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All Journal HAYATI Journal of Biosciences Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Kedokteran Brawijaya MANDALA of Health Research Journal of Life Science STOMATOGNATIC- Jurnal Kedokteran Gigi Insisiva Dental Journal : Majalah Kedokteran Gigi Insisiva Jurnal Fish Protech Amerta Nutrition E-Prodenta Journal of Dentistry Jurnal Impresi Indonesia
Nur Permatasari
Universitas Brawijaya
Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Journal : Research Journal of Life Science

 1 
Subchronic Toxicity of the Physalis minima Leaves Setyawati Soeharto; Dian Nugrahenny; Nur Permatasari; Elly Mayangsari
Research Journal of Life Science Vol 5, No 1 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (315.343 KB) | DOI: 10.21776/ub.rjls.2018.005.01.8

Abstract

The study was to evaluate the subchronic toxicity of aqueous extract of Physalis minima leaves in female rats. Eighteen female Wistar rats were orally administered with aqueous extract of P. minima leaves at doses of 90, 270 and 450 mg/kg BW, respectively for 90 days. Six female rats without any treatment were provided as a control group. At the end of the experiment, blood samples were collected for hematological and biochemical (aspartate transaminase/AST, alanine transaminase/ALT, creatinine, urea, glucose, cholesterol, and triglyceride) analyses. The results showed that hematological and biochemical biomarkers were not significantly different in treated groups compared to the control group. It is concluded that the aqueous extract of P. minima leaves do not induce hematologic toxicity, hepatotoxicity and renal toxicity in the subchronic toxicity study.
Effect of Histatin-1 Proteins to Fibroblasts Growth Factor-2 (FGF2) Concentrate and Fibroblasts Cell Migration on Human Gingival Fibroblast (HGF) Culture Dewi, Ni Ketut Ayu Feriyanti; Permatasari, Nur; Sujuti, Hidayat
Research Journal of Life Science Vol 8, No 2 (2021)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.02.4

Abstract

The purpose of this study is to determine the effect of histatin-1 protein increasing the concentration of fibroblast growth factor (FGF2) and fibroblast cell migration in human gingival fibroblast (HGF) culture. The HGF cell culture used was obtained from odontectomy patients and was the result of the 4th passage. Cell culture was divided into 4 groups: HGF control group (without treatment) and treatment groups with 5µM, 10µM, and 20µM concentration of histatin-1 respectively. The amount of fibroblast growth factor 2 (FGF2) was quantified by using ELISA method. In vitro fibroblast cells migration was measured using scratch/wound healing assay method. FGF2 concentration and HGF cell migration were measured at hour 0, 3, 11, 24, and 48. The results of the One Way Anova statistical test difference between FGF 2 (ΔFGF2) at hour 24 showed a value of P = 0.042 (P≤0.05) and HGF cell migration at hour 11 showed a P value = 0.003 (P≤0.05). The conclusion of this study is that Histatin-1 Protein is able to increase the concentration of Fibroblast Growth Factor 2 (FGF2) and the migration of Human Gingival Fibroblast cells.
Co-Authors Abdur Razaq Komaruzzaman Adinda Chika Anindita Agustin Iskandar Agustine Hanafi Putri Ahmad Reza Fadhlurrahman, Ahmad Reza Akhmad Sabarudin Amandia Dewi Permana Shita Anik Puryatni Annasari Mustafa Aswin D Baskoro, Aswin D Cahyani, Dwi Indah Citra Tarannita Damayanti, Elok Delfi Fitriyani Devitaningtyas, Nungky Dewi, Ni Ketut Ayu Feriyanti Dian Nugrahenny Dicky Faizal Irnandi Edi Widjajanto Edi Widjajanto Edwin Widodo Fardizia Putri Alia Ferdian Rizky Hutomo, Ferdian Fitriyani, Delfi Hafiedz Maulana Happy Kurnia P Hidayat Sujuti Ibrahim, Moh. Nuh Indriati Dwi Rahayu Joko Agus Gunawan Kartika Andari Wulan, Kartika Karyono Mintaroem Khadafi Indrawan Komaruzzaman, Abdur Razaq Lavina Sofia Ardani Loeki Enggar Fitri M Aris Widodo Mayangsari, Elly Meilia Aquina Meilia Aquina, Meilia Mohamad Nur Ibrahim Muhammad Nurul Amin Nathania Bella Claresta Niarti Ulan Sari Siarnu Noerpuspita, Putri Nour Athiroh Abdoes Sjakoer Nura Fattah Cantika Yoga Nuzulul Hikmah Putri Noerpuspita Putri, Agustine Hanafi Retty Ratnawati Retty Ratnawati Setyawati Soeharto Setyawati Soeharto Sidharta, Rudhanton Siti Nurfitria, Siti Sri Widyarti Stefania Widya Setyaningtyas Sudiarto Sudiarto Tika Ardhini Wardoyo