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All Journal Jurnal Akuakultur Rawa Indonesia Journal of Aquaculture Science Journal of Aquaculture and Fish Health Grouper: Jurnal Ilmiah Perikanan Journal of Marine and Coastal Science Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan Journal of Fisheries & Marine Jurnal Perikanan
Boedi Setya Rahardja
Departemen Akuakultur, Fakultas Perikanan Dan Kelautan Universitas Airlangga, Surabaya, Jawa Timur, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Engineering Environmental Science Immunology & microbiology Veterinary

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Journal : Journal of Fisheries

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Uji Efektifitas Ekstrak Daun Sirih Merah (Piper rocatum) Terhadap Bakteri Aeromonas hydrophila Secara In Vitro [ Effectines Of Leaves Extracts Red Betel (Piper crocatum) Againts Of Bacteria Aeromonas hydrophila In Vitro ] Ratih Kusuma Wardani, Wahyu Tjahjaningsih, Boedi Setya Rahardja
Jurnal Ilmiah Perikanan dan Kelautan Vol. 4 No. 1 (2012): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v4i1.11584

Abstract

Abstract Aeromonas hydrophila is a motile Aeromonas bacteria that cause Septicemia (MAS) or the disease kwon as red spots (Plumb, 1994). These bacteria attack the various types of freshwater fish such as African catfish, carp and prawns. Treatment of MAS is still a lot of use antibiotics, whereas antibiotic a lot side effects. This study tries for find an alternative use  antibacterial substances using natural chemicals derived from medicinal plants of leaves extracs red betel (P. crocatum) which is antibacterial is a volatile oil, flavonoids, alkaloids and tannins.  The purpose of this study was to determine the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of leaves  extracts red betel (P. crocatum) against of bacteria A. hydrophila. The research was conducted on July-August 2011, at the faculty of veterinary medicine, Airlangga University and Organic Chemistry Laboratory Faculty Of science and Technology Airlangga University. The method used in this study is an experimental method, using completely randomized design (RAL) with 11 treatments and three replications. Variables observed in this study is the minimum inhibitory concentration (MIC) and Minimum Bactericidal Concentration (MBC) of leaves extracts red betel (P. crocatum). The observations were analyzed using analysis of variance (ANAVA). The results showed that extracts leaves red betel (P. crocatum) has efficacy against the bacteria A. hydrophila in vitro. Based on the results of MIC testing, leaves exracts of red betel (P. crocatum) inhibits growth of bacteria A. hydrophila at a concentration of 12,5% and based on test results of MBC, leaves extracs of red betel (P. crocatum) effectively kills the bacteria A. hydrophila at a concentration of 25%. It can be concluded that the concentration of antibacterial substances contained in red betel able to inhibit and kill bacteria A. hydrophila. This study recommends further to determine the effects of leaves extracts  red betel (P. crocatum) A. hydrophila against bacteria in vitro, so that can know the effectiveness of leaves effects of leaves extracts  red betel (P. crocatum) in suppressing populations of bacteria A. hydrophila
Uji Potensi Sari Buah Belimbing Wuluh (Averrhoa bilimbi L.) dalam Menghambat Pertumbuhan Bakteri Aeromonas salmonicida Smithia Secara In Vitro [Potential Test Cucumber Fruit Juice (Averrhoa bilimbi L.) In Inhibiting Growth Of Aeromonas salmonicida Smithia Bacteria By In Vitro] Prayogo Prayogo; Boedi Setya Rahardja; Rena Wilis Putri
Jurnal Ilmiah Perikanan dan Kelautan Vol. 3 No. 2 (2011): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v3i2.11602

Abstract

Abstract Giving antibiotics continuously can cause A. salmonicida smithia become resistant and residues of antibiotics may pollute the water environment. One alternative to using traditional medicinal plants that are antibacterial. Advantages of using traditional medicinal plants are relatively more secure, easily available, inexpensive, does not cause resistance, and relatively harmless to the surrounding environment. The content of natural chemicals from cucumber fruit is known to have antibacterial effects namely, flavonoids and phenols. The purpose of this study to determine ability the cucumber fruit juice inhibit bacterial growth A. salmonicida smithia and to determine the best concentration to inhibit bacterial growth A. salmonicida smithia with ampicillin drugs for comparison by in vitro. This research was conducted in the Bacteriology Laboratory of Fish Quarantine Center of Sidoarjo and Human Genetic Laboratory the Institute of Tropical Disease Centre, Airlangga University, Surabaya. This research use done eksperimental method by in vitro, with a large measure of cucumber fruit juice inhibition zone around the existing paper disc and comparing it to a large zone of inhibition using ampicillin. Concentration dilution series cucumber fruit juice used during the research, began 2 gr/ 2 ml to the concentration of 0,0625 gr/ml. Result of research show at concentration 0,125 gr/ml still has ability to pursue bacterium A. smithia salmonicida, so that can be anticipated that effective cucumber fruit juice if used as by drug, because at small concentration can to pursue bacteria A. smithia salmonicida by in vitro.
Daya Antibakteri Sari Buah Majapahit (Crescentia cujete L.) terhadap Bakteri Aeromonas salmonicida Secara In Vitro [Antibacterial Activity of The Juice Majapahit Fruit (Crescentia cujete L.) To Bacteria Aeromonas salmonicida Against In Vitro] Boedi Setya Rahardja; Fitria Yusmita Sari; Yudi Cahyoko
Jurnal Ilmiah Perikanan dan Kelautan Vol. 3 No. 1 (2011): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v3i1.11632

Abstract

Abstract Bacteria are single-celled organisms, reproduce by dividing themselves (asexual) that reproduce by way of extending the cell nucleus followed by cell division called binary fission (Prajitno, 2005) and can only be viewed with a microscope because of their small size (Dwijoseputro, 1989 .) One of the bacteria that cause the greatest failures in the cultivation of freshwater fish and sea water is furunculosis (presence of a number of furuncles at the same time) caused by the bacterium Aeromonas salmonicida (Cipriano and Bullock, 2001). A. salmonicida is divided into several subspecies, among others, A. salmonicida subspecies salmonicida, subspecies masoucida, subspecies achromogenes and subspecies smithia (Hiney and Olivier, 1999). Fish infected with these bacteria will show symptoms such as fish body color becomes darker, the ability to swim down, resulting haemorrhagic septicemia, the abdomen looks bloated, the eyes and slightly protruding damaged fish (exopthalmia), all damaged fins, and gills become pale and damaged (Buller, 2004). The method used in the research is experimental method. This study aims to determine whether fruit majapahit juice (C. cujete L.) has antibacterial power against bacteria A. salmonicida and find out whether or not sensitive majapahit juice (C. cujete L.) in inhibiting the growth of bacteria A. salmonicida with standard comparator antibiotic kanamycin. Research conducted at the Laboratory of Bacteriology Hall Fish Quarantine Airport Highway Ir. H. Juanda Street No.23, Semambung Village Sedati- Sidoarjo District, East Java Province in February 2011. The results showed that the juice majapahit (C. cujete L.) could inhibit bacterial growth of A. salmonicida against in vitro, but when compared with standard antibiotic kanamycin 30 µg still relatively insensitive to inhibit bacterial growth of A. salmonicida. 
Pengaruh Penggunaan Tepung Daging Bekicot (Achatina fulica) pada Pakan Buatan terhadap Pertumbuhan, Rasio, Konversi Pakan dan Tingkat Kelulushidupan Benih Ikan Patin (Pangasius pangasius) [Effect of Use of Snail Meat (Achatina fulica) for Artificial Diet on Growth, Food Convertion and Survival Rate Catfish (Pangasius pangasius)] Boedi Setya Rahardja; Devieta Sari; Moch. Amin Alamsjah
Jurnal Ilmiah Perikanan dan Kelautan Vol. 3 No. 1 (2011): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v3i1.11634

Abstract

AbstractCurrently catfish is experiencing a fairly rapid growth, this is because the catfish is one of the important commodities in the fishery. Efforts to increase catfish production is to improve the quality of feed. The aim of this study is to observe effect of snail meat meal for growth rate, food conversion ratio and survival rate of catfish seeds. This research method is experimental with a Completely Randomized Design (CRD) with five treatments and four replications. The treatments were used: (A) 0% snail meat meal, (B) 10% snail meat meal and 30% fish meal, (C) 20% snail meat meal and 20% fish meal, (D) 30% snail meat meal and 10% fish meal and (E) 40% snail meat meal. The main parameters measured were growth, feed conversion ratio and survival rate. Supporting parameters measured were water quality. Analysis of the data used is Analysis of variance (ANOVA) and to know the difference among treatment using Duncan's Multiple Range Test. The results of this study showed that use of snail meat meal in feed formulation stastistically difference (p<0.05) for growth and survival rate of catfish seeds but not statistically difference (p>0.05) for feed conversion ratio. Water quality in maintenance media seed catfish is the temperature 0range between 26-31C, pH 7-8 and dissolved oxygen was 7.2-8.0 mg/L. 
Pengaruh Pemberian Berbagai Jenis Pakan Terhadap Pertumbuhan Dan Tingkat Kelulushidupan Ikan Silih (Macrognathus aculeatus) [The Effect Of Vary Feed On Growth And Survival Rate Of Spiny Eel (Macrognathus aculeatus)] Akhmad Taufiq Mukti; Denta Batara Kusuma Hendra; Boedi Setya Rahardja
Jurnal Ilmiah Perikanan dan Kelautan Vol. 2 No. 2 (2010): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v2i2.11641

Abstract

Abstract The purpose of this research was to know the effect of kinds of feeding on growth and survival rate of spiny eel. This research used experimental method and Completely Random Design method with six treatment and each treatment was replicated four times. The treatments are : artificial feed (A), nila fish (B), shrimp (C), combination of artificial feed with nila fish (D), combination of artificial feed with shrimp (E) and combination of nila fish with shrimp (F). The main parameter observed are specific growth rate, growth of absolute length and survival rate. The support parameter is water quality. The data analiysis of this research used a Analysis of Varian (ANAVA) and to determine the best performed used Duncan's Multiple Range test. The result of this research showed that given kinds of feeding was not significantly difference (p>0,05) to specific growth rate, growth of absolute length and survival rate of spiny eel. The average value of growth of absolute length, specific growth rate and survival rate of each treatment are treatment C (2,5062 mm), treatment C (0,9375 %BT/hari) and treatment C (71,6495%).
Penambahan Ekstender Madu Dalam Proses Penyimpanan Sperma Beku Terhadap Motilitas Dan Viabilitas Spermatozoa Ikan Komet (Carassius auratus auratus) [Additions Extender Honey In Frozen Sperm Storage Process Against Sperm Motility And Viability Comet Fish (Carassius auratus auratus) ] Boedi Setya Rahardja; A. Shofy Mubarak; Permana Sulistyo Rini
Jurnal Ilmiah Perikanan dan Kelautan Vol. 2 No. 2 (2010): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v2i2.11649

Abstract

Abstract The development of fish culture is strongly influenced by hatchery technology, especially in the stock of fish seed. Often, the problem arises in the stock of seeds, due to the maturation of gametes, broadstock fish sometimes do not happen simultaneously, one of alternative solution to the problem through the application of reproductive biotechnology, is preservation of sperm (Dirjennak, 2007). Preservation sperm is to optimize the male broadstock (Dirjennak, 2007). This storage process requires diluent and cryoprotectant material that could sustain sperm motility (Zaenab, 2007). Diluent material is used to reduce the activity of spermatozoa that inhibit energy usage and prolong the life of sperm (Rustidja, 2000). The use of honey as a diluent material is expected to support the vitality of spermatozoa. Material of honey there are various combinations of materials a simple sugar and salt ions. With the combination of basic ingredients and simple sugar salt ions spermatozoa can produce an energy source so it can defend itself and can fertilize an egg cell after Cryoperservation process. The purpose of this research is to know the effect of adding extender honey in enhancing sperm motility and viability of fish comet after the freezing process and to determine the best dose of honey in the process of freezing sperm comet fish (Carassius auratus auratus). This research was conducted at the Laboratory Center for Artificial Insemination Singosari. The design use Completely Randomized Design (CRD) followed by Duncan's multiple range test. Test materials in this research is sperm comet fish are packed in mini straw and stored in liquid nitrogen container with 9 treatments and 3 replications. Media diluent used was physiological NaCl is added to honey, glucose, fructose, and Tris Aminomethane. Honey dosages of the experiments were 0.3% (A); 0.4% (B), 0.5% (C), 0.6% (D) and 0.7% (E) and 0.05% glucose (KG); fructose 0.05% (KF), Tris Aminomethane (KT) and without the addition of physiological NaCl (KN). The main parameters of observed percentage of live sperm and duration of motion. The supporting parameters measured were fresh sperm concentration, percentage of live sperm fresh, long fresh sperm, pH, volume and color of sperm. The results of the research shows that the addition of honey with different dosage at diluents material physiological NaCl gived significant effect (P <0.05) against the percentage of sperm motion and long life. Average percentage of long life and highest sperm obtained by treatment of the addition of honey 0.6% (D), ie 63.89% and 101.33 seconds. Average lowest percentage of life gained by treatment of honey 0.3% (C) that were 39.67 second while the lowest was in motion treatment control without the addition of (KN) that were 28.33 seconds.
Pengaruh Pemberian Vaksin Whole Cell Killed Virus Terhadap Sintasan Udang Vannamei (Lithopenaeus vannamei) Yang Diinfeksi Whitespot Baculovirus (WSBV) [The Influence Of Whitespot Baculovirus (WSBV) Infection In Lithopenaeus vannamei By Giving Whole Cell Killed Virus Vaccine On Survival Rate.] Hari Suprapto; Angga Bahtera Siswanto; Boedi Setya Rahardja
Jurnal Ilmiah Perikanan dan Kelautan Vol. 2 No. 1 (2010): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v2i1.11670

Abstract

AbstractShrimp are susceptible to a wide variety of pathogens, one of each were viruses. Whitespot Baculovirus (WSBV) is one of virus attack in vannamei. Shrimp had been infected by WSBV will showed high mortality. One of Strategies for prophylaxis and control of WSBV is enhancement of disease resistant by using vaccines. Recently, quasi-immune response have been reported by which Penaeus japonicus surviving from WSBV infections possess a resistance against challenge WSBV (Venegas et al., 2000). This study was used Lithopenaeus vannamei that been vaccination with inactived-formaline WSBV virus cell or it called Whole Cell Killed Virus (WCKV) to know its responses to WSBV. The aim of this study was to know the effect Whole Cell Killed Virus vaccine to survival rate vannamei shrimp (Lithopenaeus vannamei) infected by Whitespot Baculovirus (WSBV). This study used descriptive study. The efficacy of vaccines made of inactivated WSSV with different dose. The dose are P1 (dose 0,01µg/ml each shrimp), P2 (dose 0,02 µg/ml each shrimp), P3 (dose 0,03 µg/ml each shrimp) dan P4 (non-vaccine (kontrol) injected by PBS). Primary parameter was survival rate (%). Secondary parameter was water quality which of temperature, pH, salinity and dissolved oxygen. The result showed that survival rate of L. Vannamei with different dose of WCKV vaccines was increases.
Pengaruh Suhu Dan Kepadatan Ephippia Yang Berbeda Terhadap Penetasan Ephippia Daphnia magna [The Influence Of Defferent Temperature And Ephippia Density For Ephippia Hatch Of Daphnia magna.] Yudi Cahyoko; Yulian Cindra Eka Pradana; Boedi Setya Rahardja
Jurnal Ilmiah Perikanan dan Kelautan Vol. 1 No. 1 (2009): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v1i1.11695

Abstract

Abstract Daphnia magna has good nutrition to carry on egg produce and fry of fish cupang, maskoki, oscar, tetra, and also can used for food source of fry and seed. Daphnia magna not only supply from natural environment, cause their growth deppend on fertilizer as food and environment condition. The other side: high population density, limited of food source low temperature and shorter irradiation can produce dorman egg (ephippia). The dorman eeg can use for Daphnia magna stock which way by hatching. The factor that can be influence for ephippia hatch are temperature and density. Based on this fact, a research about influence of different temperature and ephippia density for ephippia hatch of Daphnia magna. The purpose of this research was to know the influence of different temperature and ephippia density for ephippia hatch of Daphnia magna. This research has experimental characteristic and use Factorial Completely Randomized Design with three experiments of temperature (A factor), three experiments of density (B factor) and three times replicated. There are 9 kinds of combination between density and temperature. The parameter which checked in this research is degree of hatch of ephippia Daphnia magna and water quality that is dissolve oxygen rate, NH3 and of pH hatch media. The result of the analysis showed that the temperature and density influence of hatching Daphnia magna ephippia. The best result can be used for hatching Daphnia magna ephippia is temperature at 25oC with density 50 ephippia /100 ml giving highest result for the hatch of ephippia Daphnia magna. There are interaction betwen temperature and density for hatching.
Pengkayaan Daphnia spp. Dengan Viterna Terhadap Kelangsungan Hidup Dan Pertumbuhan Larva Ikan Lele Dumbo (Clarias gariepinus) [Enrichment Of Daphnia spp. With Viterna To Survival And Growth Of African Catfish (Clarias gariepinus) Larvae] Boedi Setya Rahardja; Woro Hastuti Satyantini; Naila Budiatin Wahyu Mufidah
Jurnal Ilmiah Perikanan dan Kelautan Vol. 1 No. 1 (2009): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v1i1.11699

Abstract

Abstract African catfish (Clarias gariepinus) is one of fish consumption use to fulfill animal protein. The fulfillment need of animal protein with aquaculture effort, where as in the aquaculture effort will larvae quality increased. Increasingly of larvae quality with natural food had been adding feed supplement. Natural food to used is Daphnia spp. and then food supplement to used is viterna. The viterna will increase Daphnia nutritions, because of the viterna contains many nutritions to survival and growth of African catfish. The aim of this study was to know the influence of Daphnia spp. enrichment with viterna to survival and growth of African catfish (Clarias gariepinus) larvae. The rearing of larvae during 40 days. This study used Complete Random Design. The treatment were A (present Daphnia spp. without viterna), B (Daphnia spp. enrichment with viterna 10 ml/L dose), C (Daphnia spp. enrichment with viterna 10 ml/L dose), D (Daphnia spp. enrichment with viterna 10 ml/L dose), E (Daphnia spp. enrichment with viterna 10 ml/L dose) dan F (Daphnia spp. enrichment with viterna 10 ml/L dose), each treatment with 4 replications. The result show that viterna applications to Daphnia was significantly influenced (p<0,05) to survival African catfish larvae. The highest survival was E treatment (7,25 %). The viterna leaf to Daphnia spp. Was significantly influenced (p<0,05) to specific growth rate African catfish larvae. The highest specific growth rate was B treatment (1,885 %BW/day).
Co-Authors A. Shofy Mubarak A.A. Ketut Agung Cahyawan W Abdul Manan Abdul Manan Ade Wahyu Pratama Adriana Monica Sahidu Agus Nazarudin Yahya Agustono - Agustono . Agustono Agustono Agustono Agustono Agustono Agustono Ahmad Nizar Fanani Aisyah Afrianti Akbar Falah Tantri Akhmad Syafroni Affandi Akhmad Taufiq Mukti Alanosi Noor Muhammad Alma Ika Fatmawati Andry Wijayanto Angga Bahtera Siswanto Ardiany, Mega Fitria Ayu Herdianti Primashita Baskara Adam Saleh Budiana Budiana Cahyani Prasetyawati, Fatimah Chandra Bagas Swastika Bagas Cindi Koes Farizky DARMAWAN SETIA BUDI Denny Setiabudi Denta Batara Kusuma Hendra Devieta Sari Dian Viva Aurora Mayori Dwi Ernawati Ellavida Anindya Endah Sih Prihatini Endang Dewi Masithah Esti Sudaria Ulfah Faisol Mas’ud Fajar Septian Aji Fani Fariedah Farah Nabilah Farizka Vinka Trinendyah Farizky, Cindi Koes Febryan Adi Sukoco Fitria Yusmita Sari Fitriani, Mirna Friska Marsyalita Fuquh Rahmat Shaleh Gaby Stephani Rohy Gunanti Mahasri Hanif Azhara, Muhammad Hari Suprapto Hartik Hartik Ike Yunita Indra Swari Indah Permata Sari Intan Cahyarini Kamiliya Zahrah Taher Khilyatun Nisak Kurnia Ayu K. W Lailatul Lutfiyah, Lailatul Laksmi Sulmartiwi Laksmi Sumartiwi M Ervany Eshmat M. Arief Mahiastuti Tribuana Tungga Dewi Maya Kartika Eismaputeri Mega Fitria Ardiany Mirna Fitrani Mirna Fitrani Mirna Fitrani Mirna Fitrani Mirna Fitriani Mizar Desrialdi Moch Saad Mochamad Rendy Sety0 Budi Mochammad Amin Alamsjah Mohammad Faizal Ulkhaq Muhamad Amin Muhammad Arief Muhammad Arief Muhammad Nur Faith Zulkarnain Muhammad Syahrian Naila Budiatin Wahyu Mufidah Nanuk Qomariyah Nizar Fanani, Ahmad Nuning Vita Hidayati Nuning Vita Hidayati Nuning Vita Hidayati Nurita Wahyuni Ongky Wijaya Permana Sulistyo Rini Prayogo Prayogo Prayogo Prayogo Prayogo Primashita, Ayu Herdianti Pursetyo, Kustiawan tri Putri Alia Paramitha Rahayu Kusdarwati Rani Andrianti Ekaputri Rena Wilis Putri Reysa Sasmaya Wahyadyatmika Reza Nurhuda Rodhiatul Ardiani Rr. Juni Triastuti Sapto Andriyono Silvi Hardiyana Singgih Aditama Siti Nurafifah Suciyono, Suciyono Sudarno Sudarno Syahrian, Muhammad Syarifa Bintang Maharani Tri Nadya Olyvia Kerin Hardianie Vega Chrisnawati W. Angan Indrawan Wahyuni, Nurita Widy Ayunanti Wiku Bakti Bawono WINARTI WINARTI Woro Hastuti Setyantini Yudi Cahyoko Yulian Cindra Eka Pradana Yusuf Bangun Lastianto Yusuf Taufik Hidayat