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Pengaruh Perbedaan Konsentrasi Gliserol dalam Susu Skim Kuning Telur untuk Proses Penyimpanan Sperma Beku terhadap Motilitas dan Viabilitas Spermatozoa Ikan Patin (Pangasius pangasius) [Effect Of Different Glycerol Concentration In Skim Milk And Egg Yolk Diluter For Storage Process Of Frozen Semen On Sperm Motility And Viability Of Catfish (Pangasius pangasius)] Sudarno Sudarno; Bagus Rizki Novianto; Endang Dewi Masithah
Jurnal Ilmiah Perikanan dan Kelautan Vol. 6 No. 1 (2014): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v6i1.11356

Abstract

Abstract Needs of catfish seed always increase every years, especially for farming activities. Fulfillment demand of seed in large quntities and continuing are the main obstacle in the production process of catfish. This can be overcome by the process of cryopreservation. This process requires diluents and cryoprotectants to maintain the fertility of spermatozoa. Skim milk and egg yolks diluents has been used on fish because it was applied to carp and showing a good result of sperm motility and fertilization rate. Glycerol as cryoprotectant was reported effective to be used for European Catfish than the other kind cryoprotectant. This study aimed to determine the effect of different concentrations of glycerol in the diluent skim milk and egg yolk on the motility and viability of catfish (Pangasius pangasius) spermatozoa after freezing. This research method is the experiment with completely randomized design (CRD) as the experimental design. The treatment used is different glycerol concentrations, P1 (11%), P2 (13%), P3 (15%), and P4 (17%) and each of the treatment was repeated 5 times. The main parameters measured were motility (%) and viability (%) of spermatozoa. Supporting parameters include odor, color, pH, viscosity, sperm concentration and catfish (Pangasius pangasius). Analysis of the data using Analysis Of Variance (ANOVA) and to determine the best treatment performed Duncan's Multiple Range Test with 95% confidance interval. The results showed that the effect of the concentration of glycerol in the diluent skim milk and egg yolk was not significantly (P> 0.05) on the motility and viability of catfish (Pangasius pangasius) spermatozoa after freezing. Further testing needs to be done on the level of fertility of frozen sperm, as well as the growth rate of seeds produced from sperm frozen catfish (Pangasius pangasius) products
Ibm Bagi Petani Benih Udang Windu Skala Rumah Tangga (Backyard) Di Desa Kalitengah Kecamatan Tanggulangin Sidoarjo Yang Mengalami Gagal Panen Berkepanjangan Karena Serangan Penyakit [Ibm For Seed Shrimp Farmers Family Scale (Backyard) In Kalitengah Village, Tanggulangin District, Sidoarjo Region, That Harvesting Lossed To Long Times That Caused By The Diseases] Gunanti Mahasri; Sudarno Sudarno; Endang Dewi Masithah
Jurnal Ilmiah Perikanan dan Kelautan Vol. 6 No. 1 (2014): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v6i1.11378

Abstract

Abstract Demand of seed shrimp, as specially tiger shrimps is still not enough until now, it is only 5060%, more than for 5-10 years end showed decreased point. One of factors that influenced the successfully seeds shrimp hatchery is water quality that as a life media of shrimps. A bisnis  about shrimp hatchery is still have a good market, because there are a lot of tiger shrimp pond operational, more than some time demand of the  shrimp increase fluctuative on seasonal.  The aims of this this societies service activities is applicated a new shrimp hatcher technology by using immunostimulant at Putri Mandiri Group company, it aplicated in family hatcher in  Kalitengah village, Tanggulangin  District,  Region of Sidoarjo. The immunostimulant use to increase the body deffence of the shrimp larve in hatchery to the disease attacked dan invirontment during culture periode, it will be increase the harvesting.  The method using in the activity were socialitation/counseling, dempond and guiding to application of the method of shrimp hatcher by using immunostimulant in one periode. Monitoring and evaluation about this result were done in one month after the activity ending. This result showed that aplicated immunostimulant in shrimp family  hatcher Backyard) can increased the shrimp seed harvesting of Putri Mandiri Company owner, from 900.000 to 1.600.000 shrimp seeds, it is same as that the profit increased from  8.622.000,- until  15.822.000,-  Rupiahs for one periode panen for one   container 10 tonage capacity.
Potensi Senyawa Metabolit Sekunder Infusum Daun Durian (Durio zibethinus) Terhadap Kelulushidupan Ikan Nila (Oreochromis niloticus) Pada Transportasi Ikan Hidup Sistem Kering [ The Secondary Metabolites Potential Of Infusum Durian's (Durio zibethinus) Leaves Effect To Survival Rate Of Nila (Oreochromis niloticus) On The Livefish Dry Transport System] Endang Dewi Masithah; Muhammad Sholihul Abid; Prayogo Prayogo
Jurnal Ilmiah Perikanan dan Kelautan Vol. 6 No. 1 (2014): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v6i1.11386

Abstract

Abstract Nila (Oreochromis niloticus) is one of the leading commodity and each year will always be increased in both local and export markets. It's in line and will continue to grow due to the tendency of shifting market demand for commodities is of the dead form of fish (frozen) or other processed into a form of life, especially for the needs of breeders on commodity stocks. One of the latest post-harvest technologies that can be applied in the handling of live fish is a fish transport system in the dry media without use of water as a medium of transport with the use of anesthesia using plants that naturally contain active compounds that have the potential as a anesthesia drugs. This study aimed to determine the length of time different fish living on the transportation system with particular concentration infusum dried leaves of durian (Durio zibethinus) effect on the survival rate of nila (O. niloticus). Methods of this study is an experiment with three replications with different concentration intervals. It consisted of a preliminary study to determine the potential infusum durian leaves as anesthesia and primary research material as a form of media applications on the system dry. This study was conducted at the Laboratory of the Faculty of Fisheries and Marine Airlangga University. The results showed that the effective concentration of durian leaves infusum used to transport test was 4100 ppm with an average induction time is 40 minutes and recovery time is 48 minutes. Durian leaves infusum use in nila with 4100 ppm concentration able to achieve the level of survival and long transportation times in a row 100%, 0 h, 88.89%; 2 h, 22.22%; 4 h, 0%; 6 h, and 0%, 8 h on media packaging live fish without water.
Studi Perbandingan Kemampuan Nannochloropsis sp. dan Spirulina sp. sebagai Agen Bioremediasi terhadap Logam Berat Timbal (Pb) [Comparative Study Of Ability Nannochloropsis sp. And Spirulina sp. As Agent Bioremediation Of Heavy Metal Plumbum (Pb) ] Endang Dewi Masithah; Boedi Setya Rahardja; Tri Nadya Olyvia Kerin Hardianie
Jurnal Ilmiah Perikanan dan Kelautan Vol. 5 No. 2 (2013): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v5i2.11404

Abstract

Abstract Sea water is a component that interacts with the terrestrial environment, where the discharge of waste empties into the sea to the mainland. One of the most dangerous pollutants for human health is the heavy metal plumbum (Pb). Bioremediation offers a promising alternative method and the potential to reduce the concentration of heavy metals in water. Bioremediation is the application of biological processes to recover a contaminated place by using microorganisms. Biomass of algae Nannochloropsis sp. can be used as bioremediation of heavy metals because it has the ability adsorption caused the active cluster contained therein. In addition, Spirulina sp. thought to have the ability as an agent of bioremediation of heavy metal plumbum (Pb) because the proteins and polysaccharides are high. Information about uptake ability of heavy metal of plumbum (Pb) by Nannochloropsis sp. and Spirulina sp., in order to know how it compares to the ability of Nannochloropsis sp. and Spirulina sp. in absorbing the content of heavy metal plumbum (Pb). The method used in this study is the experimental method, the test T of SPSS analysis as the experimental design. Treatment given in the form of differences in the concentration of plumbum, which include, treatment A (Nannochloropsis sp. without the addition of plumbum), treatment B (Nannochloropsis sp. 0.9 ppm with plumbum concentrations), treatment C (Spirulina sp. without the addition of plumbum) and treatment D (Spirulina sp. with plumbum concentrations 0.9 ppm) of each treatment was repeated 5 times. The main parameters measured were real heavy metal plumbum (Pb) in water culture media Nannochloropsis sp. and Spirulinna sp. The results showed that Nannochloropsis sp. and Spirulina sp. able to absorb the heavy metals plumbum (Pb) so that it can be used as a bioremediation agent, where Spirulina sp. have higher ability in absorbing heavy metals plumbum (Pb) compared with Nannochloropsis sp
Studi Perbandingan Kemampuan Nannochloropsis sp. dan Spirulina sp. sebagai Agen Bioremediasi Terhadap Logam Berat Timbal (Pb) [Comparative Study Of Ability Nannochloropsis sp. And Spirulina sp. As Agent Bioremediation Of Heavy Metal Plumbum (Pb) ] Endang Dewi Masithah; Boedi Setya Rahardja; Khilyatun Nisak
Jurnal Ilmiah Perikanan dan Kelautan Vol. 5 No. 2 (2013): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v5i2.11405

Abstract

Abstract Microalgae species Nannochloropsis sp. can used as heavy metal biosorben because of it's adsorption capability that caused by the active cluster which contained in that species (Sembiring et al., 2008). Chlorella sp. is one of phytoplankton species that has the bioaccumulation ability of heavy metals and easily cultivated (Arifin, 1997). Lead (Pb) is a mineral belonging to microelements, is a heavy metal and is a potentially toxic material. Water bodies that have been polluted by compounds or ions Pb because can lead to the death of aquatic biota, the number of Lead (Pb) present in water bodies exceeding the proper concentration (Palar, 2004). One way to anticipate the increasing heavy metal pollution in the water is to bioremediation. The research using experimentally, the research design used was completely randomized design (CRD) consisting of four treatments with five replications. The concentrations of heavy metals Plumbum (Pb) used is 0 ppm and 0.9 ppm. The main parameters in this study is the ability of bioremediation Plumbum (Pb) by Nannochloropsis sp. and Chlorella sp. SPSS analytics normality test results and test the ability of T 95% in Nannochloropsis sp. and Chlorella sp. in absorbing heavy metals Plumbum (Pb) concentrations of 0 ppm and 0.9 ppm indicate that the data is normal and the results obtained were significantly different / significant. While the analysis of SPSS test T on heavy metal absorption capability comparison Plumbum (Pb) concentration of 0 ppm and 0.9 ppm by Nannochloropsis sp. and Chlorella sp. showed that the results were not significantly different / non significant. On average results obtained, Nannochloropsis sp. have a higher capacity than Chlorella sp. in the bioremediation process of heavy metals Plumbum (Pb).
Studi Bioakumulasi Logam Berat Merkuri (Hg) pada Teripang Lokal (Phyllophorus sp.) dari Pantai Timur Surabaya – Jawa Timur [Study Of Bioaccumulation Heavy Metal Mercury (Hg) On Local Sea Cucumber (Phyllophorus sp.) from Surabaya East Coast – East Java] Endang Dewi Masithah; Rr. Febrina Anggraini Putri; Muhammad Arief
Jurnal Ilmiah Perikanan dan Kelautan Vol. 5 No. 2 (2013): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v5i2.11406

Abstract

Abstract Sea cucumber is a commodity fishery that is traded international. One of the sea cucumber that has economic value is the local sea cucumber (Phyllophorus sp.) believed that contain compounds can be immunomodulatory. Phyllophorus sp. is one of the three dominant species of sea cucumbers in abundance and distribution in the Surabaya East Coast (Winarni,dkk., 2010). Coastal environmental issues that deserve the attention is the problem of heavy metal pollution in coastal waters. The highest toxicity of heavy metals for aquatic animals and humans are mercury (Hg) (Widowati dkk, 2008). The purpose of this study was to determine levels of heavy metals bioaccumulation of mercury (Hg) in the local sea cucumber (Phyllophorus sp.) From the East Coast Surabaya and to determine the heavy metal content of mercury (Hg) in the local sea cucumber (Phyllophorus sp.) has exceeded the threshold or does not. The experiment was conducted on the East Coast Surabaya and analisys of heavy metal Mercury (Hg) in Balai Besar Laboratorium Kesehatan (BBLK) Surabaya. The method used is the method of field observation and analyzed descriptively. The results showed levels of mercury bioaccumulation of heavy metals (Hg) from BCFo-w value from 454.78– 802.05 and BCFo-s from 31,42 – 111,26. This shows Phyllophorus sp. have the ability to accumulate heavy metals with low until middle accumulation level category. Mercury content of the Phyllophorus sp. obtained range from 0.031 to 0.061 ppm, sea water ranged from 0.0015 to 0.0023 ppm and sediments ranged from 0.0417 to 0.112 ppm. Mercury levels in Phyllophorus sp. and sediment is below the NAB, but the sea water has exceeded from NAB. Water quality parameters are still in normal conditions in accordance with the quality standards KMLH (2004) that temperatures 26-28 º C, pH 7-8, salinity ranged from 28-29 "°, DO value range 4-5 mg / L and brightness between 2-3 m.
Pengaruh Pemberian Pupuk Cair Limbah Ikan Lemuru (Sardinella sp.) dengan Dosis yang Berbeda Terhadap Pertumbuhan Chlorella sp. [Effect of Liquit Fertilizer Waste Sardinella Fish (Sardinella sp.) With Different Doses To The Chlorella sp. Growth] A. Shofy Mubarak; Diana Meritasari; Laksmi Sulmartiwi; Endang Dewi Masithah
Jurnal Ilmiah Perikanan dan Kelautan Vol. 4 No. 1 (2012): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v4i1.11579

Abstract

Abstract Chlorella sp. can growth in various media that contains enough nutrients, such as N, P, K and other micro-elements. Growth of Chlorella sp. requires the availability of nutrients that can be derived from the chemical decomposition and solution results or waste. Liquid fertilizer waste sardinella fish (Sardinella sp.) is wasted and without any economic value is a new breakthrough in utilizing any part in the fisheries sector so as to maximize the potential of fisheries. Utilization of this, one of which is to make Liquid fertilizer waste sardinella fish (Sardinella sp.) which serve as nutrients for the growth of Chlorella sp. with 7 treatments and repeated 3 times. The treatment used is the doses of liquid fertilizer waste sardinella fish (Sardinella sp.) differently, namely A (0,25 ml/L), B (0,5 ml/L), C (1,75 ml/L), D (1 ml/L), E (1,25 ml/L), F (1,5 ml/L), G (Walne), H (without fertilizer). The results showed that the addition of liquid fertilizer waste sardinella fish (Sardinella sp.) with different doses give a real impact (p<0,05) for growth population of Chlorella sp. The best doses of liquid fertilizer waste sardinella fish (Sardinella sp.) for the population growth rate of Chlorella sp. was 0.75 ml / L is the treatment of C with the highest population of 3500x103 cells / ml.
Pengaruh Konsentrasi Pupuk Azolla pinata Terhadap Pertumbuhan Populasi Spirulina platensis [The Effect Of Azolla pinata Fertilizer Concentration In Spirulina platensis Population Growth ] Masithah Dewi Endang; Faricha Risma Nurani; A. Shofy Mubarak
Jurnal Ilmiah Perikanan dan Kelautan Vol. 4 No. 1 (2012): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v4i1.11581

Abstract

Abstract Spirulina merupakan salah satu jenis alga yang sangat diminati oleh produsen pembenihan dan suplemen kesehatan. Hal yang dapat mendorong peningkatan produksi Spirulina adalah peningkatkan pertumbuhan, yaitu meningkatkan jumlah sel. Salah satu cara untuk meningkatkan pertumbuhan fitoplankton adalah mengontrol kandungan nutrien baik makro maupun mikro pada lingkungan budidaya. Azolla pinata memiliki berbagai unsur hara antara lain N, P, K, Ca, S, Mg, Mn, Fe, Zn, Co. Kandungan unsur kimia dalam Azolla pinata secara kualitatif dan kuantitatif dapat memenuhi kebutuhan unsur makro dan mikro pertumbuhan Spirulina platensis. Azolla pinata merupakan tanaman paku mini yang bersimbiosis dengan cyanobacteria pemfiksasi N2 yaitu Anabaena azollae. Simbiosis ini menyebabkan Azolla pinata sebagai sumber N. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh konsentrasi pupuk Azolla pinata terhadap pertumbuhan populasi Spirulina platensis dan untuk mengetahui konsentrasi optimal pupuk Azolla pinata yang dapat menghasilkan pertumbuhan tertinggi Spirulina platensis. Penelitian dilaksanakan di Fakultas Perikanan dan Kelautan Universitas Airlangga Surabaya. Rancangan penelitian yang digunakan adalah Rancangan Acak Lengkap (RAL) dilanjutkan dengan uji jarak berganda Duncan. Bahan uji yang digunakan dalam penelitian ini adalah S. platensis yang dikultur pada botol kaca 500 ml dengan lima perlakuan dan empat kali ulangan. Bahan pupuk yang digunakan adalah 500 gram Azolla pinata dan 2 liter aquades. Konsentrasi pupuk Azolla pinata yang diberikan pada perlakuan adalah A (3,5 ml), B (5,5 ml), C (7,5 ml), D (9,5 ml), E (kontrol Walne 1 ml). Parameter utama yang diamati adalah populasi, sedangkan parameter pendukung yang diamati adalah suhu, pH, dan salinitas. Hasil penelitian menunjukkan bahwa konsentrasi pupuk Azolla pinata memberikan pengaruh yang berbeda nyata terhadap pertumbuhan populasi Spirulina platensis. Penambahan pupuk Azolla pinata kedalam media kultur menggunakan konsentrasi 3,5 ml menghasilkan populasi Spirulina platensis tertinggi sebesar 1708,6 x 103 unit/ml.
Pengaruh Pemberian Bakteri Bacillus pumilus pada kotoran Sapi Sebagai Pupuk terhadap Jumlah Kandungan Klorofil Dunaliella salina [The Effect of Bacteria Bacillus pumilus In Cow Dung As Fertilizer to Total Chlorophyll Dunaliella salina] Endang Dewi Masithah; Nuansa Adharia Ningrum; Setiawati Sigit
Jurnal Ilmiah Perikanan dan Kelautan Vol. 3 No. 1 (2011): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v3i1.11622

Abstract

AbstractDunaliella salina is one type of natural food used in the hatchery business. Production stability D. salina with an abundance of nutrients can be supported. The purpose of this study was to determine the effect and how many doses of the bacteria Bacillus pumilus best fermented in cow dung as fertilizer in increasing the population of D. salina. Research conducted at the Laboratory of Education Faculty of Fisheries and Marine Fisheries, Airlangga University Surabaya. The research design used was Completely Randomized Design (CRD) is followed by Duncan test. D. salina was cultured in 250 mL glass bottle with 5 treatments (2 treatments as a control) and four replications. Culture medium used contained 10 ppm cow dung. The concentration of cow manure are given in the study, namely A (12.5% Bacillus pumilus), B (10% Bacillus pumilus), C (7.5% Bacillus pumilus), control 1 (without fermentation), control 2 (fertilizer Walne). The results showed that the addition of Bacillus pumilus on cow dung that were cultured in culture medium D. salina can increase the amount of chlorophyll content of D. salina. Addition of Bacillus pumilus by 10% in the culture medium to produce the amount of chlorophyll-a D. salina high of 0.014055 µg/mL and chlorophyll-b of 0.009657142 µg/mL on the first day. 
Pemanfaatan Isi Rumen Sapi yang Difermentasikan dengan Bakteri Bacillus pumilus terhadap Kandungan Klorofil pada Kultur Dunaliella salina [Use of Content Cow Rumen Fermented With Bacillus pumilus Bacterial Chlorophyll Content On The Culture Dunaliella salina] Endang Dewi Masithah; Nur Choiriyah; Prayogo Prayogo
Jurnal Ilmiah Perikanan dan Kelautan Vol. 3 No. 1 (2011): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v3i1.11630

Abstract

Abstract Dunaliella salina is one type of natural food that can be used in an attempt pembeniha rumen contents of cows is one of the waste obtained from the abattoir which is rich in nutrients. Synthesis of chlorophyll a and b require N and P. The purpose of this study was to determine the effect and the best dose utilization of rumen contents of cows and Bacillus pumilus in the culture of Dunaliella salina in chlorophyll content. The research design used was Completely Randomized Design (CRD) followed by Duncan multiple range test. Treatment research Dunaliella salina was cultured in 500 mL glass bottle with 5 treatments and 4 replications. Culture medium used was sea water that has salinity 30 ppt as much as 0.5 liters of rumen fluid is added 0.5 ml of solution that has been fermented with Bacillus pumilus in accordance with the treatment dose. The results showed that the use of fermenter bacteria Bacillus pumilus in cow rumen contents effect on chlorophyll content and dose fermenter rumen bacterium Bacillus pumilus in cattle that give the best chlorophyll content in Dunaliella salina culture fermentation is the dose intreatment C (7.5 % dose fermentation) reached 0.715653211aí¬molchl/cell on the first day.