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All Journal AL KAUNIYAH Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Indonesian Journal of Forestry Research BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Jurnal Bioteknologi & Biosains Indonesia (JBBI) Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology ANNALES BOGORIENSES Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Jurnal Riset Biologi dan Aplikasinya Jurnal Ilmiah Matematika Makara Journal of Science Makara Journal of Technology Annales Bogorienses Berita Biologi
WIBOWO MANGUNWARDOYO
Departemen Biologi FMIPA-UI
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Electrical & Electronics Engineering Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology

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Penghambatan Enzim L-Histidine Decarboxylase dari Bakteri Pembentuk Histamin Menggunakan Asam Benzoat Heruwati, Endang Sri; Mangunwardoyo, Wibowo
Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 3, No 2 (2008): Desember 2008
Publisher : Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jpbkp.v3i2.14

Abstract

ABSTRAKStudi tentang penghambatan enzim L-Histidine Decarboxylase (HDC) menggunakan asambenzoat telah dilakukan. Dalam percobaan ini, enzim HDC diproduksi dari isolat A4, yangdiidentifikasi sebagai Enterobacter sp. Asam benzoat pada konsentrasi 10, 15, 20, 25, dan 30mM ditambahkan pada ekstrak enzim kasar dan perubahan aktivitas enzim diamati. Hasilpercobaan menunjukkan bahwa semua perlakuan konsentrasi asam benzoat dapat menghambataktivitas enzim HDC, namun penghambatan tertinggi dicapai pada perlakuan 15 mM, denganaktivitas sebesar 0,17 U. Penghambatan aktivitas enzim optimum pada penambahan 15 mMasam benzoat adalah pada suhu 40oC dan pH 6,0. Penambahan ion Fe2+memberikanpenghambatan yang lebih tinggi dibandingkan ion logam lain. Hasil aplikasi perendaman ikantongkol lisong (Euthynnus affinis) dalam larutan asam benzoat 0,1% selama 30 menitmenunjukkan adanya pengaruh nyata terhadap penghambatan pertumbuhan bakteri penghasilhistamin maupun produksi histaminnya, sementara tidak terlihat pengaruh terhadap kadar airmaupun pH ikan.
Produksi Senyawa Bioaktif dari Aspergillus ustus MFW 26-08 yang Berasosiasi dengan Spons Laut dalam Berbagai Media Pratitis, Asri; Patantis, Gintung; Mangunwardoyo, Wibowo; Chasanah, Ekowati
Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 5, No 2 (2010): Desember 2010
Publisher : Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jpbkp.v5i2.411

Abstract

Penggunaan mikroba sebagai sumber senyawa bioaktif memiliki beberapa kelebihan di antaranya mempersingkat waktu produksi dan menghindari pemanfaatan sumberdaya laut secara berlebih. Penelitian terdahulu menghasilkan beberapa isolat mikroba yang berpotensi sebagai penghasil senyawa bioaktif, di antaranya adalah isolat kapang MFW 26-08. Penelitian ini bertujuan untuk melakukan optimasi pertumbuhan isolat kapang MFW 26-08 dan produksi senyawa bioaktif yang disekresikan oleh kapang tersebut. Optimasi dilakukan dengan menggunakan 3 jenis media: Malt Extract Broth (MEB), Glucose Peptone Yeast (GPY), dan Minimal Fungal Media(MFM); serta waktu kultivasi 2, 4, 6, 8, dan 10 minggu. Hasil riset menunjukkan bahwa ekstrak kasar kapang MFW 26-08 hasil kultivasi 2 minggu dalam medium MFM, pada konsentrasi 30 µg/mL, mampu menghambat 89% pertumbuhan sel kanker payudara T47D. Sedangkan pada konsentrasi 100 µg/mL ekstrak kapang yang dikultivasi dalam medium MEB selama 6 minggu, mampu menghambat pembentukan radikal bebas sampai 56%. Hasil identifikasi berdasarkan sifat-sifat morfologi dan molekuler menggunakan 18S rRNA, ITS1, dan ITS4 menunjukkan bahwa isolat MFW 26-08 memiliki kemiripan dengan Aspergillus ustussebesar 99%
Produksi dan Karakterisasi Xilanase dari Isolat Bakteri M-13.2a Asal Air Laut Manado Fawzya, Yusro Nuri; Mangunwardoyo, Wibowo; Munifah, Ifah; Patantis, Gintung
Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 8, No 1 (2013): Juni 2013
Publisher : Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jpbkp.v8i1.53

Abstract

Isolat bakteri M-13.2A yang berasal dari laut Manado diketahui mampu menghasilkan enzim selulase dan xilanase, berdasarkan pembentukan zona bening pada media padat. Penelitian ini bertujuan untuk mendapatkan informasi lebih lanjut mengenai produksi dan sifat enzim xilanase yang dihasilkan dari isolat bakteri M-13.2A serta identifikasi isolat bakteri tersebut di atas. Sebanyak (2,4-3,3) x 108 cfu/ml inokulum dengan konsentrasi sekitar 9% (v/v) diinokulasikan dalam medium xylan broth, kemudian diinkubasi selama 6 hari pada suhu 30°C, 150 rpm. Pengambilan sampel dilakukan setiap hari dan enzim yang dihasilkan diuji aktivitasnya dengan metode asam dinitro salisilat (DNS). Hasil penelitian menunjukkan bahwa aktivitas xilanase tertinggi dihasilkan pada hari ke-2 inkubasi, sebesar 5,17 U/ml. Enzim xilanase ini bekerja optimum pada pH 8, suhu 70°C. Penambahan ion logam 10 mM memberikan pengaruh yang bervariasi terhadap aktivitas enzim. Ion Zn2+ meningkatkan aktivitas xilanase hingga 278,1%. Ion Fe3+ dan Ca2+ menurunkan aktivitas xilanase menjadi 75 dan 8,3% relatif terhadap kontrol, sedangkan ion K+ tidak memberikan pengaruh terhadap aktivitas xilanase. Hasil identifikasi bakteri menunjukkan bahwa isolat M-13.2A memiliki kemiripan 99% dengan Acinetobacter baumannii.
Karakterisasi, Pengaruh Sumber Nitrogen dan Karbon terhadap Produktivitas Enzim Lipase Rhizopus microsporus var oligosporus UICC 550 Mangunwardoyo, Wibowo; Lusini, Yuyun; Gandjar, Indrawati
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 2 (2009): June 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (166.947 KB) | DOI: 10.24002/biota.v14i2.2689

Abstract

Effect of nitrogen and carbon source on the production of extracellular lipase by R.microsporus var. oligosporus UICC 550 was studied. The enzyme activity was alsocharacterized in terms of temperature, pH, stability at room temperature, and effectof divalent ion. The amount of 2.5% (w/v) of olive oil as carbon sources and 5% (w/v)peptone as nitrogen sources were the optimum for production of lipase enzyme.Partial purifications using ammonium sulfate followed by dialysis showed that at pH6.5 and temperature 35oC was the optimum condition, respectively. The stability(remaining up to 80% of the optimum enzyme) was recorded at pH 6.5 after 24 hoursincubation at room temperature. The optimum activity remained 40% of the after onehour incubation at 35oC. Divalent ions concentration at 1 mM, Zn2+, Cu2+, Mg2+ andFe2+ inhibited the lipolytic activity.
Penapisan Aktivitas Selulase Isolat-isolat Khamir dari Moluska, Serasah, dan Tumbuhan di Taman Nasional Gunung Halimun, Jawa Barat Mangunwardoyo, Wibowo; Fitri, Reno; Oetari, Ariyanti
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 3 (2007): October 2007
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (297.223 KB) | DOI: 10.24002/biota.v12i3.2805

Abstract

A total of 236 yeast isolates from mollusc, litter, and plant samples from Gunung Halimun National Park were screened for cellulolytic activity based on Smith method by using 0,2% (w/v) cellulose-azure for 30 days. The results showed that 12 isolates (9 isolates from plants, 2 isolates from molluscs, dan 1 isolate from litter) have cellulolytic activity. These isolates were further screened based on Teather and Wood method for six days to determine their cellulases components by using specific substrates. Carboxymethyl cellulose (CMC) 0,1% (w/v) was used as a specific substrate to determine endoglucanase activity. Avicel 0,1% (w/v) was used as a specific substrate to determine exoglucanase activity. Cellobiose 0,1% (w/v) was used as a specific substrate to determine β-glucosidase activity. The results showed that 6 isolates from plant have β-glucosidase activity, and 1 isolate from plant have β-glucosidase and endoglucanase activities. Five isolates (2 isolates from plants, 2 isolates from molluscs, and 1 isolate from litter) showed no cellulase activity on specific substrates after six days incubation.
Diversity and Antimicrobial Activity of Lichens-Associated Actinomycetes in Cibinong Science Centre (CSC) and Cibodas Botanical Garden (CBG) Indonesia Susanti, Agustina Eko; Ratnakomala, Shanti; Mangunwardoyo, Wibowo; Lisdiyanti, Puspita
ANNALES BOGORIENSES Vol 23, No 1 (2019): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2019.v23.n1.%p

Abstract

    Bioprospecting has developed to all biological taxa including procaryotic. Actinomycetes become interesting procaryotic because of the ability to produce important secondary metabolite for human life. Actinomycetes are known as the largest antibiotic producer that has a broad range habitat. Some research has been done to find new antibiotic from the various habitat of actinomycetes. One of the interesting habitats of actinomycetes which never been explored in Indonesia is lichens... Lichens as the symbiotic structure of alga and fungi areknown as the ecological niche of various kinds of microorganisms including actinomycetes. Cibinong Science Centre (CSC) and Cibodas Botanical Garden (CBG) have various species of trees as the habitat of lichens. These areas are known as one of the research locations to explore the biodiversity of Indonesia. The aims of this research is to study the diversity and antimicrobial potency of actinomycetes isolated from 10 lichen samples with various type of thallus; crustose, fructose and foliose. Lichen samples were grown on the bark of 9 trees species in CSC and CBG. Isolation process used three agar media; HV, YIM6 and YIM711 with cycloheximide and nalidixic acid. Molecular identification based on 16S rRNA gene sequence. Antimicrobial activity was tested to 65 isolates by agar diffusion method to Bacillus subtilis BTCC B.612, Escherichia coli BTCC B.614, Candida albicans BTCC Y.33, Staphylococcus aureus BTCC B.611, Micrococcus luteus BTCC B.552. Isolation process retrieved 125 isolates with the highest number grow on HV agar medium. Based on the sample, the highest number of actinomycetes were isolated from crustose lichen attached on the bark of Averrhoea carambola. A total 69 isolates were identified as the genera Actinoplanes, Amycolatopsis, Angustibacter, Kribbella, Micromonospora, Mycobacterium, and Streptomyces. The screening process showed 24 isolates have antimicrobial activity, with the highest inhibitory activity against Micrococcus luteus BTCC B.552.
OPTIMASI DAN PEMEKATAN LIPASE Bacillus halodurans CM1 Aisyah, Arina; Mangunwardoyo, Wibowo; Trismilah, Trismilah; Suhendar, Dadang
Al-Kauniyah: Jurnal Biologi Vol 10, No 2 (2017): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (570.189 KB) | DOI: 10.15408/kauniyah.v10i2.4908

Abstract

Abstrak Lipase diketahui memiliki peranan penting dalam bidang industri. Produksi lipase dapat dihasilkan oleh kapang, khamir, dan bakteri. Penelitian bertujuan untuk meningkatkan aktivitas lipase yang dihasilkan oleh Bacillus halodurans CM1. Aktivitas lipase dapat ditingkatkan dengan optimasi komposisi media, mutasi bakteri dengan radiasi gamma dan N-methyl-N’-nitro-N-nitrosoguanidine (NTG). Enzim yang dihasilkan dipekatkan dengan metode stirred-cell ultrafiltration (UF)-ammonium sulfat dan UF-Polyethylene glycol (PEG). Uji aktivitas dilakukan pada tujuh media yang berbeda untuk mendapatkan media produksi. Delapan variabel komposisi media dioptimasi dengan rancangan Plackett-Burman. Bakteri dimutasi dengan radiasi gamma dosis 0,1–0,4 kGy dan NTG 0,05–0,15 mg/mL dengan waktu inkubasi 1–3 jam. Hasil penelitian menunjukkan bahwa media produksi yang digunakan berdasarkan optimasi media dan komposisi media Plackett-Burman adalah media dasar Bora & Bora yang mengandung 0,5% palm oil (PO) dan 0,09% CaCl2. Aktivitas lipase optimal diproduksi oleh bakteri hasil mutasi dengan NTG 0,1 mg/mL yang diinkubasi selama 3 jam. Pemekatan enzim UF-ammonium sulfat dan UF-PEG mampu meningkatkan aktivitas enzim lipase sebesar 18,44%.  Abstract Lipase is known to have an important role in the industrial field. Lipase can be produced by molds, yeasts, and bacteria. The research aimed to increase the activity of lipase produced by Bacillus halodurans CM1. Lipase activity can be improved by optimization of the composition of the media, the mutation of bacteria with gamma radiation and N-methyl-N'-nitro-N-nitrosoguanidine (NTG). The enzyme was concentrated by stirred-cell ultrafiltration method (UF)-ammonium sulfate and UF-Polyethylene glycol (PEG). The activity test was performed on seven different media to get production media. The eight variables of the media composition were optimized by Plackett-Burman design. The bacteria were subject to mutation by using 0.1–0.4 kGy dose of gamma radiation and 0.05–0.15 mg/mL NTG with incubation time for 1–3 hours. The results showed that the production media used based on optimization and composition of Plackett-Burman media was Bora Bora medium that containing 0.5% palm oil (PO) and 0.09% CaCl2. Optimum lipase activity was produced by the bacterium that mutated with 0.1 mg/mL NTG, incubated for 3 hours. The concentrated by UF-ammonium sulfate and UF-PEG could increase the lipase activity by 18.44%.
VARIASI INTRASPESIES LACTOBACILLUS PLANTARUM (ORLA-JENSEN) BERGEY ET AL. ASAL SAYUR ASIN BERDASARKAN ANALISIS MOLEKULER ., Sulistiani; ., Abinawanto; Sukara, Endang; Dinoto, Achmad; Mangunwardoyo, Wibowo
JURNAL BIOLOGI INDONESIA Vol 11, No 1 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v11i1.2162

Abstract

The current study is the first report on intraspecies analysis of L. plantarum from sayur asin in Indonesia using molecular approach. Three molecular techniques, i.e., restriction fragment length polymorphism (RFLP) 16S-23S rDNA intergenic spacer region (ISR), random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) and enterobacterial repetitive intergenic consensus (ERIC-PCR) were used to determine the intraspecies diversity of L. plantarum responsible for spontaneous fermentation in sayur asin. These methods were aimed to discriminate 46 isolates of L. plantarum isolated from sayur asin, including the type strain. PCR amplification of the 16S-23S rDNA ISR revealed two-bands profile of 800 and 600 bp specific to lactobacilli. RAPD-PCR and ERIC-PCR were very valuable in discriminating genetic polymorphism among L. plantarum isolates by producing bands ranged from 4-10 bands (360-2620 bp) and 6-12 bands (160-2900 bp), respectively. Dendograms generated from UPGMA cluster analysis based on RAPD-PCR and ERIC-PCR data showed that all isolates were grouped into three major clusters with 74% and 68.6% genetic similarity thresholds, respectively.The study indicated that strains belong to L. plantarum isolated from sayur asin were divided into three genotypic groups. Keywords: ERIC-PCR, Intraspecies, Lactobacillus plantarum, RAPD-PCR, RFLP 16S-23S rDNA ISR 
PENINGKATAN AKTIVITAS LIPASE KAPANG LIMBAH KERNEL DAN NUT KELAPA SAWIT DENGAN RADIASI GAMA DAN ULTRAVIOLET Indriawan, Aris; Mangunwardoyo, Wibowo; Suhendar, Dadang; Siswodarsono, Trismilah
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 5 No. 2 (2018): December 2018
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (516.758 KB) | DOI: 10.29122/jbbi.v5i2.2991

Abstract

Enhancement of Lipase Activity of Molds Isolated from Kernel and Nut Waste of Oil Palm with Gamma and Ultraviolet IrradiationABSTRACTMolds isolated from oil palm waste sampled from Malingping, Lebak, Banten, West Java have the potential for lipase production. This study aimed to increase the fungal lipase activity with gamma radiation and ultraviolet light (UV). NA and KC mold spores were exposed to various gamma radiation doses of 1, 2, 3 and 4 kGy. The best of these NA and KC resulted mutants were followed by ultraviolet mutations for 1, 2, 3, and 4 hours, at dose of 0.1 J/cm2, 254 nm, 20 cm. Lipase activity was tested by the Lindfield method. The results showed that gamma radiation affected the lipase activity of NA1kGy mutants (8.58 U/mL) and KC1 kGy (8.25 U/mL), each increased the lipase activity by 4.6% and 3.13% from the wild type, respectively. Mutations with ultraviolet had an effect on mutant lipase activity of KC4H 10U/mL and NA3H 9.25 U/mL, each increased the lipase activity by 25% and 15.63% from the wild type, respectively. Based on phenotypic and phylogenetic (28srRNA) approaches, a mold of KC had a 100% similarity with Aspergillus fumigatus strain RA204.Keywords: gamma radiation, KC mold, lipase, NA mold, ultraviolet light ABSTRAKKapang dari limbah kelapa sawit diisolasi dari Malingping, Lebak, Banten, Jawa Barat berpotensi untuk menghasilkan lipase. Penelitian ini betujuan meningkatkan aktivitas lipase kapang dengan radiasi sinar gama dan sinar ultraviolet (UV). Spora kapang NA dan KC dipaparkan pada berbagai radiasi gama dosis 1, 2, 3 dan 4 kGy. Hasil terbaik dari mutan NA dan KC dilanjutkan dengan mutasi ultraviolet dengan lama inkubasi 1, 2, 3, dan 4 jam, dosis 0,1 J/cm2, 254 nm, 20 cm. Aktivitas lipase diuji dengan metode Lindfield. Hasil penelitian menunjukkan bahwa radiasi gama berpengaruh pada aktivitas lipase mutan NA 1kGy 8,58 U/mL dan KC1 kGy 8,25 U/mL, masing-masing menaikkan aktivitas lipase sebesar 4,6% dan 3,13% dari wild type-nya. Hasil mutasi dengan ultraviolet berpengaruh pada aktivitas lipase mutan KC4H 10U/mL dan NA3H 9,25 U/mL, masing-masing menaikkan aktivitas lipase sebesar 25% dan 15,63% dari wild type-nya. Berdasarkan pendekatan fenotipik dan filogenetik (28s rRNA), isolat kapang kernel C memiliki similiaritas 100% dengan spesies Aspergillus fumigatus strain RA204.Kata Kunci: kapang KC, kapang NA, lipase, radiasi sinar gama, sinar ultraviolet
Sugarcane Bagasse as a Carrier for the Immobilization of Saccharomyces cerevisiaein Bioethanol Production Anita, Sita Heris; Mangunwardoyo, Wibowo; Yopi, Yopi
Makara Journal of Technology Vol. 20, No. 2
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Sugarcane bagasse was used as a carrier to immobilize Saccharomyces cerevisiae in bioethanol production. This research aims to study the potential use of sugarcane bagasse as an alternative carrier for cell immobilization and improvement in the production process of cell immobilization in bagasse. The results showed that the physical characteristics of sugarcane bagasse as a carrier were water content (7.77 ± 0.35%), water retention (4.80 ± 0.44 g/g), water absorption index (8.58 ± 0.22 g/g), and lignin content (24.40 ± 1.52 %). Determination of cell retention was performed in an inoculum volume of 50 mL yeast suspension with various carrier weights (2.5, 5, 10, and 20 g). The highest cell retention was obtained in ratio of 2.5 g carrier/50 mL cell suspension with cell retention of 5.41 ± 1.06 mg/g, or known as biocatalyst. Biocatalyst, as much as 1.5, 3, 4.5, and 6 g, were used as inoculum for a 24 hour bioethanol fermentation. The best concentration and productivity of bioethanol, obtained by using 3 g of biocatalyst, were 23.95 ± 0.28 g/L and 1.24 ± 0.01 g/L/hours. The average of bioethanol yield for a 24 hour fermentation by using immobilized cells was three times higher than the free cells system.
Co-Authors Abinawanto Abinawanto Achmad Dinoto Aisyah, Arina Andi Salamah ARIYANTI OETARI Atit Kanti Bustamam, Alhadi Caniago, Asnany Caterina A, Ines Irene Chandra, Nicholas Dwi Desyandri Desyandri Ekowati Chasanah Endang Sri Heruwati Endang Sukara Fitri, Reno Gintung Patantis Guswenrivo, Ikhsan I Made Sudiana Idris Idris Ifah Munifah Ikhwani, Azra Zahrah Nadirah INDRAWATI GANDJAR Indriawan, Aris Indriawan, Aris Irawan Sugoro Kafi, Rahmat Al Lily Ismaini Lusini, Yuyun Napitupulu, Toga Pangihotan Pratama, Aulia Brellian Pratitis, Asri PUSPITA LISDIYANTI Sari, Arina Findo Setiawan, Fery Hadi SHANTI RATNAKOMALA Siswodarsono, Trismilah Siswodarsono, Trismilah Sita Heris Anita Sophia, Romauli Aya Srikandace, Yoice Suhendar, Dadang Suhendar, Dadang Sukma Nuswantara Sulistiani ., Sulistiani Sulistiani Sulistiani, Sulistiani Susanti, Agustina Eko Syafriana, Vilya Trismilah Trismilah YOPI YOPI Yuliar Yuliar Yusro Nuri Fawzya