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POTENSI PHOTODINAMIK INAKTIVASI Staphylococcus aureus DAN Vibrio cholerae DENGAN ENDOGEN PHOTOSENSITIZER PADA PENYINARAN LED BIRU (430 ± 4) nm DAN MERAH (629 ± 6) nm Suryani Dyah Astuti; Djoni Izak R.; Ni’matuzahroh; M. Zainuddin; Suhariningsih
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 16 No 2 (2011): June 2011
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/291

Abstract

Photodynamic Inactivation (PDI) is bacteria inactivation method with using light and bacteria porphyrin photosensitizer.The combination of light and photosensitizer with suitable spectrum can promote photosensitization process and then cause bacteria photodamage. This research was laboratory experiment, to analyze photodamage potency of Gram positive bacteria Staphylococcus aureus and Gram negative Vibrio cholera with combination of endogen photosensitizer and LED exposure (blue LED (430 ± 4) nm and red LED (629 ± 6) nm)) on Pulse Width Modulation (PWM) 75% and time duration 30 minutes. The viability of bacteria had been counted after 48 hours incubation on temperature 37oC by using Total Plate Count (TPC). Result of this research showed that blue LED exposure (430 ± 4) nm had potency to decrease 70% Staphylococcus aureus and 50% Vibrio cholera bacteria colony forming unit. Red LED (629 ± 6) nm exposure decreased 22% dan 3% colony forming unit. So blue LED exposure had big potency to bacteria inactivate.
MRI Cervical Image Analysis with Fat Suppression Techniques Between Sequent Turbo Invers Recovery Magnitude (TIRM) and Fat Saturations (Fat Sat) on Degenerative Disc Disease at Haji Hospital Surabaya Vita Arinda Ayu Putri Nata; Suryani Dyah Astuti; R. Arif Wibowo
Indonesian Applied Physics Letters Vol. 2 No. 1 (2021): June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/iapl.v2i1.28301

Abstract

This study aimed to analyze the difference of image information and determine the better image quality between the Turbo Invers Recovery Magnitude (TIRM) and Fat Saturation (Fat Sat) techniques on cervical MRI examination at Haji Hospital Surabaya. The samples of this study were one female and three male patients. The obtained data were ROI of the vertebral body tissue, intervertebral discs, spinal cord, and then calculated as SNR and CNR values. SNR and CNR values were statistically tested using Paired T-Test on SPSS 2.5. The Paired T-Test results showed a significant difference between the TIRM and Fat Sat techniques, p>0.05. SNR and CNR average values also proved that the Fat Sat technique had more optimal image quality than the TIRM technique.
Identification of Stroke with MRI Images Using the Learning Vector Quantization (LVQ) Method Based on Texture Features Endah Purwanti; Lellen Novia Hariono; Suryani Dyah Astuti
Indonesian Applied Physics Letters Vol. 3 No. 2 (2022): December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/iapl.v3i2.40958

Abstract

Research on the Identification of Stroke with MRI Imagery Using the Learning Vector Quantization (LVQ) Method Based on Texture Features has been carried out. This study aims to determine the program's best parameters and the highest accuracy level of the stroke identification program. This research was conducted at Haji Sukolilo Hospital - Surabaya by obtaining 57 images of stroke patients and 15 images of regular patients. The study used the intelligence of stroke, tumor, and standard images to determine each category's image characteristics. After knowing the differences in each class, the next process is digital image processing, followed by feature extraction used is the Gray-Level Co-occurrence matrix (GLCM) with four parameters: contrast, correlation, diversity, and homogeneity. These four parameters are the best input parameters with an intelligence rate of 0.100 with a decrease in intelligence rate of 0.100, so the best accuracy value for training is 74.97%, and test data is 78.60%. Regarding the program's ability to correctly identify 11 data from 14 data tested, the program is feasible to be used as a second opinion.
RANCANG BANGUN PROTOTIPE 3 DIMENSI ORGAN MANDIBULA MENGGUNAKAN CITRA MEDIS RADIOLOGI Amillia Kartika Sari; Riries Rulaningtyas; Khusnul Ain; Suryani Dyah Astuti; Soegianto Soelistiono; David Buntoro Kamandjaja
Medika Respati : Jurnal Ilmiah Kesehatan Vol 17, No 4 (2022)
Publisher : Universitas Respati Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35842/mr.v17i4.762

Abstract

Latar belakang: Tumor pada mandibula dapat menyebabkan kecacatan tulang. hal ini memberikan dampak negatif pada kehidupan sosial penderita. Solusi pada kasus ini adalah operasi rekonstruksi mandibula. Untuk mengoptimalkan operasi tersebut salah satunya dapat digunakan prototipe 3D sebagai perencanaan pra-bedah. Tujuan: Penelitian ini berfokus pada proses pembuatan prototipe 3D yang menggunakan pencitraan dari modalitas CT-Scan. Hasil: Pembuatan prototipe 3D diawali dari akuisisi data citra CT-Scan yang selanjutnya dilakukan proses segmentasi citra dan visualisasi 3 dimensi, pada proses terakhir dilakukan pencetakan 3 dimensi. Prototipe 3D yang telah jadi dilakukan analisa kualitatif melalui pengukuran dimensi panjang di daerah ramus, angulus, dan body of mandible dan dibandingkan dengan hasil pengukuran organ mandibula cadaver. Didapatkan hasil rerata panjang ramus pada mandibula cadaver adalah 33,62±0,34 mm, sedangkan panjang ramus pada mandibula prototipe 3D adalah 32,98±0,44 mm. Nilai rerata pengukuran pada daerah angulus adalah 31,26±0,25 mm pada mandibula cadaver, dan nilai 31,23±0,22 mm pada mandibula protptipe 3D. Dan pengukuran pada daerah body of mandible  mandibula cadaver adalah 32,05±0,98mm, sedangkan apada mandibula prootipe adalah 32,06±1,03 mm, secara keseluruhan akurasi pada prototipe 3D sebesar 99,317%.  Kesimpulan: Penggunaan citra radiologi sebagai data awal untuk membuat prototipe 3 dimensi mandibula dapat dilakukan, pengukuran akurasi prototipe 3D harus dievaluasi untuk masing-masing tahap fabrikasi.
PENDEKATAN PENGOBATAN TRADISIONAL MELALUI ASUHAN MANDIRI PADA PENDERITA HIPERTENSI DI DESA CANGKIR KECAMATAN DRIYOREJO GRESIK Dwi setiani; Rini Hamsidi; Maya Septriana; Myrna Adianti; Ario Imandiri; Edith Frederika Puruhito; Suryani Dyah Astuti; Annisa Mayzealuna; Siska Arianti; Carissa Alfreda Assyarahil; Samy Bazher; Nidaul Fauziah; Syarahiel Hamdani; Anem Alimdam
Mosiraha: Jurnal Pengabdian Farmasi Vol. 1 No. 1 (2023): Edisi April 2023
Publisher : Fakultas Farmasi Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Hipertensi merupakan tantangan besar di Indonesia. Hipertensi merupakan peningkatan tekanan darah sistolik lebih dari 140 mmHg dan tekanan darah diastolik lebih dari 90 mmHg pada dua kali pengukuran dengan selang waktu lima menit dalam keadaan cukup istirahat/tenang. Hasil pengkajian di Wilayah desa cangkir Driyorejo ini menunjukan bahwa adanya peningkatan penyakit kronis dan tidak menular seperti hipertensi pada suatu kelompok masyarakat. Hal ini didukung oleh sebagian masyarakat berpendapat bahwa pola makan sehat dengan membuat dan mengolah sendiri sendiri dapat menyebabkan kurangnya waktu untuk bekerja menjadi buruh pabrik. Subjek dalam pengabdian masyarakat kami adalah 15 orang warga di desa Cangkir Driyorejo Gresik yang memiliki masalah kesehatan berupa hipertensi. Tempat dan lokasi pengabdian masyarakat dilaksanakan di salah satu rumah warga yang bertempatan di RT 9 RW 2 Desa Cangkir Kecamatan Driyorejo. Rangkaian kegiatan dalam pengabdian masyarakat meliputi pemberian terapi selama kurang lebih 3 bulan yang diadakan satu kali tiap minggunya pada bulan Maret hingga Juni, serta Penyuluhan mengenai asuhan mandiri melalui pendekatan pengobatan tradisional pada kasus hipertensi dan gangguan muskuloskeletal pada bulan Mei dan Juni. Hasil dari kegiatan ini diukur dengan melihat dan melakukan perbandingan data tekanan darah responden selama kurang lebih 3 bulan untuk melihat penurunan tekanan darah responden. Setelah dilakukan terapi akupuntur selama 7 kali dan pemberian teh herbal sebanyak 3 kali yang diminum selama 3 minggu oleh responden. Didapati bahwa sebelum dilakukan rangkaian terapi pada responden, rata-rata tekanan darah responden yaitu Indikator penilaian keberhasilan penyuluhan dinilai berdasarkan adanya peningkatan pengetahuan warga mengenai tekanan darah tinggi yang dilihat dari hasil pre-test dan post-test yang diberikan pada saat sebelum dan sesudah dilakukannya penyuluhan. Berdasarkan hasil kegiatan dapat disimpulkan terdapat penurunan tekanan darah pada responden setelah dilakukan terapi selama 3 bulan dan peningkatan pengetahuan mengenai hipertensi setelah dilakukan penyuluhan.
COMBINATION OF ELECTROACUPUNCTURE THERAPY WITH HERBAL ROSEMARY (ROSEMARINUS OFFICINALIS L.) IN CASE OF OBESITY Widya Retnaning Puspita; Abdul Rahman; Suhariningsih; Suryani Dyah Astuti; Nurul Fitriyah; Winarno; Katherine; Ahmad Faizin Alma; Mohammad Zulkarnaen
Jurnal Biosains Pascasarjana Vol. 25 No. 2 (2023): JURNAL BIOSAINS PASCASARJANA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbp.v25i2.2023.98-107

Abstract

Obesity or overweight is a condition that occurs when the quantity of body fat tissue compared to total body weight is greater than the normal state. Diet, exercise, psychological factors, heredity, hormones, and drug side effects are a few of the factors that might cause obesity. Gastric heat syndrome was present in the patient in this case study. The patients received electrostimulator-assisted acupuncture treatment 12 times for a total of 15 minutes at the following points: Fenglong (ST40), Neiting (ST44), Sanyinjiao (SP6), and Quchi (LI11), and was also given the herbal remedy rosemary (Rosmarinus officinalis L.) for 28 days, taken twice daily. Through the activation of PPAR- g, inhibition of prankeas lipase, and inhibition of pre-adipocyte development into adipocytes, carnosic acid, a component of rosemary, helps people lose weight. Additionally, carnosic acid stimulates the excretion of fat in feces. According to the case study results, the patient's body weight dropped from 77.6 kg to 73.9 kg. This indicates that acupuncture treatments at the points Fenglong (ST40), Neiting (ST44), Sanyinjiao (SP6), and Quchi (LI11) together with the administration of rosemary herbs can result in weight loss of up to 3.7 kg over the course of 28 days.
The Photodynamic Effect of LED-Magnetic Exposure to Photoinactivation of Aerobic Photosyntetic Bacteria Astuti, Suryani Dyah
Indonesian Journal of Tropical and Infectious Disease Vol. 5 No. 1 (2014)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (818.491 KB) | DOI: 10.20473/ijtid.v5i1.208

Abstract

All photosynthetic bacteria have a major pigment of bacteriochlorophyl and accessor pigment e.g. the carotenoids, which both have an important role in photosynthesis process. This study aim to explore the exogenous organic photosensitizer from photosyntetic bacteria for photodynamic therapy application. This study is an experimental research aiming to test the potential illumination of LED with wavelength 409, 430, 528 and 629 nm, and power optimization and time exposure LED-magnetic for optimum photo activation Rhodococcus growth. The reseach design use a factorial completely randomized design with factor of power and exposure time. The number of bacterial colonies grown measure using of Total Plate Count (TPC) methods. The result of anova test shows that irradiation treatment with LED 409 nm, 430 nm, 528 nm and 629 nm significantly affects on bacterial colony growth. LED 409 nm exposure has the greatest potential to boost the growth of bacterial colonies by 77%. LED exposure and the addition of 1.8 mT magnetic fieldincreases bacterial colony growth by 98%. Results of optimization of LED and magnetic fields show power 46 mW and a 40 minute (energy dose 110 J/cm2) optimum growth of bacterial colonies increase by 184%. So LED and magnetic illumination has potentially increased the viability of an aerob photosyntetic bacteria colonies.
APPLICATION OF NEURAL NETWORKS ON BLOOD SERUM IMAGE FOR EARLY DETECTION OF TYPHUS Purnamasari, Betty; Arisgraha, Franky; Astuti, Suryani Dyah
Indonesian Journal of Tropical and Infectious Disease Vol. 4 No. 4 (2013)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (970.71 KB) | DOI: 10.20473/ijtid.v4i4.234

Abstract

Background: Typhus is a disease caused by Salmonella typhi, Salmonella paratyphi A Salmonella parathypi B, dan Salmonella paratyphi C bacteria that attacks digestive tract and caused infection in small intestine. The common test that performed in the laboratory is widal test. The result reading of the widal test still processed manually with looking the turbidity caused by the agglutination. Aim: The research was made to decrease human error by creating a program based on artificial neural network (ANN) with learning vector quantization (LVQ) method. Method: Input of this program is image of blood serum that has reacted with widal reagen. Image procesing start with grayscaling, filtering, and thresholding. Result: Output of this program is divided into two classes, normal and typhus detected. Conclusion: From this experiment result that using 24 testing data, gives the accuracy of this program 95.833% with 1 error result from 24 testing data.
OPTIMATION OF 48 kHz ULTRASONIC WAVE DOSE FOR THE INACTIVATION OF Salmonella typhi Lestari, Dwi May; Prijo, Tri Anggono; Astuti, Suryani Dyah
Indonesian Journal of Tropical and Infectious Disease Vol. 5 No. 4 (2015)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (474.592 KB) | DOI: 10.20473/ijtid.v5i4.2006

Abstract

This study was aimed to determine the effect of ultrasonic dose exposure which could decrease the viability of Salmonella typhi by using the variation of exposure time (15, 20, 25, and 30 minutes) and volume of bacterial suspension (2, 4, 6, and 8 ml) at constant power. The sample used was Salmonella typhi. Ultrasonic wave transmitter was a piezoelectric tweeter with 0,191 watts of power and 48 kHz frequency generated by the signal generator. Piezoelectric tweeter was a kind of transducer which converted electrical energy into ultrasonic energy. This research was an experimental laboratory with a completely randomized design. The decrease of bacterial percentage was calculated by using TPC (Total Plate Count). Data were analyzed by using One Way Anova. The results showed that the variation of exposure time and volume of bacterial suspension gave significant effect on the percentage of Salmonella typhi kill. The most optimal of ultrasonic dose exposure to kill Salmonella typhi was 281.87 J/ml with 100% bacterial kill.
Electronic Nose (E-Nose) for Quality Detection of Tuna (Thunnus thynnus) Contaminated Bacteria Astuti, Suryani Dyah; Muhamad, Alfian Baggraf; Rahmatillah, Akif; Yaqubi, Ahmad Khalil; Susilo, Yunus; Aji , Angger Krisna
Indonesian Journal of Tropical and Infectious Disease Vol. 11 No. 1 (2023)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v11i1.39206

Abstract

Tuna (Thunnus thynnus) is a food that is often consumed raw to support raw food diet activities, so it has the potential to be contaminated with Salmonella typhi bacteria. Fish can be contaminated by bacteria due to their high water and protein content. Indonesia is the world's main tuna producer. Salmonella typhi detection in fresh tuna in Indonesia must be negative for Salmonella microbial contamination in order to meet food safety requirements. Microbial testing has drawbacks, such as long delays. An electronic nose was used to detect Salmonella typhi bacteria in tuna fish. The sample used consisted of 3 kinds of samples: Salmonella typhi bacteria, tuna, and tuna with Salmonella typhi contamination. The research was conducted with a shelf life of 48 hours and a sensing period every 6 hours with a sensor array of 8 sensors. The sensor output data is processed using the PCA (Principal Component Analysis) method. Through the PCA method, each variation of bacterial treatment can be classified. The result of the cumulative percentage variance of the two main components (PC) in the classification test between Salmonella typhi, tuna, and tuna with Salmonella typhi bacteria contamination was 90.5%. The most influential sensors in this study are TGS 825 for PC1 with a loading value of 0.625 and TGS 826 for PC2 with a loading value of -0.753. Therefore, it can be concluded that an electronic nose can classify between pure tuna and tuna contaminated with Salmonella typhi bacteria.
Co-Authors Abd Razak, Nasrul Anuar Abdul Rahman Abror, Ghulam Muhammad Adi Prasetyo Hutomo Agesti, Dyah Ahmad Faizin Alma Ahmad Shofy Mubarak Ahmad Taufiq Mukti Aisya, Rohadatul Aji , Angger Krisna Akhmad Muzamil Akhmad Muzamil Alma, Ahmad Faizin Ama, Fadli Amillia Kartika Sari Amruloh, Yazid Muhammad Andi Hamim Zaidan Anem Alimdam Annisa Mayzealuna Arifa Mustika Arifianto, Deni Arifianto, Deny Ario Imandiri, Ario Bambang Haris Suharmono Betty Purnamasari, Betty Carissa Alfreda Assyarahil David Buntoro Kamandjaja Destiani, Reza Djoni Izak R. Dwi May Lestari, Dwi May Dwi setiani Edith Frederika Puruhito, Edith Frederika Endah Purwanti Farhah, Ghinaa Rihadatul Aisy Franky Arisgraha, Franky Hilmaniyya Hilmaniyya Ika Yuni Anggraini Kadafi, Muhammad Syaekar Katherine Katili, Rifany Humairah Purnama Khusnul Ain Khusnul Ain Khusnul Ain Lellen Novia Hariono Luthfiyah, Sari M. Zainuddin Moh. Yasin Mohammad Zulkarnaen Muhamad, Alfian Baggraf Myrna Adianti Myrtati Dyah Artaria, Myrtati Dyah Nidaul Fauziah Ni’matuzahroh Nobuo Funabiki, Nobuo Nur Vita Indri Nur Vita Indri Astutik Nurdin, Dezy Zahrotul Istiqomah Nurul Fitriyah Nurul Fitriyah Patmadevi, Maulia Permatasari, Perwira Annissa Dyah Prihartini Widiyanti Purbandini Soeparman, Purbandini R Arif Wibowo Rahmatillah, Akif Ramadhani, Nadia Nur Rania Basalamah Reza Destiani Rini Hamsidi Riries Rulaningtyas Rohman, Muchammad Nurur Rozykulyyeva, Lale Salwa, Umaimah Mitsalia Ummi Samatha, Syifa Candiki Samian Samy Bazher Septriana, Maya Siska Arianti Siswanto Soegianto Soelistiono Suhariningsih Suhariningsih Supadi Supadi Susilo, Yunus Syahrom, Ardiyansyah Syarahiel Hamdani Tri Anggono Prijo Tri Anggono Prijo, Tri Anggono Vita Arinda Ayu Putri Nata Widya Retnaning Puspita WINARNO Winarno Winarno Winarno Winarno, Winarno Yaqubi, Ahmad Khalil Yonatan Zain, Nuril Jannah