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All Journal Journal of Food and Pharmaceutical Science Jurnal Ilmiah Farmasi Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Dentika Dental Journal Pharmaciana: Jurnal Kefarmasian Indonesian Journal of Cancer Chemoprevention The Indonesian Journal of Dental Research Majalah Obat Tradisional INDONESIAN JOURNAL OF PHARMACY Jurnal Farmasi Sains dan Komunitas PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) JFIOnline Media Farmasi Indonesia Jurnal Kefarmasian Indonesia
Sudibyo Martono
Faculty Of Pharmacy, Universitas Gadjah Mada Yogyakarta, Indonesia
Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health Other

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PENGARUH MEDIUM DISSOLUSI DAN PENGGUNAAN SINKER TERHADAP PROFIL DISOLUSI TABLET FLOATING ASPIRIN Agus Siswanto; Achmad Fudholi; Akhmad Kharis Nugroho; Sudibyo Martono
PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) Jurnal Pharmacy, Vol. 11 No. 02 Desember 2014
Publisher : Pharmacy Faculty, Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/pji.v11i2.840

Abstract

ABSTRAK Disolusi merupakan faktor penting dalam pelepasan dan pengembangan sediaan obat. Selain sifat fisika kimia obat, formulasi, dan fabrikasi sediaan; kondisi uji disolusi juga mempengaruhi profil disolusi termasuk jenis medium dan model alat uji. Tujuan penelitian ini adalah untuk mengetahui pengaruh medium disolusi dan penggunaan sinker terhadap profil disolusi tablet floating aspirin. Tablet dibuat dengan metode cetak langsung dengan bahan tambahan Methocel K4M CR, NaHCO3, Ethocel, Aerosil, dan dikalsium fosfat anhidrat. Tablet diuji disolusi menggunakan alat disolusi USP apparatus 2 dengan pengaduk dayung. Medium disolusi yang digunakan yaitu simulated gastric fluid (SGF) tanpa pepsin pH 1,2 dan HCl 0,1 N. Uji disolusi dengan SGF tanpa pepsin pH 1,2 dilakukan dengan dan tanpa sinker. Suhu percobaan 37 ± 0,5 °C dan kecepatan pengadukan 60 rpm. Cairan sampel diambil pada menit ke-15, 30, 45, 60, 90, 120, 180, 240, 300, 360, 420, dan 480. Serapan sampel diukur dengan spektrofotometer UV pada λ 280 nm. Hasil uji disolusi menunjukkan bahwa perbedaan medium yaitu HCl 0,1 N dan SGF tidak mempengaruhi DE480, kinetika, dan mekanisme disolusi tablet floating aspirin. Penggunaan sinker pada medium SGF mempengaruhi DE480, kinetika, dan mekanisme disolusi. Kurva disolusi tablet floating aspirin pada medium HCl 0,1 N, SGF dengan dan tanpa sinker mengikuti kinetika orde I dan mekanisme disolusi menurut model Korsmeyer-Peppas, Weibull, Hopfenberg, dan Hixson-Crowell. Model Higuchi juga sesuai untuk profil disolusi tablet floating aspirin dalam medium SGF dengan sinker. Kata kunci: tablet floating aspirin, medium disolusi, sinker, profil disolusi ABSTRACK Dissolution is an important factor in the release and development of drug dosage form. In addition to physical and chemical properties of the drug, formulation, manufacturing process; the conditions of dissolution test also affect the profile of dissolution including the type of medium and test equipment models. The purpose of this study was to determine the influence of the dissolution medium and the use of sinker on dissolution profile of aspirin floating tablet. The tablets were made by direct compression method with Methocel K4M CR, NaHCO3, Ethocel, Aerosil, and dicalcium phosphate anhydrous as excipients. The in vitro dissolution study was determined using USP apparatus 2 (paddle method), 900 mL dissolution medium at 37 ± 0.2 °C and 60 rpm. The dissolution test using HCl 0.1 N and simulated gastric fluid (SGF) pH 1.2 as medium with and without a sinker. Aliquouts of 5 mL was taken out at intervals of 15, 30, 45, 60, 120, 180, 240, 300, 360, 420, and 480 minutes. The samples were analyzed by UV-Vis spectrophotometer at 280 nm. The result indicated that the difference of medium (HCl 0.1 N and SGF pH 1.2) does not affect DE480, kinetics, and mechanism of dissolution. The use of sinker in SGF affects DE480, kinetics, and mechanism of dissolution. The profile of dissolution of aspirin floating tablets in 0.1 N HCl and SGF pH 1.2 (with and without sinker) follow first-order kinetics and mechanism of dissolution according to Korsmeyer-Peppas, Weibull, Hopfenberg, and Hixson-Crowell models. Higuchi model was also suitable for dissolution profile of aspirin floating tablet in the SGF pH 1.2 with sinker. Keywords: floating tablet of aspirin, medium of dissolution, sinker, profile of dissolution
Identifikasi Gelatin dari Anjing Berdasarkan Profil Asam Amino dan Kemometrik Wiranti Sri Rahayu; Abdul Rohman; Sudibyo Martono
PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) Jurnal Pharmacy, Vol. 14 No. 02 Desember 2017
Publisher : Pharmacy Faculty, Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (749.131 KB) | DOI: 10.30595/pharmacy.v14i2.2021

Abstract

Gelatin adalah protein yang diperoleh dengan memanaskan kulit, tendon, ligamen, dan tulang dengan air. Gelatin banyak digunakan di beberapa produk makanan dan industri farmasi. Beberapa agama seperti Islam melarang pengikutnya mengkonsumsi produk makanan yang mengandung keturunan babi dan anjing, termasuk gelatin. Deteksi adanya kandungan nonhalal dalam produk pangan telah menjadi studi penting di banyak negara. Penelitian ini bertujuan untuk membedakan gelatin anjing dari kambing, sapi dan babi berdasarkan profil asam amino yang dikombinasikan dengan kemometrik dari komponen utama analisis (Principal Component Analysis/PCA). Pemisahan dan penentuan asam amino menggunakan liquid chromatography mass spectroscopy. Hasil penelitian menunjukkan bahwa lima kandungan asam amino tertinggi dalam gelatin anjing adalah asam glutamat, glisin, alanin, arginin, dan metionin. Parameter persentase tinggi puncak masing-masing asam amino dari masing-masing sampel dianalisis dengan PCA. Berdasarkan PC1 dan PC2, gelatin dari anjing, kambing, sapi, dan babi bisa dibedakan. 
ANALISIS DAIDZEIN DAN GENISTEIN PADA KEDELAI (Glycine max L. Merril) VARIETAS ANJASMORO, ARGOMULYO DAN DENA 2 MENGGUNAKAN METODE KCKT Sulistyowati, Etty; Martono, Sudibyo; Riyanto, Sugeng; Lukitaningsih, Endang
Media Farmasi Indonesia Vol. 13 No. 1 (2018): Media Farmasi Indonesia
Publisher : SEKOLAH TINGGI ILMU FARMASI YAYASAN PHARMASI SEMARANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (508.583 KB)

Abstract

ABSTRAK Kedelai (Glycine max L. Merril) tidak hanya digunakan sebagai sumber protein, tetapi juga sebagai pangan fungsional yang dapat mencegah timbulnya penyakit-penyakit degeneratif, dikarenakan kandungan isoflavon. Senyawa ini dikenal memiliki kesamaan molekul dengan estrogen. Penggunaan isoflavon sebagai alternatif hormon konvesional terapi telah meningkat dalam beberapa tahun terakhir, karena aktivitas estrogenik dan efek samping yang rendah. Daidzein dan genistein merupakan isoflavon yang banyak terdapat dalam kedelai. Penelitian ini bertujuan untuk mengetahui kandungan daidzein dan genistein pada kedelai (Glycine max L. Merril) varietas Anjasmoro, Argomulyo dan Gema yang dihasilkan dari Balai Penelitian Tanaman Aneka Kacang dan Umbi (Balitkabi) Malang. Penyarian senyawa aktif digunakan metanol hasil optimasi, selanjutnya dianalisis kandungan daidzein dan genistein dengan Kromatografi Cair Kinerja Tinggi (KCKT). Sistem KCKT yang digunakan dilengkapi kolom RP-C18 Sun Fire TMC-18 (150 mm x 4,6 mm,5µm), detektor Photo Dioda Array (PDA), sistem elusi isokratik, fase gerak metanol-air yang mengandung asam asetat 0,1 % (53:47), kecepatan alir 1,0 mL/menit. Hasil penelitian menunjukkan bahwa kandungan daidzein dan genistein pada kedelai (Glycine max L. Merril) varietas Anjasmoro, Argomulyo dan Gema diperoleh kadar daidzein dan genistein masing-masing adalah 18,69 mg/100g dan 23,67 mg/100g; 29,68 mg/100 g dan 22,15 mg/100 g; 14,15 mg/100 g dan 21,22 mg/100 g.
Validasi Metode HPLC untuk Penetapan Aspirin dan Asam Salisilat dalam Plasma Kelinci (Lepus curpaeums) secara Simultan Siswanto, Agus; Fudholi, Achmad; Nugroho, Akhmad Kharis; Martono, Sudibyo
Jurnal Kefarmasian Indonesia VOLUME 6, NOMOR 2, AGUSTUS 2016
Publisher : Pusat Penelitian dan Pengembangan Biomedis dan Teknologi Dasar Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jki.v6i2.2922

Abstract

Aspirin is a nonsteroidal anti-inflammatory drug which also has the effect of antiplatelet for stroke prevention. Aspirin inside human body is very easy to break down into salicylic acid as the main metabolite. The aim of this study is to develop and validate the method for determinating aspirin and salicylic acid concentration in plasma by HPLC. Method validation including system suitability test, linearity test, determination of LOD and LOQ, recovery, accuracy and precision. Concentration of analytes in blood is measured by HPLC using benzoic acid as internal standard, with condition Purospher column Endcapped Star RP-18 (250 x 4.6 mm id, 5 m), acetonitrile : buffer phosphate 20 mM pH 2.5 (30:70 v/v) as mobile phase, injection volume 20 mL, flow rate 1.5 mL/minute, and UVVis detector λ 230 nm. The results showed that the proposed method meets the requirements of system suitability and good linearity (r > 0,990) with LOQ (aspirin = 0.024 mg/mL, salicylic acid = 0.336 mg/mL) and LOD (aspirin = 0.007 mg/mL, salicylic acid = 0.101 mg/mL). The method of analysis provides recovery of 85-115 %, accuracy and precision in accordance with the requirements for bioanalytical with CV < 5 %. Therefore, the proposed method is applicable to determine of aspirin and salicylic acid concentration in plasma.
Uji Bioavailabilitas Tablet Floating Aspirin Siswanto, Agus; Fudholi, Achmad; Nugroho, Akhmad Kharis; Martono, Sudibyo
Jurnal Kefarmasian Indonesia VOLUME 7, NOMOR 2, AGUSTUS 2017
Publisher : Pusat Penelitian dan Pengembangan Biomedis dan Teknologi Dasar Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jki.v7i2.3498

Abstract

Aspirin is a non-steroidal anti-inflammatory drug with potential as antiplatelet for stroke prophylaxis. Several approaches of aspirin formulations in various dosage forms have been performed. Formulations of aspirin in conventional tablet dosage form often cause gastric irritation. Aspirin is rapidly absorbed in the upper gastrointestinal tract, especially the first small intestine. Therefore formulation of floating drug delivery system are designed to improve the bioavailability of aspirin. The floating system will retain the tablet in stomach, allowing sufficient absorption time for aspirin in stomach and upper intestine. Aim of this study was to determine relative bioavailability of aspirin floating tablets compared to the aspirin enteric coated tablets in rabbits with crossover design method. Serial blood samples were collected from rabbit ear marginal vein over a 10-h period. Drugs concentration in plasma (aspirin and salisylic acid) were determined by HPLC with benzoic acid as internal standard. The results showed that the floating aspirin tablet has better bioavailability with shorter tmax and more uniform of aspirin levels compared to enteric coated tablets, though the parameters AUC and Cpmax both of those products were not significant (p> 0.05).
Activity of Noni Fruit (Morinda citrifolia L.) Ethanolic Extract on Class mu Glutation S-Transferase of Lung Rat Purwanto, Purwanto; Martono, Sudibyo
Indonesian Journal of Cancer Chemoprevention Vol 11, No 1 (2020)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev11iss1pp46-53

Abstract

One of the main modalities of cancer treatment is chemotherapy, which uses chemicals that are generally electrophilic. These xenobiotic compounds sometimes does not produce effective response due to activity of glutathione S-transferase (GST) which inactivate the xenobiotics. Several natural phenolic compounds were reported to inhibit GST activity in vitro. Noni fruit (Morinda citrifolia L.) which contains flavonoids and other phenolic compounds such as scopoletin and morindon is proposed to interfere GST activity. This study aimed to analyze the effect of ethanolic extract of Noni fruit in vivo on GST activity in lung rat using 1,2-dichloro-4-nitrobenzene (DCNB). This substrate is a specific for class mu GST. First, rats were administered with ethanolic extract of Noni and dimethylbenz(α)anthracene (DMBA) for two weeks. The cytosolic fraction of lung was isolated then the GST activity was determined by simple kinetic program which was automatically calculated using spectrophotometer. The results showed that ethanolic extract of Noni in 1 and 5% (w/v) of concentration induced class mu GST activity, whereas 10% (w/v) of concentration inhibited class mu GST activity. After a treatment with DMBA, all tested concentrations of ethanolic extract of Noni inhibited class mu GST activity of lung rat significantly. These results indicated that Noni fruit extract can be further developed as a supportive agent of a chemotherapy drug.Keywords: DMBA, GST, Morinda citrifolia L., spectrophotometer.
Co-Authors . Sugiyanto A. Margono, Supardjan Abdul Rohman Abdul Rohman Achmad Fudholi Achmad Fudholi Agung Endro Nugroho Agus Siswanto Agus Siswanto Agustinus Yuswanto, Agustinus Akhmad Kharis Nugroho Anak Agung Istri Sri Wiadnyani Any Guntarti Beti Pudyastuti Endang Lukitaningsih Etty Sulistyowati Harsini Harsini ,, Harsini I Dewa Gde Mayun Permana Iwa Sutardja, Iwa Iwa Sutardjo Iwa Sutardjo, Iwa Marchaban Marchaban, Marchaban Marchaban, . Marchaban, . Melania Perwitasari, Melania Mimiek Murrukmihadi Mochammad Imron Awalludin Nina Salamah Nunung Yuniarti Purwanto Purwanto Reyna Nuvitasari Ririn Sumiyani Riyanto, Sugeng Rohman, Abdul SATRIYAS ILYAS Siti Sunarintyas Siti Zahliyatul Munawiroh Subagus Wahyuono Sudarsono Sudarsono Sugeng Riyanto Supardjan A.M., Supardjan Supardjan Amin M Susilowati, Sri Sutji Wiranti Sri Rahayu Yuny Erwanto