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Journal : JURNAL BIOLOGI INDONESIA

Pemurnian Parsial dan Karakterisasi Amilase dari Bakteri Laut Arthrobacter arilaitensis LBF-003 Rahmasari, Dianti; Wijanarka, Wijanarka; Pujiyanto, Sri; Rahmani, Nanik; Yopi, Yopi
JURNAL BIOLOGI INDONESIA Vol 12, No 1 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i1.2323

Abstract

Starch is an abundant carbon source in nature, and ?-amylase (1, 4-?-D-glucanohydrolase; EC 3.2.1.1), which hydrolyzes ?-1, 4-glucosidic linkage in starch-related molecules. Microbe ?-amylase production is a hydrolytic enzyme and one ofinterest in its microbial production has increased dramatically due to its wide spread use in food, textile, baking anddetergent industries in recent years. Here we report ?-amylase from marine bacterium which was purified andcharacterized, as well as analyzed its hydrolysis product on starch. The enzyme of Arthrobacter arilaitensis partiallypurified by acetone precipitation with 90% and ion exchange chromatography produced specific activity 0.25 U/mg and0.38 U/mg, and it’s purity rate increased until 1.14 fold compared with former crude extract. Purifed extracelluler amilasehad an optimum activity at temperature 50°C and pH 9.0. An apparent molecular mass was between 50-75 kDa, asestimated by zimogram electrophoresis. Hydrolysis products of this enzyme on starch were maltose, maltotriose andmaltoheptaose.Keywords: alfa amylase, marine bacterium, Arthrobacter arilaitensis, purification, charaterization
PEMURNIAN PARSIAL DAN KARAKTERISASI AMILASE DARI BAKTERI LAUT ARTHROBACTER ARILAITENSIS LBF-003 Rahmasari, Dianti; Wijanarka, Wijanarka; Pujiyanto, Sri; Rahmani, Nanik; Yopi, Yopi
JURNAL BIOLOGI INDONESIA Vol 12, No 1 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i1.2323

Abstract

Starch is an abundant carbon source in nature, and ?-amylase (1, 4-?-D-glucanohydrolase; EC 3.2.1.1), which hydrolyzes ?-1, 4-glucosidic linkage in starch-related molecules. Microbe ?-amylase production is a hydrolytic enzyme and one ofinterest in its microbial production has increased dramatically due to its wide spread use in food, textile, baking anddetergent industries in recent years. Here we report ?-amylase from marine bacterium which was purified andcharacterized, as well as analyzed its hydrolysis product on starch. The enzyme of Arthrobacter arilaitensis partiallypurified by acetone precipitation with 90% and ion exchange chromatography produced specific activity 0.25 U/mg and0.38 U/mg, and it?s purity rate increased until 1.14 fold compared with former crude extract. Purifed extracelluler amilasehad an optimum activity at temperature 50°C and pH 9.0. An apparent molecular mass was between 50-75 kDa, asestimated by zimogram electrophoresis. Hydrolysis products of this enzyme on starch were maltose, maltotriose andmaltoheptaose.Keywords: alfa amylase, marine bacterium, Arthrobacter arilaitensis, purification, charaterization
Co-Authors Achirul Nditasari Agung Suprihadi Agung Suprihadi Ahmad Qi Sahlan Akbar, Muhammad Falih Anang S. Achmadi Anggraeni, Via Annisa Widyasari Anto Budiharjo Aqlina, Maulida Ari Wibawa Budi Santosa Arina Tri Lunggani Ariyani, Mei Dwi Aviany, Hanna Berliana Bambang Sri Waluyo Budi Raharjo Budi Raharjo Bunga Fajriani Chang, Helen Chatarina Umbul Wahyuni Choirunnisaa, Nadia Maharani Jasmine Debby Widiyanti Dewanti, Ardelia Dewi, Tirta Kumala Dewi, Yulita Wiwik Irana Diani Ajeng Prahesti Dinda Khairunnisa, Dinda Dwi Retnowati Elawati, Nunung Eni Elawati, Nunung Eni Endang Kusdiyantini Endang Kusdiyantini Erfianti, Tia Fahrurrozi Fahrurrozi Fathmah, Ema Nuzula Fathmah, Ema Nuzulah Fatin, Nuhaul Fitri Ulfana Risky Fitria Fitria Gabriela Christy Sabbathini Genoveva Preta Angelika Ghaida Afra Akhsani Halwiyah, Nurul Hapzi Ali Hermin Pancasakti Kusumaningrum Hery Widijanto Hessy Novita Jepri Agung Priyanto, Jepri Agung Larasati, Dinar Rahmi Luthfian Nur Afifi M Muslikha Maerani Sumarno Mamik Setyowati Maulida Aglinia Mawarni, Shelina Nurunnisa MG Isworo Rukmi Moi, Maria Yasinta Mulyani, Nies S Nadina, Rahmah Qisti Nafisah, Hidayatun Nandina, Rahmah Qisti Nanik Rahmani NANIK RAHMANI Novi Alvita Pratama Noviasih, Ria Frasida Nunung Eni Elawati Nunung Eni Elawati Nurul Inayah Oktavia, Anik Oktavia, Nurrizqi Permatasari, Vera Pramita Dian Pramitasari Prastya, Muhammad Eka Pratama, Novi Alvita Primahana, Gian Putra, Mohammad Affan Dwica Putri Ramadhani Putri, Adde Lolita Octavia R. Rahardian Rahmah, Chairunnisaa Jabal Rahmandanni, Yunnia Rahmasari, Dianti Rahmasari, Dianti Rahmawati Dewi RATIH DEWI HASTUTI Rejeki Siti Ferniah Resdiani, Merysa Ridho Mathori Ikhwan Rifka A. N. Safitri Ristia Rachmatunnisa Riza Laksita Devi Mutiaratri Rizko, Nurmalisa Roseliana Fitri Roslenawati Roslenawati RS Ferniah Sari, Suli Arum Sarsa A. Nisa Setiawan, Ruby Sigit Hananto Siti Nur Jannah Siti Nur Jannah Sri Rahayu Tri Astuti Sugiyono Saputra Sumarno, Maerani Sunarno s Sunarno Sunarno Sunarno Sunarno Supriyadi Supriyadi Susiana Purwantisari T. A. Lestari Taufiq P. Nugraha Tri Rahayu, Hesti Tsania Dyna Falasifa Utami, Linda Ayu w wijanarka Wahyu Aji Mahardhika Wandita, Ryan Hilda Wigunarti, Anggia Hesti Wijanarka Wijanarka Wijanarka, W Wijarnaka, Wijarnaka Yopi Yopi YOPI YOPI Yudi Yunanto Yuswan, Apriza