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Validated TLC-Contact Bioautography Method for Identification of Kanamycin Sulfate in Injection Preparation Susanti Susanti; Aprelita Nurelli Dwiana; Febri Annuryanti; Asri Darmawati; Isnaeni Isnaeni
JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Vol. 7 No. 1 (2020): JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jfiki.v7i12020.35-41

Abstract

Background: TLC-contact bioautography is one of an effective method for identification antibiotics, by which many antibiotics could be identification and determination simultaneously. Objective: To evaluate kanamycin sulfate in injection preparations based on its inhibitory activity against Escherichia coli ATCC 8739 as test organism. Methods: Sample and standard solutions were spotted onto TLC silica gel 60 F254 plate and developed in 10% potassium dihydrogen phosphate solutionas as mobile phase. The TLC-contact bioautography method was  validated according to USP guidelines by considering specificity, LOD, LOQ, linearity, accuracy and precision parameters. Results: The TLC-contact bioautography method was found to be high sensitivity with LOD of 0.75 µg and LOQ 2.31 µg. Linearity range of 100-350 µg/mL with r = 0.9993 and linear regression equation was y = 0.0019x + 0.0338. The recovery obtained from addition of blank samples by three different concentrations of kanamycin sulfate standard was 101.40% ¬+ 2.02%. The precision of the method was good with coefficient of variation 0.080%. The TLC-contact bioautography method was supported by determination of kanamycin sulfate potency ratio in the injection preparation and kanamycin sulfate standard using 3-3 design. Random block design obtained the potential for kanamycin sulfate in injection preparations compared to kanamycin sulfate standard was 100.6%.  Conclusion: The TLC-contact bioautography for kanamycin sulfate in injection preparations could be applied to the quality control analysis of the investigated drugs.
KLT-Bioautografi Ekstrak Etil Asetat Supernatan Hasil Fermentasi Streptomyces G Isolat Tanah Rumah Kompos Bratang Surabaya Ifah Yulistyani; Achmad Toto Poernomo; Isnaeni Isnaeni
JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Vol. 8 No. 1 (2021): JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jfiki.v8i12021.1-9

Abstract

Pendahuluan: Meningkatnya penggunaan antibiotika yang tidak rasional menyebabkan berkembangnya masalah resistensi obat anti infeksi. Perkembangan perilaku mikroorganisme yang luar biasa pesatnya melalui berbagai mekanisme telah melahirkan berbagai strain yang resisten, toleran dan persisten, antara lain, Multi Drug Resistant (MDR), Extended Strain Betalactamase, MDR-Tuberkulosis dan Methicillin Resistant Staphylococcus aureus. Upaya untuk mengeksplor antibiotika baru dari berbagai sumber alam telah banyak dilakukan untuk mengatasi permasalahn terkait kebutuhan antibiotika yang handal dalam mengatasi penyakit infeksi. Tujuan: Penelitian ini berfokus pada isolasi Streptomyces sp.yang mampu menghasilkan senyawa anti bakteri dari tanah kompos buangan sampah di daerah Bratang Surabaya. Metode: Streptomyces sp. telah berhasil diisolasi dan diidentifikasi sebagai Streptomyces G dan dilakukan proses dalam media ISP-4. Metode KLT-bioautografi digunakan untuk mengevaluasi aktivitas antibakteri ekstrak etil asetat supernatan kaldu fermentasi terhadap Escherichia coli ATCC 7890 dan Staphylococcus aureus ATCC 23456. Hasil: Supernatan menunjukkan kemampuan untuk menghambat pertumbuhan bakteri uji. Senyawa aktif berhasil diekstraksi dari supernatan dengan etil asetat dan KLT-bioautogram menggunakan eluen butanol-asam asetat-air (3:2:6, v/v) menunjukkan bahwa ada dua noda yang terpisah secara baik, salah satu dari noda dengan Rf 0,56 mampu menghambat bakteri uji dengan kategori potensi lemah. Kesimpulan: Ekstrak etil asetat supernatan kaldu fermentasi Streptomyces G dalam media ISP-4 mengandung dua senyawa yang berbeda dan satu diantaranya menunjukkan aktivitas penghambatan terhadap pertumbuhan Staphylococcus aureus ATCC 6538 dan Escherichia coli ATCC 8739 berdasarkan data KLT- bioautogram.
Optimasi Kondisi Fermentasi pada Produksi Metabolit Antibakteri dari Bacillus tequilensis BSMF Simbiotik Halichondria panicea Nindya Pramesti Wardani; Achmad Toto Poernomo; Isnaeni Isnaeni
JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Vol. 8 No. 2 (2021): JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jfiki.v8i22021.187-193

Abstract

Pendahuluan: Resistensi antibakteri merupakan masalah kesehatan global yang dialami hampir di seluruh negara. Eksplorasi antibakteri dari sumber baru seperti tumbuhan, hewan, dan mikroorganisme baik yang hidup bebas maupun bersimbiosis menjadi solusi alternatif untuk mengatasi resistensi antibakteri. Mikroorganisme berupa bakteri ditargetkan sebagai sumber antibakteri yang berkelanjutan karena jumlahnya melimpah dan mudah dalam proses pembiakan. Bakteri yang hidup bersimbiosis diketahui dapat memproduksi metabolit antibakteri berspektrum lebih luas dibandingkan bakteri yang hidup bebas. Bakteri dapat bersimbiosis dengan berbagai makhluk hidup termasuk organisme multiseluler seperti spons. Isolat Bacillus tequilensis BSMF yang bersimbiosis dengan Halichondria panicea dari Perairan Cabbiya Madura menunjukkan adanya produksi metabolit yang memiliki aktivitas antibakteri. Tujuan: Menentukan pH dan suhu optimum untuk produksi metabolit antibakteri dari Bacillus tequilensis BSMF simbiotik Halichondria panicea. Metode: Produksi metabolit antibakteri dilakukan dengan metode fermentasi padat pada media Potato Dextrose Agar (PDA) yang telah diatur pH dan suhu inkubasinya, sedangkan uji aktivitas antibakteri terhadap Staphylococcus aureus ATCC 25923 dan Eschericia coli ATCC 25922 dilakukan menggunakan metode difusi agar. Penentuan aktivitas antibakteri dilakukan melalui pengukuran diameter zona hambat. Hasil: pH media yang menunjukkan aktivitas antibakteri optimum Bacillus tequilensis BSMF terhadap Staphylococcus aureus ATCC 25923 dan Eschericia coli ATCC 25922 adalah 8 ± 0,5 pada suhu inkubasi 32 ± 1oC dengan rata- rata indeks aktivitas antibakteri berturut- turut 2,74 ± 0,07 dan 3,39 ± 0,07. Kesimpulan: pH dan suhu optimum yang diperoleh adalah pH 8 ± 0,5 dan suhu 32 ± 1oC.
REVITALISASI USAHA KECIL MENENGAH DHARMA BOGA BOJONEGORO MELALUI PELATIHAN CARA PRODUKSI DAN REGISTRASI PANGAN YANG BAIK SERTA HALAL Isnaeni; Noor Erma Nasution; Sugijanto; Hadi Poerwono; Juni Ekowati; Siswandono; Marcellino Rudyanto; Kholis Amalia Nofianti; Achmad Syahrani; Suzana
Panrita Abdi - Jurnal Pengabdian pada Masyarakat Vol. 5 No. 2 (2021): Jurnal Panrita Abdi - April 2021
Publisher : LP2M Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/pa.v5i2.5754

Abstract

Abstract. The independence of food supply in Indonesia is supported by the abundant diversity of natural ingredients from vegetable, animal and mineral sources. Compared to drugs, dependence on food products in other countries is relatively lower, because the fabrication requires facilities and infrastructure that can be fulfilled domestically. The regulatory system managed by the Republic of Indonesia Drug and Food Control Agency (BPOM-RI) has been implemented to ensure that food products consumed by the public are of high quality, nutritious, safe, halal, and affordable and with a taste that satisfies user communities. Circular-worthy products must at least meet the registration requirements of halal aspects, maximum microbial limits and chemical contamination. The growth of Micro, Small and Medium Enterprises (MSMEs) is also facilitated by the government. Dharma Boga is one of the Small and Medium Enterprises (UKM) in Klangon sub-district, Bojonegoro which is engaged in food production for more than 10 years. Some problems related to registration, halal certification and handling of production, especially food additives that meet requirements, has been communicated to the Community Service Team (PENMAS) Faculty of Pharmacy, Airlangga University (FFUA). Alhamdulillah, PENMAS activities with training materials on how to produce and register good and halal food have been carried out with satisfactory results, although it still needs to be followed up with a partnership for the MSME revitalization program in the future. Of the three parameters used to measure participants' understanding of training material, the average obtained more than 30% showed an increase. For the introduction of the halal logo and the dangers of formaldehyde, 100% of the message is understood. The training involved experts and stakeholders in the authority of the East Java Indonesian Ulama Council and the teaching staff and the Airlangga University Faculty of Pharmacy Testing Service Unit.         Abstrak. Kemandirian penyediaan pangan di Indonesia didukung oleh diversitas bahan alam yang melimpah baik dari sumber nabati, hewani, maupun mineral. Dibandingkan obat, ketergantungan produk pangan pada negara lain relatif lebih rendah, karena fabrikasinya membutuhkan fasilitas dan infrastruktur yang dapat terpenuhi di dalam negeri. Sistem regulasi yang dikelola oleh Badan Pengawas Obat dan Makanan Republik Indonesia (BPOM-RI) telah diberlakukan untuk menjamin produk makanan yang dikonsumsi masyarakat berkualitas, bergizi, aman, halal, dan terjangkau serta dengan rasa yang memuaskan selera masyarakat. Produk yang laik edar minimal harus memenuhi persyaratan registrasi dari aspek halal, batas maksimum mikroba dan cemaran bahan kimia. Tumbuh kembangnya Usaha Mikro, Kecil dan Menengah (UMKM) juga difasilitasi oleh pemerintah. Dharma Boga adalah salah satu Usaha Kecil Menengah (UKM) di kecamatan Klangon, Bojonegoro yang bergerak dibidang produksi makanan selama lebih dari 10 tahun. Beberapa permasalahan terkait registrasi, sertifikasi halal dan penanganan produksi terutama bahan tambahan makanan yang memenuhi persyaratan, telah dikomunikasikan kepada Tim Pengabdian kepada Masyarakat (PENMAS) Fakultas Farmasi Univeritas Airlangga (FFUA). Alhamdulillah kegiatan PENMAS dengan materi pelatihan cara produksi dan registrasi pangan yang baik serta halal telah dilaksanakan dengan hasil yang memuaskan, walaupun masih perlu ditindaklanjuti dengan jalinan kerjasama untuk program revitalisasi UMKM di masa mendatang. Dari tiga parameter yang digunakan untuk mengukur pemahaman peserta terhadap materi pelatihan, rerata yang diperoleh lebih dari 30% menunjukkan peningkatan. Untuk pengenalan logo halal dan bahaya formalin, 100% peserta sudah paham. Pelatihan melibatkan pakar dan pemangku kewenangan dari Majelis Ulama Indonesia Jawa Timur dan para staf pengajar serta Unit Layanan Pengujian Fakultas Farmasi Universitas Airlangga.
Training and Mentoring of Probiotic Milk Fermentation Technology Development in Purwodadi Village, Purwodadi Sub-District, Pasuruan Achmad Toto Poernomo; Asri Darmawati; Isnaeni Isnaeni; Sudjarwo Sudjarwo
Warta Pengabdian Vol 15 No 2 (2021): Warta Pengabdian
Publisher : LP2M Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/wrtp.v15i2.20720

Abstract

Purwodadi sub-district has a surplus of dairy milk with a production of 25 tons of milk per day per KUD. There are 4 milk industry in Purwodadi sub-district as suppliers of PT Nestle. The problem is, dairy products are not entirely absorbed by the market, so it is necessary to look for breakthroughs in the distribution of dairy products that have an average price of IDR 8,000-13,000 per liter. Diversification of milk into fermented milk products will increase prices by five times, so that it is expected to increase people's income, because not only is the product variant increasing, but the market is also increasing. The result of the training shows that all participants experience a significant increase in knowledge, indicated by higher post-test result compared to the pre-test result. Another result known is that this probiotic-fermented milk can be used as a medium for a source of economic improvement for the family. This is proven when yogurt made by the community of Purwodadi has been sold among them as an additional drink during community meetings. Apart from that, this training will create a climate that enables the potential of the Youth Organization (Karang Taruna) and Family Welfare Program (PKK) to develop, namely to build group creative power by encouraging, motivating, and raising awareness of the potential of the village and efforts to develop it.
PENINGKATAN PEMBERDAYAAN KELOMPOK WANITA TANI DUSUN WONOREJO KOTA BATU MELALUI PELATIHAN PEMBUATAN PRODUK SUSU PROBIOTIK-SARI BUAH SEBAGAI MINUMAN KESEHATAN Nuzul Wahyuning Diyah; Isnaeni Isnaeni; Asri Darmawati; Suko Hardjono
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 4 No. 1 (2020): JURNAL LAYANAN MASYARAKAT
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jlm.v4i1.2020.128-133

Abstract

Wonorejo village in Batu city is known as a vegetable and fruit producing area. Another popular product of Batu mostly is fresh dairy milk and only a small amount of processed products while the cow milk collected in KUD Batu has reached 16-17 tons per day. The problem was that the target community had no experiences in processing cow milk using probiotic microbes. The purpose was transfering of science and technology about the production of probiotic milk combined with fruit juice. This program was specifically directed to increase the empowerment of Kelompok Wanita Tani "Wonoasri" in Wonorejo, in developing of fruity- probiotic dairy products to increase the diversification of products with higher economic value. The method included workshop and technical assistance, which were carried out in a serie of steps: 1) exploring the knowledge of target by surveys and pre test, 2) lecturing and discussion, 3) demonstrating how to prepare and characterize the probiotic milk products, 4) training the preparation of product, 5) product characterization and packaging assistance, 6) evaluation. The results of this program were the target community gained increase in knowledge and experience, and they could produce fruity probiotic milk. In addition, we expected that they would increase their understanding and enthusiasm to appreciate the potency of their territory in order to improve the nation's competitiveness. abstrakDesa Wonorejo di kota Batu dikenal sebagai daerah penghasil sayur dan buah. Produk populer lain dari Batu adalah susu segar dan sejumlah kecil produk olahan.  Susu sapi dikumpulkan di KUD Batu. Masalahnya adalah bahwa komunitas target tidak memiliki pengalaman dalam memproses susu sapi menggunakan mikroba probiotik. Tujuannya adalah mentransfer ilmu pengetahuan dan teknologi tentang produksi susu probiotik yang dikombinasikan dengan jus buah. Program ini diarahkan untuk pemberdayaan Kelompok Tani Wanita "Wonoasri" di Wonorejo, dalam mengembangkan produk susu buah-probiotik untuk diversifikasi produk dengan nilai ekonomi lebih tinggi. Metode tersebut termasuk lokakarya dan bantuan teknis, dalam serangkaian langkah: 1) mengeksplorasi pengetahuan target dengan survei dan pra-tes, 2) ceramah dan diskusi, 3) menunjukkan bagaimana mempersiapkan dan mengkarakterisasi produk susu probiotik, 4) pelatihan persiapan produk, 5) karakterisasi produk dan bantuan pengemasan, 6) evaluasi. Hasil program ini adalah komunitas sasaran memperoleh peningkatan dalam pengetahuan dan pengalaman, dan mereka dapat menghasilkan susu probiotik buah. Selain itu, kami berharap bahwa mereka akan meningkatkan pemahaman dan antusiasme mereka untuk menghargai potensi wilayah mereka untuk meningkatkan daya saing bangsa.
Validasi Metode Spektrofotometri UV Untuk Penetapan Kadar Kolkisin Dalam Infus Kembang Sungsang Muhammad Muslich; Isnaeni Isnaeni; Sudjarwo Sudjarwo
Berkala Ilmiah Kimia Farmasi Vol. 7 No. 1 (2020): JUNI
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (306.588 KB) | DOI: 10.20473/bikfar.v7i1.29058

Abstract

Validation of colchicine analysis methods in herbal infusions has been carried out to facilitate quality control of raw materials or simplicia. The validation parameters of the method set include selectivity, linearity, precision, and accuracy, which are included in category I. In this study, the raw material was prepared from the leaves of Gloriosa superba Linn. infusion made. The results of the selectivity test with colchicine as a standard provide uptake at a chosen wavelength of 340.2 nm. Linearity test results show the equation y = 0.0303x + 0.0131 with r = 0.9998> r Table for n = 6, ie 0.917 for p <0.01 and 0.811 for p = <0.05, the value of Vxo is 1 15%. In the precision test, the coefficient of variation is 0.54% and the accuracy test gives an average recovery of 92.81% ± 4.16%. Determination of colchicine in the infusion preparation was carried out as many as three replications. The results showed that the content of colchicine in raw materials was 0.3152% ± 1.99%, while the colchicine content in herbal infusion preparations was 0.483 ± 4.47%.
Inhibitory Activity of Probiotic Milk Against Eschericia coli ATCC 6538 and Staphylococcus aureus ATCC 8739 Andyanita Hanif Hermawati; Aryati Aryati; Isnaeni Isnaeni
Berkala Ilmiah Kimia Farmasi Vol. 7 No. 1 (2020): JUNI
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (549.674 KB) | DOI: 10.20473/bikfar.v7i1.29059

Abstract

The aim of this research is to evaluate inhibitory activity of Lactobacillus acidophilus (L.a) and Lactobacillus casei (L.c) probiotic milk against Escherichia coli and Staphylococcus aureus growth. The fermented milk was prepared by inoculating each probiotic in milk at 43ºC and then incubated at room temperature for 24 hours. Diffusion agar method by using nutrient agar media was used to assess the minimum inhibition concentration (MIC). Obtained results showed pH of fermentation probiotic milk 5 + 0,05. Optimum inhibition zone diameter of L. acidophilus fermented milk against Escherichia coli and Staphylococcus aureus at MIC value of 14 mm. Inhibition zone diameter of L. casei fermented milk obtained an optimum against Escherichia coli and Staphylococcus aureus at MIC value of 12 mm and 13 mm respectively. The L. acidophilus and Lactobacillus casei probiotics fermented milk exhibited considerable antibacterial agent against Escherichia coli and Staphylococcus aureus.
Inhibitory Activity of Fermented Milk Multi Strain Lactic Acid Bacteria Against Staphylococcus Aureus ATCC 25923 Ambarini Indah; Noor Erma Nasution; Achmad Toto Poernomo; Isnaesi Isnaeni
Berkala Ilmiah Kimia Farmasi Vol. 7 No. 1 (2020): JUNI
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (280.59 KB) | DOI: 10.20473/bikfar.v7i1.29064

Abstract

The aim of the study was to investigate inhibitory activity of fermented milk multi strain lactic acid bacteria (LAB): Lactobacillus casei Shirota, Lactobacillus acidophilus, and Lactobacillus bulgaricus against Staphylococcus aureus ATCC 29523 at variable concentration milk using diffusion method. The multi strain culture of LAB were inoculated on skimmed and diary cow milk (DCM) at concentration of 50% and 100%; in which carbon, amino acid, fat and water concentration were different. Effect of the milk and its concentration on the inhibitory activity against the test bacteria was observed as well as decreasing milk acidity and increasing of milk viscosity after 24 hours incubation. Results showed that the performance of LAB in two kinds of milks at 50% and 100% concentrations were different. On the lower milk concentration, the acidity was lower than on the 100% milk, while on the higher milk concentration, the viscosity was bigger than on the 50% concentration. The fermented milk of L. cassei Sirota did not show inhibitory activity. The multi strain of LAB on the DCM at concentration of 50% and 100% showed antibacterial activity againts Staphylococcus aureus ATCC 25923, while the skimmedmed milk fermentation showed lower inhibitory activity on both concentrations.
Anti-bacterial activity of Rosela Flower Extract (Hibiscus sabdariffa L.) against Extended-Spectrum Beta-Lactamase (ESBL) Eschericia coli Muhammad Mu’amar Fathoni; Isnaeni Isnaeni; Asri Darmawati
Berkala Ilmiah Kimia Farmasi Vol. 8 No. 1 (2021): June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (376.754 KB) | DOI: 10.20473/bikfar.v8i1.31204

Abstract

Roselle (Hibiscus sabdariffa L.) contains cyanidin-3-rutinoside, delphinidin, delphinidin-3-monoglucoside, cyanidin-3-monoglucoside, cyanidin-3-sambubioside, cyanidin-3,5-diglucoside may inhibit the growth of Extended-Spectrum-Beta-Lactamase (ESBL) Escherichia coli, but there is no research reported the determination of MIC of Roselle (Hibiscus sabdariffa L.) extract powder against ESBL E.coli ATCC 6110 and ATCC 5949 and their potency ratio compared to meropenem. This study aimed to determine the MIC of Roselle flower extract powder on the growth of ESBL E. coli ATCC 6110 and ATCC 5949 and determine the potency ratio compared to meropenem. This study used two methods in determining MIC, namely the agar diffusion method and the dilution method with Nutrient Agar media which was incubated at 37 ±1°C for 24 hours, while the determination of the potency ratio was carried out by diffusion method with the same media which was incubated at 37 ±1°C for 24 hours. The results obtained were diameter of inhibition zone (mm) which were then observed and analyzed to calculate the potency ratio. The results showed that the MIC of Roselle flower extract powder was obtained at a concentration of 12,500 ppm by diffusion method and at a concentration of 3,125 ppm by dilution method with 24 hours incubation at 37 ± 1o C and the potency ratio of Roselle flower extract powder compared to meropenem was 89.7% and 97.97% against ESBL E.coli ATCC 6110 and ESBL E.coli ATCC 5949 respectively.
Co-Authors A'yun, Arini Qurrata Achmad Toto Poernomo Aditya Fridayanti Aditya Fridayanti Aditya Fridayanti Alief Putriana Rahman Alimsardjono, Lindawati Ambarini Indah Andri Astuti Andyanita Hanif Hermawati Anggita Mirzautika Aprelita Nurelli Dwiana Arie Ika Susanty Aryati Aryati Asri Darmawati Astrid Aulia Hamida Dahlang Tahir Diyah, Nuzul Wahyuning Djoko Agus Purwanto Dwi Riani Oktavia Palupi Esti Handayani Esti Hendradi Febri Annuryanti Fridayanti, Aditya Hadi Poerwono Hana Sofiana Maghfira Hanifah Ridha Rabbani Hepriyadi, Selvy Uftovia I Gede Edy Sagitha Idha Kusumawati Ifah Yulistyani Izza Rahmi Hidayah Juni Ekowati Kholis Amalia Nofianti Laili Irfanah Lisa Nathalie Luky Hayuning Les Mahfudz Mahfudz Marcellino Rudyanto Mega Ferdina Suwito Meliana Susanti Muhammad Agus Syamsur Rijal Muhammad Muslich Muhammad Mu’amar Fathoni Muhammad Yuwono Muhammad Yuwono Muhammad Yuwono Muhammad yuwono Nailatul Hidayah Ni Made Mertaniasih Nindya Pramesti Wardani Noor Erma Nasution Noor Erma Nasution Noor Erma Nasution Noor Erma Nasution Sugijanto Noor Erma NS Nuril Fikriyah Nurrosyidah, Iif Hanifa Primadi Avianto Pujianti, Efrin Pujianti, Efrin Putri, Radha Hartina Ramlan, Nurul Mutmainnah Riesta Primaharinastiti Rifaatul Mahmudah Siswandono, Siswandono Sonia Khoirun Nisa Sri Poedjiarti Sudjarwo Sudjarwo Sugijanto Sugijanto Sugijanto Randiman Sugiyartono, . Sugiyartono, . Suharjono Suharjono Suharjono, Suharjono Sukardiman Suko Hardjono Susanti Susanti Suzana Syahrani, Achmad Syamsul Arifin Tri Sundari Utari Ardiningdyah Widji Soeratri Yulistiani Yulistiani Zahratus Silmi Aliyah