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All Journal AL KAUNIYAH Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Indonesian Journal of Forestry Research BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Jurnal Bioteknologi & Biosains Indonesia (JBBI) Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology ANNALES BOGORIENSES Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Jurnal Riset Biologi dan Aplikasinya Jurnal Ilmiah Matematika Makara Journal of Science Makara Journal of Technology Annales Bogorienses Berita Biologi
WIBOWO MANGUNWARDOYO
Departemen Biologi FMIPA-UI
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Electrical & Electronics Engineering Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology

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TRANFORMASI FRAGMEN DNA KROMOSOM Xanthomonas campestris KE DALAM Escherichia coli Mangunwardoyo, Wibowo
Makara Journal of Science Vol. 6, No. 1
Publisher : UI Scholars Hub

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Abstract

SELEKSI DAN PENGUJIAN AKTIVITAS ENZIM L-HISTIDINE DECARBOXYLASE DARI BAKTERI PEMBENTUK HISTAMIN Mangunwardoyo, Wibowo; Sophia, Romauli Aya; Heruwati, Endang Sri
Makara Journal of Science Vol. 11, No. 2
Publisher : UI Scholars Hub

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Abstract

Selection and test of L-histidine decarboxylase enzyme activity of six isolates of histamine forming bacteria. Six isolates of histamine forming bacteria were screened to see the degree of ability in producing histamine on modified Niven’s medium. The result showed that the six bacteria were able to produce histamine by giving a pinkish color on the medium, which could be used as a preliminary identification of histamine-forming bacteria (HFB). The isolates were grown in liquid modified Niven medium to measure the production of histamine. The histamine produced were determined by Hardy and Smith method. The result showed that all of the isolates produced high level of histamine (92.35 – 305.49 mg/100 ml of the medium). From all of them, Enterobacter spp. produced the highest level of histamine (305.49 mg/100 ml). A synthetic medium was used to measure the growth pattern and optimum time required by Enterobacter spp and Morganella morganii (as control bacteria) to produce the L-histidine decarboxylase enzyme (HDC) which is responsible for histamine production. The result showed that for both bacteria, the optimum enzim production was 8 hours after incubation.
Beta-Glucosidase 1 (bgl1) Gene Analysis in Mutant and Wild-type of Penicillium sp. ID10-T065 Syafriana, Vilya; Nuswantara, Sukma; Mangunwardoyo, Wibowo; Lisdiyanti, Puspita
Jurnal Riset Biologi dan Aplikasinya Vol. 4 No. 1 (2022)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v4n1.p1-8

Abstract

In the previous study, Penicillium sp. ID10-T065 has been mutated using ultraviolet (UV), ethyl methyl sulfonate (EMS) and the combination of UV-EMS to increase Beta-glucosidase (bgl) activity. There were three mutants selected, UV13 (UV mutant), EM31 (EMS mutant), and UM23 (UV-EMS mutant). This study examined the mutations in the bgl gene encoding (bgl1) as well as sequence differences between mutants and wild-type of Penicillium sp. ID10-T065. The gene analysis was performed by PCR amplification and sequencing of the bgl1 gene. The results of bgl1 gene sequences (600 bp) from mutants were aligned with the wild-type, it was discovered that there were base alterations from position 2025 to 2050. Mutant UV13 showed the highest base alterations (7 bases) which occurred at position 2027 (T?C); 2036 (T?G); 2040 (T?G); 2047 (G?C); and 2048-2050 (TTG?GGA). Mutant EM31 showed alterations in five bases at positions 2034 (G?A), 2036 (T?G), 2037 (G?C), 2044 (G?C), and 2047 (G?T). Mutant UM23 showed two base alterations at position 2025 (T?A) and 2037 (G?C). UV irradiation and EMS mutation resulted in transition and transversion DNA, whereas the combination of UV-EMS mutation resulted in transversion mutations. Base alterations in UV13 and EM31 mutants, causing missense and silent mutation, while in UM23 mutant only silent mutations occur. The bgl1 gene analysis showed that mutation using UV light was more effective than using EMS or a combination of UV-EMS in Penicillium sp. ID10-T065.
Methane Gas Production In Buffalo Rumen Fluid Containing Citronella Residue (Cymbopogon nardus L.) by In Vitro Method Sari, Arina Findo; Mangunwardoyo, Wibowo; Setiawan, Fery Hadi; Sugoro, Irawan
Al-Kauniyah: Jurnal Biologi Vol. 18 No. 2 (2025): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v18i2.40935

Abstract

Methane is one of the greenhouse gases produced by ruminants. One way to reduce methane is by feeding development strategies. Concentrates or forage are two possible forms of animal feed. Forage can be replaced with citronella (Cymbopogon nardus L.) residue, which has not been fully consumed as feed. The purpose of the study was to decide the production of methane gas that has residual citronella to concentrate in buffalo rumen fluid in vitro, for 48 hours. The treatments in this research were 0.4 g citronella residue (A); 0.4 g citronella residue + 0.01 g concentrate (B); 0.4 g citronella residue + 0.02 g concentrate (C); 0.01 g concentrate (D); and 0.02 g concentrate (E). For the 48-hour measurements, the highest methane gas production was treatment 98.2% (D); followed by 92.06% (E); 17.71% (C); 15.33% (A); and 13.54% (B). It can be concluded that methane gas can be reduced by residue citronella. This shows that citronella residue can lower methane gas. The study's findings are anticipated to be among the references for using agricultural product residues, particularly citronella as animal feed to reduce the methane produced by the livestock industry
Analysis of diabetes mellitus gene expression data using two-phase biclustering method Kafi, Rahmat Al; Bustamam, Alhadi; Mangunwardoyo, Wibowo
Jurnal Ilmiah Matematika Vol 8, No 2 (2021)
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26555/konvergensi.v0i0.22111

Abstract

The purpose of this research is to find bicluster from Type 2 Diabetes Mellitus genes expression data which samples are obese and lean people using two-phase biclustering. The first step is to use Singular Value Decomposition to decompose matrix gene expression data into gene and condition based matrices. The second step is to use K-means to cluster gene and condition based matrices, forming several clusters from each matrix. Furthermore, the silhouette method is applied to determine the number of optimum clusters and measure the accuracy of grouping results. Based on the experimental results, Type 2 Diabetes Mellitus dataset with 668 selected genes produced optimal biclusters, with six biclusters. The obtained biclusters consist of 2 clusters on the gene-based matrix and 3 clusters on the sample-based matrix with silhouette values, respectively, are 0.7361615 and 0.7050163.
Diversity and Antimicrobial Activity of Lichens-Associated Actinomycetes in Cibinong Science Centre (CSC) and Cibodas Botanical Garden (CBG) Indonesia Susanti, Agustina Eko; Ratnakomala, Shanti; Mangunwardoyo, Wibowo; Lisdiyanti, Puspita
Annales Bogorienses Vol. 23 No. 1 (2019): Annales Bogorienses
Publisher : BRIN

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Abstract

Bioprospecting has developed to all biological taxa including procaryotic. Actinomycetes become interesting procaryotic because of the ability to produce important secondary metabolite for human life. Actinomycetes are known as the largest antibiotic producer that has a broad range habitat. Some research has been done to find new antibiotic from the various habitat of actinomycetes. One of the interesting habitats of actinomycetes which never been explored in Indonesia is lichens... Lichens as the symbiotic structure of alga and fungi areknown as the ecological niche of various kinds of microorganisms including actinomycetes. Cibinong Science Centre (CSC) and Cibodas Botanical Garden (CBG) have various species of trees as the habitat of lichens. These areas are known as one of the research locations to explore the biodiversity of Indonesia. The aims of this research is to study the diversity and antimicrobial potency of actinomycetes isolated from 10 lichen samples with various type of thallus; crustose, fructose and foliose. Lichen samples were grown on the bark of 9 trees species in CSC and CBG. Isolation process used three agar media; HV, YIM6 and YIM711 with cycloheximide and nalidixic acid. Molecular identification based on 16S rRNA gene sequence. Antimicrobial activity was tested to 65 isolates by agar diffusion method to Bacillus subtilis BTCC B.612, Escherichia coli BTCC B.614, Candida albicans BTCC Y.33, Staphylococcus aureus BTCC B.611, Micrococcus luteus BTCC B.552. Isolation process retrieved 125 isolates with the highest number grow on HV agar medium. Based on the sample, the highest number of actinomycetes were isolated from crustose lichen attached on the bark of Averrhoea carambola. A total 69 isolates were identified as the genera Actinoplanes, Amycolatopsis, Angustibacter, Kribbella, Micromonospora, Mycobacterium, and Streptomyces. The screening process showed 24 isolates have antimicrobial activity, with the highest inhibitory activity against Micrococcus luteus BTCC B.552.
Enhancement of β-Glucosidase Activity in Penicillium sp. by Random Mutation with Ultraviolet and Ethyl Methyl Sulfonate Syafriana, Vilya; Nuswantara, Sukma; Mangunwardoyo, Wibowo; Lisdiyanti, Puspita
Annales Bogorienses Vol. 18 No. 2 (2014): Annales Bogorienses
Publisher : BRIN

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Abstract

The genus Penicillium has a potential ability to produce β-glucosidase. The aim of the study was to improve the β-glucosidase activity of Penicillium sp. ID10-T065 with physical (Ultraviolet = UV), chemical (Ethyl Methyl Sulfonate = EMS), and combined mutation (UV-EMS). The spores of Penicillium sp. ID10-T065 were exposed into UV irradiation for 3 minutes with dose of 0.1 J/cm2 and 13 cm of distances. Chemical mutation was done by treated spores into 3% of EMS solution for an hour. Combined mutation of UV and EMS were also performed by UV for 3 minutes (0.1 J/cm2, 15 cm) and continued with soaking into 2-3% of EMS solution. The developed mutants were screened, selected and assayed. Comparison of enzyme activities with the wild- type (1.78 U/ml), mutant UV13 (5.53 U/ml) showed a 3.1 fold increase; mutant EM31 (4.26 U/ml) showed a 2.4 fold increase. Meanwhile, mutant UM23 obtained from the multiple exposures showed a decreased activity (1.75 U/ml). Mutant UV13 showed the best enzyme activity to be considered as a potential strain for β-glucosidase producer. This result needs to be further elaborated especially on its genetic stability studies in order for the ascertained as a stable mutant.
PENGARUH METODE STERILISASI MEDIA NURSERI DAN ASOSIASI FUNGI MIKORIZA ARBUSKULAR TERHADAP PERTUMBUHAN TANAMAN CABAI (Capsicum annuum L.) Pratama, Aulia Brellian; Mangunwardoyo, Wibowo; Chandra, Nicholas Dwi; Napitupulu, Toga Pangihotan; Idris, Idris; Kanti, Atit; Ikhwani, Azra Zahrah Nadirah; Guswenrivo, Ikhsan; Sudiana, I Made
Berita Biologi Vol 22 No 1 (2023): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/beritabiologi.2023.804

Abstract

Kondisi lingkungan yang kurang baik untuk industri pertanian cabai (Capsicum annuum L.) membuat hasil produksi cabai menurun. Pemberian inokulum mikoriza arbuskular dan pupuk dapat menjadi solusi untuk meningkatkan produksi cabai. Penelitian bertujuan untuk mengetahui pengaruh metode sterilisasi serta interaksi fungi mikoriza arbuskular terhadap pertumbuhan tanaman cabai. Metode penelitian dilakukan dengan melakukan penghitungan jumlah spora, sterilisasi media tanam, penyemaian benih, pengamatan karakteristik agronomi, hingga pengujian infeksi mikoriza. Spora mikoriza arbuskular yang digunakan berupa produk kultur tunggal Glomus mosseae (A) dan kultur campuran (B). Hasil penelitian menunjukkan penghitungan jumlah spora produk B memiliki jumlah spora tertinggi dengan 51 spora/50 g dibandingkan dengan produk A. Analisis karakteristik agronomi dengan ANOVA dua faktor (α: 0,05) menunjukkan tidak adanya pengaruh signifikan antara metode sterilisasi dengan pemberian inokulum mikoriza arbuskular pada tinggi tanaman cabai. Namun, hasil statistik menunjukkan bahwa pemberian mikoriza A dan B memberikan pengaruh terhadap tinggi tanaman cabai. Hasil analisis statistik pengukuran panjang akar juga tidak ada pengaruh signifikan antara pemberian mikoriza dengan metode sterilisasi. Pengamatan infeksi tanaman cabai menunjukkan fungi mikoriza arbuskular kultur tunggal dan campuran mampu menginfeksi akar dengan membentuk hifa internal dan spora. Hasil yang diperoleh dapat digunakan untuk menentukan media tanam serta inokulum mikoriza arbuskular terbaik untuk meningkatkan produksi cabai.
Co-Authors Abinawanto Abinawanto Achmad Dinoto Aisyah, Arina Andi Salamah ARIYANTI OETARI Atit Kanti Bustamam, Alhadi Caniago, Asnany Caterina A, Ines Irene Chandra, Nicholas Dwi Desyandri Desyandri Ekowati Chasanah Endang Sri Heruwati Endang Sukara Fitri, Reno Gintung Patantis Guswenrivo, Ikhsan I Made Sudiana Idris Idris Ifah Munifah Ikhwani, Azra Zahrah Nadirah INDRAWATI GANDJAR Indriawan, Aris Indriawan, Aris Irawan Sugoro Kafi, Rahmat Al Lily Ismaini Lusini, Yuyun Napitupulu, Toga Pangihotan Pratama, Aulia Brellian Pratitis, Asri PUSPITA LISDIYANTI Sari, Arina Findo Setiawan, Fery Hadi SHANTI RATNAKOMALA Siswodarsono, Trismilah Siswodarsono, Trismilah Sita Heris Anita Sophia, Romauli Aya Srikandace, Yoice Suhendar, Dadang Suhendar, Dadang Sukma Nuswantara Sulistiani ., Sulistiani Sulistiani Sulistiani, Sulistiani Susanti, Agustina Eko Syafriana, Vilya Trismilah Trismilah YOPI YOPI Yuliar Yuliar Yusro Nuri Fawzya