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All Journal Natural Science: Journal of Science and Technology METAMORFOSA Journal of Biological Sciences Microbiology Indonesia Pendidikan Ekonomi Pendidikan Biologi Jurnal Biologi Universitas Andalas Jurnal Riset Kimia agriTECH Bioeksperimen: Jurnal Penelitian Biologi Jurnal Litbang Industri Biopropal Industri Jurnal Biologi Tropis Jurnal Hilirisasi IPTEKS (JHI) Buletin Ilmiah Nagari Membangun Bioscientist : Jurnal Ilmiah Biologi Jurnal Litbang Industri
Periadnadi Periadnadi
Departemen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Andalas, Limau Manis, Padang, Sumatera Barat 25175
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Potensi Antimikroba dan Antioksidan Beberapa Ekstrak Daun Benalu (Scurrula ferruginea (Roxb. ex Jack) Danser dari Archidendron sp. terhadap Staphylococcus aureus, Escherichia coli, dan Candida albicans Apriyelita, Annisa; Periadnadi, Periadnadi; Nurmiati, Nurmiati
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.11108

Abstract

Scurrula ferruginea (Roxb. ex Jack) Danser is known for its role as an antimicrobial and antioxidant agent due to its content of phenolic compounds, alkaloids, and terpenoids. S. ferruginea has been widely used in traditional medicine for skin infections, diarrhea, hypertension, and digestive tract diseases. This study aims to determine the antimicrobial and antioxidant potential of various treatments of S. ferruginea mistletoe extracts. The experimental method used in this research is the nested pattern experiment. The extract treatments applied include fresh extract, boiled dry extract, boiled fresh extract, brewed dry extract, and brewed fresh extract. Antimicrobial potential was assessed using disc diffusion and dilution methods. Antioxidant activity (IC50) was determined using the DPPH (1,1-Diphenyl-2-Picryl-Hydrazine) method, and Total Phenolic Content (TPC) was also determined. The results indicate that the highest antimicrobial activity against Escherichia coli and Staphylococcus aureus was found in the strong category of fresh extract treatment, whereas there was no significant effect on Candida albicans growth. The fresh extract treatment exhibited the highest antimicrobial activity with a total phenolic content of 20.77321 mgGAE/mL. The Minimum Inhibitory Concentration (MIC) values for the fresh extract against S. aureus and E. coli were 6.25%, and the Minimum Bactericidal Concentration (MBC) values were 12.5% against both bacteria. The antioxidant value of the fresh extract was 101.26 μg/mL, categorized as moderate. Based on the conducted research, it can be concluded that S. ferruginea leaf extract can inhibit the activities of S. aureus and E. coli, while showing no effect on Candida albicans growth.
Eksplorasi Mikroflora Alami Produk Fermentasi Tradisional Cangkuak Berbasis Daging di Kecamatan Kuantan Mudik Kabupaten Kuantan Singingi Putri, Adela; Nurmiati, Nurmiati; Periadnadi, Periadnadi
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.11216

Abstract

Cangkuak is a fermented food made from meat with betung bamboo shoots or kluwak, salt and rice. The fermentation process is carried out without adding microbial cultures (spontaneous) for one to three weeks in a tightly closed container. cangkuak's manufacturing technique, ingredient formulation and constituent ingredients are unique, resulting in a final product with different characteristics. Research on the presence of microbes in fermented food products (fish, milk, vegetables) has been widely conducted, but information on exploring the presence of natural microflora and the in vitro potential of fermenting bacteria in cangkuak has never been reported. This research was conducted using a survey method and the resulting data were presented descriptively. It was found that the natural microflora found in the three samples of cangkuak in Kuantan Mudik was dominated by bacteria, with the highest proportion of bacterial groups in the three cangkuak samples being fermentative bacteria (2.0 - 6.30x105 cfu/g), cellulolytic bacteria (3.30 - 5.90x105 cfu/g), amylolytic bacteria (1.70 - 4.40x105 cfu/g), proteolytic bacteria (0.12 - 7.90x105 cfu/g), lipolytic bacteria (0.08 - 0.12x105 cfu/g). Six potential fermentative isolates were obtained as evidenced by the calculation of index values, namely DK-I1 (1.59), DR-I1 (1.36), DR-I2 (2.49), DR-I3 (1.19), DKR-I1 (2.41), and DKR-I2 (2.96).
Eksplorasi Mikroflora Alami Cairan Nepenthes mirabilis (Lour) Druce di Kawasan Hutan Penelitian dan Pendidikan Biologi Universitas Andalas Titami, Hania; Periadnadi, Periadnadi; Nurmiati, Nurmiati
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.11277

Abstract

Indonesia is a country that is rich in flora and fauna diversity, one of the unique flora in Indonesia is Nepenthes. This bag serves to trap insects or other small animals, the Biology Education and Research Forest (HPPB) is located in the Andalas Limau Manis University campus area which is classified as lowland tropical rain forest which is the habitat of Nepenthes, in the Biology Education and Research Forest (HPPB) this type of pitcher plant, namely Nepenthes mirabilis. Nepenthes mirabilis is a plant that is able to digest insects trapped in pockets at the ends of its leaf tendrils, so it is classified as a carnivorous plant. Microorganisms play an important role in breaking down organic materials into inorganic ones available to plants. Types of bacteria and bacterial populations greatly determine the decomposition process of animals that have been trapped in the bag as a source of nutrients for Nepenthes plants. This study aims to explore the natural microflora of Nepenthes mirabilis fluids, determine the proportional presence of potential bacteria, and determine the in vitro potential of chitinolytic bacteria from Nepenthes mirabilis fluids. This study used a survey method which was analyzed descriptively. The results showed that Nepenthes mirabilis fluid contained the presence of Fermentative, Amylolytic, Proteolytic, Chitinolytic and Lipolitical bacteria. Nepenthes mirabilis liquid in open bags contained chitinolytic bacteria, the highest presence of chitinolytic bacteria was found in MBB samples (20,104 cfu/ml) and MBA samples (18,104 cfu/ml). .28), fermentative (2.33 and 2.00), amylolytic (1.42 and 1.28), proteolytic (2.00 and 2.00), lipolytic (1.75 and 1.28).
Co-Authors Abdi Dharma Adela Putri Ando Fernando Annisa Apriyelita Ansofino Ansofino Anthoni Agustien Apriyelita, Annisa Awari Susanti Azizah Nurmalinda Dalli Yulio Saputra Dalli Yullio Saputra Deddi Prima Putra Desi Musliati Devi Syafriyani Diah Kharisma Sari Dina Wahyuna Dwisa Putra, Wahyu Efrizal Efrizal Eka Muliani Elisa Febriyanti Ervina Magdaulih Fauzan Azima Ferline Ariesta Fitri Wulandari Gustina Indriati Hafizatur Rahma Hania Titami Hezi Yolantika Ika Thalia Nissa Indra Anggriawan Intan Pramita, Intan Jadespi, Sherly K Kamsina Kamsina, K Kartika Indah Permata Sari Lara Annisa Lia Masefa, Lia Lusi Lusi Mades Fifendy Melisa Ainil Fajri Merla Pamericar Merry Tri Indah Permata Sari Mitra Angelia Mudinillah, Adam Nadia Merisya Nani Subandini Nasril Nasir Nasril Nasir Nilah Ratnasari, Nilah Nissa Arifa Novelina Nurmiati Nurmiati Nurmiati Nurmiati Nurmiati Nurmiati Nurmiati, N Pebriana Nasution Pujiana Okta Putri Putra, Wahyu Dwisa Putri, Adela Rahmadani, Silmi Yusri Ramza Seswati Risca Adelina Atro Risya Saputri, Risya Roza Ika Putri RRP. Megahati Sari, Diah Kharisma Sari, Wulan Komala Sekatresna, Widiyanti Sherly Jadespi Silmi Yusri Ramadani Sri Indrayati Suci Pebra Jusana Swandi, Monica Kharisma Syafrina, Vellin Putri Syovia Osnita Tesri Maideliza Titami, Hania Tivani Ardini Uswatul Hasana Yulia M. Nur Yuni Sepriani Yuniar Yuniar YUSRA YUSRA Yusra Yusra Zozy Aneloi Noli