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Seleksi dan Optimasi Karakter Fisik Bakteri Penghasil Fitase yang Diisolasi dari Sumber Air Panas di Guci, Tegal Purwati, Catur Suci; Sajidan, Sajidan; Ratriyanto, Adi; Nuhriawangsa, Adi Magna Patriadi
Sains Peternakan: Jurnal Penelitian Ilmu Peternakan Vol 13, No 2 (2015): Sains Peternakan
Publisher : Universitas Sebelas Maret (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/sainspet.v13i2.11482

Abstract

The aimed of this study to isolate, select and characterize physically phytase-producing bacteriafrom hot springs in the Guci, Tegal. Isolation and enrichment of bacteria using Lurya Betani mediawith 2% of phytic acid substrate. Extraction of extracellular phytase thus obtained extract coarse andoptimization by loking at the relative activity at the difference in the resulting product. Phytaseproducingbacteria can be isolated from the hot springs of the Guci, Tegal with the highest relativeactivity in AG2 and AG2-1 colonies. The highest relative activity at a temperature of 55°C, pH 6, theincubation time of 90 minutes, the substrate concentration of 3% and a metal cofactor Ca2+ at aconcentration of 10-4M. Phytase-producing bacteria can be isolated from a hot spring in Guci, Tegaland have certain physical characteristics.
EFFECT OF PROTECTED INDIAN SARDINE (Sardinella longiceps) OIL, PALM OIL, AND PALM KERNEL CAKE INTAKE ON DIGESTIBILITY OF DRY MATTER, ORGANIC MATTER, AND CRUDE PROTEIN BY RUMEN FLUID OF FISTULAE ONGOLE BREED CATTLE Susi Dwi Widyawati, Catur Suci Purwati Wara Pratitis S.S &
Proceeding of International Congress 2014: Challenges of Biotechnological Research in Food and Health
Publisher : Proceeding of International Congress

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (130.814 KB)

Abstract

Livestock business, ruminant in particular, has been growing rapidly with the increased demand ofhigh quality food ingredient, such as meat and dairy products. However, digestion process in ruminants isrelatively more complicated compare to other animals. Carbohydrate is the main energy source and fat asthe alternate, both from vegetable and animal’s fat. This study aimed to determine the effect of protectedIndian sardine fish oil, palm oil, and palm kernel cake consumption on digestibility of dry matter, organicmatter, and crude protein by rumen fluid of fistulae Ongole breed cattle. Latin square experiment designwas applied on 3 treatments. Fermented rice straw (FRS), basal concentrate (BC), and protected materialsof Indian sardine oil (ISO), palm oil (PO), and palm kernel cake (PKC) were used as feed ingredient.Treatments were: P1 = FRS 40% + BC 60 % (BC 95% + PO 5%) ; P2 = FRS 40% + BC 60% (BC 95 % + ISO5%) ; P3 = FRS 40% + BC 60 % (BC 90% + PKC 10%). Analyzed parameters were intake and digestibility ofdry matter, organic matter, and crude protein, and also digestible nutrient of organic matter (DN OM)and crude protein (DN CP). The results showed that average dry matter intake of P1, P2, P3 were 6108.77;5965.87; 5686.78 (g/head/day), organic matter intake were 5658.19; 5569.29; 5603.11 (g/head/day), proteinintake were 690.58; 829.84; 818.15 (g/head/day), dry matter digestibility were 54.55; 54.00; 54.23 (%),organic matter digestibility were 66.09; 66.59; 64.00 (%), crude protein digestibility were 81.77; 80.48;79.73 (%), DN OM were 61.33; 62.06; 63.06 (%), and DN CP were 10.69; 10.34; 10.82 (%), respectively.Analysis of variance showed no significant difference between intake and digestibility of dry matter,organic matter, crude protein, DN OM, DN CP (P > 0.05) among treatments. It was concluded thatconsumption of 5% Indian sardine oil, 5% palm oil, and 10% palm kernel cake protected materials did notaffect of dry matter, organic matter, and crude protein digestibility in fistulae Onggole Crossbreed rumen.Keywords: Ongole grade cattle, Indian sardine oil, palm oil, and palm kernel cake, digestibility
Fermentasi Biji Kecipir (Psophocarpus tetragonolobus) oleh Jamur Trichoderma Viride Terhadap Warna, Tekstur, dan Serat Kasar Purwati, Catur Suci; Windyasmara, Ludfia
Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science) Vol 9 No 1 (2019): Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veter
Publisher : Fakultas Peternakan Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (136.2 KB) | DOI: 10.30862/jipvet.v9i1.2

Abstract

The fermentation process is an activity of microorganisms that make products with characteristics of texture, flavor, aroma and changes in nutrient quality that better than the original raw material. It is also protein process of developing of protein from the material. This study uses a Completely Randomized Design with four treatments. P0 = Unfermented winged seeds, P1 = Winged seeds + 0,1% Trichoderma viride mushroom (Tv), P2 = Winged seeds + 0,2% Trichoderma viride mushroom (Tv), P3 = Winged seeds + 0,3% Trichoderma viride mushroom (Tv). The material used was crushed winged seeds as substrates that mixed to be homogenized. One hundred gr of each sample from every treatment was weighed, put into a perforated plastic bag (to create anaerobic atmosphere) with 2 cm thick. Subsequently, samples were incubated in fermented room at 30oC for 7 days. Each treatment was repeated 3 times. Variables observed in color, texture and coarse fiber. This study indicated that the winged fermented beans with Trichoderma viride mushroom had no effect on color, effected crude fiber and texture.
Perubahan Suhu, pH, Protein Kasar, dan Serat Kasar pada Fermentasi Biji Kecipir (Psophocarpus tetragonolobus) dan Tepung Jagung dengan Level Jamur Trichoderma viride yang Berbeda Catur Suci Purwati; Danang Riyadi
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 5, No 3 (2018): JITRO, September
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (168.81 KB) | DOI: 10.33772/jitro.v5i3.4766

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ABSTRAKTujuan penelitian ini adalah mengetahui proses fermentasi yang tepat digunakan untuk meningkatkan kualitas protein kasar dan serat kasar pada biji kecipir dan menentukan level terbaik penambahan jamur Trichoderma viride yang mampu meningkatkan kualitas protein kasar dan serat kasar pada biji kecipir sehingga mampu meningkatkan kualitas dan tingkat kecernaan. Produk yang akan dihasilkan pada penelitian ini adalah alaternatif pakan fermentasi biji kecipir (Psophocarpus tetragonolobus) untuk peternak unggas. Penelitian ini menggunakan rancangan acak lengkap dengan empat perlakuan yaitu P0 = biji kecipir dengan 10% tepung jagung tanpa fermentasi; P1 = Biji kecipir dengan 10% tepung jagung + 0,1% jamur Trichoderma viride (Tv); P2 = biji kecipir dengan 10% tepung jagung + 0,2% Tv; dan P3 = biji kecipir dengan 10% tepung jagung + 0,3% Tv. Materi yang digunakan adalah biji kecipir yang dihaluskan digunakan sebagai substrat dan dihomogenkan dengan cara diaduk. Masing-masing sampel perlakukan ditimbang sebanyak 100 g, kemudian dimasukkan ke dalam kantong plastik dengan ketebalan 2 cm  dan dilubangi agar tercipta suasana aerob. Selanjutnya diinkubasikan dalam ruang fermentor pada suhu 30oC selama 7 hari. Masing-masing perlakuan diulang sebanyak 3 kali. Peubah yang diamati pH, suhu, protein kasar dan serat kasar. Berdasarkan penelitian ini dapat disimpulkan bahwa terjadi perubahan suhu, penurunan pH, serta kenaikan pada protein kasar dan penurunan serat kasar.Kata kunci: biji kecipir, Trichoderma viride, pH, suhu, protein, serat kasar ABSTRACTThe purpose of this study was to determine the proper fermentation process used to improve the quality of crude protein and crude fiber in winged beans and the best level of addition of Trichoderma viride fungus which could improve the quality of crude protein and crude fiber in winged beans to improve feed quality and digestibility. The result product of this study is an alternative fermented winged bean (Psophocarpus tetragonolobus) feed for poultry farmers. This study utilized completely randomized design with four treatments. P0 = Unfermented winged beans with cornstarch, P1 = winged beans with 10% cornstarch + 0,1% Trichoderma viride fungus (Tv), P2 = winged beans with 10% cornstarch + 0,2% Tv, P3 = winged beans with 10% cornstarch + 0,3% Tv. The material used were powdered winged beans as substrate then homogenized by stirring. Each sample was weighed 100 g, then put into a plastic bag with a 2 cm thickness and perforated to create an aerobic atmosphere. Subsequently, the sample was incubated in a fermenter room at 30oC for 7 days. Each treatment was repeated 3 times. Variables observed for pH, temperature, crude protein, and crude fiber. The result of this study concluded that there were change in temperature, decrease in pH, increase in crude protein, and decrease in crude fiber.Keywords: winged beans, Trichoderma viride, pH, temperature, protein, crude fiber
Suplementasi Ekstrak Brokoli sebagai Agen Kyuring Alami terhadap Uji Sensorik dan Protein Terlarut Daging Sapi Kornet Catur Sucipurwati; Engkus Ainul Yakin; Sri Sukaryani
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 8, No 1 (2021): JITRO, Januari
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (81.318 KB) | DOI: 10.33772/jitro.v8i1.14415

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ABSTRAKBrokoli dapat digunakan sebagai alternatif untuk dipakai sebagai agen kyuring alami produk daging organik. Brokoli lebih cocok dan lebih sesuai sebagai penambah aroma dan cita rasa pada produk olahan daging dibandingkan dengan sayuran berhijau daun lainnya yang mengandung nitrat yang tinggi. Brokoli memiliki manfaat lain yaitu menormalkan kadar gula darah, menurunkan kadar kolesterol jahat, serta sebagai antioksidan. Tujuan suplementasi ekstrak brokoli sebagai agen kyuring alami pengaruhnya terhadap warna, tekstur, dan protein terlarut daging sapi kornet. Perlakuan pada penelitian ini terdiri atas P1 (0% ekstrak brokoli); P2 (5% penambahan ekstrak brokoli); P3 (10% penambahan ekstrak brokoli); P4 (15% penambahan ekstrak brokoli) dengan ulangan sebanyak empat kali. Data dianalisis menggunakan analisis ragam jika ada pengaruh maka dilakukan uji lanjut berganda Duncan. Hasil penelitian suplementasi ekstrak brokoli sebagai agen kyuring alami berpengaruh nyata terhadap warna dan tekstur, serta berpengaruh sangat nyata terhadap protein terlarut. Suplementasi brokoli 15% memberikan pengaruh yang terbaik.Kata kunci: ekstraksi brokoli, kyuring alami, daging sapi kornet Supplementation of Broccoli Extract as Natural Curing Agent on Sensory Test and Water Soluble Protein of Corned BeeABSTRACT Broccoli can be used as an alternative for natural curing agents for an organic beef product. broccoli is better than other high nitrate content vegetables to increase taste and aroma. Furthermore, it can normalize blood sugar, decrease blood cholesterol, and has antioxidant properties. This study aimed to determine the color, texture, and water soluble protein of beef cornet supplemented by broccoli extract. The treatments experimented were P1 (0% broccoli extract), P2 (5% broccoli extract), P3 (10% broccoli extract), and P4 (15% broccoli extract), each repeated 4 times. The data obtained were analysed by analyzed of variance continued by Duncan test. The results indicated that broccoli extract supplementation significantly affected color, texture, and water soluble protein. 15% broccoli extract supplementation showed the best result.Keywords: broccoli extraction, natural curing agent, corned beef
KONTRIBUSI EKSKRESI BASAL PURIN TERHADAP TOTAL EKSKRESI DERIVAT PURIN DALAM URIN KAMBING BLIGON DAN KEJOBONG Catur Suci Purwati; Lies Mira Yusiati; Subur Priyono Sasmito Budhi
Buletin Peternakan Vol 37, No 1 (2013): Buletin Peternakan Vol. 37 (1) Februari 2013
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v37i1.1953

Abstract

Penelitian ini bertujuan untuk mengetahui perbedaan kontribusi ekskresi basal terhadap total ekskresi derivat purin dalam urin kambing Kejobong dan Bligon. Percobaan ini didesain menggunakan Independent Student t-test untuk membandingkan ekskresi derivat purin keturunan kambing yang berbeda. Pakan yang diberikan adalah jerami kacang tanah (rendeng). Kambing jantan Bligon dan Kejobong masing-masing 6 ekor digunakan dalam penelitian ini. Percobaan ini dibagi menjadi 3 periode yaitu periode adaptasi selama 14 hari, periode koleksi pada saat ternak diberi pakan ad libitum selama 7 hari, dan periode koleksi pada saat ternak dipuasakan. Penentuan kandungan bahan kering (BK), bahan organik (BO), protein kasar (PK), serat kasar (SK), dan ekstrak eter (EE) dilakukan dengan metode analisis proksimat untuk pakan, sisa pakan, dan feses. Pada sampel urin yang diambil saat ternak diberi pakan ad libitum dan dipuasakan dilakukan pengukuran derivat purin yaitu alantoin, asam urat, xantin, dan hipoxantin. Hasil penelitian memperlihatkan konsumsi BK, BO, PK, SK, dan EE menunjukkan perbedaan yang tidak nyata. Nutrien tercerna BK, BO, PK, SK, dan EE juga menunjukkan perbedaan yang tidak nyata. Volume urin kambing Kejobong dan Bligon pada pemberian pakan secara ad libitum menunjukkan perbedaan yang nyata (P<0,05), dengan rerata kambing Kejobong 953,84 dan Bligon sebesar 762,69 ml/hari. Total ekskresi alantoin dan asam urat kambing Kejobong cenderung lebih tinggi daripada Bligon, sedangkan untuk total ekskresi xantin dan hipoxantin menunjukkan hasil yang berbeda tidak nyata. Total ekskresi derivat purin pada saat pakan ad libitum menunjukkan perbedaan yang nyata (P<0,05), rerata kambing Kejobong 117,96±16,43 μmol/W0,75/hari dan Bligon 72,40±4,53 μmol/W0,75/hari. Volume urin pada saat dipuasakan menunjukkan perbedaan yang tidak nyata. Rerata volume urin kambing Kejobong 233,04 dan Bligon 201,75 ml/hari. Total ekskresi alantoin kambing Kejobong pada saat dipuasakan cenderung lebih rendah dibanding Bligon, namun untuk total ekskresi asam urat cenderung lebih tinggi kambing Kejobong dibanding Bligon. Total ekskresi xantin dan hipoxantin menunjukkan perbedaan yang tidak nyata. Total ekskresi derivat purin kambing Bligon pada saat dipuasakan lebih tinggi dari kambing Kejobong, dengan rerata kambing Kejobong 18,85±4,45 μmol/W0,75/hari, dan Bligon sebesar 19,33±2,28 μmol/W0,75/hari. Derivat purin endogen kambing Kejobong 0,018 μmol/W0,75 sedangkan Bligon 0,019 μmol/W0,75. Efisiensi sintesis protein mikrobia kambing Kejobong adalah 0,07 g N mikrobia/hari, sedangkan Bligon 0,04 g N mikrobia/hari dengan BK nutrien tercerna hampir sama. Kontribusi ekskresi basal derivat purin terhadap total ekskresi derivat purin kambing Kejobong 15,98%, sedangkan Bligon 26,70%. (Kata kunci: Ekskresi, Derivat purin, Kambing Bligon, Kejobong)
PROTEKSI MINYAK IKAN LEMURU, MINYAK KELAPA SAWIT, DAN BUNGKIL SAWIT TERHADAP pH DAN NH3DALAM RUMEN SAPI PERANAKAN ONGOLE Catur Suci Purwati
Buletin Peternakan Vol 40, No 1 (2016): BULETIN PETERNAKAN VOL. 40 (1) FEBRUARI 2016
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v40i1.9819

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The aimed of this research is to know the influence of protection lemuru oil, palm oil, and palm oil cake to pH and NH3in the rumen of the Ongole. The material were used in this study werecomulated rumenfemale cows with average body weight of 289.33 ± 28.34 kg as many as 3 heads. Latin square experiment design was applied on 3 treatments. Fermented rice straw (FRS), basal concentrate (BC), and protected materials of Indian sardine oil (ISO), palm oil (PO), and palmkernel cake (PKC) were used as a feed ingredient.  Treatments were: P1 = FRS 40% + BC 60% (BC 95% + PO 5%); P2 = FRS 40% + BC 60% (BC 95% + ISO 5%) ; P3 = FRS 40% + BC 60% (BC 90% + PKC 10%). Parameters measured were pH and NH3. Latin square experiment design was applied on 3 treatments.Conclusions of this study are pH and NH3remain stable, meaning lemuru addition of fish oil, palm oil, and palm oil cake is protected not disturb the digestive process in the cow rumen fistulated onggole breedparticular.(Key word: Indian sardine oil, NH3, Palm kernel cake, Palm oil, pH, Protection)
Substitution of the Fermented Cocoa Pod Waste in to the Grass Based Diet on Performance of Bligon Goat Engkus Ainul Yakin; Sri Sukaryani; Catur Suci Purwati
Buletin Peternakan Vol 45, No 1 (2021): BULETIN PETERNAKAN VOL. 45 (1) FEBRUARY 2021
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v45i1.61589

Abstract

This experiment was aimed to evaluate the effect of fermented cocoa pod as feed for Bligon goats. Sixteen Bligon goat with a body weight of 11-13 kg were put into individual cages which were equipped with feed and drink containers. The study design used a randomized block design, where initial body weight in as peragam with four treatments and four replications. Fermented cocoa pod used Trametes versicolor. The experimental treatments were T1= 30% of fresh king grass + 50% of dried king grass + 20% of concentrate; T2= 30% of fresh king grass + 30% of dried king grass + 40% of concentrate; T3= 30% of fresh king grass + 30% of cocoa pod + 40% of concentrate; and T4= 30% of fresh king grass + 30% of cocoa pod fermented + 40% of concentrate. Observed variables were feed intake, body weight gain and feed conversion. The result showed that fermented cocoa pod at the level of 30% had higher (P<0.05) infeed intake (560.33 g day-1), body weight gain (101.79 g head-1day-1), and feed conversion (5.50) compared to other treatments. The conclusion of this study were the use of 30% cocoa pod fermented in the ration showed the best body weight gain and feed conversion on Bligon goat performance. 
IBM PEMANFAATAN LIMBAH RUMAH PEMOTONGAN HEWAN (RPH) JUNGKE KARANGANYAR SEBAGAI BAHAN PEMBUATAN PUPUK ORGANIK DI KELOMPOK TANI RUKUN MAKARYO MOJOGEDANG, KARANGANYAR Catur Rini Sulistyaningsih; Catur Suci Purwati
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2017: Prosiding Seminar Nasional Publikasi Hasil-Hasil Penelitian dan Pengabdian Masyarakat
Publisher : Universitas Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (467.311 KB)

Abstract

Penelitian IbM ini bertujuan untuk  penanganan limbah ternak yaitu pembuatan starter-mol berbasis rumen ternak  sekaligus sebagai pupuk hayati dan pembuatan pupuk organik padat berbasis kotoran padat ternak (feses) dan sisa pakan ternak sapi di tambah dengan sekam (limbah padi). Target dari IbM ini adalah mengurangi pencemaran limbah khususnya cairan rumen pada RPH, meningkatkan nilai tambah cairan rumen yang dapat digunakan untuk sumber mikroorganisme pada pembuatan stsrter, meningkatkan pengetahuan mengenai pembuatan biofertilizer dan pupuk organik yang berkualitas, produksi pupuk organik yang berkualitas agar mencukupi kebutuhan pupuk pada lahan pertanian kelompok tani Rukun Makaryo. Meningkatkan hasil produksi beras organik, yang dihasilkan dari kelompok tani kelompok tani Rukun Makaryo. Metode yang dilakukan yaitu dengan penyuluhan dengan ceramah, diskusi, melalui pelatihan dengan praktek langsung oleh mitra, pendampingan kepada mitra dan evaluasi. Keywords: RPH, pupuk organik, kelompok tani Rukun Makaryo
ISOLASI, SELEKSI DAN OPTIMASI BAKTERI PENGHASIL FITASE DARI TANAH VULKANIK DI GUCI, TEGAL Adi Magna Patriadi Nuhriawangsa; Adi Ratriyanto; Sajidan Sajidan; Catur Suci Purwati
Sains Peternakan: Jurnal Penelitian Ilmu Peternakan Vol 14, No 1 (2016): Sains Peternakan
Publisher : Universitas Sebelas Maret (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/sainspet.v14i1.8772

Abstract

The purpose of this study was to isolated, selected and characterized phytase bacteria derived from volcanic soil in the Guci, Tegal. Bacteria from the volcanic soil was isolated and selected on Luria Betany (LB) media containing 4% phytic acid. Selected colonies were optimized by physical character with determining the relative activity. Colonies of bacteria could be isolated from the volcanic soil, selected, and had the highest relative activity in colonies of TG3 and TG3-5. TG3-5 bacterial colonies producing phytate had the highest optimum value at a temperature of 55°C, pH 7, the incubation time of 90 minutes, the substrate concentration of 5% and a best metal cofactor on Zn2+ (10-4M). Volcanic soil in the Guci, Tegal containing phytase-producing bacteria with certain physical characteristics.A