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Journal : Microbiology Indonesia

Genotype of Hepatitis B Virus Coinfection in Typhoid Patients SUPIANA DIAN NURTJAHYANI; RETNO HANDAJANI
Microbiology Indonesia Vol. 9 No. 3 (2015): September 2015
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (79.254 KB) | DOI: 10.5454/mi.9.3.6

Abstract

Typhoid fever can cause liver disorder and may result in complication. Studies revealed hepatic involvement in thypoid known as typhoid hepatitis. Our previous paper reported the existance of hepatitis B virus (HBV) coinfection in the serum of patients with typhoid using nested-polymerase chain reaction (PCR). Based on sequence divergence, HBV has been classified into 10 genotypes (A-J), which in prediction response and correlates with clinical outcome of chronic HBV infection. This study was conducted to determine the genotypes of HBV in typhoid patients coinfected with HBV in Tuban. Sera obtained from 5 typhoid patients positive HBV by nested PCR). Study was performed by direct sequencing BigDye V1.1 Terminator Cycle Sequencing kit and ABI Prism 310 Genetic Analyzer. Analys had been using the Genetix version 10 software to create the phylogenetic tree. Phylogenetic analysis showed 3 samples as genotype B and 2 two samples as genotype C.
Prevalence of Hepatitis B Virus Infection in Blood Donors Based on Titer Hepatitis B Surface Antigen Examination (HBsAg) SUPIANA DIAN NURTJAHYANI; RETNO HANDAJANI
Microbiology Indonesia Vol. 13 No. 4 (2019): December 2019
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (449.189 KB) | DOI: 10.5454/mi.13.4.5

Abstract

Hepatitis B remains a global public health problem. Infection from hepatitis B virus (HBV) can be transmitted through a blood test or a blood transfusion. This study was conducted to identify the prevalence of HBV infection in blood donors based on examination of HBsAg titers . Blood donors from Tuban Red Cross used as sample. The method used in this research is HBsAg titers examination performed by ELISA according to the procedureoutlined in the Kit. HBsAg titers positive mostly found in men. In men from 13 samples (8.67%) are HBsAg titers positive of 150 samples while in woman all negative for HBsAg titers from 137 samples. The average titer positive was 3.095 with a standard deviation of 0.187. While HBsAg titers negative have average of 0.03 with a standard deviation of 0.14. This study showed that the prevalence of HBV infection in blood donors is most numerous in men with HBsAg titers positive number of 8.67%.
Utilization of Leaves in Mine Reclamation Land as Organic Fertilizer with Effective Bioactivatory of Microorganism 4 (em4) and Molasses Supiana Dian Nurtjahyani; Dwi Oktafitria; Sriwulan Sriwulan; Nova Maulidina; Imas Cintamulya; Eko Purnomo
Microbiology Indonesia Vol. 14 No. 2 (2020): June 2020
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (494.467 KB) | DOI: 10.5454/mi.14.2.5

Abstract

Organic fertilizer as an alternative to reduce the scarcity of chemical fertilizers, otherwise it can improve soil fertility. PT. Semen Gresik - Semen Indonesia Persero (Tbk.) Has a lot of plants in the treatment period in ex-mining land that needs fertilizer to help plant growth. In addition, in post-mining land, there is a lot of organic waste in the form of dry leaves under the stands of reclamation trees. The organic waste has the potential to be processed and used as organic fertilizer. This study aims to determine the utilize leaf litter in the reclaimed mine land as organic fertilizer with EM 4 and Molasses bioactivators. This study used is a laboratory experimental method. The results showed the composition of solid material in the form of leaf litter, bran, cow dung and husk charcoal in a ratio of 2: 1: 1: 1 as well as looking material in the form of molasses and EM4 with a dose of 100ml per 100kg of solid material dissolved in enough water (fertilizer can be clenched and expanded slowly and not broken). Conclusion leaf litter in the former reclaimed land can be processed and used as organic fertilizer.