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All Journal Jurnal Akuakultur Rawa Indonesia ILMU KELAUTAN: Indonesian Journal of Marine Sciences The Journal of Experimental Life Sciences (JELS) Jurnal Kelautan : Indonesian Journal of Marine Science and Technology Majalah Ilmiah Bahari Jogja Journal of Aquaculture and Fish Health Journal of Marine and Coastal Science Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan Journal of Fisheries & Marine
Endang Dewi Masithah
Department Of Marine, Faculty Of Fisheries And Marine, Universitas Airlangga
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Medicine & Pharmacology Neuroscience Transportation

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PENGARUH PERBEDAAN PROBIOTIK KOMERSIAL TERHADAP RASIO C:N DAN N:P MEDIA KULTUR BIOFLOK PADA BAK PERCOBAAN Endang Dewi Masithah; Yurika Dwi Octaviana; Abdul Manan
Journal of Aquaculture and Fish Health Vol. 5 No. 3 (2016): JAFH Vol. 5 No. 3 September 2016
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (222.172 KB) | DOI: 10.20473/jafh.v5i3.11333

Abstract

Salah satu faktor yang menyebabkan terjadinya penurunan produksi ikan budidaya adalah adanya penurunan kualitas air sebagai akibat dari banyaknya akumulasi bahan organik baik yang berasal dari limbah metabolisme dan bahan organik lainnya. Bioflok merupakan teknologi yang menjadi salah satu alternatif pemecahan masalah limbah budidaya yang paling menguntungkan. Aplikasi bakteri probiotik yang tepat dapat membantu mengurangi kandungan bahan organik di tambak dan mempertahankan tersedianya nutrisi dari hasil penguraian bahan organik. Peningkatan C:N rasio akan meningkatkan pertumbuhan bakteri heterotrof yang pada akhirnya akan mengurangi nitrogen anorganik perairan. Rasio N:P akan berpengaruh terhadap kelimpahan fitoplankton jenis tertentu. Penelitian ini dilakukan dengan tujuan untuk mengetahui pengaruh penggunaan probiotik komersial berbeda terhadap rasio N:P dan C:N media kultur bioflok pada bak percobaan. Penelitian ini menggunakan metode statistika Rancangan Acak Lengkap. Hasil penelitian menunjukkan bahwa pemberian probiotik yang berbeda memberikan pengaruh yang tidak berbeda nyata (P>0,05) terhadap rasio C:N dan N:P. Nilai rasio C:N tertinggi pada perlakuan B (3,63), dan terendah pada perlakuan A (3,13). Nilai rasio N:P tertinggi pada perlakuan A (5,68) dan terendah C (4,36), kandungan N tertinggi pada perlakuan A (1692,6 ppm) dan kandungan P tertinggi pada perlakuan B (451,8 ppm). 
The Effect of Adding Cornstarch to The Chemical Characteristics of Green Mussel Paste Flavor Mila Ayu; Endang Dewi Masithah; Eka Saputra
Journal of Marine and Coastal Science Vol. 11 No. 2 (2022): JUNE
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmcs.v11i2.36159

Abstract

Green mussels is one of Indonesia's fisheries commodities that has a high export value. The high export activity of shellfish products will have an impact on the environment due to shell waste produced. Utilization of shell shell waste is a solution to overcome environmental pollution and can be in the form of diversification of food products that can be used as raw materials for food flavorings. Making a food flavor or natural flavor requires an emulsifier. One ingredient that can be used as an emulsifier is cornstarch. The purpose of this study was to determine the effect of adding cornstarch to the chemical characteristics of the green mussel paste flavor. This study used a Completely Randomized Design (CRD) consisting of 5 treatments with cornstarch concentration of 0%, 2.5%, 5%, 7.5%, and 10% with 4 replications. Protein and fat content are the main components of making flavors. The main parameters used in this study are flavor paste with emulsifier having proximate levels (protein, water content, and fat content) and supporting parameters are yield, VRS levels, water solubility, and pH levels. The results of this study indicate that the addition of gelatin has a significant effect (P <0.05) on the chemical characteristics of protein, fat, water content, yield, water solubility, VRS, and pH. Pasta flavor with a concentration of 10% has good levels of protein, fat, water content, yield value, water solubility , and VRS.  
Antibacterial Activity of Phyllophorus sp. Methanol Crude Extract on Vibrio alginolyticus and Vibrio harveyi Sapto Andriyono; Tatak Dwi Cahyono; Endang Dewi Masithah
Journal of Marine and Coastal Science Vol. 11 No. 3 (2022): SEPTEMBER
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmcs.v11i3.37722

Abstract

Utilization of natural materials sourced from the sea is very promising. These natural ingredients can function as antibacterials that are useful in a number of fields. This study aims to explore the extract of sea cucumber Phylloporus sp. as an antibacterial agent for Vibrio alginoliticus and Vibrio harveyi. The diffusion method was carried out with three treatments of sea cucumber extract starting at doses of 500, 1000 and 1500 mg/L with three replications for each treatment. Positive control was antibiotic Chloramphenicol 100 mg/ml (0.01%) and negative control with dimethylsulfoxide (DMSO) 10%. The test results showed that sea cucumber extract was able to inhibit both types of bacteria, visible from the inhibition zone formed. In Vibrio alginoliticus bacteria, the average inhibition zone formed was 0.433 ± 0.0288. In bacteria, Vibrio harveyii was inhibited by sea cucumber extract with an average inhibition zone of 0.516 ± 0.104. Inhibition zone of sea cucumber extract Phylloporus sp. lower than Chloramphenicol which is a commercial antibacterial.
Aktivitas Enzimatis Isolat Bakteri Asam Laktat dari Saluran Pencernaan Kepiting Bakau (Scylla spp.) Sebagai Kandidat Probiotik [Activity Enzymatic of Isolate Lactic Acid Bacteria from the Digestive Tract of Mud Crab (Scylla spp.) as a Candidate Probiotics] Wahju Tjahjaningsih; Endang Dewi Masithah; Heru Pramono; Pipin Suciati
Jurnal Ilmiah Perikanan dan Kelautan Vol. 8 No. 2 (2016): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v8i2.11182

Abstract

Abstrak Probiotik dapat didefinisikan sebagai mikroba hidup yang ditambahkan dalam jumlah tertentu yang mampu bertahan hidup dalam ekosistem saluran pencernaan. Enzim yang dihasilkan oleh mikroba yang diisolasi dari saluran pencernaan ikan dapat digunakan sebagai probiotik. Enzim proteolitik ekstraseluler secara alami diproduksi oleh mikroba untuk menghidrolisis polipeptida dalam media menjadi peptida dan asam amino. Bakteri asam laktat dapat menghasil enzim seperti protease, α-amilase, fitase, kitinase, lipase. Penelitian ini bertujuan untuk mendapatkan isolat bakteri asam laktat dari saluran pencernaan kepiting bakau (Scylla spp.) yang mempunyai aktivitas proteolitik. Penelitian ini menggunakan metode deskriptif berupa aktivitas enzimatis dan karakterisasi uji biokimia isolat bakteri asam laktat dari saluran pencernaan kepiting bakau (Scylla spp.). Jumlah kepiting bakau yang digunakan adalah 10 ekor. Hasil penelitian didapatkan tiga isolat bakteri asam laktat yang memiliki aktivitas proteolitik kuat, yaitu WK 28, WK 33, dan WK 53. Hasil uji biokimia isolat WK 28 termasuk ke dalam genus Pediococcus sp., isolat WK 33 termasuk ke dalam Lactobacillus sp., dan isolat WK 53 termasuk ke dalam genus Streptococcus sp. WK 28 (Pediococcus sp.) dan WK 33 (Lactobacillus sp.) mempunyai aktivitas proteolitik dan aktivitas lipolitik. Isolat WK 53 (Streptococcus sp.) mempunyai aktivitas enzimatis yaitu aktivitas proteolitik, amilolitik, dan lipolitik. Abstract Probiotics are defined as live microbes are added in a certain amount that is able to survive in the digestive tract ecosystem. Enzymes produced by microbes isolated from the digestive tract of fish can be used as probiotics. Extracellular proteolytic enzymes naturally produced by the microbes to hydrolyze a polypeptide in a media into peptides and amino acids. Lactic acid bacteria can produce enzymes such as proteases, α-amylase, phytase, chitinase, lipase. This study aims to get the lactic acid bacteria isolates from the gastrointestinal tract of mangrove crab (Scylla spp.) That have proteolytic activity. This research uses descriptive method such as enzymatic activity and biochemical characterization of isolates of lactic acid bacteria from the digestive tract of mangrove crab (Scylla spp.). Amount of mud crab used is 10 fish. The result showed three isolates of lactic acid bacteria that have a strong proteolytic activity, namely WK 28 WK 33 and WK 53. The results of biochemical tests WK 28 isolates belonging to the genus Pediococcus sp., Isolate WK 33 belonging to the Lactobacillus sp., And WK 53 isolates belonging to the genus Streptococcus sp. WK 28 (Pediococcus sp.) And WK 33 (Lactobacillus sp.) Have proteolytic activity and lipolytic activity. Isolates WK 53 (Streptococcus sp.) Have enzymatic activity is proteolytic activity, amylolytic, and lipolytic.
Aplikasi Teknologi Asap Cair dalam Pengolahan dan Pengawetan Produk Perikanan di Pulau Mandangin [ Technology Application of Liquid Smoke in Processing and Preservation of Fishery Products in The Mandangin Island] Sapto Andriyono; Wahju Thajaningsih; Agustono Agustono; Endang Dewi Masithah; Kustiawan tri Pursetyo; Annur Ahadi Abdillah; Heru Pramono
Jurnal Ilmiah Perikanan dan Kelautan Vol. 7 No. 1 (2015): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v7i1.11228

Abstract

Abstract The increase in value-added of fishery products is one of which can be done by processing. In addition to maintaining the value of the protein in the fish raw material, it would be easier if the product is stored and increase the sale value of the fish itself. The ability to perform in the fish processing in Mandangin island is still very limited. Community service activities that have been carried out by a team of the Faculty of Fisheries and Marine Universitas Airlangga was introduce smoked fish product processing with liquid smoke,because this method easier and faster so that the products can be marketed. Liquid smoke was developed because the location is very minimal in the supply of raw materials such as firewood and coconut, so the use of liquid smoke is very appropriate. The resulting refined products are expected to be a source of protein Mandangin Island community itself and is able to become an alternative source of income for the community to increase the sale value of fishery products. Mentoring process being conducted by a team from the Faculty of Fisheries and Marine Universitas Airlangga so that the products can be marketed outside Mandangin Island or even to other areas in East Java.
Pengaruh Substrat Dasar yang Berbeda pada Sistem Resirkulasi terhadap Fisiologis Teripang Lokal (Phyllophorus sp.) selama Masa Adaptasi [Effect of Different Substrates on the Recirculation System for Local Sea Cucumber (Phyllophorus sp.) Physiological During Adaptation Period ] Sri Subekti; Tegar Putra Purnayudha; Endang Dewi Masithah
Jurnal Ilmiah Perikanan dan Kelautan Vol. 6 No. 2 (2014): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v6i2.11306

Abstract

Abstract This study aims to determine the physiological changes in local sea cucumber (Phyllophorus sp.) Were adapted on a maintenance bath recirculation system with a different substrate. This study uses three treatments and three replications. The first treatment is by using sea mud substrate, a second treatment using fine gravel substrate, a third treatment was not using the base substrate. The main parameters of physiological changes observed were local sea cucumber (Phyllophorus sp.) During the period of adaptation in maintenance bath. Supporting parameters observed were mortality rate and water quality. The results showed that physiological changes occur during adaptation changes of sea cucumber body to rounded, elongated, expanded and irregular. Sea cucumbers secrete intestines and gonads in stress response to environment. Sea cucumbers are maintained on the mud substrat was average survive for five days, fine gravel substrate for three days and without substrate for less than three days. The conclusion of this research is maintained in bath of sea cucumbers with sea mud substrate showed the best results, it is characterized by the average survival time longer and the amount of sea cucumber intestines and gonads secrete less of a sea cucumber that are maintained on the basis of substrate fine gravel substrate and without substrate.
Kejadian Kelainan Vertebra Ikan Nila (Oreochromis niloticus) pada Media Penetasan Salinitas yang Berbeda [Vertebrae Malformation Tilapia Fish (Oreochromis niloticus) on Different Media Hatching Saline] Lailatul Lutfiyah; Rr Juni Triastuti; Endang Dewi Masithah; Win Darmanto
Jurnal Ilmiah Perikanan dan Kelautan Vol. 6 No. 2 (2014): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v6i2.11312

Abstract

Abstract Tilapia fish belonged to the euryhaline fish, which has wide range tolerance in salinity (freshwater euryhaline fish has salinity tolerance >8 ppt). Incidence of fish skeletal abnormalities could be the response of the environment influence such as salinity, temperature and hypoxia. This research uses five treatments : 0,5 10, 15, 20 ppt. The results showed that the highest abnormalities found on the treatment of 15 ppt and 20 ppt, while the lowest for the treatment of abnormalities of 10 ppt. The best result to be used in the brackish nila aquaculture is 10-15 ppt in salinity.
Pengaruh Perbedaan Konsentrasi Gliserol dalam Susu Skim Kuning Telur untuk Proses Penyimpanan Sperma Beku terhadap Motilitas dan Viabilitas Spermatozoa Ikan Patin (Pangasius pangasius) [Effect Of Different Glycerol Concentration In Skim Milk And Egg Yolk Diluter For Storage Process Of Frozen Semen On Sperm Motility And Viability Of Catfish (Pangasius pangasius)] Sudarno Sudarno; Bagus Rizki Novianto; Endang Dewi Masithah
Jurnal Ilmiah Perikanan dan Kelautan Vol. 6 No. 1 (2014): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v6i1.11356

Abstract

Abstract Needs of catfish seed always increase every years, especially for farming activities. Fulfillment demand of seed in large quntities and continuing are the main obstacle in the production process of catfish. This can be overcome by the process of cryopreservation. This process requires diluents and cryoprotectants to maintain the fertility of spermatozoa. Skim milk and egg yolks diluents has been used on fish because it was applied to carp and showing a good result of sperm motility and fertilization rate. Glycerol as cryoprotectant was reported effective to be used for European Catfish than the other kind cryoprotectant. This study aimed to determine the effect of different concentrations of glycerol in the diluent skim milk and egg yolk on the motility and viability of catfish (Pangasius pangasius) spermatozoa after freezing. This research method is the experiment with completely randomized design (CRD) as the experimental design. The treatment used is different glycerol concentrations, P1 (11%), P2 (13%), P3 (15%), and P4 (17%) and each of the treatment was repeated 5 times. The main parameters measured were motility (%) and viability (%) of spermatozoa. Supporting parameters include odor, color, pH, viscosity, sperm concentration and catfish (Pangasius pangasius). Analysis of the data using Analysis Of Variance (ANOVA) and to determine the best treatment performed Duncan's Multiple Range Test with 95% confidance interval. The results showed that the effect of the concentration of glycerol in the diluent skim milk and egg yolk was not significantly (P> 0.05) on the motility and viability of catfish (Pangasius pangasius) spermatozoa after freezing. Further testing needs to be done on the level of fertility of frozen sperm, as well as the growth rate of seeds produced from sperm frozen catfish (Pangasius pangasius) products
Ibm Bagi Petani Benih Udang Windu Skala Rumah Tangga (Backyard) Di Desa Kalitengah Kecamatan Tanggulangin Sidoarjo Yang Mengalami Gagal Panen Berkepanjangan Karena Serangan Penyakit [Ibm For Seed Shrimp Farmers Family Scale (Backyard) In Kalitengah Village, Tanggulangin District, Sidoarjo Region, That Harvesting Lossed To Long Times That Caused By The Diseases] Gunanti Mahasri; Sudarno Sudarno; Endang Dewi Masithah
Jurnal Ilmiah Perikanan dan Kelautan Vol. 6 No. 1 (2014): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v6i1.11378

Abstract

Abstract Demand of seed shrimp, as specially tiger shrimps is still not enough until now, it is only 5060%, more than for 5-10 years end showed decreased point. One of factors that influenced the successfully seeds shrimp hatchery is water quality that as a life media of shrimps. A bisnis  about shrimp hatchery is still have a good market, because there are a lot of tiger shrimp pond operational, more than some time demand of the  shrimp increase fluctuative on seasonal.  The aims of this this societies service activities is applicated a new shrimp hatcher technology by using immunostimulant at Putri Mandiri Group company, it aplicated in family hatcher in  Kalitengah village, Tanggulangin  District,  Region of Sidoarjo. The immunostimulant use to increase the body deffence of the shrimp larve in hatchery to the disease attacked dan invirontment during culture periode, it will be increase the harvesting.  The method using in the activity were socialitation/counseling, dempond and guiding to application of the method of shrimp hatcher by using immunostimulant in one periode. Monitoring and evaluation about this result were done in one month after the activity ending. This result showed that aplicated immunostimulant in shrimp family  hatcher Backyard) can increased the shrimp seed harvesting of Putri Mandiri Company owner, from 900.000 to 1.600.000 shrimp seeds, it is same as that the profit increased from  8.622.000,- until  15.822.000,-  Rupiahs for one periode panen for one   container 10 tonage capacity.
Potensi Senyawa Metabolit Sekunder Infusum Daun Durian (Durio zibethinus) Terhadap Kelulushidupan Ikan Nila (Oreochromis niloticus) Pada Transportasi Ikan Hidup Sistem Kering [ The Secondary Metabolites Potential Of Infusum Durian's (Durio zibethinus) Leaves Effect To Survival Rate Of Nila (Oreochromis niloticus) On The Livefish Dry Transport System] Endang Dewi Masithah; Muhammad Sholihul Abid; Prayogo Prayogo
Jurnal Ilmiah Perikanan dan Kelautan Vol. 6 No. 1 (2014): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v6i1.11386

Abstract

Abstract Nila (Oreochromis niloticus) is one of the leading commodity and each year will always be increased in both local and export markets. It's in line and will continue to grow due to the tendency of shifting market demand for commodities is of the dead form of fish (frozen) or other processed into a form of life, especially for the needs of breeders on commodity stocks. One of the latest post-harvest technologies that can be applied in the handling of live fish is a fish transport system in the dry media without use of water as a medium of transport with the use of anesthesia using plants that naturally contain active compounds that have the potential as a anesthesia drugs. This study aimed to determine the length of time different fish living on the transportation system with particular concentration infusum dried leaves of durian (Durio zibethinus) effect on the survival rate of nila (O. niloticus). Methods of this study is an experiment with three replications with different concentration intervals. It consisted of a preliminary study to determine the potential infusum durian leaves as anesthesia and primary research material as a form of media applications on the system dry. This study was conducted at the Laboratory of the Faculty of Fisheries and Marine Airlangga University. The results showed that the effective concentration of durian leaves infusum used to transport test was 4100 ppm with an average induction time is 40 minutes and recovery time is 48 minutes. Durian leaves infusum use in nila with 4100 ppm concentration able to achieve the level of survival and long transportation times in a row 100%, 0 h, 88.89%; 2 h, 22.22%; 4 h, 0%; 6 h, and 0%, 8 h on media packaging live fish without water.
Co-Authors A. Shofy Mubarak Abdillah, Annur Ahadi Abdul Manan Agustono - Amilaningrum, Diadara Aondohemba Samuel Nege Bagus Rizki Novianto Bambang Budi Sasmito Boedi Setya Rahardja Diana Meritasari Dwitha Nirmala Eka Saputra Eka Saputra Erni Ristyanti Faricha Risma Nurani Gabriel Zola Scriptura Gunanti Mahasri Heru Pramono Juliyanto, Nabilla Anggi Junaidi Khotib Kamilia, Hasna Katmoko, Gesang Maulana Dwi Khilyatun Nisak Lailatul Lutfiyah, Lailatul Laksmi Sulmartiwi Luthfiana Aprilianita Sari Maftuch Maftuch Maulida Rosa Umainana Mila Ayu Mizar Desrialdi Mochamad Rendy Sety0 Budi Mohammad Faizal Ulkhaq Mohd Hanafi Idris Mohd Hanafi Idris Muhammad Arief Muhammad Sholihul Abid Nova Ariesma Nuansa Adharia Ningrum Nur Choiriyah Nurita Wahyuni Pipin Suciati Prayogo Prayogo Pursetyo, Kustiawan tri Rr. Febrina Anggraini Putri Rr. Juni Triastuti Saltsa Arinda Putri Sapto Andriyono Setiawati Sigit Silvi Hardiyana Sri Subekti Suciyono, Suciyono Sudarno Sudarno Sudarno, Sudarno Sukrismiati Sukrismiati Tatak Dwi Cahyono Tegar Putra Purnayudha Tjahjaningsih, Wahju Tri Nadya Olyvia Kerin Hardianie Wahju Thajaningsih Win Darmanto Wiwie Soemarjati Yenny Risjani Yudi Cahyoko Yurika Dwi Octaviana Zein, Alfian Zhenilla Mustika Berliana