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All Journal Biogenesis: Jurnal Ilmiah Biologi Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) KLOROFIL: Jurnal Ilmu Biologi dan Terapan Indonesian Journal of Chemical Analysis (IJCA) J-Dinamika: Jurnal Pengabdian Kepada Masyarakat Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) BIO-EDU: Jurnal Pendidikan Biologi Unri Conference Series: Community Engagement Jurnal Pengabdian Kepada Masyarakat (JPKM) Tabikpun KLOROFIL: Jurnal Ilmu Biologi dan Terapan Jurnal Pengabdian Masyarakat Indonesia Sriwijaya Bioscientia NEAR: Jurnal Pengabdian kepada Masyarakat Gunung Djati Conference Series AMMA : Jurnal Pengabdian Masyarakat
Kusuma Handayani
Department Of Biology, FMIPA Universitas Lampung
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Public Health Social Sciences Transportation Other

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Turmeric-Associated Endophytic Bacteria with IAA-Producing and Phosphate-Solubilizing Activities as Growth-Promoting Agents Siti Sholekha; Ahmad Ikhsanudin; Indriyani Indriyani; Kusuma Handayani; Nida Lidya Susanti; Ahmad Bintang Pamela
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 9, No 2 (2025): KLOROFIL: JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v9i2.27641

Abstract

White turmeric (Curcuma zedoaria) is one of the strategic bio-pharmaceutical plants commonly used as a cooking ingredient and as a family medicinal plant. In the Lampung region, turmeric ranks among the top five highest-yielding bio-pharmaceutical crops, making it an abundantly available commodity. This research aims to explore the endophytic bacteria present in turmeric and to investigate the functional abilities of the isolated strains. Endophytic bacteria are microorganisms that inhabit plant tissues and can act as plant growth promoters. In addition, these bacteria can solubilize phosphate, thereby supporting plant growth. Endophytic bacteria also produce the phytohormone IAA, which plays an important role in plant development. This study obtained three endophytic bacterial isolates from the leaves of white turmeric plants, namely KPD1, KPD2, and KPD3. Isolates KPD2 and KPD3 exhibited phosphate-solubilizing activity, as indicated by the formation of clear zones measuring 2.5 ± 0.58 mm and 4.0 ± 0.82 mm, respectively. In addition, all isolates were able to produce indole-3-acetic acid (IAA), with concentrations of 54.98 µg/mL (KPD2), 54.14 µg/mL (KPD3), and 25.05 µg/mL (KPD1). The abilities demonstrated by these endophytic bacterial isolates indicate their potential as plant growth-promoting bacteria (PGPB).
Turmeric-Associated Endophytic Bacteria with IAA-Producing and Phosphate-Solubilizing Activities as Growth-Promoting Agents Siti Sholekha; Ahmad Ikhsanudin; Indriyani Indriyani; Kusuma Handayani; Nida Lidya Susanti; Ahmad Bintang Pamela
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 9, No 2 (2025): KLOROFIL: JURNAL ILMU BIOLOGI DAN TERAPAN
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v9i2.27641

Abstract

White turmeric (Curcuma zedoaria) is one of the strategic bio-pharmaceutical plants commonly used as a cooking ingredient and as a family medicinal plant. In the Lampung region, turmeric ranks among the top five highest-yielding bio-pharmaceutical crops, making it an abundantly available commodity. This research aims to explore the endophytic bacteria present in turmeric and to investigate the functional abilities of the isolated strains. Endophytic bacteria are microorganisms that inhabit plant tissues and can act as plant growth promoters. In addition, these bacteria can solubilize phosphate, thereby supporting plant growth. Endophytic bacteria also produce the phytohormone IAA, which plays an important role in plant development. This study obtained three endophytic bacterial isolates from the leaves of white turmeric plants, namely KPD1, KPD2, and KPD3. Isolates KPD2 and KPD3 exhibited phosphate-solubilizing activity, as indicated by the formation of clear zones measuring 2.5 ± 0.58 mm and 4.0 ± 0.82 mm, respectively. In addition, all isolates were able to produce indole-3-acetic acid (IAA), with concentrations of 54.98 µg/mL (KPD2), 54.14 µg/mL (KPD3), and 25.05 µg/mL (KPD1). The abilities demonstrated by these endophytic bacterial isolates indicate their potential as plant growth-promoting bacteria (PGPB).
Antibacterial Activity Test of Methanol Extract of Gracilaria sp. against Escherichia coli Using Disc Diffusion Method: Uji Aktivitas Antibakteri Ekstrak Metanol Gracilaria sp. terhadap Escherichia coli Dengan Metode Difusi Cakram Ammanda Sadiva Ajeng Pawesti; Kusuma Handayani; Endah Setyaningrum; Hendri Busman
BIO-EDU: Jurnal Pendidikan Biologi Vol. 10 No. 3 (2025): BIO-EDU: Jurnal Pendidikan Biologi - December 2025
Publisher : Jurusan Pendidikan Biologi, Fakultas Keguruan dan Ilmu Pendidikan, Universitas Timor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32938/jbe.v10i3.9389

Abstract

The utilization of marine resources as an alternative source of natural antibacterial agents has been increasingly developed in recent years. Gracilaria sp., a type of red algae, is known to contain biologically active compounds with potential antibacterial properties, particularly against Escherichia coli. This study aims to determine the antibacterial potential of methanol extract from Gracilaria sp. against E. coli using an experimental method with a Completely Randomized Design (CRD). Data were analyzed using One-Way ANOVA followed by Tukey's test. The stock solution of the extract was prepared at a concentration of 100 mg/mL and tested at concentrations of 0.05%, 1%, 5%, and 10%, with ciprofloxacin as the positive control and sterile distilled water as the negative control. The experiment was conducted with three replications. The results showed that the methanol extract of Gracilaria sp. formed inhibition zones at the highest concentrations of 10% and 5%, with an average diameter of only 0.075 mm. Thus, the methanol extract of Gracilaria sp. exhibits weak antibacterial potential against Escherichia coli, as the inhibition zone formed was significantly smaller than that of the positive control, which reached 1.7 mm.
Co-Authors Achmad Arifiyanto Agustrina, Rochmah Ahmad Bintang Pamela Ahmad Ikhsanudin Ammanda Sadiva Ajeng Pawesti Atang Sutandi Bambang Irawan Berliana Damayanti C. Nugroho Ekowati Chistina Nugroho Ekowati Christina Ekowati Christina Nugroho Ekowati Cindy Lukyta Ratih Riyanto Cristina Nugroho Ekowati DWI ANDREAS SANTOSA Dzul Fithria Mumtazah Dzul Fitrhia Eko Prihadi Endah Setyaningrum Endah Setyaningrum Endah Setyaningrum Eti Ernawiati Fadlina Athfin G. Nugroho Susanto Gina Dania Pratami Hendri Busman Indriani Indriani Indriyani Indriyani Indriyani Jihan Fikra Angelia M Kanedi M. Kanedi Meishy Handerlin Putri Mica Mirani Mohamad Kanedi Mohamad Kanedi Nida Lidya Susanti NISA RACHMANIA MUBARIK Nismah Nukmal Nuning Nurcahyani Nuri Oktavia Oktaviantimala Oktaviantimala Primasari Pertiwi Rochmah Agustrina Rochmah Agustrina Salman Farisi Siti Sholekha Sumardi, Sumardi Untung Sudadi Vidia Royanti Wawan Abdullah Wawan Abdullah S Wawan Abdullah Setiawan Wawan Setiawan Abdullah Yasmin Fadia Yulianty Yusifa Arsy Variani