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Temperature and holding time of instrument sterilization as an infection control of odontectomy Meliawaty, Florence; Mangundjaja, Sunardhi; Hardjawinata, Karlina
Padjadjaran Journal of Dentistry Vol 23, No 1 (2011): March
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3763.687 KB) | DOI: 10.24198/pjd.vol23no1.14059

Abstract

Odontectomy should be performed aseptically. The goal of sterilization is the complete killing of all forms of microbial life including bacterial spores on the items being processed. Biologic monitoring provides the main guarantee of sterilization. The aim of this study was to find the interrelation of the temperature and the holding time of instrument sterilization as an infection control for the successful of lower molars odontectomy. This experimental laboratory study was conducted at the Oral Maxillofacial Surgery Department in the Hasan Sadikin General Hospital Bandung and at the Microbiology Laboratory Faculty of Dentistry, Universitas Padjadjaran, Jatinangor. The Protocol was performed in three methods of sterilization: dry heat with oven and ozone, dry heat with oven and infrared (125)C for 15 minutes), both were monitored by Bacillus atrophaeus as the biologic indicators, and autoclavization (121" C for 15 minutes) with Geobaciflus stearothermophilus as the biological monitoring, with 17 times repetition. After sterilization, all of the indicators were cultured on Nutrient Agar Plate (NAPS), and the subsequent growth was assessed. The colony forming units (CFUs) were counted by Stuart Electric Bacteria Colony Counter. Adequate positive and negative controls were used in every cycle. The results showed that after autoclavization, all spores were killed. In comparison with dry heat in the oven, there were still CFUs on the NAPs, but no colonies grow after 3 repetitions by oven and infrared. Heating in oven and ozone could only reduce the spore numbers, even after repeating 5 times. The reduction of the CFUs was greater in more repetition. According to the statistical analysis, the differences were significant. This study concluded that sterilization by oven and infrared will be achieved after 3 holding times (30-35 minutes) and dry heat with oven and ozone could only act as a germicide. In autoclavization, all of Geobacillus steorothermophilus have been killed.
STEVENS JOHNSON SYNDROME DISEBABKAN OLEH DRUG ERUPTION OBAT ANALGETIK Putriani, Wivda; Meliawaty, Florence
Medika Kartika : Jurnal Kedokteran dan Kesehatan Vol 1 No 1 (2018): Medika Kartika Edisi PIT 10 Kedokteran Gigi
Publisher : Fakultas Kedokteran Universitas Jenderal Achmad Yani

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Abstract

Stevens Johnson Syndrome adalah satu bentuk reaksi obat pada kulit yang parah, yang ditandai dengan demam dan luka mukokutan yang berlanjut menjadi nekrosis dan epidermis terkelupas.  Laporan kasus ini merupakan tipe observasi di ruangan perawatan rumah sakit, yang disebabkan penggunaan asam mefenamat setelah ekstraksi gigi. Reaksi obat yang parah seperti Stevens Johnson Syndrome dapat berpotensi mengancam jiwa yang memerlukan perawatan ekstra.
PENGARUH PEMBERIAN MADU RAMBUTAN SECARA TOPIKAL TERHADAP PROSES PENYEMBUHAN LUKA SETELAH PENCABUTAN GIGI KELINCI (Oryctolagus cuniculus) Euis Reni Yuslianti; Afifah B Sutjiatmo; Kertamaya Sundawan; Florence Meliawaty
Alami Journal (Alauddin Islamic Medical) Journal Vol 3 No 2 (2019): JULY
Publisher : Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (161.352 KB) | DOI: 10.24252/alami.v3i2.9359

Abstract

Madu rambutan merupakan cairan kental alami yang dihasilkan oleh lebah dari sari bunga tanaman rambutan. Madu rambutan memiliki sifat sebagai antioksidan, antiinflamasi, antibakteri, dan memiliki komposisi kadar kalsium. Penelitian ini bertujuan untuk mengetahui pengaruh madu rambutan yang diberikan secara topikal terhadap proses penyembuhan luka jaringan lunak dan jaringan keras dilihat dari panjang mesial-distal luka dan kadar alkaline phosphatase. Metode penelitian menggunakan rancangan penelitian eksperimental. Obyek penelitian yaitu 30 ekor kelinci yang dibagi tiga kelompok dan dilakukan pencabutan gigi insisif kiri rahang bawah, kelompok kontrol negatif, kelompok kontrol positif yang diberikan povidone iodine, dan kelompok perlakuan yang diberikan madu rambutan. Pengukuran panjang mesial-distal luka menggunakan jangka kaliper dan kadar alkaline phosphatase menggunakan spektrofotometer dengan panjang gelombang 405 nm. Data dianalisis dengan menggunakan analisis statistik Saphiro Wilk, Kruskal-Wallis dilanjutkan dengan Mann Whitney.Hasil penelitian dari ketiga kelompok menunjukkan terdapat perbedaan panjang mesial-distal luka pada hari ke-7 dengan nilai p 0,000 dan ke-14 dengan nilai p 0,033, dan tidak terdapat perbedaan yang signifikan dari uji statistik terhadap enzim alkaline phosphatase.Madu rambutan mengandung enzim katalase, glukosa cukup tinggi, pH rendah, dan hidrogen peroksida sebagai antibakteri sehingga madu rambutan yang diberikan secara topikal dapat membantu penyembuhan luka setelah pencabutan gigi dilihat dari panjang mesial-distal luka, namun tidak berpengaruh terhadap enzim alkaline phosphatase karena sampel yang digunakan merupakan sirkulasi darah sistemik.
Maternal Education Level and Child's Anxiety on Dental Extraction Jeffrey Jeffrey; Florence Meliawaty; Asih Rahaju
Journal of Medicine and Health Vol. 2 No. 1 (2018)
Publisher : Universitas Kristen Maranatha

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (277.089 KB) | DOI: 10.28932/jmh.v2i1.738

Abstract

Dental extraction can cause anxiety in children, observed in vital signs changes. If occursexcessively, it can be regarded as an obstacle and may affect a child's behavior that can furtherdetermine the success of dental treatment. Anxiety that occurs in children can usually be influencedby various factors, including the level of parental education. This study aimed to observe maternaleducation level and anxiety in children receiving dental extraction treatments. This is a descriptivestudy, involving 60 subjects aged 7-12 that went for dental extraction at Rumah Sakit Gigi MulutPendidikan Universitas Jenderal Achmad Yani (RSGMP UNJANI) Cimahi Juli-Desember 2016. Theparameters examined for measuring anxiety are body temperature, respiration frequency, and pulsebefore and after the procedure. This study found that 63.3% of mother of the subject had high schooleducation, 16.7% of junior high school, 8.3% S1, 6.7% D1/D3, and 5% SD. Increased bodytemperature was obtained in 36 subjects, increased respiration frequency in 46 subjects, andincreased pulse in 34 subjects. We concluded that the majority of maternal education levels in thisstudy are high school graduates and the anxiety parameters in the majority of subjects increase whenreceiving dental extraction procedure.Keywords: child's anxiety, dental extraction, mother education level
DIFFERENCES IN SYMPTOMS OF MEN AND WOMEN TEMPOROMANDIBULAR JOINT DISORDERS IN STUDENTS OF MEDICAL FACULTY UNIVERSITAS JENDERAL ACHMAD YANI BATCH 2020 Azkya Patria Nawawi; Florence Meliawaty; Vivin Wijami Putri
Journal of Health and Dental Sciences 2022: Journal of Health and Dental Sciences
Publisher : Fakultas Kedokteran Gigi Unjani

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Abstract

Temporomandibular joint disorders (TMD) become one of the most common muscles and jaw problems in the population aged 20-40. TMD has a higher prevalence in women than men with a ratio between 3-4: 1 because gender is the most related risk factor in TMD. This study aims to determine the symptoms of TMD based on gender in students of the Faculty of Medicine of Universitas Jenderal Achmad Yani, batch 2020. The research used an analytical study using the cross-sectional method. The data were analyzed using a chi-square test with 152 research subjects. The results found that 50.7% of students experienced symptoms of TMD, whereas male students experienced TMD more often (53.2%) than females (49.5%), with a value of p = 0.727. In this study, there was no relationship between the symptoms of temporomandibular joint disorder and gender. DOI : 10.54052/jhds.sd22.p123-132
ACUTE TOXICITY TEST OF RAMBUTAN HONEY ADHESIVE CREAM AGAINST SWISS WEBSTER MICE (UJI TOKSISITAS AKUT KRIM PEREKAT MADU RAMBUTAN TERHADAP MENCIT SWISS WEBSTER) Euis Reni Yuslianti; Florence Meliawaty; Afifah B. Sutjiatmo; Ariana Pradita
Journal of Health and Dental Sciences 2022: Journal of Health and Dental Sciences
Publisher : Fakultas Kedokteran Gigi Unjani

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Abstract

Denture adhesive is indicated for conditions of poor retention and stabilization. Commercially available denture adhesives have side effects, thus requiring a safer alternative adhesive, one of which is rambutan honey adhesive cream. Rambutan honey is safe and does not cause toxic effects on test animals. This study aimed to observe the impact of acute toxicity of the orally administered rambutan honey adhesive cream on mice. The research method is an experimental laboratory method and was carried out using 24 male and female Swiss Webster mice. The subject was divided into four groups, one being the negative control group. Rambutan honey adhesive cream was given in three dose groups, namely 1250, 2500, and 5000mg/kg BW. Observations made were death, body weight, behavioural changes, and relative organ weight in mice for fourteen days. The analysis test was carried out using the one-way ANOVA (p < 0.05). The results showed that rambutan honey adhesive cream did not cause death and toxicity symptoms in mice. There was no significant increase in body weight in male and female mice in the group. In the control group, the macroscopic necropsy analysis did not show any change in color and organ index of the heart, lung, liver, spleen, and kidney. This study concludes that the oral administration of rambutan honey adhesive cream on male and female Swiss webster mice is safe and is classified as practically non-toxic. DOI : 10.54052/jhds.sd22.p133-144
DENTIGEROUS CYST ASSOCIATED WITH IMPACTED ANTERIOR MAXILLARY (KISTA DENTIGEROUS TERKAIT DENGAN IMPAKSI GIGI ANTERIOR RAHANG ATAS) Florence Meliawaty
Journal of Health and Dental Sciences 2022: Journal of Health and Dental Sciences
Publisher : Fakultas Kedokteran Gigi Unjani

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Abstract

Dentigerous cysts arise from the dental follicle of unerupted teeth and are cysts of the epithelial lining of the jaws. These cysts and unerupted teeth are frequently discovered during routine radiography examinations. Dentigerous cysts are usually asymptomatic unless the cyst grows large enough to cause swelling. Most dentigerous cysts are common in association with third molars and maxillary canines. Clinical, radiological, and histopathologic findings are used to make a diagnosis. We report a case of a dentigerous cyst associated with two impacted anterior maxillary teeth. The care of a dentigerous cyst in the upper left canine region with lateral incisor and canine impaction is described in this case report. The patient was treated surgically by enucleation of the cyst under general anaesthesia. The healing was good two weeks after surgery, according to the examination. DOI : 10.54052/jhds.sd22.p145-151
AMBON BANANA PEEL EXTRACT GEL (Musa paradisiaca var. Sapientum (L.) Kunt) ACCELARATES OF WISTAR RATS GINGIVA WOUND HEALING Florence Meliawaty; Rina Putri Noer Fadilah; Putri Mentari
Journal of Health and Dental Sciences Vol. 1 No. 1 (2021): Journal of Health and Dental Sciences
Publisher : Fakultas Kedokteran Gigi Unjani

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Abstract

A wound is a cut through the anatomical structure of tissue due to an incision of a sharp-edged object. Ambon banana peel contains tannins, flavonoids, and saponins which act as an anti- inflammatory antioxidant and antibacterial properties that facilitate the wound healing process. This research attempted to know the effect of ambon banana peel gel extract (Musa paradisiaca var. Sapientum (L.) Kunt) on wound length healing of gingiva mucosa cuts in Wistar rats. The was a pure experimental study with a sample size of 30 Wistar rats divided into three groups; the treatment group was given 10% ambon banana peel gel extract. The positive control group was assigned 10% povidone-iodine, and the negative control group was given aquades. The research was conducted by making an incision on maxillary gingival mucosa 3 mm wide in the horizontal direction with a depth of 0.25 mm using blade number 11. Observations were performed on days 0, 3, 7, and 14 after treatment using a digital calliper. Data were analyzed using the Kruskal Wallis test and then the post hoc test using the Mann Whitney test (p <0.05 significant). The research results showed that the treatment group given 10% ambon banana peel gel extract had a significant difference with a p-value <0.05 compared to the positive and negative control groups on days 3, 7, and 14. The research shows 10%. The concentration of ambon banana peel gel extract affects the accelerating wound healing process in the gingiva mucosa of Wistar rats. DOI : 10.54052/jhds.v1n1.p89-99
Aplikasi gel serbuk membran telur ayam ras 10% (Gallus gallus domesticus) terhadap penyembuhan luka sayat gingiva tikus galur WistarEffect of 10% chicken egg membrane gel powder (Gallus gallus domesticus) on gingival wound healing time in Wistar strain rats Florence Meliawati; Nadya Paramitha; Atia Nurul Sidiqa; Saskia Lenggogeni Nasroen
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 34, No 2 (2022): Agustus 2022
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jkg.v34i2.36010

Abstract

ABSTRAKPendahuluan: Luka merupakan gangguan kontinuitas lapisan epitel kulit atau mukosa. Gingiva merupakan mukosa mulut yang seringkali mengalami luka yang dapat timbul karena trauma pada perawatan penyakit periodontal ataupun ekstraksi gigi. Membran telur ayam mengandung bahan bioaktif karbohidrat kompleks yang dapat berfungsi mempercepat perbaikan jaringan. Tujuan penelitian menganalisis pengaruh aplikasi gel serbuk membran telur ayam 10% terhadap penyembuhan luka sayat gingiva tikus galur Wistar pada hari ke 3,7, dan 14. Metode: Jenis penelitian eksperimental murni dengan jumlah sampel 30 ekor tikus galur Wistar. Luka sayat sepanjang 3 mm arah horizontal kedalaman 0,25 mm dibuat pada gingiva cekat anterior rahang atas. Kelompok uji terdiri dari 3, yaitu apikasi gel membran telur 10% (n=10), aplikasi povidone iodine 10% sebagai kontrol postitif (n=10), dan aplikasi aquades sebagai kontrol negatif (n=10). Pengamatan panjang luka seluruh sampel dilakukan pada hari ke-0, 3, 7, dan 14. Data yang diperoleh kemudian dianalisis dengan uji Kruskal Wallis dan dilanjutkan uji post hoc Mann Whitney dengan p<0,05. Hasil: Seluruh kelompok mengalami pengurangan panjang luka sayat dimulai dari hari ke 3, 7, hingga 14. Kelompok perlakuan aplikasi gel membran telur 10% dapat menyembuhkan luka sayatan lebih cepat dibandingkan kelompok kontrol positif pada hari ke-3 (1,74±0,14)mm, hari ke-7 (0,81±0,16)mm dan hari ke-14 (0,07±0,14)mm. Pengukuran panjang luka sayat di hari ke-3 dan ke-7 kelompok aplikasi gel membran telur 10% signifikan berbeda dibandingkan kontrol positif (p<0,05). Simpulan: Gel serbuk membran telur 10% dapat mempercepat penyembuhan luka sayat gingiva tikus galur Wistar pada hari ke-3, ke-7 dan ke-14.Kata kunci: luka sayat; membran telur ayam; penyembuhan luka; galur wistar ABSTRACTIntroduction: The epithelial layer of the skin or mucosa is disrupted when there is a wound. The gingiva is the oral mucosa that frequently sustains wounds from trauma that can happen during the treatment of periodontal disease or tooth extraction. Complex carbohydrate bioactive ingredients found in chicken egg membranes, it can speed up tissue repair. This study aimed to analyzed the impact of application egg membrane gel 10% on the length of the wound on Wistar strain on day 3, 7, and 14. Methods: A (3x0.25)mm incision was made on the maxillary anterior attached gingiva Wistar strain rats. There were 3 groups, egg membrane gel 10%, povidone iodine 10% as a positive control, and aquades as a negative control. Observation of wound length for all samples was carried out on days 0, 3, 7, and 14. The data obtained were then analyzed by the Kruskal Wallis test and followed by the Mann Whitney post hoc test with p<0.05. Results: All groups experienced a reduction in the length of the incision starting from days 3, 7, to 14. The egg membrane gel 10% application treatment group healed the incision faster than the positive control group on day 3 (1.74±0.14)mm, day 7 (0.81±0.16)mm, and day 14 (0.07±0.14)mm. The measurement of the length of the incision on days 3 and 7 of the egg membrane gel 10% application group was significantly different from the positive control (p<0.05). Conclusion: egg membrane gel 10% can accelerate the healing of gingival incisions in Wistar strain rats started on days 3 to 14.Keywords: cut wounds; wound healing; chicken egg membrane; wistar strain
UJI DAYA ANTIBAKTERI EKSTRAK DAUN SIRIH MERAH (Piper crocatum) TERHADAP BAKTERI Porphyromonas gingivalis SEBAGAI TERAPI PENUNJANG PERIODONTITIS KRONIS Frita Ferlita Shafri Djohan; Florence Meliawaty
Medika Kartika : Jurnal Kedokteran dan Kesehatan Vol 5 No 3 (2022): Medika Kartika : Jurnal Kedokteran dan Kesehatan
Publisher : Fakultas Kedokteran Universitas Jenderal Achmad Yani

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Abstract

Porphyromonas gingivalis merupakan bakteri patogen dalam penyakit periodontitis kronis. Kandungan senyawa organik daun sirih merah (Piper crocatum) memiliki efek antibakteri. Ekstrak Piper crocatum diharapkan dapat digunakan sebagai terapi penunjang penyakit periodontitis kronis. Penelitian ini bertujuan untuk mengetahui efek antibakteri ekstrak Piper crocatum melalui uji daya hambat terhadap Porphyromonas gingivalis. Prosedur kerja dengan membuat sampel ekstrak etanol 80% daun sirih merah dengan konsentrasi 2,5%; 5%; dan 10% dalam 10 mL DMSO 10%. Kultur biakan bakteri P. gingivalis ATCC 33277 di media Lempeng Agar Darah (LAD). Uji daya hambat dengan metode Kirby Bower pada konsentrasi ekstrak 2.5%, 5%, 10% pengulangan sebanyak tiga kali pada LAD berisi bakteri. Hasil uji daya hambat yaitu tidak didapat zona bening pada konsentrasi ekstrak 2.5%, 5%, 10%. Kontrol positif tidak ada dan kemungkinan bakteri terkontaminasi dapat menjadi penyebab zona bening tidak terbentuk. Ekstrak Piper crocatum pada konsentrasi 2,5%, 5%, dan 10% tidak memiliki efek terhadap bakteri P. gingivalis. DOI : 10.35990/mk.v5n3.p308-320