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All Journal Jurnal Pengendalian Hayati Agrovigor: Jurnal Agroekoteknologi Plumula : Berkala Ilmiah Agroteknologi Agrifor : Jurnal Ilmu Pertanian dan Kehutanan Jurnal Ilmu-Ilmu Pertanian Indonesia Jurnal Agroekoteknologi Jurnal Perlindungan Tanaman Indonesia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Ilmiah Universitas Batanghari Jambi BIOEDUSCIENCE Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) JPT : JURNAL PROTEKSI TANAMAN (JOURNAL OF PLANT PROTECTION) JURNAL AGROHITA Jurnal Pembelajaran dan Biologi Nukleus Jurnal Agrotropika PLAKAT : Jurnal Pelayanan Kepada Masyarakat Nusantara Science and Technology Proceedings Bioscientist : Jurnal Ilmiah Biologi Berkala Penelitian Hayati Jurnal Pengabdian Kepada Masyarakat JPMP Agrocentrum Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Yenny WURYANDARI
Program Studi Agroteknologi, Fakultas Pertanian, UPN “Veteran” Jawa Timur
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Social Sciences Other

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Eksplorasi dan Uji Antagonis Bakteri Endofit Dari Daun Tanaman Mangrove Avicennia sp. Terhadap Jamur Patogen Alteria sp. Secara In Vitro Perwira Moekti, Nirwana Septania Galih; Prasetyawati, Endang Triwahyu; Wuryandari, Yenny
Jurnal Agroekoteknologi Vol 17, No 1 (2025)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/jur.agroekotetek.v17i1.30972

Abstract

Mangrove (Avicennia sp.) merupakan salah satu ekosistem penting yang sering ditemukan di muara sungai yang memiliki kemampuan untuk tumbuh dan berkembang di zona salinitas tinggi. Salah satu jenis tanaman mangrove yang bermanfaat dan terdapat berbagai jenis bakteri endofit adalah Avicennia marina. Oleh karena itu, penelitian ini bertujuan untuk mendapatkan isolat bakteri endofit yang berpotensi sebagai agensia hayati pada daun tanaman bakau Avicennia sp. terhadap cendawan patogen Alternaria sp. Metode isolasi yang digunakan adalah penanaman langsung. Pengujian antagonis dilakukan dengan metode dual culture melalui pengukuran diameter zona hambat. Sampel yang diambil adalah daun yang sehat dan segar. Bakteri endofit yang diperoleh sebanyak 24 isolat bakteri yang memiliki morfologi yang berbeda. Diameter zona hambat dengan kategori kuat adalah isolat D28 sebesar 12,5 mm dengan kategori kuat dan kategori sedang adalah isolat D13, D19 sebesar 7,5 mm; 8,5 mm.  Mekanisme kerja bakteri endofit adalah antibiosis dan kompetisi ruang. Hasil penelitian ini dapat disimpulkan bahwa terdapat bakteri endofit dari daun mangrove Avicennia sp. yang berpotensi sebagai antijamur terhadap jamur patogen Alternaria sp.
Potensi Konsorsium Bakteri Pseudomonad fluorescent dan Bacillus mycoides Terhadap Penyakit Layu Bakteri Secara In-vitro Sagala, Yosua Nathanael Itona; Prasetyawati, Endang Triwahyu; Wuryandari, Yenny
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 8 No. 2 (2024): December 2024
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.8.2.78-87.2024

Abstract

Bacterial wilt disease caused by Ralstonia solanacearum is a disease in chili pepper plants (Capsicum annuum) that is difficult to control. One technique to prevent bacterial wilt disease is consortium of antagonistic bacteria such as Pseudomonad fluorescent and Bacillus mycoides. This study aimed to determine whether the consortium of Pseudomonad fluorescent pf-142 and Bacillus mycoides can inhibit R. solanacearum better than a single application in-vitro. This study was conducted in a Completely Randomized Design (CRD) with four treatments (Pseudomonad fluorescent pf-142, B. mycoides, Pseudomonad fluorescent pf-142 + B. mycoides, and control) in six replications, resulting in 24 experimental units. The variables observed were the symptoms of attack and pathogenicity of R. solanacearum, compatibility and inhibitory rate of the consortium of Pseudomonad fluorescens pf-142 and B. mycoides against R. solanacearum. Based on the study, it was known that R. solanacearum caused wilting in chili pepper plants with high virulence. Pseudomonad fluorescent pf-142 and B. mycoides did not produce an inhibition zone, indicating that both were compatible. The consortium of Pseudomonad fluorescent pf-142 and B. mycoides provided the widest inhibition zone, indicating strong antagonistic ability against R. solanacearum.
EVALUATING THE LONG-TERM STORAGE TIME VIABILITY AND SIZE DYNAMICS OF BACILLUS SP. BIOENCAPSULATION IN SODIUM ALGINATE MATRIX Saputra, Mochammad Mirza; Wuryandari, Yenny; Rahmadhini, Noni; Lestari, Safira Rizka
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 2 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2023.2549

Abstract

The use of biological agents such as Bacillus sp. bacteria has begun to be widely used by farmers as a new form of control. Bacillus sp. needs to require special methods in its application because it has certain living conditions, and Bacillus sp. is vulnerable to environmental pressures. Bioencapsulated formulation in the form of granules is considered effective as a form of bacterial application in soil because it is able to protect Bacillus sp. and maintain survival. This study aims to determine the best concentration of sodium alginate in maintaining the viability of Bacillus sp. at the in vitro stage and to see changes in the size of the beads. The beads were made using extrusion method by combining Bacillus sp. suspension and sodium alginate suspension at 1%, 1.5%, and 2% concentration. The concentration of sodium alginate was not a major factor in changes in bead size and viability test results experienced significant changes in each observation. 1.5% and 2% concentrations can reach the highest viability value of 3x106 CFU/mL and decreased during 1 month of observation. Changes in bead size and viability were caused by the alginase enzyme produced by Bacillus sp.
EFFECT OF GROWING MEDIA ON THE GROWTH OF Streptomyces sp. AND Trichoderma harzianum AS ANTHRACNOSE DISEASE BIOCONTROL Rulinggar, Nia; Mujoko, Tri; Wuryandari, Yenny
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.9257

Abstract

Streptomyces sp. and Trichoderma harzianum is a biological agent that is effective in controlling Colletotrichum capsici, the cause of disease in chili plants. Different growing media can affect the growth and activity of these two microorganisms. This study aims to evaluate the growth of Streptomyces sp. and Trichoderma harzianum on various media, namely rice washing water, coconut water, and sugar potato extract. The research phase includes growing media test, effectiveness test of Streptomyces sp. and Trichoderma harzianum in chili peppers, and effectiveness test of Streptomyces sp. and Trichoderma harzianum on chili seeds. The results showed that the medium of rice washing water is more suitable for Streptomyces sp., coconut water media is more suitable for Trichoderma harzianum, and rice washing water media is more suitable for the combination of the two biological agents. Optimal growth in these media affect the effectiveness of biocontrol in suppressing the severity of anthracnose disease in chili.
Rebranding Produk Kopi Excelsa Oleh Kelompok Masyarakat KOPI GUNUNG Dengan Packaging Baru dan Digital Marketing Zainal Abidin Achmad; Yenny Wuryandari; Kusuma Wardhani Mas’udah; M. Husni Tamrin
Plakat : Jurnal Pelayanan Kepada Masyarakat Vol 4, No 2 (2022): Plakat: Jurnal Pelayanan Kepada Masyarakat
Publisher : Fakultas Ilmu Sosial dan Ilmu Politik, Universitas Mulawarman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/plakat.v4i2.8899

Abstract

Coffee is a leading commodity in Jombang Regency. One of these coffee varieties is Excelsa Coffee. The main obstacle for coffee farmers and traders in Sumberjo Village is the processing of excelsa coffee which is quite complicated. The Grant Program for Application of Research Results for the Community (PIHAT) aims to foster community groups KOPI GUNUNG in Sumberjo Village, Wonosalam District, Jombang Regency to have knowledge and skills in coffee plant care and digital marketing. The results of the identification of the problem of Pokmas KOPI GUNUNG as a partner, the PIHAT team provides solutions in the form of a) Increasing knowledge and skills in caring for coffee plants, b) Improving the quality of new labels and packaging, c) Creating Instagram accounts and making marketing support profile videos, and d) Digital marketing training. After partners gain knowledge and skills in caring for coffee plants, knowledge of digital marketing skills, and producing coffee product packaging, the goal of implementing this PIHAT program is to increase sales of coffee products from the KOPI GUNUNG community group with the brand and packaging "Cakra Dewa." In addition to articles in community service journals, PIHAT's outputs are the Profile Video, news, and Instagram accounts. Kopi merupakan komoditas unggulan Kabupaten Jombang. Salah satu varietas kopi tersebut adalah Kopi Excelsa. Kendala utama bagi petani dan pedagang kopi di Desa Sumberjo adalah perawatan kopi excelsa yang cukup rumit. Program Hibah Penerapan Hasil Penelitian Bagi Masyarakat (PIHAT) bertujuan untuk membina kelompok masyarakat KOPI GUNUNG di Desa Sumberjo, Kecamatan Wonosalam, Kabupaten Jombang agar memiliki pengetahuan dan keterampilan dalam perawatan tanaman kopi dan pemasaran digital. Hasil identifikasi masalah Pokmas KOPI GUNUNG sebagai mitra, maka tim PIHAT memberikan solusi berupa: a) Meningkatkan pengetahuan dan keterampilan dalam merawat tanaman kopi, b) Meningkatkan kualitas label dan kemasan baru, c) Membuat akun Instagram dan membuat video profil penunjang pemasaran, dan d) Pelatihan pemasaran digital. Setelah mitra mendapatkan pengetahuan dan keterampilan dalam merawat tanaman kopi, pengetahuan keterampilan digital marketing, dan memproduksi kemasan produk kopi, maka sasaran akhir dari pelaksanaan program PIHAT ini adalah meningkatkan penjualan produk kopi dari kelompok masyarakat KOPI GUNUNG dengan brand dan kemasan “Cakra Dewa.” Selain artikel pada jurnal pengabdian kepada masyarakat, luaran PIHAT ini adalah Video Profil, Berita, dan akun Instagram.
Optimization of Bioencapsulation of Bacillus sp. to Increase Resistance to Bacterial Wilt and Growth of Cayenne Pepper Ajeng Distya Anjani; Yenny Wuryandari; Safira Rizka Lestari
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol. 14 No. 4 (2025): August 2025
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtepl.v14i4.1325-1334

Abstract

Cayenne pepper has a high production demand. The high demand does not match the amount of production produced. One of the factors for decreased production is the presence of bacterial wilt disease caused by Ralstonia solanacearum. Bacterial wilt control can be done with the application of antagonistic bacteria, namely Bacillus sp. Bacillus sp. application requires an optimal formulation to maintain bacterial activity in the soil, one of which is Bacillus sp. encapsulation. This study aims to determine the effectiveness of Bacillus sp. encapsulation as a formulation of biological agent bacteria for the control of bacterial wilt disease and cayenne pepper plant growth. This study used the encapsulating material concentration and dosage factors for application to plants. Encapsulation was made using the extrusion method. The results showed that the concentration of 2% sodium alginate and 1.5% gelatin gave a significant difference in disease intensity of 18.89% and in the growth parameters of cayenne pepper plants were able to reach a height of 30.58 cm and a root length of 17.18 cm. The right concentration and high dose provide the ability to suppress intensity and encourage maximum growth
The Potential for Controlling Maize Pest Spodoptera frugiperda (Lepidoptera: Noctuidae) Using Biological Agents Bacillus spp. Muhammad Qoyyimul Amri; Yenny Wuryandari; Noni Rahmadhini
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol. 15 No. 2 (2026): April 2026
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtepl.v15i2.608-617

Abstract

Maize production is still facing many challenges nowadays.  One of the main challenges in maize production is pest attacks.  S. frugiperda has been causing significant damage to maize farming in Indonesia recently.  This research was conducted to determine the potential of Bacillus spp. isolates Bcz-20 and Bcz-30 are identified as biological agent candidates to control the S. frugiperda population, with bacterial population densities of 109 CFU/mL, 108 CFU/mL, and 107 CFU/mL, respectively, as determined by in vitro and in vivo application tests.  The Bcz-20 treatment with a population density of 107 CFU/mL was capable of suppressing the S. frugiperda larvae population in an in vitro application test, resulting in a mortality percentage of 47.5%. The Bcz-20 treatment with a population density of 109 CFU/mL was capable of suppressing the S. frugiperda larvae population by in vivo application test, resulting in a mortality percentage of 70 %.  The conclusion, Bacillus spp. Bcz-20 isolates with a population density of 107 CFU/mL are efficient and effective for controlling the S. frugiperda larvae population.
Potential of Bacillus spp. Isolates as Biological Control Agents Against Rhizoctonia solani, the Causal Agent of Sheath Rot in Maize (Zea mays L.) Salma Alfiani Nadiva; Yenny Wuryandari; Tri Mujoko
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 2 (2026): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i2.20689

Abstract

This study aimed to evaluate the effectiveness of laboratory-collected Bacillus spp. isolates against Rhizoctonia solani, the causal agent of maize sheath blight, under both in vitro and in vivo conditions, as available information on their efficacy remains limited. The in vitro assay was conducted using the dual culture method with five Bacillus spp. isolates, namely Bcz-14, Bcz-16, Bcz-20, Bcz-21, and Bcz-30, to assess their inhibitory activity against R. solani. The results showed that all isolates were able to inhibit pathogen growth, although the percentage of inhibition varied among isolates. Isolate Bcz-14 exhibited the highest mean inhibition, while isolates Bcz-16 and Bcz-21 were selected for the in vivo assay based on a combination of mean inhibitory activity and consistency across replications. The in vivo assay was conducted using the two selected isolates with different application times, namely seven days before planting and at planting. The results indicated that treatment B1T1, consisting of Bcz-16 applied seven days before planting, showed a greater tendency to suppress disease development, as indicated by a longer incubation period and the lowest disease intensity of 17.78%. These findings suggest that isolate Bcz-16 has potential for further development as a biological control candidate for maize sheath blight. However, further evaluation under field conditions is required to confirm its effectiveness.
Co-Authors Achmad Fiqri Agus Wahyudi Aisyah Lulu Hariyanto Ajeng Distya Anjani Annisa Zukhruf Oktavia Salamah arfah Arika Purnawati Bambang Hadisutrisno Bambang HADISUTRISNO Budi Rianto Devi Tria Anjarsari Devi Tria Anjarsari Didiek Tranggono Edo Pandu Pratama Erlyana Desy Rahmawati Fadia Nur Rahma Budiarto Frida Nur Aisah Gita Pawana Hera Amalia Putri Belangi Herry Nirwanto HIDAYATI, KIKI I. Hartana Indah Sari Dwi Agustin Indarwati Indarwati Kaswan Badami Kiki Sandra Amelia Kusuma Wardhani Mas’udah Lestari, Safira Rizka Lukmanul Hakim M. Husni Tamrin Maroeto ., Maroeto Maroeto Maroeto Mochammad Mirza Saputra Mochammad Mirza Saputra Muhammad Qoyyimul Amri Munir, Ahmad Arsyad Nauval Zakqy Nirwana Septania Galih Perwira Moekti Nolla Dwi Elvira Noni Rahmadhini Noni Ramadhini Nova Andriani Novita Endah Ariani Novita Endah Ariani Nuriatus Sholikhah Penta SURYAMINARSIH Perwira Moekti, Nirwana Septania Galih Prasetyawati, Endang Triwahyu Putranto Sasikirono Putri, Ratih Nadhila Raharja, Wildan Taufik Ratu Yusnita Zhalsabilla Rulinggar, Nia Safira Rizka Lestari Sagala, Yosua Nathanael Itona Salma Alfiani Nadiva Saputra, Mochammad Mirza Saputra, Mochammad Mirza Setiawan Setiawan Shelomitha Kumala Mawardhany Sherly Arindawati Sri Wiyatiningsih Sulistyanto Suwandi Syamsul Arifin Taufik Hidayat Tr Mujoko Tri MUJOKO Triwidodo Arwiyanto Triwidodo ARWIYANTO Wahyu, Endang Tri Windriyanti, Wiwin Yovita Nathania Yuwono, F. Tsara Nafisah Zainal Abidin Achmad Zakqy, Nauval