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PENGARUH PERBEDAAN PROBIOTIK KOMERSIAL TERHADAP RASIO C:N DAN N:P MEDIA KULTUR BIOFLOK PADA BAK PERCOBAAN Endang Dewi Masithah; Yurika Dwi Octaviana; Abdul Manan
Journal of Aquaculture and Fish Health Vol. 5 No. 3 (2016): JAFH Vol. 5 No. 3 September 2016
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (222.172 KB) | DOI: 10.20473/jafh.v5i3.11333

Abstract

Salah satu faktor yang menyebabkan terjadinya penurunan produksi ikan budidaya adalah adanya penurunan kualitas air sebagai akibat dari banyaknya akumulasi bahan organik baik yang berasal dari limbah metabolisme dan bahan organik lainnya. Bioflok merupakan teknologi yang menjadi salah satu alternatif pemecahan masalah limbah budidaya yang paling menguntungkan. Aplikasi bakteri probiotik yang tepat dapat membantu mengurangi kandungan bahan organik di tambak dan mempertahankan tersedianya nutrisi dari hasil penguraian bahan organik. Peningkatan C:N rasio akan meningkatkan pertumbuhan bakteri heterotrof yang pada akhirnya akan mengurangi nitrogen anorganik perairan. Rasio N:P akan berpengaruh terhadap kelimpahan fitoplankton jenis tertentu. Penelitian ini dilakukan dengan tujuan untuk mengetahui pengaruh penggunaan probiotik komersial berbeda terhadap rasio N:P dan C:N media kultur bioflok pada bak percobaan. Penelitian ini menggunakan metode statistika Rancangan Acak Lengkap. Hasil penelitian menunjukkan bahwa pemberian probiotik yang berbeda memberikan pengaruh yang tidak berbeda nyata (P>0,05) terhadap rasio C:N dan N:P. Nilai rasio C:N tertinggi pada perlakuan B (3,63), dan terendah pada perlakuan A (3,13). Nilai rasio N:P tertinggi pada perlakuan A (5,68) dan terendah C (4,36), kandungan N tertinggi pada perlakuan A (1692,6 ppm) dan kandungan P tertinggi pada perlakuan B (451,8 ppm). 
The Effect of Adding Cornstarch to The Chemical Characteristics of Green Mussel Paste Flavor Mila Ayu; Endang Dewi Masithah; Eka Saputra
Journal of Marine and Coastal Science Vol. 11 No. 2 (2022): JUNE
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmcs.v11i2.36159

Abstract

Green mussels is one of Indonesia's fisheries commodities that has a high export value. The high export activity of shellfish products will have an impact on the environment due to shell waste produced. Utilization of shell shell waste is a solution to overcome environmental pollution and can be in the form of diversification of food products that can be used as raw materials for food flavorings. Making a food flavor or natural flavor requires an emulsifier. One ingredient that can be used as an emulsifier is cornstarch. The purpose of this study was to determine the effect of adding cornstarch to the chemical characteristics of the green mussel paste flavor. This study used a Completely Randomized Design (CRD) consisting of 5 treatments with cornstarch concentration of 0%, 2.5%, 5%, 7.5%, and 10% with 4 replications. Protein and fat content are the main components of making flavors. The main parameters used in this study are flavor paste with emulsifier having proximate levels (protein, water content, and fat content) and supporting parameters are yield, VRS levels, water solubility, and pH levels. The results of this study indicate that the addition of gelatin has a significant effect (P <0.05) on the chemical characteristics of protein, fat, water content, yield, water solubility, VRS, and pH. Pasta flavor with a concentration of 10% has good levels of protein, fat, water content, yield value, water solubility , and VRS.  
Antibacterial Activity of Phyllophorus sp. Methanol Crude Extract on Vibrio alginolyticus and Vibrio harveyi Sapto Andriyono; Tatak Dwi Cahyono; Endang Dewi Masithah
Journal of Marine and Coastal Science Vol. 11 No. 3 (2022): SEPTEMBER
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmcs.v11i3.37722

Abstract

Utilization of natural materials sourced from the sea is very promising. These natural ingredients can function as antibacterials that are useful in a number of fields. This study aims to explore the extract of sea cucumber Phylloporus sp. as an antibacterial agent for Vibrio alginoliticus and Vibrio harveyi. The diffusion method was carried out with three treatments of sea cucumber extract starting at doses of 500, 1000 and 1500 mg/L with three replications for each treatment. Positive control was antibiotic Chloramphenicol 100 mg/ml (0.01%) and negative control with dimethylsulfoxide (DMSO) 10%. The test results showed that sea cucumber extract was able to inhibit both types of bacteria, visible from the inhibition zone formed. In Vibrio alginoliticus bacteria, the average inhibition zone formed was 0.433 ± 0.0288. In bacteria, Vibrio harveyii was inhibited by sea cucumber extract with an average inhibition zone of 0.516 ± 0.104. Inhibition zone of sea cucumber extract Phylloporus sp. lower than Chloramphenicol which is a commercial antibacterial.
Phytoplankton and Its Relationship to White Leg Shrimp (Litopenaeus vannamei) Culture Productivity in Alasbulu, Banyuwangi Hasna Kamilia; Bambang Budi Sasmito; Endang Dewi Masithah
The Journal of Experimental Life Science Vol. 11 No. 2 (2021)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2021.011.02.03

Abstract

Shrimp culture is related to phytoplankton's existence as a primary producer and water quality. Aside from their function as natural feed, phytoplankton has a role in maintaining the stability of the pond ecosystem. It is indicated by the high abundance and diversity of phytoplankton, as well as suitable water quality to support the productivity of shrimp culture. This research aimed to determine the correlation between phytoplankton structure community, water quality parameters, and shrimp productivity. This research used a descriptive method. This research was conducted in shrimp culture intensive system Alasbulu Village, Wongsorejo District, Banyuwangi on February-March 2020. The parameters observed are diversity and abundance of phytoplankton, water quality parameters, and production performance in each pond. Based on the results, six phytoplankton classes were identified: Bacillariophyceae (10 genera), Cyanophyceae (8 genera), Chlorophyceae (5 genera), Dinophyceae (2 genera), Euglenophyceae (1 genus), and Cryptophyceae (1 genus). Chlorophyceae dominated both ponds, followed by Cyanophyceae. Diversity index values on ponds 1 and ponds 2 were 1.39 and 1.50, respectively. Productivity of both ponds were 1.8 kg.m-2 and 1.4 kg.m-2; FCR (Feed Convertion Ratio) 1.1 and 1.3; ADG (Average Daily Growth) 0.3 g.day-1 and 0.25 g.day-1. It can be concluded from this research that high density of Chlorophyceae in phytoplankton community is one of the main causes that supported shrimp cultivation. Keywords: Banyuwangi, Litopenaeus vannamei, phytoplankton, productivity. 
Analiysis of Phytoplankton Diversity on the Productivity of Vannamei Shrimp (Litopenaeus vannamei) Intensive Pond, Jatisari Village, Banyuwangi Nabilla Anggi Juliyanto; Maftuch Maftuch; Endang Dewi Masithah
The Journal of Experimental Life Science Vol. 11 No. 2 (2021)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2021.011.02.01

Abstract

Phytoplankton can be used to estimate the potential for vannamei shrimp production. It can be used as a provider of nutrient sources and has an important role in improving water quality. This study aims to analyze the phytoplankton community structure on the productivity of intensive vannamei shrimp ponds. The research was conducted in Jatisari Village, Banyuwangi, East Java, Indonesia. Carried out parameters were the calculation of density, phytoplankton diversity index, culture performance (SR, FCR, ADG), and water quality (temperature, water transparency, salinity, pH, DO, NO2, NO3, PO4, NH4). The results showed that there were six classes and 33 genera from both ponds, Chlorophyceae (10 genera), Bacillariophyceae (8 genera), Cyanophyceae (9 genera), Dinophyceae (3 genera), Euglonophyceae (1 genus), Criptophyceae (1 genus). The index value of the two ponds shows moderate diversity, H 'pond 1 is 1.76, and pond 2 is 2.02. The two plots' cultivation performance was SR 92% and 80%, FCR 1.08 and 1.13, ADG 0.31 g.day-1 and 0.35 g.day-1, respectively. The physical and chemical parameters of the research showed a good enough value for the life of vannamei shrimp and phytoplankton.Keywords: Cultivation performance, Phytoplankton, Vannamei shrimp.
Analysis of Phytoplankton Structure Community, Water Quality and Cultivation Performance in Litopenaeus vannamei Intensive Pond Located in Tembokrejo Village, Muncar, Banyuwangi Gesang Maulana Dwi Katmoko; Yenny Risjani; Endang Dewi Masithah
The Journal of Experimental Life Science Vol. 11 No. 3 (2021)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2021.011.03.01

Abstract

Phytoplankton plays an important role in Litopenaeus vannamei cultivation. It plays a role as natural feed, water quality control, and indicator of shrimp cultivation success. The community structure of phytoplankton can be influenced by organic matter concentration in ponds. On the other hand, water quality and phytoplankton community structure also influence the productivity of shrimp cultivation. The research aimed to analyze the phytoplankton structure community, cultivation performance and the water quality in one of vananmei shrimp cultivation located in Muncar District. The research used the descriptive method. The research was conducted in two shrimp intensive system ponds in Tembokrejo Village, Muncar District, Banyuwangi, on February-March 2020. Parameters observed were composition, diversity, and density of phytoplankton, water quality parameters, and production performance in each pond. Based on the results, five phytoplankton classes was found: Bacillariophyceae (12 genera), Chlorophyceae (4 genera), Cyanophyceae (8 genera), Dinophyceae (2 genera), and Euglenophyceae (1 genus). Based on the density, Cyclotella and Chlorella were dominated in both ponds. Diversity index values on ponds 1 and ponds 2 were 1.64 and 1.71, respectively. The productivity of both ponds was 10.794 kg.ha-1 and 11.698 kg.ha-1, FCR (Feed Conversion Ratio) were 0.99 and 1.18, and ADG (Average Daily Growth) on both ponds were 0.16 g.day-1. Water quality parameters in both ponds showed an optimal range for vannamei cultivation, except phosphate, which is quite high. Overall, the cultivation performance of both ponds in our research showed good results. However, cultivation performance obtained in this research was not on its best performance yet due to Infectious Myonecrosis (IMNV) infection.Keywords: Cultivation performance, Litopenaeus vannamei, muncar, phytoplankton.
Aktivitas Enzimatis Isolat Bakteri Asam Laktat dari Saluran Pencernaan Kepiting Bakau (Scylla spp.) Sebagai Kandidat Probiotik [Activity Enzymatic of Isolate Lactic Acid Bacteria from the Digestive Tract of Mud Crab (Scylla spp.) as a Candidate Probiotics] Wahju Tjahjaningsih; Endang Dewi Masithah; Heru Pramono; Pipin Suciati
Jurnal Ilmiah Perikanan dan Kelautan Vol. 8 No. 2 (2016): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v8i2.11182

Abstract

Abstrak Probiotik dapat didefinisikan sebagai mikroba hidup yang ditambahkan dalam jumlah tertentu yang mampu bertahan hidup dalam ekosistem saluran pencernaan. Enzim yang dihasilkan oleh mikroba yang diisolasi dari saluran pencernaan ikan dapat digunakan sebagai probiotik. Enzim proteolitik ekstraseluler secara alami diproduksi oleh mikroba untuk menghidrolisis polipeptida dalam media menjadi peptida dan asam amino. Bakteri asam laktat dapat menghasil enzim seperti protease, α-amilase, fitase, kitinase, lipase. Penelitian ini bertujuan untuk mendapatkan isolat bakteri asam laktat dari saluran pencernaan kepiting bakau (Scylla spp.) yang mempunyai aktivitas proteolitik. Penelitian ini menggunakan metode deskriptif berupa aktivitas enzimatis dan karakterisasi uji biokimia isolat bakteri asam laktat dari saluran pencernaan kepiting bakau (Scylla spp.). Jumlah kepiting bakau yang digunakan adalah 10 ekor. Hasil penelitian didapatkan tiga isolat bakteri asam laktat yang memiliki aktivitas proteolitik kuat, yaitu WK 28, WK 33, dan WK 53. Hasil uji biokimia isolat WK 28 termasuk ke dalam genus Pediococcus sp., isolat WK 33 termasuk ke dalam Lactobacillus sp., dan isolat WK 53 termasuk ke dalam genus Streptococcus sp. WK 28 (Pediococcus sp.) dan WK 33 (Lactobacillus sp.) mempunyai aktivitas proteolitik dan aktivitas lipolitik. Isolat WK 53 (Streptococcus sp.) mempunyai aktivitas enzimatis yaitu aktivitas proteolitik, amilolitik, dan lipolitik. Abstract Probiotics are defined as live microbes are added in a certain amount that is able to survive in the digestive tract ecosystem. Enzymes produced by microbes isolated from the digestive tract of fish can be used as probiotics. Extracellular proteolytic enzymes naturally produced by the microbes to hydrolyze a polypeptide in a media into peptides and amino acids. Lactic acid bacteria can produce enzymes such as proteases, α-amylase, phytase, chitinase, lipase. This study aims to get the lactic acid bacteria isolates from the gastrointestinal tract of mangrove crab (Scylla spp.) That have proteolytic activity. This research uses descriptive method such as enzymatic activity and biochemical characterization of isolates of lactic acid bacteria from the digestive tract of mangrove crab (Scylla spp.). Amount of mud crab used is 10 fish. The result showed three isolates of lactic acid bacteria that have a strong proteolytic activity, namely WK 28 WK 33 and WK 53. The results of biochemical tests WK 28 isolates belonging to the genus Pediococcus sp., Isolate WK 33 belonging to the Lactobacillus sp., And WK 53 isolates belonging to the genus Streptococcus sp. WK 28 (Pediococcus sp.) And WK 33 (Lactobacillus sp.) Have proteolytic activity and lipolytic activity. Isolates WK 53 (Streptococcus sp.) Have enzymatic activity is proteolytic activity, amylolytic, and lipolytic.
Aplikasi Teknologi Asap Cair dalam Pengolahan dan Pengawetan Produk Perikanan di Pulau Mandangin [ Technology Application of Liquid Smoke in Processing and Preservation of Fishery Products in The Mandangin Island] Sapto Andriyono; Wahju Thajaningsih; Agustono Agustono; Endang Dewi Masithah; Kustiawan tri Pursetyo; Annur Ahadi Abdillah; Heru Pramono
Jurnal Ilmiah Perikanan dan Kelautan Vol. 7 No. 1 (2015): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v7i1.11228

Abstract

Abstract The increase in value-added of fishery products is one of which can be done by processing. In addition to maintaining the value of the protein in the fish raw material, it would be easier if the product is stored and increase the sale value of the fish itself. The ability to perform in the fish processing in Mandangin island is still very limited. Community service activities that have been carried out by a team of the Faculty of Fisheries and Marine Universitas Airlangga was introduce smoked fish product processing with liquid smoke,because this method easier and faster so that the products can be marketed. Liquid smoke was developed because the location is very minimal in the supply of raw materials such as firewood and coconut, so the use of liquid smoke is very appropriate. The resulting refined products are expected to be a source of protein Mandangin Island community itself and is able to become an alternative source of income for the community to increase the sale value of fishery products. Mentoring process being conducted by a team from the Faculty of Fisheries and Marine Universitas Airlangga so that the products can be marketed outside Mandangin Island or even to other areas in East Java.
Pengaruh Substrat Dasar yang Berbeda pada Sistem Resirkulasi terhadap Fisiologis Teripang Lokal (Phyllophorus sp.) selama Masa Adaptasi [Effect of Different Substrates on the Recirculation System for Local Sea Cucumber (Phyllophorus sp.) Physiological During Adaptation Period ] Sri Subekti; Tegar Putra Purnayudha; Endang Dewi Masithah
Jurnal Ilmiah Perikanan dan Kelautan Vol. 6 No. 2 (2014): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v6i2.11306

Abstract

Abstract This study aims to determine the physiological changes in local sea cucumber (Phyllophorus sp.) Were adapted on a maintenance bath recirculation system with a different substrate. This study uses three treatments and three replications. The first treatment is by using sea mud substrate, a second treatment using fine gravel substrate, a third treatment was not using the base substrate. The main parameters of physiological changes observed were local sea cucumber (Phyllophorus sp.) During the period of adaptation in maintenance bath. Supporting parameters observed were mortality rate and water quality. The results showed that physiological changes occur during adaptation changes of sea cucumber body to rounded, elongated, expanded and irregular. Sea cucumbers secrete intestines and gonads in stress response to environment. Sea cucumbers are maintained on the mud substrat was average survive for five days, fine gravel substrate for three days and without substrate for less than three days. The conclusion of this research is maintained in bath of sea cucumbers with sea mud substrate showed the best results, it is characterized by the average survival time longer and the amount of sea cucumber intestines and gonads secrete less of a sea cucumber that are maintained on the basis of substrate fine gravel substrate and without substrate.
Kejadian Kelainan Vertebra Ikan Nila (Oreochromis niloticus) pada Media Penetasan Salinitas yang Berbeda [Vertebrae Malformation Tilapia Fish (Oreochromis niloticus) on Different Media Hatching Saline] Lailatul Lutfiyah; Rr Juni Triastuti; Endang Dewi Masithah; Win Darmanto
Jurnal Ilmiah Perikanan dan Kelautan Vol. 6 No. 2 (2014): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v6i2.11312

Abstract

Abstract Tilapia fish belonged to the euryhaline fish, which has wide range tolerance in salinity (freshwater euryhaline fish has salinity tolerance >8 ppt). Incidence of fish skeletal abnormalities could be the response of the environment influence such as salinity, temperature and hypoxia. This research uses five treatments : 0,5 10, 15, 20 ppt. The results showed that the highest abnormalities found on the treatment of 15 ppt and 20 ppt, while the lowest for the treatment of abnormalities of 10 ppt. The best result to be used in the brackish nila aquaculture is 10-15 ppt in salinity.