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Hubungan antara Tingkat Pengetahuan dengan Pola Perilaku Penanganan Sindrom Premenstruasi di Fakultas Kedokteran Universitas Sebelas Maret Perdana, David; Danudjo, Tedjo; Wasita, Brian
Nexus Kedokteran Komunitas Vol 1, No 2 (2012): Nexus Kedokteran Komunitas
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Abstract

Background: Premenstrual syndrome is an increased tension and sense of  the complaints that usually began one week to several days before menstruation. Premenstrual syndrome affects 40% women with 5-10% of them were going to be powerless. Many opinions showed that premenstrual syndrome is  an usual case, but they almost have a mistake how to handle it. This study aimed to research the relationship between the knowledge level of Premenstrual Syndrome with behavioral patterns of Premenstrual Syndrome handling. Methods: This was an analitic-observasional cross sectional study. The population was medical student’s 2011 Sebelas Maret University. A sample of 125 people was selected using total sampling. The research’s instrument used questionnaire about knowledge level of premenstrual syndrome and behavioral patterns of premenstrual syndrome handling. The questionnaire were pre-tested for its validity and reliability. The data were analized by use of Spearman, on SPSS 17.0. Results: Spearman analysis showed a statistically significant relationship between knowledge level and behavioral patterns of premenstrual syndrome handling (P = 0.002). Knowledge level of premenstrual syndrome was weak positive correlation with behavioral pattern of premenstrual syndrome handling (r = 0.272). Conclusions: There were weak positive and statistically significant relationship between knowledge level of premenstrual syndrome with behavioral patterns of premenstrual syndrome handling in Medical Student’s 2011 Sebelas Maret University. Keywords: Knowledge Level, Behavioral Patterns Handling, Premenstrual Syndrome 
Pengaruh Usia terhadap Cervical Intraepithelial Neoplasia (CIN) pada Wanita dengan Infeksi Saluran Reproduksi Berbasis Pemeriksaan Papsmear Anggraini, Dewi Okta; Dirgahayu, Paramasari; Wasita, Brian
Nexus Kedokteran Klinik Vol 2, No 1 (2013): Nexus Kedokteran Klinik
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Abstract

Background: Cervical Intraepithelial Neoplasia (CIN) is a dysplasia of cervical squamous cells posibbly turn into cervical cancer if not treated immediately. The established cause of CIN is Human Papillomavirus (HPV). Several studies showed correlation between Reproductive Tract Infection (RTI) with CIN. But, it is still controversial. Age is one of predicted factor which can influence CIN. Through some mechanisms, age can change the cervical cells. Methods: This study was cross-sectional approach. Samples were taken from medical record, papsmear’s form result and papsmear preparation in the Pathology Anatomy Laboratory, Faculty of Medicine, Sebelas Maret University from 2009-2012. Total of 410 samples were divided into two groups: control and case group. Control group contain sample without RTI while case group contain sample with RTI. Each group consisted of 205 women who subsequently divided into two subgroup, aged <40 years and ≥ 40 years. In this study, HPV infection is uncontrolled. Data were taken including name, age, clinical symptoms, ISR and CIN. These data are analyzed using Fisher test from SPSS version 19 for windows. Results: Total CIN were found: 5 samples (<40 years) and 3 samples (≥40 years) from positive RTI; 20 samples (<40 years) and 53 samples (≥40 years) from negative RTI. Fisher test results showed that age has correlation with CIN (p=0,001) and there is a significant difference risk between the age distribution with CIN [RD= -12.3%, 95%CI=(-19.8)-(-4798)]. In the negative RTI, there is a significant risk sample aged <40 years and aged ≥40 years to get CIN [RD= -21.28%, 95% CI= (-33.91)- (-8.655)]. RTI does not have correlation with CIN (p = 0.06) Conclusion: Without HPV controlled, age distribution (<40 years and ≥40 years) has an influence to the occurence of CIN and there is no correlation between RTI with CIN. Key words: Age, Cervical Intraepithelial Neoplasia (CIN), Reproduction Tract Infection (RTI)
Pengaruh Ekstrak Buah Mengkudu (Morinda citrifolia) terhadap Waktu Kematian Cacing Ascaris suum, Goeze In Vitro Deviana, Riza; Utari, Cr. Siti; Wasita, Brian
Nexus Biomedika Vol 1, No 2 (2012): Nexus Biomedika
Publisher : Nexus Biomedika

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Abstract

Background: Mengkudu (Morinda citrifolia) has a terapeutic effect, such as antibacterial, anthelmintic and imunomodulator. This research was performed to understand the effect of Mengkudu (Morinda citrifolia) fruit extract on death time of  Ascaris suum, Goeze In Vitro. Methods: This research was performed using experimental laboratory method with post-test only controlled group design. Adult Ascaris suum, Goeze (108 worms) were divided into 9 groups. The worms treated with NaCl 0.9 % solution and Piperazine 0.4% g/ml served as negative control and positive control group consecutively. The worms treated with increasing dose of  Mengkudu fruits (Morinda citrifolia) extract (1% g/ml, 2% g/ml, 4% g/ml, 6% g/ml, 8% g/ml, 10% g/ml, and 12% g/ml) served as treatment groups. Observations were performed every half hour until the worms died. LC50 and LT50 were calculated using probit analysis. Statistic analysis was performed using Kruskal-Wallis test continued by Post hoc Test with Bonferroni correction. Results: Probit analysis showed that LC50 and LT50 of Mengkudu fruits extract were 4.94% g/ml and 703.32 minutes. Kruskall-Wallis test showed that significance difference at least in the two groups. Post Hoc Mann-Whitney with Bonferroni correction test showed that treatment had significant difference (p < 0.001) to negative control and 12% g/ml group had significant difference to positive control group. Conclusions: Mengkudu (Morinda citrifolia) fruits extract can induce Ascaris suum death with the death time faster than standard medicine. Keywords: Mengkudu (Morinda citrifolia) extract, Ascaris suum, Piperazin. 
Pengaruh Ekstrak Nanas (Ananas Comosus (L) Merr) SEBAGAI Antihelmintik Terhadap Waktu Kematian Cacing Ascaris suum, Goeze In vitro Dewi, Cindikya Saftiari; SitiUtari, Cr.; Wasita, Brian
Nexus Biomedika Vol 2, No 1 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

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Background:Pineapple (Ananas comosus (L) Merr) was reported to have a bromelain enzyme that belonged to a group of proteolytic enzymes. This enzyme has been known as anti-edematous, anti-inflammatory, anti-thrombotic and fibrinolytic activities. The proteolytic effect of bromelain enzyme can change protein to be simple amino acid. This research was performed to understand the effect of pineapple (Ananas comosus (L) Merr) extract as anthelmintic on death time of Ascaris suum, Goeze In Vitro. Methods:This research was performed using experimental laboratory method with the post test only controlled group design. Eighty four adult Ascaris suum, Goeze were used. The worms divided into seven groups consisted of six treatment groups (pineapple (Ananas comosus (L) Merr) extract with the dose of 0 % g/ml, 3 % g/ml, 6 % g/ml, 12 % g/ml, 24 % g/ml and 48 % g/ml) and positive control group (Pirantel pamoat with the dose of 0.236% g/ml). Each group consisted of four worms. Ascaris suum, Goeze were incubated in incubator at 37ºC. Observation was performed every fifteen minute to determine the death time of the worms. Experimental data was analyzed using One Way ANOVA test continued with Post hoc Tukey test (α = 0.05). LC50 and LT50 were calculated using probit analysis.   Results:One Way ANOVA test showed significance difference (p < 0.05). Pineapple (Ananas comosus (L) Merr) extract had a significant effect on the worm death time and showed dose dependent manner. Probit analysis showed that LC50 and LT50 of pineapple (Ananas comosus (L) Merr) extract were 21.059% g/ml and 396.929 minutes. At the concentration of 4% g/ml pineapple extract had a better effect than positive control. Conclusions:Pineapple (Ananas comosus (L) Merr) extract had an effect on the death time of Ascaris suum, Goeze which was showed by significance difference on the death time between treatment and negative control group, and this effect was comparable with positive control group. Keywords:Pineapple (Ananas comosus (L) Merr) extract, bromelain enzyme, Ascaris suum, Goeze, pirantel pamoat
Pengaruh Pemberian Topikal Ekstrak Daun Binahong (Anredera cordifolia (Ten.) Steenis) Terhadap Jumlah Sel Fibroblas Pada Penyembuhan Luka Sayat Pada Kulit Tikus Putih (Rattus norvegicus) strain wistar Aulia, Dentiko Wasis; Wasita, Brian; Pesik, Riza Novierta
Nexus Kedokteran Translasional Vol 2, No 1 (2013): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Abstract

Background: Binahong (Anredera cordifolia (Ten.) Steenis) is a common plant in Indonesia and contain many secondary metabolites such as flavonoids, tannins, saponins, terpenoids, and alkaloids. These secondary metabolite substances were expected to increase the number of fibroblast cells in the wound healing process. This study aims to demonstrate that topical administration of binahong leaf extract jelly on Wistar rats (Rattus norvegicus) skin cut will increase the number of fibroblasts in these wound healing process.   Methods: This is a laboratoric experimental study with randomize post test only control group design. The samples were 25 male Wistar rats aged 3 months and given a dorsal incision of 2 cm long and 0.5 cm deep. The rats were divided randomly into 5 groups (positive control, negative control, and 3 treatment group) consisting of 5 rats each. Positive control group were topically treated with patent medicine Bioplacenton®. Negative control group was not given any treatment. Treatment groups were topically treated with a binahong leaf extract jelly 2.5%, 5%, and 10% respectively. Bioplacenton® and binahong leaf extract jelly is given 2 times a day on its wound for 5 days. On the fifth day, all rat’s wound were scored using Bates-Jensen Wound Assassment Tool and then sacrificed to make histophatology slides on the part of the injured skin. The slides were stained with Van Giesson staining and the fibroblast in the granulation tissue was calculated. The data obtained was analyzed with Oneway ANOVA (p <0.05).   Result: The macroscopic wound scoring and average number of fibroblast cells in each group showed no significant difference (p>0.05). However, in the 2nd treatment groups (binahong leaf extract jelly 5%) showed the highest number of fibroblasts (153.72 (±SD 23.39)) than the other groups. Conclusion: Topical administration of binahong leaf extract (Anredera cordifolia (Ten.) Steenis) did not affect the macroscopic wound scoring but it increase the number of fibroblast cells in healing skin cuts on Wistar rats (Rattus norvegicus) even thought it did not reach the significant level. Keywords: wound healing, fibroblast, binahong (Anredera cordifolia (Ten.) Steenis)
Faktor sosio ekonomi demografi terhadap kejadian infeksi kecacingan pada anak Sekolah Dasar Mahmudah, Umi; Dirgahayu, Paramasari; Wasita, Brian
Ilmu Gizi Indonesia Vol 1, No 1 (2017): Agustus
Publisher : Program Studi S-1 Ilmu Gizi

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Latar belakang: Infeksi kecacingan merupakan masalah kesehatan masyarakat di negara-negara berkembang seperti Indonesia. Anak sekolah dasar merupakan golongan yang paling berisiko terhadap kejadian infeksi kecacingan. Infeksi ini dipengaruhi oleh beberapa faktor yaitu kondisi sanitasi lingkungan, perilaku personal hygiene,  serta kondisi  sosio  ekonomi demograf daerah sekitar. Kondisi sosio demograf yang berbeda di setiap wilayah mengakibatkan terjadinya infeksi kecacingan yang berbeda–beda. Tujuan: Untuk mengetahui hubungan faktor sosio ekonomi demograf  terhadap  kejadian  infeksi  kecacingan  pada  anak  sekolah  dasar. Metode: Penelitian ini merupakan penelitian observasional dengan rancangan cross sectional, pada populasi anak sekolah dasar kelas 1 sampai kelas 5 dengan jumlah 92 siswa, sedangkan besar sampel penelitian berjumlah 74 siswa di SD Barengan Kecamatan Teras Kabupaten Boyolali dengan teknik pengambilan sampel secara purposive. Data kecacingan pada anak diperoleh dengan pengambilan sampel feses dan diperiksa menggunakan metode  formol ether concentration di Laboratorium Parasitologi  Fakultas  Kedokteran  Universitas  Sebelas Maret.  Data  sosio  ekonomi  demograf diperoleh dengan kuisioner dengan metode wawancara. Analisis data diolah menggunakan chi square. Hasil: Prevalensi kejadian infeksi kecacingan pada anak sekolah dasar di SD Barengan Kecamatan Teras Kabupaten Boyolali adalah 40,21%. Pendidikan ayah (p=0,159) dan pendidikan ibu (p=0,352) tidak berhubungan dengan kejadian infeksi kecacingan. Penghasilan ayah (p=0,330) dan penghasilan ibu (p=1,152) tidak berhubungan dengan kejadian infeksi kecacingan. Kesimpulan:  Pendidikan ayah, pendidikan ibu, penghasilan ayah dan penghasilan ibu tidak berhubungan dengan kejadian infeksi kecacingan.Kata kunci: Sosio; ekonomi; demograf; infeksi kecacingan
Hubungan Kecerdasan Emosional dan Status Anemia terhadap Prestasi Belajar Mahasiswa Tingkat III Akademi Keperawatan Dharma Husada di Kediri Santoso, Puguh; Syamsulhadi, Muchamad; Wasita, Brian
Jurnal Gizi dan Kesehatan Vol 1, No 1 (2014)
Publisher : Program Studi Ilmu Gizi Pascasarjana Universitas Sebelas Maret

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Abstract

Latar Belakang : Pencapaian tujuan pendidikan tergantung pada proses belajar yang dialami individu. Salah satu faktor internal yang memiliki hubungan dengan prestasi belajar adalah kecerdasan emosi, kondisi fisik yang mengganggu kemampuan belajar, dan anemia. Tujuan untuk menganalisis hubungan kecerdasan emosional dan status anemia terhadap prestasi belajar mahasiswa Tingkat III Akademi Keperawatan Dharma Husada di Kediri.Metode Penelitian : Penelitian ini merupakan penelitian observasional dengan menggunakan rancangan cross sectional. Populasi dalam penelitian ini adalah mahasiswa Tingkat III Tahun 2013/2014 Akademi Keperawatan Dharma Husada di Kediri yang terdiri dari 2 kelas. Melalui teknik Simple Random Sampling diperoleh subjek berjumlah 91 orang. Metode untuk memperoleh datak ecerdasan emosional dengan kuesioner, kadar Hb menggunakan cyanmethemoglobin dan prestasi belajar menggunakan studi dokumentasi. Data di analisis dengan uji bivariat menggunakan regresi linier sederhana, serta multivariat menggunakan regresi linier berganda dengan tingkat kepercayaan 95% (α = 5%).Hasil : Kecerdasan emosional mempunyai hubungan yang signifikan terhadap prestasi belajar mahasiswa Tingkat III  Akademi Keperawatan Dharma Husada di Kediri (p = 0,000). Status anemia tidak mempunyai hubungan yang signifikan terhadap prestasi belajar mahasiswa Tingkat III  Akademi Keperawatan Dharma Husada di Kediri (p = 0,104). Ada hubungan yang signifikan antara kecerdasan emosional dan status anemia secara bersama - sama terhadap prestasi belajar mahasiswa Tingkat III  Akademi Keperawatan Dharma Husada di Kediri (p = 0,000). Kesimpulan : Ada hubungan yang signifikan antara kecerdasan emosional dan status anemia secara bersama – sama terhadap prestasi belajar mahasiswa Tingkat III  Akademi Keperawatan Dharma Husada di Kediri. Sumbangan kecerdasan emosional dan status anemia terhadap prestasi belajar adalah 20%, dengan kontribusi kecerdasan emosional sebesar 17,1% dan status anemia sebesar 2,9%, sedangkan 80%  berhubungan dengan  faktor lain yang tidak dianalisis dalam penelitian ini. Kata Kunci :  Kecerdasan Emosional, Status Anemia,  Prestasi Belajar
PENGARUH PEMBERIAN JUS BUAH NAGA MERAH (Hylocereus polyrhizus) TERHADAP KADAR KOLESTEROL TOTAL TIKUS PUTIH (Rattus norvegicus) JANTAN MODEL HIPERKOLESTEROLEMIA Suryani, Irma; Dirgahayu, Paramasari; Wasita, Brian
Jurnal Gizi dan Kesehatan Vol 1, No 2 (2014)
Publisher : Program Studi Ilmu Gizi Pascasarjana Universitas Sebelas Maret

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Abstract Background : Hyperlipidemia is a major cause of cardiovascular disease because it can lead to narrow of the arteries. Prevalence of diseases caused by hyperlipidemia is still quite high in many countries. Some therapeutic agents of modern medicine has been available, but most of these drug give side effects and the cost is quite high. The content of the red dragon fruit has been studied, which consists of fiber and antioxidants.This study was conducted to determine the effectiveness of antihiperlipidemia red dragon fruit juice compared with atorvastatin.Methods : Experimental with pre and post group randomized controlled design. The 36 samples were divided into six groups randomly: negative control group, positive control group (induction high fat diet for 14 days), group 1(induction high fat diet for 14 days and atorvastatin 10 days), 2, 3 and 4 (induction high fat diet for 14 days and red dragon fruit juice with a variety of doses for 10 days). The levels of lipid profiles of samples were measured before and after the intervention is given. Data were analyzed with SPSS 22.0 for windows. Result : After 14 days of margarine induction, group KP, P1, P2, P3, and P4 showed significant increase (p <0,05) on the average of total cholesterol, as well as in P1 with 0,18 mg of atorvastatin. Conclusion Red dragon fruit juice has the potential antihiperlipidemia comparable to atorvastatin Key words: Red Dragon Fruit, Lipid Profile, Atorvastatin
Sensitivity and specificity of cell block method of transthoracic fine needle aspiration in diagnosis of lung cancer Sutanto, Yusup Subagio; Santi, Nur; Wasita, Brian; Rima, Ana; Kurniawan, Hendra
Universa Medicina Vol. 40 No. 2 (2021)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2021.v40.133-140

Abstract

BackgroundLung cancer is still the main cause of cancer deaths. The high lung cancer mortality rate is caused by a diagnosis factor or therapy selection. The cell block cytology technique using fine needle aspiration (FNA) samples can provide immunocytochemical material that plays an important role in the differential diagnosis of lung cancer subtypes and in determining immunotherapy administration. This study aimed to determine the sensitivity and specificity of transthoracic FNA (TTFNA) cell block cytology in comparison with bronchial washing smears and TTFNA smears in diagnosing lung cancer. MethodsThis was a cross-sectional diagnostic study involving 26 subjects. All subjects had undergone bronchial washing and CT scan-guided fine needle aspiration followed by cell block preparation. Both direct FNA smears and cell blocks are useful in the diagnostic work-up of patients. Comparative statistical analysis of TTFNA cell block versus bronchial washing smear and TTFNA smear cytology was carried out using the McNemar test. ResultsLung cancer was found in 15 patients (57.7%) using the TTFNA cell block technique. The sensitivity and specificity of the TTFNA cell block technique were 85.7% and 75%, respectively. There was no difference in the positivity value between TTFNA cell block technique of bronchial wash smear technique, and TTFNA smear on lung cancer diagnosis (p>0.05). ConclusionsTransthoracic fine-needle aspiration in combination with the cell block technique has good sensitivity and specificity. The TTFNA can be used for immunocytochemical examinations in lung cancer diagnosis and therapy. This approach is valuable for providing individualized treatment and prognostic evaluations.
Survival and prognostic factors in pediatric osteosarcoma: A 5-year single-center experience in Central Java, Indonesia Riza, Muhammad; Salimo, Harsono; Wasita, Brian; Idulhaq, Mujaddid; Saputra, Rhyan Darma; Widyaningsih, Vitri; Soestrisno, Soestrisno; Zahidah, Fairuz; Wayanshakty, Janur
Paediatrica Indonesiana Vol 64 No 1 (2024): January 2024
Publisher : Indonesian Pediatric Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14238/pi64.1.2024.10-6

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Background The advent of chemotherapy with multimodal treatment has improved the survival rate of osteosarcoma. However, the survival rate of patients with osteosarcoma in low- and middle-income countries (LMICs) remains lower than in high-income countries (HICs). Objective To assess prognostic factors associated with survival rate of pediatric patients with osteosarcoma in Moewardi Hospital, Surakarta. Methods This retrospective study was performed in pediatric osteosarcoma patients at Moewardi Hospital, Surakarta from 2018 to 2022. We described patients’ clinical presentations and assessed prognostic factors. Survival rate was calculated using Kaplan–Meier survival analysis. Prognostic factors were analyzed using univariate and multivariate Cox regression methods. Results Of the 82 patients included in the study, 40 (48.8%) were male and 42 (51.2%) were female. Most subjects (75.6%) were diagnosed with osteosarcoma at the age of >10 years, 45 patients (54.9%) had primary tumors in the femur, and 16 patients (19.5%) had limb salvage surgery. The mean event-free survival duration was 13.6 (95%CI 9.7 to 17.5) months. The mean duration of overall survival was 22.7 (95%CI 17.3 to 28.1) months, with a 44.1% survival probability. Type of surgery was a significant prognostic factor (P=0.018), with limb salvage having better survival probability (93.3%) than amputation (19.8%). Conclusion The overall survival rate of osteosarcoma patients at our center is still lower than in other regional centers. Limb salvage surgery was a significant prognostic factor for survival, while gender, location of primary tumor, histological subtype, and metastasis at the time of diagnosis were not.
Co-Authors Adi Magna, Adi Adi Prayitno Agus Kristiyanto Amarduan, Tince Bety Amilia Yuni Damayanti Ana Rima Asrial, An A. Bambang Purwanto Binti Maulina Budiningsih, Fatichati Budiyanti Wiboworini Cindikya Saftiari Dewi Cr. Siti Utari Cr. SitiUtari Darmayani, Aritantri David Perdana Dentiko Wasis Aulia Dewi Okta Anggraini Didik Prasetyo Diffah Hanim Dono Indarto ernawati, ammik Eti Poncorini Pamungkasari Fadila, F Gardjito, Fajar Baskoro Gunawan, Timotheus A. Hari Wujoso Harsono Salimo Hermansyah, Muhamad Lukman Ida Nurwati Idulhaq, Mujaddid Irma Suryani Jati, Agus Kusumaningrum, Sulistyani Laqif, Abdurrahman LAWEI, THARINA Marintan, Elita Muchamad Syamsulhadi, Muchamad Muhammad Riza Muthmainah Nanang Wiyono Nofia, Yasinta Nursulistyo, Fajar Paramasari Dirgahayu Paulus Kusnanto Pesik, Riza N. Poncorini, Eti Pudjiastuti, Anggit puguh santoso, puguh Rachmi Fauziah Rahayu Rahayu, Rachmi F. Rahmah Rahmah Ratih Puspita Febrinasari Reviono Reviono Risya Cilmiaty, Risya Riza Deviana Riza Novierta Pesik Sabariyah, Lely Santi, Nur Saputra, Rhyan Darma Setianto, Budi Y. Soestrisno, Soestrisno Soetrisno Soetrisno Soetrisno Suyatmi Suyatmi Tamaulina Br Sembiring Taufiqurrahman, Surya Tedjo Danudjo Tonang Dwi Ardyanto Tri Nugraha Susilawati Triyanta Yuli Pramana Umi Mahmudah Vitri Widyaningsih Wardhani, Lusi O. Wayanshakty, Janur Wulandari, Eka T. Wulansari, Indry Yarsa, Kristanto Y. Yudhani, Ratih D. Yundari, Yundari yusari Yusup Subagio Yusup Subagio Sutanto Zahidah, Fairuz Zaidah, Lailatuz