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All Journal HAYATI Journal of Biosciences Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Microbiology Indonesia The Journal of Experimental Life Sciences (JELS) Jurnal Mahasiswa Jurusan Teknik Sipil Jurnal Biodjati JKPK (Jurnal Kimia dan Pendidikan Kimia) J-Dinamika: Jurnal Pengabdian Kepada Masyarakat Indonesian Journal of Halal Research JATI (Jurnal Mahasiswa Teknik Informatika) Journal of Multidisciplinary Applied Natural Science Berkala Penelitian Hayati Tri Dharma Mandiri: Diseminasi dan Hilirisasi Riset Kepada Masyarakat (Jurnal Pengabdian Kepada Masyarakat) Rona Teknik Pertanian Journal Of Carbazon Jurnal Sains & Kesehatan Darussalam
Irfan Mustafa
Department Of Biology, Faculty Of Mathematics And Natural Sciences, Universitas Brawijaya, Malang, Indonesia
Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Earth & Planetary Sciences Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Other

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PERANCANGAN SISTEM INFORMASI BERBASIS WEB UNTUK PROSES PENERIMAAN SANTRI BARU DI PONDOK PESANTREN YOUNG TAHFIZH CENTER Mustafa, Irfan; Yulisa Geni, Bias
JATI (Jurnal Mahasiswa Teknik Informatika) Vol. 8 No. 4 (2024): JATI Vol. 8 No. 4
Publisher : Institut Teknologi Nasional Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36040/jati.v8i4.10042

Abstract

Di era digital ini, teknologi informasi mempunyai peran krusial di berbagai bidang pekerjaan, termasuk dalam bidang pendidikan. Seiring dengan perubahan zaman, peran penting teknologi informasi dalam mendukung lembaga pendidikan seperti Pondok Pesantren semakin nyata yaitu dapat meningkatkan efisiensi dan akurasi setiap prosesnya. Ini sangat bermanfaat, di mana tujuannya adalah untuk mendukung kelancaran setiap proses bisnis, seperti mekanisme dalam penerimaan santri baru, sehingga semakin akurat dan efektif. Proses Penerimaan Santri Baru (PSB) di Pondok Pesantren Young Tahfizh Center masih manual dalam pengisian data pribadi, registrasi, seleksi, hingga pengumuman hasil kelulusan. Meskipun telah menjadi rutinitas, sistem manual tersebut tidak terlepas dari tantangan dan keterbatasan, seperti kelambatan, kerumitan, dan risiko kesalahan manusia. Oleh karena itu, dilakukan sebuah penelitian bertujuan agar memudahkan mekanisme dalam penerimaan dan administrasi data calon santri baru dengan menerapkan metode waterfall. Implementasi sistem ini dirancang dengan JavaScript/Node.js, framework Next.js, Tailwind CSS, Express.js, dan MongoDB sebagai database. Hasil penelitian menunjukan bahwa penerapan website ini dapat secara signifikan meningkatkan efisiensi proses penerimaan santri baru, memudahkan administrasi, serta memudahkan panitia PSB dalam mengelola data. Hasil pengujian blackbox menegaskan semua fitur sistem berfungsi baik dan sesuai harapan, dengan semua skenario pengujian berhasil dan berstatus "Ok", menandakan sistem ini efektif dan siap digunakan.
Phytochemical Screening and Antibacterial Activity of Ethanolic Extracts from Delonix regia Against Laboratory Strains of Diarrheal Bacteria Senge, Yeyen Susana; Mustafa, Irfan; Safitri, Anna
JKPK (Jurnal Kimia dan Pendidikan Kimia) Vol 9, No 1 (2024): JKPK (Jurnal Kimia dan Pendidikan Kimia)
Publisher : Program Studi Pendidikan Kimia FKIP Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jkpk.v9i1.85375

Abstract

The present study evaluates the antibacterial efficacy of ethanolic extracts from Delonix regia leaves against diarrhea-inducing bacteria Escherichia coli and Salmonella typhimurium. Preliminary phytochemical screening revealed that D. regia leaves comprise flavonoids, alkaloids, saponins, tannins, phenolics, and terpenoids. Fourier Transform Infrared Spectroscopy (FTIR) analysis identified various functional groups in the D. regia leaf extract, including O-H, C-H, C=O, C=C, C-C, C-O, and C-O-C. Liquid Chromatography-Mass Spectrometry/Mass Spectrometry (LC-MS/MS) analysis further confirmed the presence of 16 distinct compounds comprising amino acids, alkaloids, phenolics, flavonoids, terpenoids, anthraquinones, n-acyl pyrrolidines, and fatty acids. The disc diffusion method (Kirby-Bauer) was employed for the antibacterial tests. The extracts of D. regia leaves at concentrations of 25%, 50%, 75%, and 100% generated inhibition zones measuring 5.26 mm, 6.37 mm, 7.27 mm, and 10.18 mm against E. coli, and 5.05 mm, 7.01 mm, 8.77 mm, and 10.04 mm against S. typhimurium, respectively. The commercial antibiotic ciprofloxacin (a positive control) produced inhibition zones of 30.02 mm for E. coli and 28 mm for S. typhimurium. The negative control, consisting of 10% ethanol, showed no inhibitory effect on bacterial growth. These findings indicate that the ethanolic extract of D. regia leaves possesses antibacterial properties against E. coli and S. typhimurium. It is likely that secondary metabolite compounds, such as flavonoids and phenolics, contribute significantly to the observed antibacterial activity
Modification of Montmorillonite with Chitosan as Mercury Metal Adsorbent Julinawati, Julinawati; Nisa, Salasatun; Fathurrahmi, Fathurrahmi; Mustafa, Irfan; Saleha, Sitti; MZ., Kartika; Suryati, Suryati
Journal of Carbazon Vol 2, No 2 (2024): December 2024
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jocarbazon.v2i2.42230

Abstract

Modification of montmorillonite with chitosan as an adsorbent of mercury (Hg) metal has been carried out. This study aims to determine the characteristics of the adsorbent, the optimum adsorption capacity of Hg metal, and its adsorption isotherm equation. The optimum conditions for Hg metal adsorption are determined by varying the contact time and pH of the solution. Variations in adsorbate concentration are carried out to determine the adsorption isotherm. Characterization of the adsorbent using FTIR shows the presence of -OH, Si-O, Al-O, C-C, and N-H groups. The optimum conditions for mercury metal adsorption by montmorillonite modified with chitosan occur at a contact time of 80 minutes, and a pH of 4. The adsorption process of mercury metal ions using montmorillonite adsorbent modified with chitosan satisfies both isotherm models, namely the Langmuir and Freundlich isotherms with R2 values of 0.971 and 0.967 respectively, and a maximum adsorption capacity of 13.513 mg/g. The regeneration test showed that the adsorbent can be reused for the absorption of mercury metal ions.
Peningkatan Pemahaman Guru Biologi SMA Kota Malang tentang Evolusi Mikroba melalui Teknologi CRISPR dan Mekanisme Resistensi Antibiotik Jatmiko, Yoga Dwi; Suharjono, Suharjono; Ardyati, Tri; Mustafa, Irfan
J-Dinamika : Jurnal Pengabdian Masyarakat Vol 10 No 1 (2025): April
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The implementation of Merdeka Curriculum in high school requires Biology teachers to face new challenges in teaching, especially the topic of microbial evolution. Microbes play an important role as a model in understanding the evolutionary process, including through CRISPR technology (genome editing). In addition, the mechanism of antibiotic resistance is also a clear example of evolution through genetic changes. However, limited access to information and facilities is an obstacle for teachers in developing appropriate learning modules. This service activity aims to improve the insight and competence of Biology teachers related to microbial evolution, through the introduction of CRISPR technology and antibiotic resistance, both in theory and practice. The training began with a pre-training survey on antibiotic use, material delivery and continued with antibiotic sensitivity test practice. Evaluation in the form of pretest and posttest was also given to participants who were categorized on a scale of understanding: very low, low, medium, high, and very high. The training participants had sufficient experience in the use of antibiotics before the training, but their understanding of antibiotics still varied. The pretest results obtained an understanding of 8.3% very low, 20.8% low, 33.3% medium, 33.3% high, and 4.2% very high. The posttest results showed a significant difference, with only two categories, 38.1% high and 61.9% very high. This result shows that the participants' understanding has increased by 28.4% with an average pretest score of 62 and posttest score of 87. In addition, participants were also able to conduct antibiotic sensitivity tests and how to interpret the data. The participants' response was very positive, with a satisfaction index score between 94-97 which is classified as very good. The theory and practice obtained are expected to increase teacher innovation in learning for students to understand microbial evolution.
Isolation of Nitrogen-Fixing Bacteria from Rhizosphere of Sugarcane Plantation as Candidates for Biofertilizer Bin yahmed, Altayib; Mustafa, Irfan; Rahayu, Farida
The Journal of Experimental Life Science Vol. 15 No. 1 (2025)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2025.015.01.04

Abstract

Exploration of nitrogen-fixing bacteria as a sustainable alternative to conventional fertilizers is important in agriculture. The study aims to isolate and characterize the potential nitrogen-fixing bacteria (NFB) from soils of several sugarcane fields in East Java. Specifically, the bacteria were isolated from sugarcane fields in Blitar, Ngawi, and Glugur Malang. Soil samples were collected, and NFB were isolated using an Nfb medium. Next, the ability of these isolates to fix nitrogen in a liquid medium was evaluated by measuring the ammonium released after different incubation periods (24, 72, and 120 hours). The study obtained five potential isolates that grew well on the agar medium and changed the medium’s pH. Significant growth and pH increase at the liquid medium were observed across the isolates over time. This rise in pH was not always attributed to the ammonia production by those NFB. Only two isolates released ammonia at detectable concentrations as much as 1.13 mg.L-1 and 1.40 mg.L-1, respectively. Hence, isolates C and D were selected as potential NFB for application in replacing synthetic nitrogen fertilizer. Keywords: biofertilizer, environmental sustainability, nitrogen-fixing bacteria, rhizosphere.
Isolation and Identification of Rhizosphere Bacteria Associated with Taro (Colocasia esculenta L. Schott) as Plant Growth Promoting Rhizobacteria Makitalentu, Feybe; Mustafa, Irfan; Suharjono, Suharjono
The Journal of Experimental Life Science Vol. 12 No. 2 (2022)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2022.012.02.01

Abstract

Taro is one of the food crops that has the potential to maintain food security. It has a high carbohydrate content and nutrients with a low glycemic index value. Taro is widely cultivated with intercropping techniques, especially in Kedungkandang District, Malang City. However, this technique caused a decrease in talas tubers production with only 7-10 tons ha-1. This number was lower than the total optimal production with 20.7 tons ha-1 of taro tubers. Plant Growth Promoting Rhizobacteria (PGPR), one of the most potential biofertilizers, can solve this problem with abilities such as phosphate-solubilizing, production of IAA, and nitrogen fixation. This research aimed to analyze the potency and identify the species of taro rhizosphere bacteria that had the best ability as PGPR agents. Taro rhizosphere bacteria were isolated using Pikovskaya medium and TSA (Tryptic Soy Agar) respectively. The PGPR abilities were evaluated on the phosphate-solubilizing, production of IAA, and nitrogen fixation. A total of 12 isolates for phosphate-solubilizing bacteria and ten isolates of IAA-producing bacteria. The highest concentration of phosphate solubilization was P1 isolate, with the concentration of 6.8 μg.mL-1; while I4 isolate had the highest potency for IAA production with the concentration of 23.11 μg.mL-1. Isolates P1 and I4 were selected for the nitrogen fixation ability test. P1 isolate shows the highest ammonia concentration of 2.52 μg.mL-1, and it was identified as Stenotrophomonas rhizophila with a similarity of 99.91% with Stenotrophomonas rhizophila R2A2 67. The potential isolates can be used as PGPR agents or biofertilizers to increase the production of taro plants.      Keywords: Colocasia esculenta, IAA, Nitrogen, PGPR, Phosphate.
Isolation and Identification of Nitrogen-Fixing Rhizobacteria associated with Cocoa plantation (Theobroma cacao L) as Biofertilizer Agent Nurul Afiyatul Jannah; Mustafa, Irfan; Jatmiko, Yoga Dwi
The Journal of Experimental Life Science Vol. 12 No. 3 (2022)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2022.012.03.01

Abstract

Ringinkembar Village, Sumbermanjing Wetan District, Malang Regency, is one of the centers for cocoa plantations using an organic farming system. However, over time this organic farming system experienced a decrease in fruit production, possibly from soil fertility and biofertilizers that were less available in the soil. This study aims to analyze the nitrogen-fixing ability and identify rhizosphere isolates that excel in nitrogen-fixing obtained from the rhizosphere of cacao (Theobroma cacao L) plant. Bacteria were isolated from the soil surrounding cocoa plant roots and grown on Nfb (Nitrogen free Bromothymol Blue) agar media. The nitrogen-fixing bacteria were analyzed with quantitative and qualitative methods. Six potential nitrogen-fixing isolates were identified based on the 16S rDNA sequence. The total number of isolates obtained from nitrogen-fixing isolation was six isolates. The R3.FN1 isolate showed the highest ammonia index at 0.52 µg.L-1 and was identified as Stenotrophomonas maltophilia KB13 with 99.87% similarity to the 16S rDNA sequence. Keywords: Cocoa, Nitrogen-fixing, Rhizosphere, Stenotrophomonas maltophilia.
Isolation of Chitinolytic Bacteria as Biocontrol Agent for Pathogenic Fungi on Cocoa Fruit in Sumbermanjing District, Malang Sulistyantini, Cahyaning; Mustafa, Irfan; Jatmiko , Yoga Dwi
The Journal of Experimental Life Science Vol. 13 No. 1 (2023)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2023.013.01.05

Abstract

The productivity of cocoa plants in Sumbermanjing District is lower than in other parts of Malang Regency. Diseases like pathogenic fungi are to blame for the low output of cocoa plants. This research aimed to find chitinolytic bacteria that were superior at degrading chitin and limiting the growth of pathogenic fungi. These bacteria were isolated from cocoa garden soil and shrimp shell dumps. The bacteria from soil samples taken from the shrimp shell dump in Ujungpangkah District and the cocoa gardens in Sumbermanjing District were cultivated on an agar medium supplemented with colloidal chitin. The pathogenic fungi were discovered from decaying cocoa pods from Sumbermanjing District cocoa plantations. An antagonist test was used to gauge the inhibitory power, and a semi-quantitative test was used to measure the chitinolytic index. The similarity of 16S rDNA sequences allowed for the identification of superior bacterial isolates. The T2S1 isolates of chitinolytic bacteria had the highest levels of pathogenic fungi inhibition (25.14%, 20.85%, and 25.24%). With a 16S rDNA sequence similarity of 99.77%, Bacillus subtilis C10 was identified as the bacterium T2S1. Keywords: Bacillus subtilis, Pathogenetic fungi, Cocoa, Chitinase, 16s-rDNA.
Soil Properties and Macrofauna Community in a Converted Intensive Rice Field into an Organic Polyculture in Malang Regency, Indonesia: Soil Properties and Macrofauna Community in a Converted Intensive Rice Field Inayah, Durrotul; Mustafa, Irfan; Arisoesilaningsih, Endang
Journal of Tropical Life Science Vol. 14 No. 1 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.01.13

Abstract

Farmers in Malang cultivated rice intensively since 2019 due to water availability but reduce yields. It might also reduce soil productivity and increase pest attacks, so the conversion field to organic polyculture was needed using sorghum and legumes. The research aims were to evaluate soil fauna dynamics and soil properties in the three, six, and 12 months after converting (mac) into organic polyculture. Soil and micro-climate factors were recorded including air temperature (°C), day length (hours), rainfall (mm), water content (%), organic matter content (%), electrical conductivity (mS.m-1), pH, and soil bulk density (g.cm-3). Soil macrofauna was sampled using hand sorting (20 x 20 x 10 cm) with five plots at each field. Identified soil macrofauna was used to determine the density, frequency, Important Value Index (IVI), Shannon-Wiener Diversity Index (H'), Evenness Index (E), Simpson Dominance Index (D), Diversity t-test, and Indicator Species. The Canonical Correspondence Analysis (CCA) was used to analyze the interaction among abiotic factors and macrofauna using PAST 4.05. Results showed that the improvement of soil properties including soil organic matter and soil macrofauna was recorded at 6 mac compared to the intensive rice field, and continuously at 12 mac. The richness, diversity, and evenness of soil macrofauna taxa were higher in the converted field than in the intensive one due to organic polyculture. Moreover, we recorded a better proportion of detritivores and predators in the converted field after 12 months. Based on Indicator Species analysis, the dominant fire ants (Solenopsis sp.) in the intensive rice field might be considered as a potential indicator of unhealthy soil in the intensive rice fields. Whereas in the converted field the dominancy of these ants greatly decreased. We concluded that within 6 months conversion using the organic polyculture improved soil properties.
Different Mangrove Rehabilitation Statuses Effects to Benthic Bacterial Structure Community in the Northern Area of Java Island: Mangrove Rehabilitation to Benthic Bacterial in the Northern Area of Java Island Vidayanti, Viky; Retnaningdyah, Catur; Mustafa, Irfan; Oktaviani, Indah
Journal of Tropical Life Science Vol. 14 No. 2 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.02.15

Abstract

The study proposed identifying the changes in bacterial community and diversity and exploring the potential correlations among sediments parameters and benthic bacterial communities under different mangrove ecosystems rehabilitation statuses. There were three sites: 1. Banyuurip Mangrove Center (BMC) as a restoration site; 2. Pulau Lusi (PL) as a degraded site; and 3. Kampung Blekok (KB) as natural site. The physicochemical parameters and benthic bacteria from the mangrove sediment of the three locations were observed. The data were analyzed statistically to determine the sites' variation and the correlation between parameters. The results found that the physicochemical parameters among restoration sites varied. Soil organic matter (SOM) in natural and restoration sites was significantly higher than in degraded sites. Cation exchange capacity (CEC), calcium and potassium ions, and soil conductivity of the restoration site were considerably higher than the other sites. According to diversity indices and taxa richness, benthic bacteria in the restoration were the most diverse. Proteobacteria dominated in natural and restoration sites; meanwhile, Firmicutes dominated in degraded site. Sulfurovum aggregans was found abundant in natural and restoration sites; meanwhile, Mesobacillus subterraneus was the dominant species in the degraded site. Furthermore, the bacterial taxa richness was positively correlated with SOM and the bacterial diversity was correlated with CEC and conductivity.
Co-Authors Aditya Ragil Suharto Aditya, Brillyan Adriani, Azmalina Aini, Afifah Nur Akmal Djamaan Amin Setyo Leksono Aminudin Afandhi ANDALIA, RIZKI Anisa Zairina Anthoni Agustien Bagyo Yanuwiadi Bin yahmed, Altayib Catur Retnaningdyah Dian Siswanto Endang Arisoesilaningsih Endri Purnomo Eva Arifi Evi Octaviany Farida Rahayu Fathurrahmi Fathurrahmi Gustini Ekowati hardiana Ibrahim, Salam A. Inayah, Durrotul Jatmiko , Yoga Dwi Johannes Marulitua Nainggolan Julinawati, Julinawati Kamila, Nurul Khairul Maghfirah Hasanah Makitalentu, Feybe Marlina Marlina Maulida Hikmaranti Maulidiyah, Hikmah Muhamad Imam MZ., Kartika Nadia, Eka Nisa, Salasatun Nurul Afiyatul Jannah Octaviany, Evi Oktaviani, Indah Rahmi Rahmi Rodiyati Azrianingsih Safitri, Anna Saiful Saiful Salfauqi Nurman, Salfauqi Senge, Yeyen Susana Siska Aditya Sitti Saleha, Sitti Suharjono Suharjono Suharjono, Suharjono Sulistyantini, Cahyaning Suryani, Elsa Mega Suryati Suryati Syamsuardi Syamsuardi Syukria Ikhsan Zam Tri Ardyati Tri Ardyati Turhadi Turhadi Vidayanti, Viky Yoga Dwi Jatmiko YOGA DWI JATMIKO yulisa geni, bias Zakaria, Idham Halil