) Kurz) root and to perform phytochemical screening of its metabolites. Endophytic fungi isolated from seluang belum roots then grown in PDA (Potato Dextrose Agar) media until obtained separate isolates. The obtained fungal isolates then identified macroscopically and microscopically to determine their identity. The isolated fungi then fermented in PDB (Potato Dextrose Broth) media, the secondary metabolites then extracted with ethyl acetate and identified its phytochemicals. The results showed that 6 endophytic isolates were identified as Rhizoctonia solani, Corynespora citricola Ellis, Arthrobotrys oligospora Fresenius, Alternaria Nees:Fr., Phytophthora capsici and Tripospermum Speg. The phytochemical content that screened from the ethyl acetate extract of 6 endophytic fungi was resulting some positive containing groups of phenolic compounds, flavonoids, steroids, and terpenoids.
Cox2 Inhibitor Test Of Limpasu (Baccaurea Lanceolata) Using Computation Method Samsul Hadi; Nashrul Wathan; Deni Setiawan; Pratika Viogenta; Kunti Nastiti
Journal of Midwifery and Nursing Vol. 5 No. 3 (2023): September: Health Science
Publisher : Institute Of Computer Science (IOCS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35335/jmn.v5i3.4104

Abstract

The use of anti-inflammatory drugs with the COX-2 method is starting to develop tolerance, so that drugs circulating in the community now cause various kinds of complaints, namely inhibition of blood clotting and stomach ulcers. Therefore, the purpose of this study was to screen natural product compounds that have potential as COX-2 inhibitors. The materials used are compounds contained in B.lanceolata and COX-2 enzymes with code 3ln1 obtained from the RCSB database, the equipment used is a windows laptop with PLANT and discover studio software, the method used in this study is a computational study using docking software . The results in this study were that the docking score of B. lanceolata was higher than the native ligand, so the effectiveness was weaker than the native ligand, the docking scores obtained were as follows: Epidihydrotutin (-50.243); Sapidolide A (-51052); Melatonin (-53039); 6'-O-Vanilloylisotachioside (-60.910) and 6'-O-Vanilloyltachioside (-61.131). In conclusion, 6'-O-Vanilloyltachioside is a potential compound as a COX-2 inhibitor from B.lanceolata.
Peel-off Kefir Mask Arachi (Arachis hypogaea L.): Characterization and Antioxidant Activity Amalia Khairunnisa; Pratika Viogenta; Nani Kartinah; Fathur Rahman; Mulia Mulia
Borneo Journal of Pharmacy Vol. 5 No. 1 (2022): Borneo Journal of Pharmacy
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjop.v5i1.2597

Abstract

This study aims to determine the best formulation for the peel-off mask Arachi or peanut (Arachis hypogaea L). Arachis hypogaea kefir as an active ingredient is added with variations in the concentration of F1 (0.5%) and F2 (2%) (w/v). Organoleptic tests, homogeneity, dry time, and pH were carried out on the peel-off mask that had been made. Antioxidant test (DPPH methods) was performed on masks F1 and F2. The results showed that the peel-off mask of A. hypogaea kefir had the best antioxidant activity at a concentration of 2% (F2) kefir with an IC50 value of 1.865 ppm and was very active. The characteristics of the peel-off mask have good physical stability, proven by not experiencing a change in color, odor, being homogeneous, having good dispersion power, and having a dry time ranging from 10-23 minutes. The pH value of the peel-off mask preparation is 4.52, and it is appropriate with SNI and the pH balance of normal human skin. The peel-off mask of A. hypogaea kefir can be produced because has good physical stability and antioxidant activity.
Edukasi Penerapan Teknologi Fermentasi melalui Pembuatan Kefir dan Yogurt Amalia Khairunnisa; Pratika Viogenta; Nani Kartinah; Arnida Arnida; Sutomo Sutomo; Fadlilaturrahmah Fadlilaturrahmah
Jurnal Nusantara Berbakti Vol. 2 No. 4 (2024): Oktober : Jurnal Nusantara Berbakti
Publisher : Universitas Kristen Indonesia Toraja

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59024/jnb.v2i3.411

Abstract

Fermentation technology can increase added value to agricultural, food and other products. Through fermentation, raw materials can be converted into products with higher selling value, such as cheese, yogurt, tempeh, wine, beer, and so on. Many fermented products are beneficial for health, for example kefir and yogurt which contain good probiotics. for digestion. Kefir is a type of fermented milk whose manufacture uses various microorganisms to produce lactic acid products with by-products such as ethanol, free fatty acids and acetaldehyde. Kefir is made by adding yeast which contains many microorganisms from the lactic acid bacteria and yeast groups, while yogurt is fermented milk with lactic acid bacteria (LAB).  This service activity aims to increase people' knowledge and skills regarding the application of fermentation technology, starting from the meaning of fermentation, fermentation methods to high-value products produced from the fermentation process. In this activity, socialization activities were carried out regarding the development of fermentation technology, especially kefir and cow's milk yogurt, as well as practices on how to make it among the residents of Balitra Jaya Permai Loktabat Utara. The results show an increase in residents' knowledge and skills regarding fermentation through pretest and posttest activities as well as practical activities.
Pemberian Makanan Tambahan Melalui Olahan Jamu Dan Nugget Kelor Sebagai Upaya Pencegahan Stunting Amalia Khairunnisa; Sutomo Sutomo; Nani Kartinah; Pratika Viogenta; Rizqan Azhari
SAFARI :Jurnal Pengabdian Masyarakat Indonesia Vol. 3 No. 4 (2023): Oktober: Jurnal Pengabdian Masyarakat Indonesia
Publisher : BADAN PENERBIT STIEPARI PRESS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56910/safari.v3i4.850

Abstract

Stunting is a state of malnutrition and causes delays in brain development and child development in the first 1000 days of life. One of the factors that causes stunting is a lack of nutritional intake in infants or toddler mothers. The purpose of this service is to provide education and practice regarding supplementary feeding through moringa leaves such as nuggets and moringa herbs in Mandiangin Barat village, South Kalimantan. The method used in this service activity is in the counseling about the use of Moringa leaves as processed nutritious food and drinks, as well as the practice of processing Moringa nuggets and herbs. The results of these activities can be concluded that the community in Mandiangin Barat village has developed a level of understanding about supplementary feeding and can process into food and drinks that have nutritional value as an effort to prevent stunting rates in the village.
Program Wadai Bingka (Wadah Kami Belajar Meningkatkan Kesehatan): Dampak Pernikahan Dini, Kesehatan Reproduksi Dan Alat Kontrasepsi Nani Kartinah; Pratika Viogenta; Sutomo Sutomo; Amalia Khairunnisa; Rezki Agustina
SAFARI :Jurnal Pengabdian Masyarakat Indonesia Vol. 3 No. 4 (2023): Oktober: Jurnal Pengabdian Masyarakat Indonesia
Publisher : BADAN PENERBIT STIEPARI PRESS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56910/safari.v3i4.919

Abstract

The negative impact of early marriage is the increased risk of maternal and child mortality, miscarriage, stunting in children, and poor reproductive health conditions. Wadai Bingka activities aim to increase public knowledge about the right marriage age, the importance of maintaining reproductive health and the use of contraceptives. The method used is in the form of education using an extension system, booklets, teaching aids and animated videos. There were 24 participants. Evaluation of activities is carried out by comparing the score values obtained by participants before the implementation of the activity (pre-test) and after the implementation of the activity (posttest). Based on the evaluation results, changes in participants' knowledge for good criteria increased by 84%, medium criteria decreased by 54%, and bad criteria decreased by 100%. It can be concluded that Wadai Bingka's service activities have a good impact as evidenced by an increase in community knowledge.
Antioxidant Assay of Kefir Peanut (Arachis hypogaea L.) with Variations in Concentration and Fermentation Time Viogenta, Pratika; Khairunnisa, Amalia; Kartinah, Nani; Rahmadati, Erfiza
Majalah Obat Tradisional Vol 29, No 3 (2024)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.87814

Abstract

Kefir is a processed beverage produced through pasteurized milk fermentation using a starter from kefir grains. These grains contain groups of lactic acid bacteria and yeast. Peanuts can be used as a raw material for kefir products and Arachis hypogaea L. has antioxidant activity due to a high nutritional content namely vegetable oil, protein, minerals, and essential fatty acids. This study aimed to determine the highest antioxidant activity with variations in concentration and time fermentation of peanut kefir (Arachis hypogaea L.). This research on the antioxidant activity test method used the DPPH method with UV-Vis spectrophotometry. The results of testing the antioxidant activity of peanut kefir with variations in the concentration of kefir grains of 0%, 1%, 2%, and 3% had IC50 values of 64.929 ppm, 57.675 ppm, 54.742 ppm, and 51.870 ppm, respectively. The one-way ANOVA test showed a significant difference between the IC50 value and the concentration of kefir grains. The highest antioxidant in 3% concentration determined the fermentation time with 0, 12, 24, 36, 48, and 60 hours variations. The results of testing the antioxidant activity of kefir peanut with variations in the fermentation time it was had IC50 values of 64.319 ppm respectively, 62.609 ppm, 59.376 ppm, 56.321 ppm, 51.870 ppm, and 51.384 ppm. The one-way ANOVA test results showed no significance in the 0 with 12 hours completion time and 48 with 60 hours. The research results on peanut kefir show that kefir grains of 3% and the duration of fermentation for 48 hours have strong antioxidant activity.
Co-Authors Ahmad Fajri Ahmad Ferry Yeriza Utama Amalia Khairunnisa Amalia Khairunnisa Amalia Khairunnisa, Amalia Arnida Arnida Arnida Arnida Chatarina Umbul Wahyuni Deni Setiawan Desy Aulia Azzahra Fadlilaturrahmah Fadlilaturrahmah, Fadlilaturrahmah Fathur Rahman Fathur Rahman Fery Ramadhan Fitri Fitri Ika Harum Saputri Jehan Azizah Kunti Nastiti Latifah Megasari Latifah Megasari Lilik Koernia Wahidah Mulia Mulia Nani Kartinah Nashrul Wathan Nopiyansyah, Nopiyansyah Normaidah, Normaidah Rahmadati, Erfiza Rezki Agustina Rizqan Azhari Samsuar User Samsuar, Samsuar Samsul Hadi Sindwi Rinanda Sari Siti Nurjanah sunardi sunardi Susanti, Laila Sutomo Sutomo Sutomo Sutomo Sutomo Sutomo Sutomo Sutomo Utama, Ahmad Ferry Yeriza Wathan, Nasrul Yuli Wahyu Tri Mulyani