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All Journal bionature AL KAUNIYAH BERKALA SAINSTEK Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati SEMIRATA 2015 BioWallacea Journal of Biological Research agriTECH Indonesian Journal of Biotechnology Jurnal Perlindungan Tanaman Indonesia JURNAL BIOLOGI INDONESIA BIOMA : Jurnal Biologi Makassar ALCHEMY Jurnal Penelitian Kimia Jurnal Pengabdian Pada Masyarakat SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Prosiding Seminar Nasional Riset Kuantitatif Terapan 2017 Biosaintifika: Journal of Biology & Biology Education Abdimas Umtas : Jurnal Pengabdian kepada Masyarakat Berkala Penelitian Agronomi Jurnal ABDINUS : Jurnal Pengabdian Nusantara IJOEP : International Journal of Ecophysiology Jurnal Pengabdian UNDIKMA Jurnal Biofiskim: Pendidikan dan Pembelajaran IPA Molekul: Jurnal Ilmiah Kimia Majalah Pengabdian Indonesia Jurnal Pusat Kolaborasi Pengabdian Kepada Masyarakat Bionature

Nur Arfa Yanti

Jurusan Biologi FMIPA Universitas Halu Oleo, Kendari

Author-ID : 333721

Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Nursing Physics Public Health Social Sciences Veterinary Other

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SCREENING BAKTERI AMILOLITIK DAN SELULOLITIK DARI LIMBAH SAGU (Screening of Amylolytic and Cellulolytic Bacteria From Sago waste) Yanti, Nur Arfa; Munir, Asmawati
Jurnal BioWallacea Vol 1, No 1 (2014)
Publisher : Jurnal BioWallacea

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Screening of indigenous bacteria from sago waste based on amylolytic and cellulolytic activity was done to obtain bacterial isolate having double activity, i.e. could to hydrolize of starch (amylolytic) and cellulose (cellulolytic). Screening amylolytic and cellulolytic bacteria was done based on amylolytic and cellulolytic activity on agar media. Determination of amylolytic activity on starch agar media was based on the presence of clear zone around the bacterial colony upon flooding with lugolâs iodine solution. Cellulolytic activity was determine based on the presence of clear zone around the bacterial colony on Carboxy methyl cellulose (CMC) agar upon flooding with congo red solution. Presence of a clear zone around the colony indicated starch and cellulose hydrolysis. The diameters of clear zone produced on CMC and starch agar were measured and used as an indication of the amylolytic and cellulolytic activities of the bacteria. The results of the screening based on amylolytic and cellulolytic activity showed that a number of 21 bacterial isolates that having both activities. LCA2 was the bacterial isolate with the highest amylolytic and cellulolytic activity as revealed by the size of clearing zone on both types of agar plates. The diameters of clear zone on starch and CMC agar were 4,98Â Â and 3,65 cm2, respectively. Therefore, LCA2 isolate was bacterial isolate that potent for biconvertion sago hampas into value-added products. Keywords : Bacteria, Amylolytic, Cellulolytic, Sago waste.

Analisis Kuantitatif Mikroorganisme pada Ragi Tape Lokal dan Daya Terima Tape Jusinta yang dihasilkan Nurhayani H. Muhiddin; Ramlawati Ramlawati; Nur Arfa Yanti; Abdul Mun'im
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 6, No 2 (2019): BioWallacea and Biotechnological Science
Publisher : University of Halu Oleo

The purpose of this study were to analyze the quantity of microorganisms in some local ragi tape and determine the panelists' acceptance of the "tape jusinta" was produced. This type of research is an experiment with a solid substrate fermentation method. The used ragi tape is 4 kinds of local ragi tape from South Sulawesi Province (ragi tape A, B, C, and D) and 1 type of local ragi tape from Southeast Sulawesi Province (ragi tape E). The Tape Jusinta was produced by fermentation of a solid mixture of purple sweet potato and cassava tubers in a ratio of 1: 1 using 5 kinds of local ragi tape. Analysis of microbial quantity in ragi tape includes the number of molds, yeasts and bacteria in viable count using the Standard Plate Count (SPC) method. Panelist acceptance of the Tape Jusinta product was analyzed using the Hedonic test with a numerical scale range of "6". Data were analyzed descriptively and inferentially using Anova and Duncan's Multiple Test. The results of the data analysis showed that the level of palatability of the panelists to the tapejusinta fermentedragi tape B was not different from the tape jusinta fermented ragi tape A and C, but the tape jusinta fermented by Cragi tape was different from the results of fermentation ragi tape D and E. The “tape jusinta” fermented ragi tape B is preferred over the tape jusinta fermented ragi tape A, C, D and E with the highest average panelist rating of 4.15. The quantity of microorganisms on ragi tape B are mold types as much as 1.0 x 104 cfu / g, yeast 8.0 x 103 cfu / g and bacteria 5.7 x 104 cfu/ g. Keywords: cassava roots, purple sweet potatoes, ragi tape, "tape jusinta", palatabilityAbstrakTujuan penelitian adalah menganalisis kuantitas mikroorganisme pada beberapa ragi tape lokal dan mengetahui daya terima panelis terhadap “tape jusinta” yang dihasilkan. Jenis penelitian adalah eksperimen dengan metode fermentasi substrat padat. Ragi tape yang digunakan adalah 4 macam ragi tape lokal yang berasal dari Provinsi Sulawesi Selatan (ragi tape A, B, C, dan D) dan 1 macam berasal dari Provinsi Sulawesi Tenggara (ragi tape E). Tape Jusinta dihasilkan melalui fermentasi substrat padat campuran ubi jalar ungu dan umbi ubi kayu dengan perbandingan 1 : 1 menggunakan 5 macam ragi tape lokal. Analisis kuantitas mikroba pada ragi tape meliputi jumlah kapang, khamir dan bakteri secara viable countmenggunakan metode Standard Plate Count (SPC). Daya terima panelis terhadap produk Tape Jusinta dianalisis menggunakan uji Hedonik dengan rentang skala numerik “6”.Data dianalisis secara deskriptif dan inferensial dengan Anava dan Duncan’s Multiple Test. Hasil analisis data menunjukkan bahwa tingkat kesukaan (palatability) panelis terhadap tape jusinta hasil fermentasi ragi tape B tidak berbeda dengan tape jusinta hasil fermentasi ragi tape A dan C, namun tape jusinta hasil fermentasi ragi tape C berbeda dengan hasil fermentasi ragi tape D dan E. Tape jusinta hasil fermentasi ragi tape B lebih disukai daripada tape jusinta hasil fermentasi ragi tape A, C, D dan E dengan rata-rata penilaian panelis tertinggi yaitu 4.15. Kuantitas mikroorganisme pada ragi tape B yaitu jenis kapang sebanyak 1.0 x 104 cfu/g, khamir 8.0 x 103 cfu/g dan bakteri 5.7 x 104 cfu/g .Kata kunci: Umbi ubi kayu, ubi jalar ungu, ragi tape, “tape jusinta”, daya terima

Karakterisasi Morfologi Phytophthora sp. Asal Buah Kakao Desa Olo-oloho, Kabupaten Konawe, Sulawesi Tenggara Muzuni Muzuni; Haidin Haidin; Nur Arfa Yanti
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 7, No 1 (2020): BioWallacea and Sains
Publisher : University of Halu Oleo

This study aimed to determine the morphological characteristics of Phytophthora sp. isolated from cocoa fruits from Olo-oloho Village, Konawe Regency, Southeast Sulawesi. Isolation of Phytophthora sp. carried out by the point method using V4 (Vegetable Juice Agar) media incubated at 27ºC for 24 hours. Morphological characterization of Phytophthora sp. included characterization of colony morphology and cell morphology. The results showed that the colony morphological characteristics were white colonies, cotton-like textures, the uneven edge of the colony, zoning and radial lines. The morphological characteristics of the cell had asexual spores in the form of sporangium and chlamydospores, hyphae are not aseptic, greenish-black zoospores, zoospores are round and double-flagged, and have sporangiophores. Keywords: Phytophthora sp., colony morphology, cell morphology

PENGARUH PENAMBAHAN GULA DAN NITROGEN PADA PRODUKSI NATA DE COCO Nur Arfa Yanti; Sitti Wirdhana Ahmad; Desty Tryaswaty; A. Nurhana
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 4, No 1 (2017): Biosains & Technology in Wallacea
Publisher : University of Halu Oleo

ABSTRAK Produk nata de coco merupakan makanan fungsional kaya serat yang dihasilkan oleh bakteri Acetobacter xylinum secara fermentasi menggunakan media air kelapa. Biosintesis nata de coco membutuhkan sumber karbon dan nitrogen. Penelitian ini bertujuan untuk mengetahui konsentrasi gula dan sumber nitrogen, ZA yang terbaik untuk memproduksi nata de coco. Produksi nata de coco dilakukan dengan menambahkan gula dengan perlakuan konsentrasi 2; 3,5; 5 dan 7,5% (b/v), dan ZA dengan perlakuan konsentrasi 0,2; 0,35; 0,5 dan 0,75% (b/v). Parameter yang diamati adalah ketebalan nata de coco yang diukur menggunakan jangka sorong dan rendemen nata. Hasil penelitian menunjukkan bahwa penambahan gula dan ZA pada media fermentasi berpengaruh terhadap produksi nata de coco. Konsentrasi gula 5% dan ZA 0,5% yang terbaik menghasilkan nata de coco. Kata Kunci : Nata de coco, Gula, Nitrogen, ZA. ABSTRACT Nata de coco is a functional food rich of fiber produce by Acetobacter xylinum bacteria by fermented using coconut water media. The biosynthesis of nata de coco requires a source of carbon and nitrogen. This study aims to determine is the best concentration of sugar and nitrogen source, ZA for producing nata de coco. The production of nata de coco was done by adding sugar with the treatment of concentration 2; 3.5; 5 and 7.5% (w / v), and ZA with a treatment concentration of 0.2; 0.35; 0.5 and 0.75% (w/v). The observed parameters include the thickness of nata de coco which was measured using calipers and nata yield. The results showed that the addition of sugar and ZA in the fermentation media affect the production of nata de coco. The concentration of sugar 5% and ZA 0.5% is the best to produce nata de coco . Keywords : Nata de coco, sugar, Nitrogen, ZA

ISOLASI, KARAKTERISASI, DAN UJI POTENSI BAKTERI PENGHASIL ENZIM TERMOSTABIL DARI SUMBER AIR PANAS LAINEA, SULAWESI TENGGARA Marwati Arji; Wina Rezky; Wa Ode Sitti Nurhaliza; Nur Arfa Yanti
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 5, No 1 (2018): Sains & Biodiversitas Wallacea
Publisher : University of Halu Oleo

ABSTRAK Penelitian ini bertujuan untuk memperoleh isolat bakteri yang berpotensi sebagai penghasil enzim ekstraseluler “Proliase” (Protease, Lipase, Amilase dan Selulase) dan mengetahui karakteristik isolat bakteri dari air panas Lainea. Tahapan-tahapan yang dilakukan dalam penelitian ini meliputi pengambilan sampel, isolasi bakteri, seleksi bakteri pada media spesifik serta karakterisasi bakteri potensial penghasil enzim ekstraseluler berdasarkan karakter fenotipik. Hasil penelitian ini diperoleh 36 isolat bakteri dan hanya terdapat 3 isolat bakteri yang memiliki potensi ganda dalam menghasilkan enzim ekstraseluler. Ketiga isolat bakteri memiliki bentuk sel batang, Gram positif, tidak menghasilkan endospora, bersifat motil, katalase positif, menunjukkan hasil negatif pada uji Indol, Methyl Red, Voges Proskauer, sitrat dan mampu memfermentasi sukrosa, glukosa dan mannitol, toleran terhadap konsentrasi NaCl 3-7%, pH 5-7 dan suhu 37-70oC. Ketiga isolat bakteri dari sumber air panas Lainea tersebut identik dengan genus acuan Listeria. Kata kunci : Air panas Lainea, Bakteri Proteolitik, Bakteri Lipolitik, Bakteri Amilolitik, Bakteri Selulolitik ABSTRACT This study aims to obtain bacterial isolates that have the potential as producers of "Proliase" extracellular enzymes (Protease, Lipase, Amylase and Cellulase) and determine the characteristics of bacterial isolates from Lainea hot water. The stages carried out in this study include sampling, bacterial isolation, bacterial selection in specific media and characterization of extracellular enzyme-producing potential bacteria based on phenotypic characters. The results of this study obtained 36 bacterial isolates and there were only 3 bacterial isolates which had double potential in producing extracellular enzymes. The three bacterial isolates have the form of stem cells, Gram positive, do not produce endospores, are motile, positive catalase, show negative results on the test of Indol, Methyl Red, Voges Proskauer, citrate and are able to ferment sucrose, glucose and mannitol, tolerant to NaCl 3- concentration 7%, pH 5-7 and temperature of 37-70oC. The three bacterial isolates from Lainea's hot springs are identical to Listeria's reference genus. Keywords : Lainea Hot Water, Proteolytic Bacteria, Lipolytic Bacteria, Amylolytic Bacteria, Cellulolytic Bacteria

Isolasi dan Seleksi Bakteri Pendegradasi Solar Dari Pelabuhan Penyeberangan Kendari – Wawonii, Sulawesi Tenggara M. Rajab Sutra Mijaya; Nur Arfa Yanti; Ardiansyah Ardiansyah; Nurhayani H. Muhiddin
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 6, No 2 (2019): BioWallacea and Biotechnological Science
Publisher : University of Halu Oleo

This study aimed to obtain the ability of bacteria to degrade diesel fuel. Method of this research was exploration method. Bacteria were isolated by enrichment method used SMSSe enriched diesel 2% (v/v). Selection of hidrocarbonoclastic bacteria based on the ability of to grow on agar medium solid. The bacteria in the test made in the form of suspension with Mc Farland Standard 0.5. Test of bacterial isolates were used 10% of the inoculum put in 150 mL media with different concentrations of diesel fuel were 1%, 2% and 3% and incubated on a rotary shaker at 120 rpm. Samples were taken on 1, 5, 10, 15 and 20 days to test diesel emulsion by centrifugation at a speed of 3500 rpm ±15 minutes, the comparison between the media and diesel 4:1. Growth in the amount of bacteria accounted by a Standard Plate Count method. The levels of the diesel rest calculated every sampling during incubation. The selection results of obtained diesel degrading bacteria isolates that PSI.1 PSII.1 and PSIII.2. All of bacteria have the ability to degrade diesel fuel in different treatment, the best result in lowered diesel fuel that were a concentration of 3% during 20 days of incubated at PSII.1 isolate, that have the highest ability to reduce levels of diesel up to 70,70%.Keywords: Hydrocarbonoclastic, Degradation, Diesel fuels ABSTRAK Penelitian ini bertujuan untuk mengetahui kemampuan bakteri dalam mendegradasi solar. Penelitian ini adalah penelitian eksploratif. Isolasi bakteri menggunakan metode enrichment dengan media yang digunakan yaitu SMSSe yang diperkaya solar 2% (v/v). Pemilihan bakteri hidrokarbonoklastik berdasarkan kemampuan bakteri tumbuh pada media agar padat. Inokulum bakteri dibuat dalam bentuk suspensi dengan Standar Mc Farland 0,5. Pengujian kemampuan degradasi solar dilakukan menggunakan media minimal dengan variasi konsentrasi solar 1%, 2% dan 3% dan diinkubasi pada rotary shaker. Sampel diambil pada hari ke 1, 5, 10, 15 dan 20 untuk uji emulsi solar dengan menghitung volume solar yang teremulsi. Pertumbuhan jumlah bakteri dihitung dengan metode Standard Plate Count. Kadar sisa solar dihitung setiap pengambilan sampel selama inkubasi. Hasil seleksi bakteri pendegradasi solar diperoleh tiga isolat yaitu PSI.1 PSII.1 dan PSIII.2. Ketiga isolat bakteri memiliki kemampuan mendegradasi solar pada perlakuan yang berbeda, hasil terbaik dalam menurunkan kadar solar yaitu konsentrasi 3% selama 20 hari inkubasi pada isolat PSII.1 yang memiliki kemampuan tertinggi menurunkan kadar solar hingga 70,70%. Kata kunci : Hidrokarbonoklastik, Pendegradasi, Solar

EKSPLORASI BAKTERI TERMOHALOFILIK POTENSIAL PENGHASIL L-ASPARAGINASE SEBAGAI ANTIKANKER DI SUMBER AIR PANAS WAWOLESEA Jamaluddin Jamaluddin; Alfin Alfin; Muzuni Muzuni; Nur Arfa Yanti
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 5, No 1 (2018): Sains & Biodiversitas Wallacea
Publisher : University of Halu Oleo

ABSTRAK Tujuan penelitian ini adalah untuk memperoleh isolat bakteri termohalofilik penghasil enzim L-aparaginase dari sumber air panas Wawolesea. Bakteri termohalofilik penghasil L-asparaginase asal sumber air panas Wawolesea diperoleh dengan tahapan: survey dan pengukuran parameter lingkungan sumber air panas Wawolesea; isolasi bakteri pada media NA (Nutrient Agar) dan seleksi bakteri penghasil enzim L-asparaginase pada media M-9. Hasil isolasi menunjukkan adanya 52 isolat bakteri termohalofilik dan 14 isolat diantaranya mampu menghasilkan L-asparaginase. Kata Kunci: Bakteri Termohalofilik, L-Asparaginase, Sumber Air Panas Wawolesea ABSTRACT The objective of this study was to obtain isolates of thermohalophilic bacteria producing L-asparaginase enzyme from Wawolesea hot spring. L-asparaginase producing thermohalophilic bacteria from the Wawolesea hot spring are obtained by steps; survey and environmental parameters measurement of Wawolesea hot spring, isolation of bacteria on NA (Nutrient agar) medium and selection of L-asparaginase producing bacteria on M-9 medium. The isolation results showed 52 isolates of thermohalophilic bacteria and 14 isolates of them capable of producing L-asparaginase. Keywords: Thermohalophilic bacteria, L-asparaginase, Wawolesea hot spring

Co-Authors A. Nurhana Abdul Mun'im Adi Parman Rudia, La Ode Ahmad, Sitti Wirdhana Alfin Alfin Amirullah -, Amirullah Andi Septiana Ardiansyah Ardiansyah Ardiansyah Ardiansyah Ardiansyah ARDIANSYAH ARDIANSYAH Ardiansyah Ardiansyah Asmawati Munir, Asmawati Asrul Sani Aswan, Muhammad Aswan Budiman, Herdi Cahyanti, Kartika Dwi Dedy Oetama Desty Tryaswaty DIRVAMENA BOER, DIRVAMENA Erni Martani Fahyuddin Fahyuddin, Fahyuddin Fatimah Alwi Albakar Haidin Haidin Haji Muhiddin, Nurhayani Handayani Hamid, Fitri Ida Usman Ida Usman Indrawati Indrawati Irnawati Irnawati Jamaluddin Jamaluddin Jamaluddin Jamaluddin Jamili Jamili Karya, Adi Kurnia Yati La Ode Adi Parman Rudia La Ode Adi Parman Rudia La Ode Ahmad Nur Ramadhan La Ode Iman Sulaiman La Ode Kadidae, La Ode Langkah Sembiring Lili Darlian, Lili M. Jahiding M. Rajab Sutra Mijaya Marlina, Wa Ode Leni Marwati Arji Mashuni Mashuni Mashuni Pallawagau Muhammad Jahiding Muhiddin, Nurhayani Haji Muhsin Muhsin Muhsin Muhsin Muhsin Muhsin Muksar Muksar Muzuni, Muzuni Nurhayani H. Muhiddin Nurhayani H. Muhiddin, Nurhayani H. Nurhayani Haji Muhiddin Prasetya, Wandy Murti Ramlawati, Ramlawati Santi Septiana, Santi Sebastian Margino Sebastian Margino Sitti Wirdhana Ahmad Sitti Wirdhana Ahmad Sri Ambardini Sri Ambardini Sri Noegrohati Sulaiman, La Ode Iman Suleman, Darwis Surachma, Wahyuni Nurul Suriana Suriana Suriana Suriana Suriana Suriana Syah, Muhamad Azwar Taufik Walhidayah Vidya Nur Riska Parakkasi Wa Ode Isra Wa Ode Nanang Trisna Dewi Wa Ode Sitti Nurhaliza Wahyu Ahwarul Asis Wina Rezky

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