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All Journal HAYATI Journal of Biosciences Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Microbiology Indonesia The Journal of Experimental Life Sciences (JELS) Jurnal Mahasiswa Jurusan Teknik Sipil Jurnal Biodjati JKPK (Jurnal Kimia dan Pendidikan Kimia) J-Dinamika: Jurnal Pengabdian Kepada Masyarakat Indonesian Journal of Halal Research JATI (Jurnal Mahasiswa Teknik Informatika) Journal of Multidisciplinary Applied Natural Science Berkala Penelitian Hayati Tri Dharma Mandiri: Diseminasi dan Hilirisasi Riset Kepada Masyarakat (Jurnal Pengabdian Kepada Masyarakat) Rona Teknik Pertanian Journal Of Carbazon Jurnal Sains & Kesehatan Darussalam
Irfan Mustafa
Department Of Biology, Faculty Of Mathematics And Natural Sciences, Universitas Brawijaya, Malang, Indonesia
Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Earth & Planetary Sciences Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Other

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Tofu Whey-Based Media for Probiotic Lactiplantibacillus plantarum D4 as a Halal Starter Culture Maulidiyah, Hikmah; Mustafa, Irfan; Jatmiko, Yoga Dwi; Ibrahim, Salam A.
Indonesian Journal of Halal Research Vol 6, No 1 (2024): February
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/ijhar.v6i1.30588

Abstract

In recent years, the development of probiotic-based fermented products with halal status has been a concern. The use of growth medium has relied on de Man Rogosa and Sharpe (MRS) as a relatively expensive standard medium, and its halal status is still uncertain. Extensive research has been carried out to investigate the development of low-cost halal alternative media for the cultivation of probiotic lactic acid bacteria (LAB). This study aimed to develop a probiotic halal and low-cost culture medium using a tofu whey-based medium. This study used three tofu whey-based media – A (tofu whey 100%), B (tofu whey 94.5%, molasses 3%, skim milk 2.5%), C (tofu whey 92.5%, molasses 3%, cheese whey 2.5%, tomato extract 2%), and MRS broth as a standard medium. Bacterial populations, total sugars utilized, total lactic acids produced, low pH (2.0) tolerance, and high bile salt concentration (oxgall 1.5%) were assayed. The highest bacterial population after 48 h of incubation was shown by medium B compared to medium MRS (12.34 ± 0.87 and 11.48 ± 0.3 log CFU/mL). Total sugars utilized by 48.28 ± 2.89, 38.89 ± 6.94, 39.14 ± 4.24, and 76.00 ± 1.41 %; on the other hand, total lactic acids produced by 0.16 ± 0.12, 0.03 ± 0.04, 0.31 ± 0.03, and 2.25 ± 1.48 % in A, B, C, and MRS, respectively. Probiotic tolerance at low pH and the presence of bile salts of Lactiplantibacillus plantarum D4 consistently showed a high survival rate in medium B compared to MRS. Based on these results, the components and proportions used in medium B were suitable for the growth of L. plantarum D4 as a halal probiotic starter candidate.
Characterization of Microplastic Degrading Indigenous Bacteria from Ambon Bay Waters Zakaria, Idham Halil; Siswanto, Dian; Mustafa, Irfan
HAYATI Journal of Biosciences Vol. 31 No. 5 (2024): September 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.5.867-879

Abstract

Microplastic degradation by bacteria can degrade low-density polyethylene (LDPE). This study aimed to analyze the potential of Ambon Bay bacteria for microplastic degradation, the condition of microplastics after degradation, and identification of the potential for microplastic degradation. The results of isolation revealed as many as 20 bacterial isolates, which correlated with physicochemical conditions in the waters of Ambon Bay. Nine of them could degrade microplastics as indicated by the presence of a clear zone, namely KA1, KA2, KA3, KA4, KA5, KA9, KA10, KS6, and KS8. They were checked for biofilm formation, microplastic hydrophobicity, and percentage of microplastic weight reduction. Four isolates with the highest percentage of microplastic weight reduction on day 40 were KA1, KA2, KA3, and KA10 at 36.19%, 10.16%, 28.39%, and 17.07%, respectively. The results of LDPE microplastic degradation showed differences using field emission scanning electron microscopy-energy dispersive spectroscopy (FESEM/EDS), attenuated total reflection-fourier transform infrared (ATR-FTIR), and X-ray diffraction (XRD). The bacterial isolates identified were KA1 (Bacillus cereus), KA2 (Bacillus toyonesis), KA3 (Bacillus paramycoides), and KA10 (Escherichia coli). Indigenous bacteria from the waters of Ambon Bay have the potential to degrade LDPE microplastics, which causes structural changes, decreased crystallinity, weight, and C=C groups in microplastics after degradation, with bacterial isolate KA1 identified as Bacillus cereus showing the best potential with degradation of LDPE microplastics by 36.19%.
PERANCANGAN SISTEM INFORMASI BERBASIS WEB UNTUK PROSES PENERIMAAN SANTRI BARU DI PONDOK PESANTREN YOUNG TAHFIZH CENTER Mustafa, Irfan; Yulisa Geni, Bias
JATI (Jurnal Mahasiswa Teknik Informatika) Vol. 8 No. 4 (2024): JATI Vol. 8 No. 4
Publisher : Institut Teknologi Nasional Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36040/jati.v8i4.10042

Abstract

Di era digital ini, teknologi informasi mempunyai peran krusial di berbagai bidang pekerjaan, termasuk dalam bidang pendidikan. Seiring dengan perubahan zaman, peran penting teknologi informasi dalam mendukung lembaga pendidikan seperti Pondok Pesantren semakin nyata yaitu dapat meningkatkan efisiensi dan akurasi setiap prosesnya. Ini sangat bermanfaat, di mana tujuannya adalah untuk mendukung kelancaran setiap proses bisnis, seperti mekanisme dalam penerimaan santri baru, sehingga semakin akurat dan efektif. Proses Penerimaan Santri Baru (PSB) di Pondok Pesantren Young Tahfizh Center masih manual dalam pengisian data pribadi, registrasi, seleksi, hingga pengumuman hasil kelulusan. Meskipun telah menjadi rutinitas, sistem manual tersebut tidak terlepas dari tantangan dan keterbatasan, seperti kelambatan, kerumitan, dan risiko kesalahan manusia. Oleh karena itu, dilakukan sebuah penelitian bertujuan agar memudahkan mekanisme dalam penerimaan dan administrasi data calon santri baru dengan menerapkan metode waterfall. Implementasi sistem ini dirancang dengan JavaScript/Node.js, framework Next.js, Tailwind CSS, Express.js, dan MongoDB sebagai database. Hasil penelitian menunjukan bahwa penerapan website ini dapat secara signifikan meningkatkan efisiensi proses penerimaan santri baru, memudahkan administrasi, serta memudahkan panitia PSB dalam mengelola data. Hasil pengujian blackbox menegaskan semua fitur sistem berfungsi baik dan sesuai harapan, dengan semua skenario pengujian berhasil dan berstatus "Ok", menandakan sistem ini efektif dan siap digunakan.
Phytochemical Screening and Antibacterial Activity of Ethanolic Extracts from Delonix regia Against Laboratory Strains of Diarrheal Bacteria Senge, Yeyen Susana; Mustafa, Irfan; Safitri, Anna
JKPK (Jurnal Kimia dan Pendidikan Kimia) Vol 9, No 1 (2024): JKPK (Jurnal Kimia dan Pendidikan Kimia)
Publisher : Program Studi Pendidikan Kimia FKIP Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jkpk.v9i1.85375

Abstract

The present study evaluates the antibacterial efficacy of ethanolic extracts from Delonix regia leaves against diarrhea-inducing bacteria Escherichia coli and Salmonella typhimurium. Preliminary phytochemical screening revealed that D. regia leaves comprise flavonoids, alkaloids, saponins, tannins, phenolics, and terpenoids. Fourier Transform Infrared Spectroscopy (FTIR) analysis identified various functional groups in the D. regia leaf extract, including O-H, C-H, C=O, C=C, C-C, C-O, and C-O-C. Liquid Chromatography-Mass Spectrometry/Mass Spectrometry (LC-MS/MS) analysis further confirmed the presence of 16 distinct compounds comprising amino acids, alkaloids, phenolics, flavonoids, terpenoids, anthraquinones, n-acyl pyrrolidines, and fatty acids. The disc diffusion method (Kirby-Bauer) was employed for the antibacterial tests. The extracts of D. regia leaves at concentrations of 25%, 50%, 75%, and 100% generated inhibition zones measuring 5.26 mm, 6.37 mm, 7.27 mm, and 10.18 mm against E. coli, and 5.05 mm, 7.01 mm, 8.77 mm, and 10.04 mm against S. typhimurium, respectively. The commercial antibiotic ciprofloxacin (a positive control) produced inhibition zones of 30.02 mm for E. coli and 28 mm for S. typhimurium. The negative control, consisting of 10% ethanol, showed no inhibitory effect on bacterial growth. These findings indicate that the ethanolic extract of D. regia leaves possesses antibacterial properties against E. coli and S. typhimurium. It is likely that secondary metabolite compounds, such as flavonoids and phenolics, contribute significantly to the observed antibacterial activity
Modification of Montmorillonite with Chitosan as Mercury Metal Adsorbent Julinawati, Julinawati; Nisa, Salasatun; Fathurrahmi, Fathurrahmi; Mustafa, Irfan; Saleha, Sitti; MZ., Kartika; Suryati, Suryati
Journal of Carbazon Vol 2, No 2 (2024): December 2024
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jocarbazon.v2i2.42230

Abstract

Modification of montmorillonite with chitosan as an adsorbent of mercury (Hg) metal has been carried out. This study aims to determine the characteristics of the adsorbent, the optimum adsorption capacity of Hg metal, and its adsorption isotherm equation. The optimum conditions for Hg metal adsorption are determined by varying the contact time and pH of the solution. Variations in adsorbate concentration are carried out to determine the adsorption isotherm. Characterization of the adsorbent using FTIR shows the presence of -OH, Si-O, Al-O, C-C, and N-H groups. The optimum conditions for mercury metal adsorption by montmorillonite modified with chitosan occur at a contact time of 80 minutes, and a pH of 4. The adsorption process of mercury metal ions using montmorillonite adsorbent modified with chitosan satisfies both isotherm models, namely the Langmuir and Freundlich isotherms with R2 values of 0.971 and 0.967 respectively, and a maximum adsorption capacity of 13.513 mg/g. The regeneration test showed that the adsorbent can be reused for the absorption of mercury metal ions.
Peningkatan Pemahaman Guru Biologi SMA Kota Malang tentang Evolusi Mikroba melalui Teknologi CRISPR dan Mekanisme Resistensi Antibiotik Jatmiko, Yoga Dwi; Suharjono, Suharjono; Ardyati, Tri; Mustafa, Irfan
J-Dinamika : Jurnal Pengabdian Masyarakat Vol 10 No 1 (2025): April
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The implementation of Merdeka Curriculum in high school requires Biology teachers to face new challenges in teaching, especially the topic of microbial evolution. Microbes play an important role as a model in understanding the evolutionary process, including through CRISPR technology (genome editing). In addition, the mechanism of antibiotic resistance is also a clear example of evolution through genetic changes. However, limited access to information and facilities is an obstacle for teachers in developing appropriate learning modules. This service activity aims to improve the insight and competence of Biology teachers related to microbial evolution, through the introduction of CRISPR technology and antibiotic resistance, both in theory and practice. The training began with a pre-training survey on antibiotic use, material delivery and continued with antibiotic sensitivity test practice. Evaluation in the form of pretest and posttest was also given to participants who were categorized on a scale of understanding: very low, low, medium, high, and very high. The training participants had sufficient experience in the use of antibiotics before the training, but their understanding of antibiotics still varied. The pretest results obtained an understanding of 8.3% very low, 20.8% low, 33.3% medium, 33.3% high, and 4.2% very high. The posttest results showed a significant difference, with only two categories, 38.1% high and 61.9% very high. This result shows that the participants' understanding has increased by 28.4% with an average pretest score of 62 and posttest score of 87. In addition, participants were also able to conduct antibiotic sensitivity tests and how to interpret the data. The participants' response was very positive, with a satisfaction index score between 94-97 which is classified as very good. The theory and practice obtained are expected to increase teacher innovation in learning for students to understand microbial evolution.
Tofu Whey-Based Media for Probiotic Lactiplantibacillus plantarum D4 as a Halal Starter Culture Maulidiyah, Hikmah; Mustafa, Irfan; Jatmiko, Yoga Dwi; Ibrahim, Salam A.
Indonesian Journal of Halal Research Vol. 6 No. 1 (2024): February
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/ijhar.v6i1.30588

Abstract

In recent years, the development of probiotic-based fermented products with halal status has been a concern. The use of growth medium has relied on de Man Rogosa and Sharpe (MRS) as a relatively expensive standard medium, and its halal status is still uncertain. Extensive research has been carried out to investigate the development of low-cost halal alternative media for the cultivation of probiotic lactic acid bacteria (LAB). This study aimed to develop a probiotic halal and low-cost culture medium using a tofu whey-based medium. This study used three tofu whey-based media - A (tofu whey 100%), B (tofu whey 94.5%, molasses 3%, skim milk 2.5%), C (tofu whey 92.5%, molasses 3%, cheese whey 2.5%, tomato extract 2%), and MRS broth as a standard medium. Bacterial populations, total sugars utilized, total lactic acids produced, low pH (2.0) tolerance, and high bile salt concentration (oxgall 1.5%) were assayed. The highest bacterial population after 48 h of incubation was shown by medium B compared to medium MRS (12.34 ± 0.87 and 11.48 ± 0.3 log CFU/mL). Total sugars utilized by 48.28 ± 2.89, 38.89 ± 6.94, 39.14 ± 4.24, and 76.00 ± 1.41 %; on the other hand, total lactic acids produced by 0.16 ± 0.12, 0.03 ± 0.04, 0.31 ± 0.03, and 2.25 ± 1.48 % in A, B, C, and MRS, respectively. Probiotic tolerance at low pH and the presence of bile salts of Lactiplantibacillus plantarum D4 consistently showed a high survival rate in medium B compared to MRS. Based on these results, the components and proportions used in medium B were suitable for the growth of L. plantarum D4 as a halal probiotic starter candidate.
Isolation of Nitrogen-Fixing Bacteria from Rhizosphere of Sugarcane Plantation as Candidates for Biofertilizer Bin yahmed, Altayib; Mustafa, Irfan; Rahayu, Farida
The Journal of Experimental Life Science Vol. 15 No. 1 (2025)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2025.015.01.04

Abstract

Exploration of nitrogen-fixing bacteria as a sustainable alternative to conventional fertilizers is important in agriculture. The study aims to isolate and characterize the potential nitrogen-fixing bacteria (NFB) from soils of several sugarcane fields in East Java. Specifically, the bacteria were isolated from sugarcane fields in Blitar, Ngawi, and Glugur Malang. Soil samples were collected, and NFB were isolated using an Nfb medium. Next, the ability of these isolates to fix nitrogen in a liquid medium was evaluated by measuring the ammonium released after different incubation periods (24, 72, and 120 hours). The study obtained five potential isolates that grew well on the agar medium and changed the medium’s pH. Significant growth and pH increase at the liquid medium were observed across the isolates over time. This rise in pH was not always attributed to the ammonia production by those NFB. Only two isolates released ammonia at detectable concentrations as much as 1.13 mg.L-1 and 1.40 mg.L-1, respectively. Hence, isolates C and D were selected as potential NFB for application in replacing synthetic nitrogen fertilizer. Keywords: biofertilizer, environmental sustainability, nitrogen-fixing bacteria, rhizosphere.
Isolation and Identification of Rhizosphere Bacteria Associated with Taro (Colocasia esculenta L. Schott) as Plant Growth Promoting Rhizobacteria Makitalentu, Feybe; Mustafa, Irfan; Suharjono, Suharjono
The Journal of Experimental Life Science Vol. 12 No. 2 (2022)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2022.012.02.01

Abstract

Taro is one of the food crops that has the potential to maintain food security. It has a high carbohydrate content and nutrients with a low glycemic index value. Taro is widely cultivated with intercropping techniques, especially in Kedungkandang District, Malang City. However, this technique caused a decrease in talas tubers production with only 7-10 tons ha-1. This number was lower than the total optimal production with 20.7 tons ha-1 of taro tubers. Plant Growth Promoting Rhizobacteria (PGPR), one of the most potential biofertilizers, can solve this problem with abilities such as phosphate-solubilizing, production of IAA, and nitrogen fixation. This research aimed to analyze the potency and identify the species of taro rhizosphere bacteria that had the best ability as PGPR agents. Taro rhizosphere bacteria were isolated using Pikovskaya medium and TSA (Tryptic Soy Agar) respectively. The PGPR abilities were evaluated on the phosphate-solubilizing, production of IAA, and nitrogen fixation. A total of 12 isolates for phosphate-solubilizing bacteria and ten isolates of IAA-producing bacteria. The highest concentration of phosphate solubilization was P1 isolate, with the concentration of 6.8 μg.mL-1; while I4 isolate had the highest potency for IAA production with the concentration of 23.11 μg.mL-1. Isolates P1 and I4 were selected for the nitrogen fixation ability test. P1 isolate shows the highest ammonia concentration of 2.52 μg.mL-1, and it was identified as Stenotrophomonas rhizophila with a similarity of 99.91% with Stenotrophomonas rhizophila R2A2 67. The potential isolates can be used as PGPR agents or biofertilizers to increase the production of taro plants.      Keywords: Colocasia esculenta, IAA, Nitrogen, PGPR, Phosphate.
Isolation and Identification of Nitrogen-Fixing Rhizobacteria associated with Cocoa plantation (Theobroma cacao L) as Biofertilizer Agent Nurul Afiyatul Jannah; Mustafa, Irfan; Jatmiko, Yoga Dwi
The Journal of Experimental Life Science Vol. 12 No. 3 (2022)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2022.012.03.01

Abstract

Ringinkembar Village, Sumbermanjing Wetan District, Malang Regency, is one of the centers for cocoa plantations using an organic farming system. However, over time this organic farming system experienced a decrease in fruit production, possibly from soil fertility and biofertilizers that were less available in the soil. This study aims to analyze the nitrogen-fixing ability and identify rhizosphere isolates that excel in nitrogen-fixing obtained from the rhizosphere of cacao (Theobroma cacao L) plant. Bacteria were isolated from the soil surrounding cocoa plant roots and grown on Nfb (Nitrogen free Bromothymol Blue) agar media. The nitrogen-fixing bacteria were analyzed with quantitative and qualitative methods. Six potential nitrogen-fixing isolates were identified based on the 16S rDNA sequence. The total number of isolates obtained from nitrogen-fixing isolation was six isolates. The R3.FN1 isolate showed the highest ammonia index at 0.52 µg.L-1 and was identified as Stenotrophomonas maltophilia KB13 with 99.87% similarity to the 16S rDNA sequence. Keywords: Cocoa, Nitrogen-fixing, Rhizosphere, Stenotrophomonas maltophilia.
Co-Authors Aditya Ragil Suharto Aditya, Brillyan Adriani, Azmalina Aini, Afifah Nur Akmal Djamaan Amin Setyo Leksono Aminudin Afandhi ANDALIA, RIZKI Anisa Zairina Anthoni Agustien Bagyo Yanuwiadi Bin yahmed, Altayib Catur Retnaningdyah Dian Siswanto Endang Arisoesilaningsih Endri Purnomo Eva Arifi Evi Octaviany Farida Rahayu Fathurrahmi Fathurrahmi Gustini Ekowati hardiana Ibrahim, Salam A. Inayah, Durrotul Jatmiko , Yoga Dwi Johannes Marulitua Nainggolan Julinawati, Julinawati Kamila, Nurul Khairul Maghfirah Hasanah Makitalentu, Feybe Marlina Marlina Maulida Hikmaranti Maulidiyah, Hikmah Muhamad Imam MZ., Kartika Nadia, Eka Nisa, Salasatun Nurul Afiyatul Jannah Oktaviani, Indah Rahmi Rahmi Rodiyati Azrianingsih Safitri, Anna Saiful Saiful Salfauqi Nurman, Salfauqi Senge, Yeyen Susana Siska Aditya Sitti Saleha, Sitti Suharjono Suharjono Suharjono, Suharjono Sulistyantini, Cahyaning Suryani, Elsa Mega Suryati Suryati Syamsuardi Syamsuardi Syukria Ikhsan Zam Tri Ardyati Tri Ardyati Turhadi Turhadi Vidayanti, Viky YOGA DWI JATMIKO Yoga Dwi Jatmiko yulisa geni, bias Zakaria, Idham Halil