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All Journal Pharmaciana: Jurnal Kefarmasian Jurnal Kedokteran Indonesia Omega: Jurnal Fisika dan Pendidikan Fisika JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Panrita Abdi - Jurnal Pengabdian pada Masyarakat Jurnal Farmasi Sains dan Komunitas Health Notions Warta Pengabdian JFIOnline Pharma Xplore : Jurnal Sains dan Ilmu Farmasi Jurnal Abdi: Media Pengabdian Kepada Masyarakat Jurnal Layanan Masyarakat (Journal of Public Service) Berkala Ilmiah Kimia Farmasi Berkala Penelitian Hayati Journal of Tropical Pharmacy and Chemistry

Isnaeni

Departemen Ilmu Kefarmasian Fakultas Farmasi Universitas Airlangga, Indonesia

Author-ID : 3194825

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Dentistry Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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Effect of sugar cane molasses and tofu waste on the inhibitory activity of cell free fermentation broth of streptomyces antibioticus K-6 Astrid Aulia Hamida; Noor Erma Nasution; Isnaeni Isnaeni
Pharmaciana Vol 9, No 2 (2019): Pharmaciana
Publisher : Universitas Ahmad Dahlan

The use of waste as source of nutrition for human, animals, plants, and microorganism has been reported. The aim of this study was to observe the influence of sugar cane molasses (SCM) and tofu waste (TW) in various concentrations on the inhibitory effect of cell free fermentation broth (CFFB) of Streptomyces antibioticus K-6 isolated from plantationÂ soil compareÂ to International Streptomyces Project (ISP) standardÂ media. The fermentation was performed in 150 rpm rotary shaker at 37ÂºC for five days. TheÂ inhibitory activity wasÂ investigated using diffusion agar on the nutrient agar media to determine ratio of Â SCM and TW by which the largest growth inhibitory zone achieved. Escherichia coli ATCC 25922 was used as a test microorganism. 10% of Streptomyces antibioticus K-6 starter was inoculated into nine compositions Â of Â SCM, TW and its combination containing media. The result indicated that SCM and TWmight be used as the component of fermentation medium of Streptomyces antibioticus K-6 for producing active metabolites. The activity of Â CFFB was exhibited as a diameter of growth Â inhibitoryÂ zone Â against the Â test Â bacteria; in which the largest value (24.4 mm) was detected in the combination of 0.5% SCM and 0.5% TW containig medium after Â two days Â incubation.

Effects of carbon and nitrogen sources on the antibacterial activity of Bacillus tequilensis BSM-F symbiotic with Halichondria panicea sponge from the Cabbiya Coast, Madura, Indonesia Achmad Toto Poernomo; Sonia Khoirun Nisa; Zahratus Silmi Aliyah; Isnaeni Isnaeni
Pharmaciana Vol 10, No 2 (2020): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Sponges form obligate symbiotic associations with antibiotic-producing microorganisms. Many factors, including climate change, anthropogenic pollution, introduced species, and nutrient enrichment, have all been linked to the specific character of marine-symbiotic microorganisms. This recent study investigated the effects of carbon and nitrogen sources on the antibacterial activity of Bacillus tequilensis BSM-F in solid fermentation media, Zobell Marine Agar (ZMA), ZMA was added with various carbon and nitrogen sources at different concentrations to evaluate the effect of enrichment media on the antibacterial activity. The carbon sources used were glucose, glycerol, lactose, and starch, while the nitrogen sources were beef extract, soybean meal, malt extract, and casein. Each of which was added to ZMA at 0.5%, 1%, 1.5%, and 2% w/v. The inhibitory activity was denoted as the activity index, i.e., the ratio of the diameter of zone of inhibition to the diameter of the bacterial colony. B. tequilensis BSM-F exhibited the highest inhibitory activity against Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922 when cultured in media containing glycerol and casein. The optimum condition was achieved when glycerol and casein were each added to ZMA at 1.5% w/v.

Determination and stability testing method of chlorpheniramine maleate in the presence of tartrazine using HPLC Asri Darmawati; Febri Annuryanti; Riesta Primaharinastiti; Isnaeni Isnaeni
Pharmaciana Vol 10, No 3 (2020): Pharmaciana
Publisher : Universitas Ahmad Dahlan

The single-component CPM tablet mostly used sodium tartrazine as the yellow coloring agent. Sodium tartrazine is soluble in solvents used to extract CPM from tablet and suspected interference CPM determination especially after forced degradation for stability indication testing of CPM tablets. This study aimed to develop a selective, accurate and precise method for determination and stability testing of chlorpheniramine maleate (CPM) in the presence of tartrazine in the tablet. A ÂµBondapakÂ® C18 column (3.9 x 300 mm, 10 Âµm) with diode array detector was used for separation. The mobile phase was a mixture of methanol and 0.2% triethylamine (90:10) with a flow rate of 2 mL/minutes. The validated HPLC method was used for CPM determination in tablet samples that had been forced degraded using dry heat at 105oC, UV radiation of 254 nm, hydrolysis with 1N NaOH, 1N HCl and oxidation using 5% H2O2. The HPLC chromatogram showed that CPM split into chlorpheniramine (CP) and maleic acid (MA). Resolution (Rs) among CP and the other analytes especially with the products resulting from the forced degradation by heat, UV radiation, HCl, and H2O2 were good. The CPM hydrolysis using NaOH caused the CP not completely separated from the degradation product due to tailing or overlapping peaks. The proposed HPLC method was valid for the determination of CPM in tablets containing tartrazine. Even though the stability-indicating method was inadequate especially for the result of the CPM hydrolysis process using NaOH.Â

Validation methods for evaluation of ceiba honeyâ€™s growth inhibitory activity against Bacillus subtilis ATCC 6633 Nuzul Wahyuning Diyah; Laili Irfanah; Isnaeni Isnaeni
Pharmaciana Vol 10, No 1 (2020): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Agar diffusion and turbidimetry were commonly used to evaluation ofÂ Â antibacterial activity of honey. However, there is no report about which one of these two methods is better. This study attempts to validate the agar diffusion method and turbidimetry used for growth inhibitory assay of ceiba honey against Bacillus subtilis ATCC 6633 based on validation parameters including Limit of Detection (LOD), linearity, precision, and selectivity. The samples were ceiba honey aqueous solutions in concentration 20%-100%. It was found that the minimum inhibitory concentration (MIC) of agar diffusion and turbidimetric methods were 35% and 30% respectively, considered as LOD. In agar diffusion method,Â plot of inhibitory zone diameter against log of honey concentration yields linear regression equation with r = 0.9804 and Vx0=1.06%, whileÂ rÂ andÂ Vx0of linear plot fromÂ %Â transmittanceÂ againstÂ log concentration inÂ the turbidimetric method were 0.9748 and 1.24% respectively. The coefficient of variation (CV) in agar diffusion method were 1.78% for repeatability and 3.13% for intermediate precision) whereas CV in turbidimetric were 3.64% and 4.05% respectively. Agar diffusion and turbidimetric methods were selective because different source of ceiba honey could give different response in term of MIC. It can be concluded that the agar diffusion and turbidimetric method were valid and suitable for growth inhibitory assay of ceiba honey against Bacillus subtilis ATCC 6633 and there was no significant differences between these methods. The turbidimetry was more sensitive than the agar diffusion method because of its lower LOD and it has more simple experimental technique.

Efek Kondisi Lingkungan Kultur terhadap Produksi Amilase Termostabil oleh Bacillus sphaericus AK-1 Tanah Api Kayangan Bojonegoro Jawa Timur Achmad Toto Poernomo; Isnaeni Isnaeni
JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Vol. 4 No. 1 (2017): Jurnal Farmasi dan Ilmu Kefarmasian Indonesia
Publisher : Universitas Airlangga

Pendahuluan: Produksi amilase telah diteliti menggunakan bakteri yang diisolasi dari tanah Api Kayangan Bojonegoro Jawa Timur. Tujuan: Penelitian ini bertujuan mengisolasi dan identifikasi bakteri dan menggetahui efek kondisi kultur pada aktivitas amilase. Metode: Bakteri dikulturkan pada media yang mengandung pati terlarut sebagai sumber karbon tunggal. Penambahan kalsium (15 mM) atau ekstrak yeast (0,5%) dan pepton (2%) ke media pati terlarut dan mineral akan mengurangi waktu fase lag dan memperpanjang pertumbuhan dan produksi amilase. Pemberian glukosa pada kultur mengurangi produksi amilase, sehingga menunjukkan bahwa efek glukosa berpengaruh pada organisme ini. Hasil: pH media awal dan suhu optimum pada produksi amilase oleh organisme masing-masing 7,0 dan 50°C. Suhu dan pH optimal untuk aktivitas masing-masing 50°C dan 6,0. Larutan enzim dipertahankan aktivitasnya 100% saat diinkubasi pada suhu 90°C selama satu jam dan 40% pada suhu 60°C selama 24 jam. Kesimpulan: Pemberian glukosa pada kultur akan menurunkan produksi amilase.

Validation of Thin-Layer Chromatography-Bioautographic Method for Determination of Streptomycin Isnaeni Isnaeni; Andri Astuti; Muhammad Yuwono
JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Vol. 4 No. 1 (2017): Jurnal Farmasi dan Ilmu Kefarmasian Indonesia
Publisher : Universitas Airlangga

Background: A simple bio-assay for determination of streptomycin hyphenated with planar chromatography techniques was developed. Objective: This study aims to validate the method for identification and determination of streptomycin in injection preparations with TLC-bioautography. Methods: Thin Layer Chromatography (TLC) was performed on the silica Gel GF-254 using KH2PO4 solution as mobile solvent. The visualization was performed by spraying 2% resorcinol. Direct bi autography was developed using Escherichia coli ATCC 25922 as a bacterial test, grown on the nutrient agar medium at 37oC for 24 hours. The method was validated corresponding to linearity, limit of detection (LOD), intra day precision, and accuracy parameters. The accuracy was measured using streptomycin injection as a sample. Results: The Results showed that the KH2PO4 solution at 7.5% concentration was found to be the optimized solvent with Rf value of 0.5. The linear equation was y = 10.176x + 4.046 at 150 - 350 µg/mL concentration range with the linearity coefficient, Limit of Detection, accuracy, and variation coefficient were 0.9907; 40 ppm; 96.37 + 2.22% (with an RSD value of 2.31%); and 1.63 respectively. Conclusion: The prospective TLC-bioautographic method was applied for the identification and determination of streptomycin in a preparation using a single eluent KH2PO4. The eluent system optimization remains necessary for the identification and determination of the mixture of streptomycin with other antibiotics, such as aminoglycoside groups.

Inhibitory Activity of Levofloxacin against MDR Staphylococcus aureus and Pseudomonas aeruginosa Clinical Isolates Lisa Nathalie; Lindawati Alimsardjono; Isnaeni Isnaeni
JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Vol. 6 No. 1 (2019): JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA
Publisher : Universitas Airlangga

Introduction: Staphylococcus aureus and Pseudomonas aeruginosa are the most dangerous and important species among their genus. These bacteria are often resistant to many classes of antimicrobial agents; which make difficulties in selecting appropriate drug to treat infections. Multidrug-resistance occurs readily in hospitals for which antimicrobials agents were used widely. Objective: The aims of this study was to determine minimum inhibitory concentration (MIC) and minimum bacterial concentration (MBC) of levofloxacin against 22 multidrug resistant- clinical (MDR) strains of Staphylococcus aureus and Pseudomonas aeruginosa isolated from patients pus and urine in hospital. Methods: Determination of the MIC was performed by macro-dilution broth assay as recommended by Clinical and Laboratory Standards Institute (CLSI), while the MBC was determined one-step further after the MIC determination. Results: It was found that MIC of the levofloxacin were (0.3 ± 0.0) - >0.5 µg/mL and (0.2 ± 0.1) - (1.0 ±0.0)µg/mL against S. aureus from pus and urine, respectively. In addition, higher MICs were yielded against P. aeruginosa, (1.0 ± 0.0) - >8.0 µg/mL and (0.7 ± 0.3) - (3.0 ± 1.2) µg/mL for pus and urine isolates respectively. Similar to MICs, the MBCs against P. aeruginosa were higher than S. aureus, (0.6 ± 0.0) - > 4.0 µg/mL and (0.3 ± 0.0) - >8.0 µg/mL isolated from pus and urine respectively, (2.0 ± 0.6) - > 8.0 µg/mL and (3.0 ± 1.2) - >7.0 µg/mL against P. aeruginosa from pus and urine respectively. Conclusion: The levofloxacin was still susceptible as bacteriostatic against isolates from both body fluids, but not bactericidal towards all isolates.

The Effect of Vitamin C Addition on Epigallocatechin Gallate (EGCG) Stability in Green Tea Solution Alief Putriana Rahman; Djoko Agus Purwanto; Isnaeni Isnaeni
JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Vol. 6 No. 2 (2019): JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jfiki.v6i22019.62-68

Background: Epigallocatechin gallate (EGCG) is the most abundant green tea catechin with a powerful antioxidant effect to prevent cancer cells. EGCG in green tea solution is highly susceptible to degradation, this it is urgent to increase the stability of EGCG by vitamin C addition. Vitamin C can regenerate radical EGCG to be normal EGCG. Objective: This research aim was to determine percent of decreased level of EGCG before and after vitamin C addition. Methods: This paper was focused on enhancing the stability of EGCG by 1 mg (GTVC1), 1.5 mg (GTVC2), 2 mg (GTVC3), 2.5 mg (GTVC4) and 3 mg (GTVC5) of vitamin C addition to 10 g/L of green tea solution concentration. Evaluations of EGCG were conducted at 0 days, 1 day, 2 days, 3 days, and 4 days of storage time. EGCG was analyzed using thin layer chromatography (TLC) densitometry methods. The stability of EGCG was determined by % of decreased EGCG. Results: Percent of loss of EGCG in GT, GTVC1, GTVC2, GTVC3, GTVC4 and GTVC5 after 4 days storage were 19.93%, 10.89%, 21.08%, 18.18%, 28.56%, and 9.76%, respectively. Conclusion: This study showed that in 4 days storage, the decreased level of EGCG in green tea solution with 3 mg of vitamin C addition (GTVC5) was 9.76% which was smaller than green tea solution without vitamin C addition (GT) which EGCG decreasing 19.93%.

Aktivitas Antibakteri dan Stabilitas Sediaan Gel Minyak Atsiri Daun Jeruk Purut (Citrus hystrix folium) Luky Hayuning Les; Isnaeni Isnaeni; Widji Soeratri
JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Vol. 6 No. 2 (2019): JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jfiki.v6i22019.74-80

Pendahuluan: Daun jeruk (Citrus hystrix folium) mengandung minyak esensial 1-1,5%, menunjukkan aktivitas antibakteri. Bahan aktif yang berkontribusi sebagai aktivitas antibakteri mengandung senyawa dengan gugus alkohol dan aldehida yang mendenaturasi protein sel bakteri. Tujuan: mengevaluasi efektivitas dan stabilitas antibakteri gel yang mengandung minyak esensial hasil isolasi dari daun Citrus hystrix terhadap Staphylococcus epidermidis dan Staphylococcus aureus. Metode: Formulasi gel minyak atsiri dibuat menggunakan karbomer 940 sebagai gelling agent. Aktivitas antibakteri gel yang mengandung minyak atsiri dievaluasi terhadap Staphylococcus epidermidis dan Staphylococcus aureus dengan gel yang mengandung 1% klindamisin sebagai kontrol positif dengan metode difusi agar. Hasil: Sediaan Gel efektif terhadap Staphylococcus epidermidis dan Staphylococcus aureus. Diameter rata-rata zona hambatan pertumbuhan sebelum dan sesudah penyimpanan selama 4 minggu adalah 16,75 mm, dan 17,65 mm. Aktivitas hambatan terhadap dua bakteri uji relatif lebih kecil daripada gel klindamisin. Gel memiliki stabilitas fisik yang baik ditunjukkan dengan tidak adanya perubahan pada konsistensi, warna, bau, viskositas dan pH yang diamati selama penyimpanan 4 minggu. Gel menghasilkan warna putih kekuningan dan bau jeruk yang khas, sedangkan gel kontrol menunjukkan warna kuning transparan dan berbau tween. Kesimpulan: Gel minyak atsiri jeruk secara efektif menghambat pertumbuhan Staphylococcus epidermidis dan Staphylococcus aureus, stabil setelah penyimpanan selama 4 minggu, oleh karena itu prospektif untuk dikembangkan sebagai bahan baku untuk gel anti jerawat.

Effect of Guava Powder Addition on Epigallocatechin Gallate (EGCG) Content of Green Tea and Its Antioxidant Activity Hanifah Ridha Rabbani; Djoko Agus Purwanto; Isnaeni Isnaeni
JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Vol. 6 No. 2 (2019): JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jfiki.v6i22019.85-90

Background: Tea (Camelia sinensis) contains polyphenols including epigallocatechin gallate (EGCG) which is acknowledged to have strong antioxidant properties. However, its stability is strongly influenced by environment. In a neutral and alkaline environment, EGCG could undergo degradation and lose its antioxidant property. There are some researches about the effect of combination of green tea and other plants to their antioxidant capacity. Objective: The research aimed to investigate the effect of guava addition to EGCG content of green tea and their antioxidant activity. Methods: The concentration of EGCG was determined by chromatographic analysis using TLC scanner, meanwhile the antioxidant activity was evaluated by its ability in scavenging 2,2-diphenyl-1-picrylhydrazyl (DPPH) using UV-Vis Spectrophotometer. Results: Among all the samples, formula 4 (2 parts of green tea and 3 parts of guava) gave the highest EGCG content (39.03 ± 3.65 mg/g). This was 37.5% higher than the control sample (28.39 ± 2.45 mg/g). Formula 4 also had the best antioxidant activity with IC50 of 1917.32±1.75 ppm, 19% lower than control sample (2356.46 ± 3.16 ppm). Conclusion: The addition of guava powder significantly increased the amount of EGCG in green tea extracts and their antioxidant activity.

Co-Authors A'yun, Arini Qurrata Achmad Toto Poernomo Aditya Fridayanti Aditya Fridayanti Aditya Fridayanti Alief Putriana Rahman Alimsardjono, Lindawati Ambarini Indah Andri Astuti Andyanita Hanif Hermawati Anggita Mirzautika Aprelita Nurelli Dwiana Arie Ika Susanty Aryati Aryati Asri Darmawati Astrid Aulia Hamida Dahlang Tahir Diyah, Nuzul Wahyuning Djoko Agus Purwanto Dwi Riani Oktavia Palupi Esti Handayani Esti Hendradi Febri Annuryanti Fridayanti, Aditya Hadi Poerwono Hana Sofiana Maghfira Hanifah Ridha Rabbani Hepriyadi, Selvy Uftovia I Gede Edy Sagitha Idha Kusumawati Ifah Yulistyani Izza Rahmi Hidayah Juni Ekowati Kholis Amalia Nofianti Laili Irfanah Lisa Nathalie Luky Hayuning Les Mahfudz Mahfudz Marcellino Rudyanto Mega Ferdina Suwito Meliana Susanti Muhammad Agus Syamsur Rijal Muhammad Muslich Muhammad Mu’amar Fathoni Muhammad Yuwono Muhammad Yuwono Muhammad Yuwono Muhammad yuwono Nailatul Hidayah Ni Made Mertaniasih Nindya Pramesti Wardani Noor Erma Nasution Noor Erma Nasution Noor Erma Nasution Noor Erma Nasution Sugijanto Noor Erma NS Nuril Fikriyah Nurrosyidah, Iif Hanifa Primadi Avianto Pujianti, Efrin Pujianti, Efrin Putri, Radha Hartina Ramlan, Nurul Mutmainnah Riesta Primaharinastiti Rifaatul Mahmudah Siswandono, Siswandono Sonia Khoirun Nisa Sri Poedjiarti Sudjarwo Sudjarwo Sugijanto Sugijanto Sugijanto Randiman Sugiyartono, . Sugiyartono, . Suharjono Suharjono Suharjono, Suharjono Sukardiman Suko Hardjono Susanti Susanti Suzana Syahrani, Achmad Syamsul Arifin Tri Sundari Utari Ardiningdyah Widji Soeratri Yulistiani Yulistiani Zahratus Silmi Aliyah

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